FastQCFastQC Report
Mon 3 Feb 2014
filtered_BS_CgLarv_T1D3_TGACCA_L007_R2.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename filtered_BS_CgLarv_T1D3_TGACCA_L007_R2.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 3343987
Filtered Sequences 0
Sequence length 76
%GC 17

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 38840 1.5202572E7 2.57814672E8 40
CGGGG 13820 209692.97 6460050.5 39
GGGCG 13025 197630.31 4833701.0 43
GGGGC 12175 184733.14 3701521.8 41
GGCGG 7730 117288.47 3530536.8 12
GAGGG 20255 111790.49 3005279.2 27
GGGGA 19225 106105.734 2097905.8 20
AGGGG 18290 100945.35 1348497.2 27
GGTGG 13645 90876.14 4513970.0 40
GCGGG 5560 84362.73 3514114.8 13
GGGAG 15015 82870.11 1297936.8 30
GTGGG 6360 42357.805 955157.9 41
GGAGG 6795 37502.66 944188.7 18
GGGGT 5095 33932.863 981556.7 41
TGGGG 4360 29037.74 746043.0 19
GGGTG 3500 23310.111 563726.9 16
GGCGC 7745 4555.491 175462.45 44
GAGCG 18525 3963.4077 128157.24 9
TCGGG 14525 3749.9932 87385.055 38
GGAAG 41755 3249.494 77639.52 5
GGCCG 5250 3087.9702 98302.414 12
CGGTG 10785 2784.418 167349.73 39
GGGCC 4670 2746.823 94281.06 11
GCCGG 4655 2738.0002 106775.96 13
GGTCG 10300 2659.2031 143466.45 43
CCGGG 4360 2564.4858 108042.56 14
GCGGC 4250 2499.7854 50848.215 11
CGGCG 3875 2279.216 48939.348 12
GGGAA 28560 2222.6208 97986.47 21
CGGGT 5415 1398.018 73928.94 15
AGGGA 17880 1391.4729 65976.625 20
TAGGG 13410 1259.3307 63591.05 19
GTAGG 13105 1230.6881 61691.94 18
CGGGA 5685 1216.3008 31830.578 3
AGAGG 15610 1214.815 41995.508 26
GGGTA 11160 1048.0336 38249.844 29
GGGTC 3955 1021.0824 32656.76 42
AAGGG 12985 1010.52997 12339.788 27
GGTAG 10685 1003.4264 36052.555 30
GCGCG 1660 976.3868 11271.59 45
GTCGG 3705 956.5387 48634.582 13
GGAGA 11350 883.28955 19030.32 31
GTGGT 7720 874.8469 52758.58 41
TGGGC 3270 844.2325 34238.426 42
AGGGT 7930 744.70496 35325.168 28
GGCGT 2555 659.63727 14227.705 13
AGGGC 3075 657.89355 29590.967 10
AGCGG 2965 634.3592 17954.936 10
CGCGG 1055 620.535 18076.26 46
GAGTG 6570 616.98755 23181.92 27
CGTGG 2240 578.3122 17482.568 15
GAGAG 7185 559.1573 7058.1562 6
GCGTG 1970 508.60486 12553.33 14
CGCCG 19020 433.67288 20123.123 46
GTGGC 1615 416.95273 17769.02 41
GAAGG 4905 381.72116 9802.994 6
CTGGG 1240 320.13712 10060.817 37
GCGGA 1400 299.52878 6859.374 3
CGGGC 485 285.26962 4451.279 42
GGCAG 1315 281.3431 3067.8374 71
GACGG 1185 253.52972 7701.7573 9
GATCG 62620 227.96178 3882.7273 1
GTGGA 2420 227.26175 5750.22 16
GCGGT 865 223.32143 4013.8992 47
GGACG 1005 215.01888 7864.342 43
ATCGG 57650 209.869 3931.4858 2
TGGAG 2210 207.5407 5885.4756 17
ACGGG 970 207.53065 5628.004 2
GCGCC 8710 198.59575 6471.6787 45
AGGCG 910 194.69371 2926.6677 9
TGGGA 2065 193.92377 6224.619 3
GGTGC 725 187.17693 3721.568 43
TTGGG 1645 186.41492 2775.5615 19
GTGTG 1585 179.61559 2924.9087 72
TGGCG 685 176.84991 4465.882 42
GGGCA 810 173.2988 2311.9712 11
TCGGA 47310 172.22728 3989.258 3
CGGAG 770 164.74083 3476.717 39
CGTCG 16415 164.28319 4978.2686 12
CTCGG 15615 156.2767 8832.911 37
TGTGG 1270 143.91911 1959.1934 17
GCGTC 13900 139.11278 5201.9873 11
