##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	3291_5903_10012_H94MGADXX_HK_CF70_GCCAAT_R2.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	11952919	
Filtered Sequences	0	
Sequence length	101	
%GC	38	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.955229262408622	34.0	31.0	34.0	28.0	34.0
2	31.16573566674383	34.0	31.0	34.0	28.0	34.0
3	31.219800954059842	34.0	31.0	34.0	28.0	34.0
4	34.57625555732454	37.0	35.0	37.0	32.0	37.0
5	34.561123605037395	37.0	35.0	37.0	32.0	37.0
6	34.54033303496828	37.0	35.0	37.0	32.0	37.0
7	34.53437658198805	37.0	35.0	37.0	32.0	37.0
8	34.516819615359225	37.0	35.0	37.0	32.0	37.0
9	36.17105980555879	39.0	37.0	39.0	32.0	39.0
10-14	36.42484562975788	39.4	37.2	39.4	32.0	39.4
15-19	37.46348464337456	41.0	38.2	41.0	32.0	41.0
20-24	37.41545200799904	41.0	38.6	41.0	32.0	41.0
25-29	37.273388667655155	41.0	38.0	41.0	31.0	41.0
30-34	37.098046862025924	40.0	38.0	41.0	30.6	41.0
35-39	36.82854661693934	40.0	38.0	41.0	30.0	41.0
40-44	36.7255782792471	40.0	38.0	41.0	30.0	41.0
45-49	36.36426794157979	40.0	37.0	41.0	29.0	41.0
50-54	36.04724618312899	40.0	36.4	41.0	28.2	41.0
55-59	35.69279597728387	39.6	35.4	41.0	27.6	41.0
60-64	35.14321766925719	39.0	35.0	41.0	26.4	41.0
65-69	34.50818709639043	37.6	34.6	40.2	26.0	41.0
70-74	33.75355343744904	36.4	34.0	39.2	25.4	41.0
75-79	32.83543870748225	35.2	33.8	38.0	23.2	39.8
80-84	31.99044636711752	35.0	33.0	36.4	21.4	38.4
85-89	31.547838415034857	35.0	33.0	35.8	20.6	37.0
90-94	31.105885215151215	35.0	33.0	35.0	19.0	36.0
95-99	31.001017809959222	35.0	34.0	35.0	12.8	36.0
100-101	30.6214248586475	35.0	33.0	35.0	2.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	368843.0
3	51379.0
4	32237.0
5	24625.0
6	22749.0
7	22068.0
8	23061.0
9	24096.0
10	25352.0
11	26656.0
12	26782.0
13	27074.0
14	27773.0
15	27992.0
16	27651.0
17	27760.0
18	28133.0
19	28184.0
20	29043.0
21	30134.0
22	32643.0
23	38910.0
24	46048.0
25	54804.0
26	64414.0
27	77348.0
28	93637.0
29	113357.0
30	138850.0
31	174035.0
32	217310.0
33	277915.0
34	387567.0
35	589691.0
36	1105337.0
37	2297792.0
38	3815885.0
39	1520920.0
40	4864.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.90673154266751	20.111924029197894	16.08385773237659	35.897486695758005
2	20.997910516086364	21.927001812103228	32.049344951943716	25.025742719866695
3	20.265751656603577	22.50336194679875	29.43470051665698	27.796185879940687
4	23.376425965057507	25.740774039264014	24.171443019562325	26.711356976116157
5	24.74855341943143	29.784989836265414	25.751207155496974	19.71524958880618
6	21.322658965386797	35.22406556140032	26.04000282800218	17.41327264521071
7	16.94836488250024	25.046779818463726	41.634159911536614	16.370695387499428
8	17.932782039018207	27.746589932503223	34.220628648437525	20.09999938004104
9	18.2657891563036	25.798838175385935	35.76428997373499	20.17108269457547
10-14	19.022910147309336	32.15395445589028	30.165853656575532	18.657281740224853
15-19	19.63779712368225	30.84655121636752	30.23198803908931	19.28366362086092
20-24	19.19562803185564	30.720353580640392	30.49702878838213	19.586989599121836
25-29	19.386301844194275	30.440759036328828	30.33739639888042	19.83554272059648
