##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	3291_5903_10012_H94MGADXX_HK_CF70_GCCAAT_R1.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	11952919	
Filtered Sequences	0	
Sequence length	101	
%GC	38	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.742049536184425	34.0	31.0	34.0	30.0	34.0
2	31.831978364448048	34.0	31.0	34.0	30.0	34.0
3	31.931275113635422	34.0	31.0	34.0	30.0	34.0
4	35.254202258042575	37.0	35.0	37.0	33.0	37.0
5	35.031511214959295	37.0	35.0	37.0	33.0	37.0
6	34.755902303027405	37.0	35.0	37.0	32.0	37.0
7	34.84415212719169	37.0	35.0	37.0	32.0	37.0
8	34.85835526870047	37.0	35.0	37.0	32.0	37.0
9	36.523165512959636	39.0	37.0	39.0	33.0	39.0
10-14	36.742768557203476	39.4	37.8	39.4	32.6	39.4
15-19	37.83815611901996	41.0	38.4	41.0	33.0	41.0
20-24	37.777825801379564	41.0	39.0	41.0	33.0	41.0
25-29	37.63292486128283	40.8	38.6	41.0	32.0	41.0
30-34	37.450497673413494	40.0	38.0	41.0	32.0	41.0
35-39	37.3107858925506	40.0	38.0	41.0	31.2	41.0
40-44	37.10653971636551	40.0	38.0	41.0	31.0	41.0
45-49	36.8056341886028	40.0	37.4	41.0	30.0	41.0
50-54	36.468353613037955	40.0	36.6	41.0	30.0	41.0
55-59	36.07883680965294	39.6	35.6	41.0	29.0	41.0
60-64	35.72487619133033	39.0	35.0	41.0	29.0	41.0
65-69	35.11575465373772	37.8	35.0	40.6	28.0	41.0
70-74	34.35656441744481	36.6	34.8	39.4	27.6	41.0
75-79	33.46557350551777	35.6	34.0	38.0	26.0	40.0
80-84	32.69105740614489	35.0	34.0	36.6	26.0	38.6
85-89	32.035217004315015	35.0	34.0	35.8	25.0	37.0
90-94	31.588657046868633	35.0	33.8	35.0	24.6	36.0
95-99	31.24002742760994	35.0	33.0	35.0	22.4	36.0
100-101	30.724234013465665	35.0	32.5	35.0	17.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	246377.0
3	66538.0
4	37679.0
5	25582.0
6	21866.0
7	21116.0
8	20982.0
9	22053.0
10	22962.0
11	24276.0
12	24538.0
13	24524.0
14	24527.0
15	24911.0
16	25099.0
17	25150.0
18	25108.0
19	25760.0
20	26688.0
21	27862.0
22	30264.0
23	35263.0
24	42147.0
25	51278.0
26	62548.0
27	76492.0
28	99344.0
29	105486.0
30	125940.0
31	156004.0
32	196855.0
33	263689.0
34	376484.0
35	579716.0
36	1112119.0
37	2339409.0
38	3888742.0
39	1641248.0
40	6293.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.567815649271452	20.466557587257878	16.439540641285493	36.52608612218518
2	19.463543588687067	22.286041160573934	32.26359678371333	25.986818467025667
3	18.614707493890677	22.871418544426433	29.895775839649062	28.61809812203383
4	21.755182076673428	26.24971275540083	24.779321669263933	27.21578349866181
5	24.808014420292057	29.898805364473187	25.789947793648277	19.503232421586482
6	21.370358659425406	35.306102876806115	26.11469874287001	17.208839720898467
7	16.909577330007643	25.17367125894083	41.64530243643	16.271448974621528
8	17.91579264266014	27.697192876001107	34.35929854382912	20.027715937509626
9	18.429077015700695	25.747566365109453	35.7584401641906	20.06491645499925
10-14	19.04968874148233	32.10706669732609	30.14566112336118	18.6975834378304
15-19	19.69852267272585	30.75248993948629	30.216469250075818	19.33251813771205
20-24	19.16866391271818	30.672834638402314	30.526545175134434	19.631956273745075
25-29	19.45786468932273	30.21168729047853	30.418112288196646	19.91233573200209
