##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename BiGoRNA_CLCtrim_adp.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 47644404 Filtered Sequences 0 Sequence length 20-50 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.44143786539968 34.0 34.0 34.0 31.0 34.0 2 33.55187807575471 34.0 34.0 34.0 31.0 34.0 3 33.56890620354911 34.0 34.0 34.0 31.0 34.0 4 36.7098098446147 37.0 37.0 37.0 37.0 37.0 5 36.71478461562873 37.0 37.0 37.0 37.0 37.0 6 36.7199010402145 37.0 37.0 37.0 37.0 37.0 7 36.567207766939426 37.0 37.0 37.0 35.0 37.0 8 36.59657696631067 37.0 37.0 37.0 35.0 37.0 9 38.506476857177184 39.0 39.0 39.0 38.0 39.0 10 38.54617562221998 39.0 39.0 39.0 38.0 39.0 11 38.584018345575274 39.0 39.0 39.0 38.0 39.0 12 38.58546229269653 39.0 39.0 39.0 38.0 39.0 13 38.58500654137682 39.0 39.0 39.0 38.0 39.0 14 40.275885894175524 41.0 40.0 41.0 39.0 41.0 15 40.270142092657935 41.0 40.0 41.0 39.0 41.0 16 40.25503467311712 41.0 40.0 41.0 39.0 41.0 17 40.228826789395875 41.0 40.0 41.0 39.0 41.0 18 40.185830218381994 41.0 40.0 41.0 39.0 41.0 19 40.222351401436356 41.0 40.0 41.0 39.0 41.0 20 40.21601458169148 41.0 40.0 41.0 39.0 41.0 21 40.203367873581264 41.0 40.0 41.0 39.0 41.0 22 40.169768252401305 41.0 40.0 41.0 39.0 41.0 23 40.167203714048064 41.0 40.0 41.0 39.0 41.0 24 40.15528747286668 41.0 40.0 41.0 39.0 41.0 25 40.11960562165566 41.0 40.0 41.0 38.0 41.0 26 40.09283527293161 41.0 40.0 41.0 38.0 41.0 27 40.11531093456744 41.0 40.0 41.0 38.0 41.0 28 40.08742863554031 41.0 40.0 41.0 38.0 41.0 29 40.066708005230026 41.0 40.0 41.0 38.0 41.0 30 40.0415831953006 41.0 40.0 41.0 38.0 41.0 31 40.00459699291145 41.0 40.0 41.0 38.0 41.0 32 39.987128547158065 41.0 40.0 41.0 38.0 41.0 33 39.95386815377672 41.0 40.0 41.0 38.0 41.0 34 39.924773286377714 41.0 40.0 41.0 38.0 41.0 35 39.97523529355194 41.0 40.0 41.0 38.0 41.0 36 39.98142128540165 41.0 40.0 41.0 38.0 41.0 37 39.942615312969494 41.0 40.0 41.0 38.0 41.0 38 39.93698734969237 41.0 40.0 41.0 38.0 41.0 39 39.88629042519579 41.0 40.0 41.0 38.0 41.0 40 39.87562870804466 41.0 40.0 41.0 38.0 41.0 41 39.84893446345224 41.0 40.0 41.0 38.0 41.0 42 39.811344895533324 41.0 40.0 41.0 38.0 41.0 43 39.77353269741279 41.0 40.0 41.0 38.0 41.0 44 39.73697868409644 41.0 40.0 41.0 37.0 41.0 45 39.786073676575164 41.0 40.0 41.0 38.0 41.0 46 39.785946766998514 41.0 40.0 41.0 37.0 41.0 47 39.77663130420312 41.0 40.0 41.0 37.0 41.0 48 39.745920673117006 41.0 40.0 41.0 37.0 41.0 49 39.78941575013891 41.0 40.0 41.0 37.0 41.0 50 39.1778406337948 40.0 39.0 41.0 35.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 25.0 19 255.0 20 1056.0 21 3550.0 22 8031.0 23 13842.0 24 19385.0 25 25746.0 26 33094.0 27 44350.0 28 62470.0 29 86911.0 30 112282.0 31 151965.0 32 205055.0 33 279879.0 34 408895.0 35 660944.0 36 994315.0 37 1729928.0 38 4650487.0 39 3.7866683E7 40 285162.0 41 93.