30-34	18.959552881022077	31.024445359756243	30.510644789128484	19.5053569700932
35-39	19.132252921548346	31.048511010747948	30.22293247933348	19.59630358837023
40-44	19.30468370339338	30.927368244658567	30.42969417679095	19.3382538751571
45-49	19.366878899022378	30.87126615767485	30.50431789275805	19.257537050544727
50-54	18.991887633435564	31.12587994546272	30.67357327157599	19.208659149525726
55-59	18.903240924398613	30.912974716229115	31.06319806011718	19.120586299255095
60-64	19.14561281331566	31.31527713950562	30.58239266779223	18.956717379386493
65-69	18.893824937291694	31.157712566557304	30.81900859036557	19.129453905785425
70-74	18.992089389379146	31.07977280042034	30.88270551468343	19.045432295517088
75-79	19.109931381500136	31.105152240486987	30.33494335841248	19.449973019600396
80-84	19.045585510913618	30.996407692082073	30.917161738212904	19.040845058791405
85-89	19.30396119919179	30.834791263617316	30.7762133112484	19.085034225942493
90-94	19.130888114876	30.860233994689683	30.692196527797496	19.31668136263682
95-99	19.430378423751627	30.915182699250167	30.53785410056888	19.116584776429335
100-101	19.70321864897934	30.714254186809903	30.820245133482842	18.76228203072791
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	63.80421823842551
2	46.02365323595306
3	48.061937536544264
4	50.087782941173664
5	44.46380300823761
6	38.735931610597504
7	33.31906026999967
8	38.03278141905925
9	38.43687185087907
10-14	37.680191887534185
15-19	38.921460744543175
20-24	38.78261763097748
25-29	39.22184456479075
30-34	38.46490985111527
35-39	38.72855650991857
40-44	38.64293757855048
45-49	38.6244159495671
50-54	38.20054678296129
55-59	38.023827223653704
60-64	38.10233019270215
65-69	38.02327884307712
70-74	38.037521684896234
75-79	38.55990440110054
80-84	38.08643056970502
85-89	38.38899542513428
90-94	38.44756947751282
95-99	38.54696320018096
100-101	38.465500679707255
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4537.0
1	2835.5
2	978.5
3	861.0
4	925.0
5	889.5
6	786.0
7	760.0
8	820.0
9	889.0
10	929.5
11	1063.5
12	1277.0
13	1499.5
14	1816.5
15	2198.0
16	3198.5
17	4464.0
18	6940.5
19	11474.0
20	16486.0
21	23176.5
22	33540.0
23	50170.5
24	75333.5
25	103630.5
26	139591.0
27	184492.5
28	234659.5
29	298391.0
30	373595.0
31	447258.5
32	517201.5
33	573129.0
34	601262.5
35	595875.5
36	575767.0
37	571394.0
38	557932.5
39	547526.5
40	547628.5
41	509735.0
42	454719.0
43	414502.0
44	412765.0
45	383743.5
46	344113.5
47	315638.0
48	257015.5
49	223799.0
50	200302.0
51	171836.0
52	166789.5
53	157367.0
54	129853.5
55	112405.5
56	97270.5
57	82270.0
58	69908.5
59	62458.5
60	55148.0
61	45036.5
62	35847.0
63	29274.0
64	23570.0
65	17977.5
66	14037.5
67	11275.0
68	8679.0
69	6525.0
70	5133.0
71	4007.5
72	3013.5
73	2264.0
74	1832.5
75	1687.5
76	1477.5
77	1080.0
78	839.0
79	791.5
80	639.5
81	383.5
82	226.5
83	140.0
84	99.0
85	72.5
86	50.0
87	37.5
88	35.0
89	26.0
90	16.5
91	17.0
92	20.0
93	18.0
94	16.0
95	13.5
96	12.5
97	12.5
98	14.0
99	17.5
100	79.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33927277512714676
2	0.10583188926487329
3	0.0997329606266051
4	0.10601594472446438