GCCGT 13895 139.06274 7667.731 47
GCGAG 635 135.8577 1155.9075 17
CGGAA 44775 135.0768 3608.4634 4
AGGAG 1690 131.52065 1569.464 29
GCAGG 610 130.50897 1464.9132 27
GGGCT 505 130.3784 2046.8625 42
GTGCG 495 127.79665 740.405 72
GAGGA 1640 127.62951 1485.43 31
GGGAC 595 127.299736 2081.7375 42
CAGGG 590 126.22999 2003.6002 19
CGAGG 570 121.951004 2696.9392 18
ATGGG 1265 118.79592 4433.057 2
GAGGC 545 116.60228 1464.8053 41
GTTGG 1015 115.02197 2163.133 18
GATGG 1220 114.56999 4297.451 1
TGGTG 990 112.18892 2005.1975 39
GGTGT 930 105.38959 1510.8108 40
GGCTG 395 101.97915 2232.941 43
GTCGC 9685 96.92859 5576.624 44
GTGAG 990 92.97072 1082.253 30
AGTGG 985 92.50118 2571.564 28
TGGGT 815 92.357544 2085.4746 38
GGCGA 415 88.78889 462.75787 23
TGCGG 340 87.779526 1116.3212 40
GGAGC 410 87.71915 541.5331 44
GCTGG 325 83.9069 1304.2605 36
GAGGT 890 83.57974 879.3332 31
GCCGC 3650 83.22324 2127.1 44
GGTTG 705 79.89211 1102.0463 17
GGAGT 825 77.47561 1961.5895 31
GGGTT 670 75.925835 817.045 49
AGGTG 780 73.249664 746.07074 39
CCGCG 3170 72.278824 469.91806 45
AGAGC 23655 71.36218 1985.8643 8
GGTGA 745 69.962814 642.5703 29
CGCGC 2915 66.464584 239.07028 46
GGGAT 675 63.389126 574.93134 29
CGCGA 6885 57.10214 95.140076 72
TCGCG 5600 56.045437 144.7464 45
GTCGT 12250 53.813206 1677.2113 13
AAGAG 47700 52.343197 939.5667 7
CGTGT 11415 50.145123 1884.5062 15
GCCCG 2130 48.56589 2019.7317 46
AGCGT 13200 48.053265 1847.4698 10
TCGTG 10835 47.597225 1745.1755 14
TCGGT 10715 47.07008 2756.591 38
TGAGG 495 46.48536 676.49146 17
CGACG 5510 45.6983 164.30226 12
CGCGT 4500 45.03651 202.63574 46
ACGCG 5085 42.17348 152.93756 45
GAAGA 38280 42.00624 979.0433 6
GGATG 440 41.320324 507.36862 17
GGAAA 30810 33.809097 1256.3157 22
GTCCG 3365 33.677303 111.79185 1
TGGTC 7155 31.431301 1985.1895 42
GGCCC 1300 29.641157 667.7783 45
CGGCC 1255 28.615118 981.057 45
GTGTA 16370 26.157614 665.32196 16
GCCGA 2980 24.715233 704.69305 47
TGTAG 14525 23.209486 707.3308 17
CCCGG 915 20.862814 699.3038 37
GAAAG 18480 20.278875 889.2631 23
CCGGA 2255 18.702301 461.79892 47
TCCGG 1785 17.864483 830.5681 37
GACGC 2125 17.62412 350.8737 44
GACCG 2110 17.499714 274.93658 1
AGCGC 2105 17.458246 770.70044 10
CCGTG 1665 16.66351 731.744 14
GTAGA 12015 15.909971 843.61176 31
TCGGC 1515 15.162293 233.19942 71
GCTCG 1400 14.011359 90.28471 36
GCGAC 1545 12.813772 98.58552 11
GCGAA 4000 12.0671625 22.815004 29
GCGCA 1440 11.942933 74.967354 47
AAAGG 10865 11.922618 179.19003 26
CGGCA 1430 11.859996 190.28018 72
CCGGC 505 11.514449 362.78864 48
GCGCT 1130 11.30917 54.278427 47
TGGCC 1130 11.309169 486.8977 42
GAACG 3730 11.252629 100.00673 1
CCGGT 1100 11.008926 264.19485 48
GCACG 1295 10.740344 47.570038 72
TTCGG 2340 10.27942 385.17154 37
AGTGT 6410 10.242536 396.10526 28
GCGTA 2790 10.156712 100.04876 48
AGAGT 7530 9.971045 332.22226 26
GACGA 3150 9.502891 25.955921 60
GAGAT 7085 9.381786 292.8033 32
CCCCC 267105 9.151868 11.328664 4
AAGCG 3025 9.125792 291.04446 9
GTACG 2370 8.627745 31.48157 1
GTCGA 2315 8.427524 26.101109 60
GCGTT 1915 8.41243 56.96471 11