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.195398527617158 20.35301020254174 18.95361180102544 33.497979468815664 2 23.55253503034984 21.19768745120098 37.51410868853423 17.735668829914953 3 24.153014327958495 27.32594959059303 29.483970215094107 19.037065866354368 4 22.928699997116137 34.51058741381621 22.6301633505653 19.930549238502355 5 21.37093881459568 37.44133675977295 25.483575174267063 15.704149251364305 6 18.004136141744418 36.77714750202972 27.602136763097583 17.616579593128275 7 14.339535929940645 24.6547165111804 45.13635295782185 15.86939460105711 8 19.25909478458263 24.73247744530028 33.17161721062084 22.836810559496254 9 19.81405074766714 24.500266001897348 33.20161445216738 22.484068798268137 10 18.42740633584233 39.41387737937465 26.74311202876124 15.415604256021783 11 22.41895943275911 31.287560700497323 23.473142550561946 22.82033731618162 12 20.28459204178293 27.11094539246425 30.75032417281885 21.85413839293398 13 18.210980827835623 29.586866750680763 32.81204001663491 19.390112404848704 14 19.03292431338933 29.7585060330412 32.53653491978134 18.67203473378813 15 20.180671595324238 29.484424027984346 30.526052346274202 19.80885203041721 16 19.447772438899406 29.801743237500805 31.27450008224244 19.475984241357345 17 21.02644758105529 29.736971584750517 29.310162079309286 19.926418754884907 18 19.987112610405827 29.542775438401563 30.11914925172644 20.350962699466166 19 19.631247774659958 30.777312273651276 30.3534324828578 19.23800746883097 20 20.59052158869587 29.603981378765333 30.35148487251386 19.454012160024934 21 20.258666225981905 30.981743060899486 29.452430363093274 19.30716035002533 22 20.31293297653408 30.22783319690811 30.25662062581389 19.20261320074392 23 19.602669117440506 30.287526428126604 30.302449756274 19.807354698158893 24 20.13274007841113 30.58208517932822 30.135186974642004 19.149987767618644 25 20.749399339437186 29.27285504831241 30.340897655101134 19.636847957149268 26 20.04877599301855 30.03237485375404 30.668298904215202 19.250550249012214 27 19.876353215821993 29.004882215954524 30.504651800670707 20.614112767552772 28 19.828435807916833 29.5238466822913 30.962257814905204 19.685459694886664 29 20.40080590874221 30.763217719132342 29.14694528536283 19.68903108676262 30 20.021542448202894 31.354361208695785 29.210402520695112 19.413693822406206 31 20.58994029821942 31.316766438024306 28.7274657769629 19.36582748679337 32 20.77217933166425 30.364578503808847 28.634025967308418 20.229216197218488 33 20.98446355992271 29.46770903516272 29.07690603356649 20.470921371348076 34 20.95349802428464 29.50436665378845 29.08949283327561 20.452642488651296 35 20.16218817140207 30.077825953327153 29.97354454208741 19.78644133318336 36 20.541728823791193 30.365729233345657 28.99647304475475 20.096068898108403 37 19.69632656403179 30.210204612150708 29.86797275926269 20.22549606455481 38 20.553996801596174 30.307908423681333 29.740452399626765 19.39764237509572 39 20.123664973216197 30.41444170986302 29.45663833343494 20.00525498348584 40 19.730526479946764 30.324454553590336 29.542500670147938 20.402518296314966 41 20.20488275698273 29.638996068491934 30.21138369579248 19.944737478732854 42 20.978926348143013 31.10864456442835 28.157630381618198 19.754798705810437 43 20.56964313537471 30.62597358664575 28.592865763169932 20.211517514809604 44 20.659901618384286 29.39565863893349 29.874812539042427 20.069627203639794 45 20.567222194908723 29.062320047257188 30.2155870360438 