5	0.11630631814705679
6	0.12685604244452758
7	0.1395642353135665
8	0.13925468749516332
9	0.10942097072689944
10-14	0.10952805753975242
15-19	0.10841368539350096
20-24	0.1169990359676996
25-29	0.10726417538678208
30-34	0.13997752348192102
35-39	0.2780007126292749
40-44	0.14991317183693792
45-49	0.23216253703384088
50-54	0.3300733486104942
55-59	0.12516440544774043
60-64	0.0953039169762633
65-69	0.0963613992531866
70-74	0.09464131732173539
75-79	0.10063148591569976
80-84	0.10984429828395893
85-89	0.10533326629252654
90-94	0.11940179633108867
95-99	0.10904616688191394
100-101	0.11060059889973319
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.1952919E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	71.72419701548336
#Duplication Level	Relative count
1	100.0
2	42.99270072992701
3	21.615479961437817
4	12.882523068447872
5	9.073130422806775
6	7.025203140063352
7	5.749896708442363
8	4.94146811733921
9	4.050406280126705
10++	67.11334526924666
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCT	32602	0.2727534587994782	No Hit
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATA	28397	0.23757376754581874	No Hit
GATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGA	22851	0.19117505941435728	No Hit
CTCCTGTTCACTTTTCTTCCCCAATTCGAGGCTCTACACGCAAATGGCTG	18355	0.153560816399743	No Hit
GATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCT	15827	0.13241117086127666	No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAG	14383	0.12033043978629822	No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTT	13149	0.11000660173468924	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	1002190	3.1787612	10.152218	1
CCCCC	925895	2.7677915	13.668476	1
GGGGA	858020	1.7247527	5.2676635	2
CCCCA	890850	1.7078315	10.626501	1
GCAGG	851495	1.69147	6.1855135	4
GGCCC	550050	1.6837139	15.469249	9
CTGCA	1291395	1.6114213	5.4970655	2
GGGCC	504400	1.5623864	5.121137	1
CCCAA	1250485	1.5374031	5.1402106	2
CTGGG	771120	1.5363765	5.339148	1
CCTGG	779105	1.5339968	5.61754	1
GCCCA	779785	1.512734	5.8616977	1
TGGCC	759480	1.4953567	11.003849	8
GCCCC	490940	1.4850714	8.097348	1
CCCCT	764285	1.4695666	9.25777	1
CCTCC	757890	1.4572703	5.776303	1
CGGGG	455025	1.4262508	6.0725265	1
CCCAG	734285	1.4244668	6.2036214	1
CCCTG	724890	1.4104357	6.0898614	1
CCTGC	720010	1.4009407	5.107321	1
CTGGA	1107695	1.3986771	9.063715	2
ACTGG	1089910	1.3762201	7.7998915	1
CCAGT	1092560	1.3633121	5.66583	3
ATGGC	1069380	1.3502971	7.1796665	7
CTCCA	1071175	1.3208797	5.486859	1
ATGCA	1637680	1.3105328	6.427608	6
CTCCC	677575	1.3028408	8.354027	1
TGCAT	1622540	1.3022902	5.638433	7
CCCAC	676985	1.297835	5.0395827	1
CTCCT	988245	1.2222525	8.121521	1
TCCTG	975245	1.2205547	6.1444044	2
CCCGG	389545	1.1924047	6.0358024	1
CCTGT	942675	1.1797922	6.816849	3
CCCCG	383675	1.1605995	7.683153	1
GCATC	878845	1.0966356	7.452653	8
GATGC	867155	1.0949492	8.098734	5
CATCT	1368830	1.0857121	5.2243366	9
GGATG	842320	1.0762709	8.503775	4
CTCGG	496080	0.97674274	5.7991824	1
CCTCG	488015	0.94954234	5.233917	1
TGGAT	1155245	0.9382826	6.12935	3
CCCGC	307025	0.9287367	5.3228345	1
GATCC	726270	0.90625024	6.515623	1
>>END_MODULE