##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	3291_5903_10010_H94MGADXX_HK_CF2_TGACCA_R2.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	23172864	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27379602279632	34.0	31.0	34.0	28.0	34.0
2	31.471154234539156	34.0	31.0	34.0	29.0	34.0
3	31.526196200866668	34.0	31.0	34.0	28.0	34.0
4	34.90603505030712	37.0	35.0	37.0	33.0	37.0
5	34.88499915245694	37.0	35.0	37.0	33.0	37.0
6	34.85451478936743	37.0	35.0	37.0	32.0	37.0
7	34.84734597329014	37.0	35.0	37.0	32.0	37.0
8	34.830365033860296	37.0	35.0	37.0	32.0	37.0
9	36.5243501623278	39.0	38.0	39.0	33.0	39.0
10-14	36.79310586727648	39.4	38.2	39.4	33.0	39.4
15-19	37.86066972990477	41.0	39.0	41.0	33.0	41.0
20-24	37.80975602325202	41.0	39.0	41.0	33.0	41.0
25-29	37.660221265701125	41.0	39.0	41.0	32.2	41.0
30-34	37.49572463723085	41.0	38.0	41.0	32.0	41.0
35-39	37.18512090693666	40.2	38.0	41.0	31.0	41.0
40-44	37.01158427374363	40.0	38.0	41.0	30.8	41.0
45-49	36.600911531695004	40.0	37.0	41.0	30.0	41.0
50-54	36.265977679755075	40.0	36.4	41.0	29.6	41.0
55-59	35.89553659832466	39.6	35.0	41.0	28.4	41.0
60-64	35.331172426507145	38.8	35.0	41.0	28.0	41.0
65-69	34.69782055424828	37.4	34.8	40.2	26.6	41.0
70-74	33.97280896310443	36.4	34.0	39.2	26.0	41.0
75-79	33.061738134742434	35.2	34.0	37.6	25.6	39.8
80-84	32.22786807880114	35.0	33.0	36.4	24.0	38.4
85-89	31.820670694826497	35.0	33.8	35.8	24.0	37.0
90-94	31.38308871100266	35.0	33.2	35.0	22.2	36.0
95-99	31.293212457467497	35.0	34.0	35.0	21.6	36.0
100-101	30.88932606690308	35.0	33.5	35.0	16.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	582970.0
3	84552.0
4	52445.0
5	43154.0
6	40958.0
7	40381.0
8	42340.0
9	45729.0
10	47866.0
11	50131.0
12	50442.0
13	50554.0
14	50884.0
15	51025.0
16	51219.0
17	51689.0
18	52173.0
19	53720.0
20	55464.0
21	57484.0
22	61614.0
23	72107.0
24	85836.0
25	101385.0
26	120736.0
27	146174.0
28	175996.0
29	213236.0
30	260792.0
31	327021.0
32	412784.0
33	531891.0
34	757515.0
35	1189759.0
36	2373070.0
37	4460766.0
38	7290992.0
39	3021014.0
40	14996.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.406125650648438	21.71889614570351	16.417776539578043	34.45720166407001
2	20.554023120684615	22.62505438208239	31.255652313051236	25.56527018418176
3	19.621610159406274	22.56935209720324	31.042363415127532	26.76667432826296
4	23.27799799375681	26.1861045649182	24.124251433307016	26.411646008017975
5	25.20283987708918	29.217254675743288	25.35001298052515	20.229892466642383
6	21.01784416367852	35.0558348522522	26.664531969892995	17.261789014176287
7	17.38589071381074	25.849183928213794	40.472351199354065	16.292574158621402
8	18.59047054818948	27.629715772235393	33.68021562952769	20.099598050047447
9	18.81150804203453	25.685070961466334	35.03429119046254	20.469129806036598
10-14	19.32733296185831	31.38152804811392	29.91302222149062	19.378116768537147
15-19	20.35660157518343	30.688732116363337	29.44324226290828	19.51142404554495
20-24	19.247876118759503	30.35595669735635	30.452302512898317	19.94386467098582
25-29	19.68070983363285	29.940887808757534	29.933719447842655	20.44468290976696
30-34	18.99948084299232	30.852523711399876	30.28334051248996	19.864654933117844
35-39	19.311526754357477	30.943080662407734	29.823498919571918	19.921893663662868
40-44	19.516296085369948	30.813401607657738	30.237527061179915	19.4327752457924
45-49	19.532255422489094	30.641855693817387	30.369616456384502	19.456272427309017