20.154870721790285 46 21.034156797315525 30.012213594050046 29.275307528184918 19.678322080449508 47 20.82118157668298 30.331366156159522 29.51171773572509 19.335734531432404 48 20.48796988035916 29.220265457709754 29.522727571270384 20.769037090660703 49 21.12469888001441 29.37188101322952 29.89136937358248 19.61205073317359 50 20.291656827368527 30.132161127804984 29.46756918610084 20.108612858725653 >>END_MODULE >>Per base GC content fail #Base %GC 1 60.693377996432815 2 41.288203860264794 3 43.19008019431286 4 42.85924923561849 5 37.07508806595998 6 35.6207157348727 7 30.20893053099776 8 42.095905344078886 9 42.29811954593528 10 33.843010591864115 11 45.23929674894073 12 42.138730434716905 13 37.60109323268433 14 37.70495904717746 15 39.98952362574145 16 38.92375668025675 17 40.9528663359402 18 40.338075309871996 19 38.869255243490926 20 40.04453374872081 21 39.56582657600724 22 39.515546177278 23 39.4100238155994 24 39.28272784602977 25 40.386247296586454 26 39.29932624203076 27 40.49046598337477 28 39.5138955028035 29 40.08983699550483 30 39.435236270609096 31 39.95576778501279 32 41.00139552888274 33 41.455384931270785 34 41.40614051293594 35 39.94862950458543 36 40.637797721899595 37 39.921822628586604 38 39.9516391766919 39 40.12891995670204 40 40.13304477626173 41 40.14962023571559 42 40.73372505395345 43 40.78116065018432 44 40.72952882202407 45 40.72209291669901 46 40.71247887776504 47 40.15691610811539 48 41.25700697101986 49 40.736749613188 50 40.40026968609418 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 89434.0 1 59899.0 2 23166.5 3 12220.0 4 8193.5 5 7610.5 6 7157.0 7 7600.5 8 8279.5 9 11331.0 10 14489.5 11 23143.5 12 32445.0 13 52013.5 14 70370.0 15 99663.0 16 130229.5 17 222008.5 18 313933.5 19 464993.0 20 616278.0 21 639068.5 22 661306.5 23 942167.0 24 1223447.5 25 1524107.5 26 1825092.0 27 2015835.5 28 2206845.5 29 2608651.0 30 3009648.0 31 2963133.0 32 2911368.0 33 2888005.5 34 2870430.0 35 2721743.0 36 2573612.0 37 2794180.5 38 3016464.0 39 3066624.5 40 3139932.0 41 3152744.5 42 3126482.0 43 3183551.0 44 3254669.5 45 3254212.0 46 3251288.0 47 3077151.5 48 2904457.0 49 2777555.5 50 2646107.0 51 2381059.5 52 2118307.5 53 1897611.5 54 1674635.0 55 1466862.0 56 1264322.5 57 1120415.0 58 972037.0 59 841577.0 60 711560.0 61 592641.0 62 471639.0 63 383799.5 64 296848.0 65 239294.0 66 182323.5 67 144499.5 68 108182.0 69 87409.5 70 67874.0 71 57360.0 72 47000.0 73 37149.5 74 27044.5 75 21900.0 76 16833.5 77 12558.0 78 8425.0 79 5497.0 80 2712.0 81 1736.5 82 821.5 83 609.5 84 399.5 85 299.0 86 228.5 87 204.0 88 162.0 89 117.0 90 94.5 91 82.0 92 71.5 93 61.0 94 49.0 95 46.0 96 45.5 97 43.0 98 24.5 99 8.5 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.197764757430904E-6 2 8.395529514861808E-6 3 6.296647136146356E-6 4 4.197764757430904E-6 5 4.197764757430904E-6 6 4.197764757430904E-6 7 6.296647136146356E-6 8 2.098882378715452E-5 9 1.4692176651008165E-5 10 1.049441189357726E-5 11 5.24720594678863E-5 12 1.3432847223778893E-4 13 3.777988281687814E-5 14 4.008865343346514E-4 15 1.2173517796549622E-4 16 0.010007471181715275 17 4.155787109856595E-4 18 0.031269149678102806 19 0.0 20 0.010553180600181292 