30-34	18.984917199289164	30.845800744943467	30.580889482333678	19.588392573433687
35-39	19.233741092502616	30.895382663031732	30.201397913809963	19.669478330655686
40-44	19.23541289440469	30.804481032616195	30.560090251631884	19.400015821347235
45-49	19.386282062684927	30.781991522605146	30.62297447803628	19.20875193667365
50-54	19.08852980900223	30.900420678034795	30.68283245722058	19.328217055742392
55-59	18.967998499172705	30.733291051307198	31.141652691514913	19.157057758005188
60-64	19.18522404656895	31.01593716504718	30.780716088863898	19.018122699519967
65-69	18.927847848460914	31.05118574923868	30.865952826724346	19.15501357557606
70-74	18.995758043811286	31.049737686444594	30.924667729218264	19.029836540525856
75-79	19.06928940242076	31.00424224997569	30.44636791221698	19.480100435386568
80-84	18.937172566431883	30.961810118104637	31.01008764104951	19.09092967441397
85-89	19.267293710974528	30.759652793474373	30.888504024622137	19.08454947092896
90-94	19.041455680157586	30.81214934238808	30.824486834458565	19.321908142995767
95-99	19.31919067009069	30.86293481517273	30.642216508597443	19.17565800613914
100-101	19.564388224046937	30.697479310076243	30.834794427648287	18.903338038228533
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	63.09390177145663
2	45.45036205571273
3	47.232805615924505
4	48.97096557533524
5	44.311246841878535
6	38.579198380323874
7	33.18102630462917
8	37.94350858016976
9	38.49399347069994
10-14	37.74727217931273
15-19	39.031040810437894
20-24	38.800620186463256
25-29	39.370200421324824
30-34	38.57330977272285
35-39	38.903219423158305
40-44	38.63542871575192
45-49	38.59503399935858
50-54	38.416746864744624
55-59	38.12505625717789
60-64	38.203346746088926
65-69	38.08286142403697
70-74	38.02559458433714
75-79	38.54938983780733
80-84	38.028102240845854
85-89	38.35184318190349
90-94	38.36336382315336
95-99	38.49484867622983
100-101	38.46772626227547
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	476.0
1	313.5
2	136.0
3	144.5
4	170.5
5	175.5
6	191.5
7	214.0
8	280.0
9	367.5
10	433.5
11	563.0
12	760.5
13	962.5
14	1247.0
15	1619.5
16	2559.5
17	3786.5
18	6225.0
19	10695.5
20	15427.5
21	21887.0
22	31792.0
23	47530.0
24	71762.0
25	99774.0
26	136597.0
27	181728.0
28	231969.5
29	296652.5
30	372594.5
31	446597.0
32	517770.0
33	574423.5
34	602596.0
35	597966.5
36	578381.5
37	575823.5
38	562575.0
39	550502.5
40	551892.0
41	515881.5
42	459968.5
43	418928.5
44	418613.0
45	389286.0
46	343538.5
47	313397.5
48	257692.5
49	225188.5
50	202110.5
51	172800.0
52	168947.5
53	159695.5
54	130385.5
55	111983.0
56	96553.5
57	81626.5
58	69168.5
59	61754.0
60	54177.0
61	43828.5
62	34640.5
63	28079.0
64	22398.0
65	16828.0
66	12954.0
67	10389.0
68	8020.5
69	5987.0
70	4620.0
71	3594.5
72	2659.0
73	1892.5
74	1629.0
75	1615.0
76	1410.5
77	967.0
78	660.5
79	627.5
80	490.0
81	263.0
82	144.0
83	76.5
84	51.5
85	26.0
86	16.5
87	9.5
88	5.0
89	4.0
90	3.0
91	2.5
92	2.5
93	1.5
94	0.5
95	0.5
96	0.5
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03200891765434034
2	0.2258193166037518
3	0.01950151255940076
4	0.026545816967386796
5	0.0397894439006907
6	0.7265421944212958
7	0.002576776434275176
8	0.06832640629456285
9	0.018188025870500755