50-54	18.93225654777366	31.09581327245643	30.499533858346545	19.472396321423364
55-59	18.976290920196032	30.551325034466455	31.135644402754625	19.33673964258289
60-64	19.404935831164096	30.877038585278427	30.64837091455811	19.06965466899937
65-69	18.980882468141054	30.990625185671977	30.583383822863407	19.44510852332356
70-74	18.97212601546404	31.037769129520033	30.897558462933006	19.092546392082923
75-79	19.180319687734055	31.005449630784664	30.091849237877504	19.722381443603776
80-84	19.125183005509946	31.125951453026573	30.841677198487066	18.907188342976415
85-89	19.628724939132553	30.787698940617553	30.21212999753456	19.371446122715337
90-94	19.153579146585574	30.670945943499405	30.639354401867863	19.53612050804716
95-99	19.842519276811522	30.647741133392532	30.383741531561643	19.125998058234305
100-101	19.825737979508297	30.388751447733426	31.008496062523676	18.777014510234597
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	61.863327314718454
2	46.119293304866375
3	46.38828448766923
4	49.68964400177479
5	45.432732343731566
6	38.27963317785481
7	33.67846487243214
8	38.69006859823693
9	39.28063784807112
10-14	38.70544973039546
15-19	39.868025620728375
20-24	39.191740789745324
25-29	40.125392743399814
30-34	38.86413577611017
35-39	39.23342041802035
40-44	38.94907133116235
45-49	38.98852784979811
50-54	38.40465286919702
55-59	38.31303056277892
60-64	38.474590500163465
65-69	38.425990991464616
70-74	38.064672407546965
75-79	38.90270113133783
80-84	38.03237134848636
85-89	39.00017106184789
90-94	38.68969965463273
95-99	38.968517335045824
100-101	38.6027524897429
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10749.0
1	7137.0
2	3155.0
3	2856.5
4	2965.0
5	3109.0
6	3249.0
7	3290.5
8	3482.0
9	3834.0
10	4302.0
11	5095.0
12	5934.5
13	6816.5
14	7746.0
15	9120.0
16	12268.5
17	15318.0
18	21335.5
19	34409.0
20	49048.5
21	63544.0
22	85026.0
23	121605.0
24	178874.5
25	236725.0
26	306246.5
27	388595.5
28	474067.5
29	575822.0
30	702285.0
31	834164.0
32	946107.5
33	1023780.5
34	1058560.5
35	1045612.0
36	1009971.5
37	1011219.0
38	1015739.5
39	993310.5
40	986802.0
41	921630.5
42	822448.0
43	780014.5
44	874236.0
45	807714.0
46	661867.5
47	629287.5
48	506609.0
49	428803.5
50	388277.5
51	343025.5
52	370188.0
53	363038.0
54	278703.5
55	240730.0
56	214771.5
57	189788.5
58	171302.5
59	157142.0
60	141236.0
61	120661.0
62	100565.5
63	83760.0
64	69557.0
65	55524.0
66	42885.5
67	33538.5
68	25865.0
69	19468.5
70	14955.0
71	11334.0
72	8434.5
73	6234.5
74	4772.5
75	3992.0
76	3172.0
77	2362.0
78	1735.5
79	1350.0
80	1075.5
81	748.5
82	522.0
83	355.0
84	255.0
85	186.5
86	128.0
87	95.0
88	74.5
89	60.5
90	51.5
91	37.5
92	31.5
93	27.5
94	30.5
95	29.0
96	19.5
97	20.0
98	24.0
99	32.5
100	97.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3383397063047537
2	0.10423830217965288
3	0.09826579916923518
4	0.10410884040919585
5	0.11500952148167788
6	0.13552058131442016
7	0.15028785393121888
8	0.14957149880135662
9	0.1183064812359836
10-14	0.11779726493885262
15-19	0.11602622791900043
20-24	0.12579368696074858
25-29	0.11525549884554623
30-34	0.1492452551398049
35-39	0.2868320463107193
40-44	0.1558866439642506
45-49	0.233061394569096
50-54	0.33639777974789825
55-59	0.13539716109325114
60-64	0.10199947662921596
65-69	0.10287981666832378
70-74	0.09980380500226471
75-79	0.1070234564014185
80-84	0.1164128870734321
85-89	0.11082100166815807
90-94	0.1265592375633845
95-99	0.1161703620234426
100-101	0.11738298727339011