21 6.506994809517172E-5 22 0.003134127056083388 23 1.2597053473610204E-4 24 0.001081384992543273 25 7.413174142873789E-4 26 2.1003674004664244E-6 27 5.818905249232324E-4 28 2.2481131082432846E-4 29 1.050695490017794E-5 30 1.450229053170042E-4 31 0.0014232176196443554 32 3.5744924220760654E-4 33 6.373016304026616E-4 34 0.00561720419629137 35 3.956255347915515E-4 36 0.0031658635708562474 37 6.949463208720474E-4 38 2.1071519201537767E-6 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 20 3364.0 21 4171.0 22 6683.0 23 6074.0 24 6183.0 25 7211.0 26 7262.0 27 7986.0 28 7943.0 29 8836.0 30 10421.0 31 9070.0 32 14994.0 33 11663.0 34 12860.0 35 12898.0 36 21103.0 37 28259.0 38 20547.0 39 36894.0 40 57589.0 41 25677.0 42 60393.0 43 134511.0 44 34797.0 45 99208.0 46 560706.0 47 65592.0 48 83860.0 49 98034.0 50 4.6179615E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Duplicate Percentage 83.79377190674494 #Duplication Level Relative count 1 100.0 2 37.6073001418228 3 20.90206762708069 4 14.163618720609092 5 11.336493244756289 6 9.074792864074047 7 7.755467642009405 8 6.585429573785175 9 5.758751959393894 10++ 160.0339628274987 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGACTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGT 298887 0.6273286575271254 No Hit GGACTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTAT 158528 0.3327316257330032 No Hit ATAATAATCAATCAAAGAATATCTTAGACAGGTGAACGATTTGCTAAGCC 151006 0.31694383248030555 No Hit GGGACTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTA 141750 0.2975165771829153 No Hit GGCCATTATACTAAAGGTACTTTTTTTTAACGCTTAAAAACAATGCTTTC 134377 0.28204151740464634 No Hit GGGAGGTGCTAATACAGCAAAATCTCTTGAAAGGCCATTATACTAAAGGT 127867 0.26837779311920873 No Hit CAGCATAATAATCAATCAAAGAATATCTTAGACAGGTGAACGATTTGCTA 124740 0.26181458792096546 No Hit CCCACATTAAAGCTGCACTTAAAAGCATTTTTCGAACAACCAGCTATCTT 105727 0.22190853725444862 No Hit GTCCTTTCGTACATGCCGAGATAAGTTTATGTAAAGCAGATAGAAACCAA 97328 0.20428002415561752 No Hit CCACATTAAAGCTGCACTTAAAAGCATTTTTCGAACAACCAGCTATCTTA 90185 0.18928770732445305 No Hit ATTAAATTATCATTCTGCAATTTTTTTCTGGATTTACTCTTTCCAGTCTC 88279 0.1852872375106214 No Hit CTAGTGAGTATCTTTAACACACTACGCCCCACATTAAAGCTGCACTTAAA 82161 0.17244627511764024 No Hit ATTTTTTAGAAATTATTCTTTTACTATTAGAAAGACAAGTTGTTAAATAC 79981 0.16787071153204058 No Hit GAACGATTTGCTAAGCCTCATTTTTTAGAAATTATTCTTTTACTATTAGA 76754 0.16109761809592582 No Hit CTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGG 67752 0.1422034789227293 No Hit CCACAATCAAAAGGCAAGGAATTTCGCTACCTTAGCACCCTCACGCTAGG 67638 0.14196420633155574 No Hit AATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGGAGCATTAG 58891 0.12360528216493168 No Hit GTCAAATCCGGAAAGAGTTAGTTATTCAGAAAGGTTAGACTTGCTTTCTA 57640 0.12097958030915866 No Hit CTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGGAGC 56668 0.11893946663704723 No Hit CCAGCATAATAATCAATCAAAGAATATCTTAGACAGGTGAACGATTTGCT 55559 0.1166118060790518 No Hit CTTAAAACTCGAAATTGCATATCACATCCTTAGCAAAGTTTATTCAAGAA 55301 0.11607029442534322 No Hit CTCATTTTTTAGAAATTATTCTTTTACTATTAGAAAGACAAGTTGTTAAA 55049 0.11554137606590692 No Hit CTTTCCTAAAAATCTTTTTATTTCGGGAGGTGCTAATACAGCAAAATCTC 53992 