10-14	0.026283119629606794
15-19	0.027031054088126925
20-24	0.012930732651999064
25-29	0.018695015000101647
30-34	0.023460378172059895
35-39	0.019812733609254778
40-44	0.016541566122885966
45-49	0.011461635438172049
50-54	0.010549724297470768
55-59	0.009293127477898913
60-64	0.013461147021911552
65-69	0.00786418781889177
70-74	0.014118726982086969
75-79	0.009999231150148344
80-84	0.013399237458230915
85-89	0.01222797544265129
90-94	0.006132393267284753
95-99	0.010159861369427836
100-101	0.0010039388704968218
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.1952919E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	72.17843582721275
#Duplication Level	Relative count
1	100.0
2	44.19089167610498
3	21.764056098549997
4	13.118559223681082
5	9.13489100493589
6	7.297565613770153
7	5.972006655760169
8	5.000209740341457
9	4.31505795824769
10++	68.86055063830977
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATA	32672	0.273339089807268	No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCT	28987	0.24250980032576144	No Hit
GATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGA	17944	0.1501223257682914	No Hit
CTCCTGTTCACTTTTCTTCCCCAATTCGAGGCTCTACACGCAAATGGCTG	17602	0.14726109998737547	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC	17453	0.1460145425565086	TruSeq Adapter, Index 6 (100% over 50bp)
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGC	15369	0.12857947083888044	No Hit
GATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCT	15221	0.1273412795652677	No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTT	14291	0.11956075331891734	No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAG	13584	0.11364588014024023	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	916520	2.9506269	9.314832	1
CCCCC	765985	2.2488577	13.391532	1
GCAGG	854390	1.710347	7.316632	4
GGGGA	813415	1.6586181	5.0348263	2
TGCAG	1303995	1.6493447	5.0010357	3
CCCCA	868020	1.6441424	10.977383	1
GGCCC	535845	1.6322744	17.113039	9
CTGCA	1301375	1.6159647	6.201997	2
CCTGG	776380	1.5220956	5.538975	1
CCCAA	1241155	1.516714	5.3738413	2
GCCCA	779910	1.504736	5.9257545	1
TGGCC	758965	1.4879533	12.107091	8
CCTCC	764200	1.4439838	6.1461368	1
CCCAG	736010	1.4200367	6.4595976	1
CCTGC	730945	1.4068446	5.0606174	1
CCCCT	743980	1.4057775	9.595471	1
GCCCC	469865	1.4051443	7.7568707	1
CCCTG	727835	1.4008589	6.781808	1
CCAGT	1125080	1.3970528	5.6718807	3
CTGGA	1093995	1.3837285	8.521821	2
ACTGG	1093620	1.3832542	7.230093	1
ATGGC	1081045	1.3673489	7.980058	7
CTCCA	1117060	1.3617575	5.670142	1
CGGGG	425910	1.346121	5.242442	1
ATGCA	1650125	1.3219476	6.1385746	6
TGCAT	1628500	1.3014598	5.2666054	7
CCCAC	674330	1.2772684	5.2998705	1
CTCCC	670895	1.2676806	8.243854	1
CTCCT	1011510	1.2300962	8.351829	1
TCCTG	983815	1.2186767	6.3403435	2
CCTGT	948980	1.1755258	6.837055	3
CCCGG	380705	1.1596917	5.7414746	1
GATGC	872050	1.1030037	7.5914335	5
CCCCG	368310	1.1014413	8.03172	1
GCATC	873055	1.0841043	6.841208	8
GGATG	835935	1.0769963	7.931936	4
CCTCG	494435	0.9516355	5.4939566	1
CTCGG	484800	0.950452	5.747759	1
TGGAT	1147760	0.93432987	5.727342	3
GATCC	732655	0.90976447	5.4761405	1
CCCGC	301325	0.9011209	5.355935	1
>>END_MODULE