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.3172864E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	84.40089115112305
#Duplication Level	Relative count
1	100.0
2	76.15756035578144
3	63.00635324015248
4	43.34688691232529
5	27.715374841168995
6	18.17789072426938
7	13.24015247776366
8	10.452350698856417
9	8.406607369758577
10++	147.75603557814486
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCT	156718	0.6762996580828334	No Hit
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATA	137922	0.5951875434991548	No Hit
GATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGA	94192	0.40647543609628917	No Hit
GATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCT	77393	0.33398116003270034	No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAG	66861	0.2885314478175853	No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTT	59600	0.25719738397463515	No Hit
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGC	47641	0.20558960687811398	No Hit
TCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGA	43031	0.1856956481512169	No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGAT	25938	0.1119326467371491	No Hit
CTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT	23441	0.10115711204277554	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	2388195	3.7501304	9.967601	10-14
AAAAA	20622330	3.5492043	4.3416257	65-69
TTTTT	18119865	3.1749835	4.242848	95-97
CCCCC	2074405	3.036577	12.608274	1
GCAGG	1976725	1.9669305	11.859514	4
GGGGA	1899915	1.9172294	5.8499823	15-19
GGCCG	1239100	1.8918583	9.288178	25-29
CGGCC	1237960	1.8637674	9.103401	25-29
TGCAG	2874805	1.8449011	8.169329	3
CTGCA	2872515	1.8177322	9.230598	2
GGCCC	1197850	1.8033812	34.447353	9
CCCCA	1854780	1.7694719	10.886289	1
GCGGC	1156995	1.7665005	8.738608	20-24
CTGGG	1717235	1.7148697	5.164394	1
GCCGC	1131500	1.7034903	8.869117	25-29
CGGGG	1053450	1.631148	8.304113	10-14
TGGCC	1645845	1.6206646	23.55618	8
CCTCC	1673385	1.6021595	5.4868855	1
GCCCA	1621055	1.5683615	5.1635866	1
CGCGG	1019780	1.5570005	8.386023	20-24
CCTGG	1580950	1.5567626	5.04937	1
CAGGA	2420445	1.5477517	7.44542	5
TCTGC	2411150	1.5312654	7.2380004	1
CCCAG	1577830	1.5265416	6.18334	1
CTGGA	2369995	1.5209402	18.118704	2
GCCCC	980320	1.4553108	8.310058	1
CCGGG	939475	1.4343908	7.6209726	10-14
CCCGG	946165	1.4244658	7.687745	10-14
CCCCT	1483780	1.4206249	8.321901	1
CCCTG	1447980	1.4059491	5.9500694	1
ACTGG	2190345	1.40565	16.909056	1
CTCCA	2251205	1.4047059	5.1463513	1
ATGCA	3328755	1.3728085	12.25127	6
CTCCC	1411690	1.3516033	7.008748	1
ATGGC	2105010	1.3508867	15.449282	7
TGCAT	3237420	1.3399411	11.609896	7
GCCCG	852230	1.283045	7.5077252	10-14
CTCCT	2045990	1.2812461	6.463061	1
TCTGT	2997975	1.2452984	5.1911607	9
TCCTT	2921100	1.1964501	6.1175714	3
GCATC	1841765	1.1654719	16.51944	8
CCCCG	784795	1.165049	7.1871552	1
CATCT	2849655	1.1630055	11.131097	9
GATGC	1800630	1.1555512	17.237217	5
CCTAC	1820780	1.1361295	6.230683	4
GGATG	1737570	1.1308478	17.781359	4
GTTCC	1750015	1.1113938	5.7071214	9
GATCC	1690835	1.069963	13.7700615	1
TGGAT	2329725	0.97788674	12.26007	3
ATCCT	2326565	0.9495214	8.483317	2
TCGCG	937640	0.9232947	5.670171	20-24
TATGG	2015135	0.8458397	9.684713	6
CTACG	1312405	0.830492	5.509676	5
AGTAT	2901945	0.7827739	6.4596767	4
CGTTC	1223375	0.776937	5.451186	8
CCTTA	1851760	0.75574315	5.071609	4
GTATG	1797520	0.7544971	9.852701	5
TAGTA	2378030	0.6414526	6.178138	3
CTAGT	1379905	0.5711312	9.524755	2
ACTAG	1339845	0.552564	9.382269	1
>>END_MODULE