0.11332285739160469 No Hit CCTGATTCAACATCGAGGTGCCAATCCCTTTAGCCAATACGAACTCTACT 51868 0.10886483121921306 No Hit ATTATACTAAAGGTACTTTTTTTTAACGCTTAAAAACAATGCTTTCATCT 51373 0.10782588444174891 No Hit GGAGGTGCTAATACAGCAAAATCTCTTGAAAGGCCATTATACTAAAGGTA 51236 0.10753833755586491 No Hit CTGCTTTCCTAAAAATCTTTTTATTTCGGGAGGTGCTAATACAGCAAAAT 50042 0.10503227199567865 No Hit TAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGGAGCA 49839 0.10460619887279943 No Hit CTAAAAATCTTTTTATTTCGGGAGGTGCTAATACAGCAAAATCTCTTGAA 48739 0.10229742825621241 No Hit >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position TTTTT 19697400 3.836273 7.0163846 21 AAAAA 13733955 2.6724303 5.2335596 29 GGAGG 2855900 2.515796 8.472564 2 AGCAG 3983310 2.4660861 10.517376 21 CAGCA 3698720 2.3474848 6.277476 15 CCAGC 2454390 2.3293648 7.245107 40 GCTGC 2376265 2.2002883 7.2592516 12 CTTTT 7334885 2.1357899 5.495195 34 TTTCT 7334755 2.1357522 5.864383 37 CTTTC 4886025 2.1270883 5.8217964 4 GAAAG 4901265 2.029223 5.2090406 29 GCCCC 1404290 1.992936 6.892042 26 TGAAG 4789945 1.9834903 7.493846 45 TTTTC 6762960 1.9692553 5.561094 36 AGGTG 3242575 1.9585963 6.3233004 34 CCCCA 1964800 1.911611 5.213938 27 CAGCT 3011055 1.9113842 5.3195834 40 CTGCA 2983610 1.8939625 5.658643 13 GGCCA 2045085 1.8932945 9.387344 1 AGCTG 3010410 1.8640939 5.8756576 10 GGTGC 2006065 1.8119347 7.379024 5 CCCAC 1858490 1.8081787 9.684802 1 AGCAA 4258965 1.8076438 7.221312 11 GGGAG 2050470 1.8062829 8.810586 1 CTGGT 2899025 1.7954447 5.775868 1 AAAGG 4336325 1.7953264 5.117604 30 TAGCA 4166610 1.7687627 7.0304546 10 CCACA 2713610 1.765574 6.8326383 1 ACCAG 2702195 1.7150155 5.346091 39 GGGGA 1943920 1.7124218 15.695891 1 AAAAC 5851265 1.7025626 5.9843135 30 GCCTC 1788725 1.6979132 5.989176 15 GACAG 2664780 1.649778 6.200858 31 GCAGT 2650940 1.6415045 9.683783 22 CGCCC 1143015 1.6221405 6.378416 25 TTGCT 3807835 1.6170402 5.24353 41 ACAGC 2547100 1.6165806 5.137798 14 ACAGG 2600375 1.6099046 6.240147 32 AAATG 5569060 1.5809788 5.2632213 42 CTAAA 5426965 1.5793861 5.1007695 40 GAAGT 3693895 1.529622 7.049242 46 ACTTT 5249375 1.5282516 5.233288 33 AGGCC 1639025 1.5173728 7.2658896 32 CAGTT 3543270 1.5044194 7.1093173 23 ATGAA 5287190 1.5009596 5.3548965 44 GGTGA 2432305 1.4691732 6.1716275 35 CAGGT 2357570 1.4598451 6.1311235 33 GGGAC 1615100 1.458542 14.824455 2 ATCAA 4977935 1.448707 5.524612 11 GCCAT 2212715 1.4046069 5.6910176 2 GTGCT 2264785 1.4026427 5.371043 6 GCAAT 3282945 1.3936391 6.957583 12 AGTTT 4819240 1.3686066 6.238807 24 AAGGC 2170595 1.3438257 5.707393 31 AACTT 4607820 1.3412348 5.11817 32 AAACT 4559050 1.3268007 5.17426 31 GTTTA 4644945 1.3191088 6.102317 25 GAGGT 2151740 1.2997049 5.487677 3 AATCA 4398195 1.2799877 5.636413 10 AATGA 4337680 1.231407 5.190075 43 GGACT 1969475 1.2195302 10.874129 3 AAGCC 1796465 1.1401713 5.6111045 46 TCTAA 3825060 1.1133906 5.0956674 39 TTCTA 3781085 1.100788 5.0003834 38 CTAGC 1708055 1.0842544 9.569437 9 ATAGC 2512980 1.0667822 7.1181803 19 ACTAC 2110710 0.91854936 7.107994 5 CTACT 2075180 0.90324926 6.8711977 6 GAACG 1433975 0.8877808 5.3918023 38 ACTAG 1989560 0.8445858 6.419583 8 GACTA 1494690 0.6345091 6.8563495 4 >>END_MODULE