GCGAT 2265 8.245503 18.270012 71
GTTCG 1865 8.192785 117.13338 1
GGTCC 800 8.006491 277.712 43
GAGCA 2645 7.9794116 192.4998 72
CCCCG 8750 7.7338867 50.492012 46
AGAGA 6860 7.527764 88.50942 72
GGCTC 745 7.4560447 220.05986 50
CGGAT 2000 7.280798 293.56415 48
GACGT 1990 7.244394 40.64806 13
ACGGC 870 7.215522 367.42694 10
ACCGG 865 7.1740522 236.10031 2
TCGCC 16690 6.475101 215.74565 45
CCGCC 7255 6.412497 78.61771 45
GGATC 1745 6.352496 190.29097 49
CTCTC 952995 6.290994 8.2353 70
CGCCC 6915 6.1119795 71.58684 45
CGGTC 605 6.0549088 122.62608 42
CGGCT 585 5.8547473 180.39459 49
CCCGC 6365 5.62585 21.731134 47
AGAAG 5030 5.5196285 88.90531 68
TCTCG 30690 5.2262187 146.00209 36
GTGCC 520 5.2042193 141.13454 44
CCGTC 12760 4.950407 19.921764 48
TCTGG 1085 4.766312 141.07976 36
TGCCG 470 4.7038136 188.16179 46
CAGAG 1555 4.6911097 67.04999 71
AGACG 1535 4.6307735 115.091095 8
GGCCT 460 4.6037326 169.42697 12
AACGG 1520 4.5855217 101.09954 2
CGTGC 455 4.5536923 65.143936 48
CTGCG 455 4.553692 32.52678 39
CCCGA 13930 4.478552 24.527462 47
GCCTG 435 4.3535295 115.35365 13
TGCGC 430 4.303489 28.950678 44
CGAGA 1415 4.268759 38.932537 72
CAGCG 500 4.1468515 26.75815 72
GCAGA 1355 4.0877514 55.15443 72
CCGTA 28725 4.0536566 107.52283 48
GCTGC 395 3.9532053 72.37669 44
CGCTG 395 3.9532049 25.233803 12
GAGAA 3550 3.895563 75.086975 31
AGATC 74630 3.830867 43.86168 33
CGCAG 460 3.815103 38.982315 46
GTGTC 865 3.799871 31.659098 41
CGTCC 9455 3.6681895 5.733672 50
CGGTT 830 3.6461186 83.93243 49
CTCGC 9350 3.6274533 7.9273887 72
CGGAC 435 3.607761 71.73175 42
AGCCG 435 3.607761 62.971443 46
AGGAA 3285 3.6047676 68.0035 20
CTGTG 820 3.6021898 58.65087 38
CTCCG 9175 3.5595593 24.499104 36
CCTCG 8980 3.4839065 7.139739 36
CCGAC 10755 3.4577763 5.580549 48
CCTGG 345 3.4527993 79.30231 14
TCTCT 1186090 3.436741 5.0512457 72
CGGTA 935 3.4037726 32.910778 48
ACGGA 1125 3.3938897 113.021866 3
CCGAT 23965 3.381928 17.962997 48
CGACC 10510 3.3790073 4.998623 45
TCCCG 8640 3.3519993 11.75957 36
CGTGA 920 3.349167 112.766594 15
TCCGT 19620 3.3411016 6.8203845 37
TGTCG 760 3.338615 37.994057 43
CGCTC 8460 3.2821662 10.4894495 49
GAGCC 395 3.276013 26.88696 11
CCGAA 27935 3.26687 8.711634 48
ACCGA 27935 3.2668695 4.444792 57
GCAGC 390 3.2345443 47.982727 44
CCGCT 8330 3.2317314 16.975588 48
TACCG 22875 3.228108 4.9391685 37
TCCGC 8130 3.1541386 5.4252195 57
ACGAG 1035 3.1223783 32.59776 17
CACGG 370 3.0686698 83.791824 37
CCCGT 7885 3.059088 26.651863 47
ACCCG 9470 3.0446432 5.8120894 46
CGAAA 70395 2.9944816 5.4389677 4
CGAGC 350 2.9027963 20.811737 71
TTCCG 16950 2.8864257 8.111228 36
TCGAG 790 2.8759153 18.27042 69
AGCGA 945 2.8508673 38.030376 10
CGCCT 7335 2.8457077 11.922984 46
ACCGC 8805 2.830843 5.301629 72
CCGAG 340 2.8198593 80.65348 14
AAGGA 2535 2.7817612 56.933167 20
CGTTG 630 2.767536 45.88631 12
GGCAC 330 2.7369223 29.888227 43
AGCAG 875 2.6396918 38.048214 2
ACGTG 725 2.6392891 44.57987 14
GGTTC 600 2.6357486 91.84161 50
ACGCC 8190 2.6331184 12.903423 45
CACCG 8125 2.6122203 5.347122 46
GTGAC 705 2.5664814 34.087105 30
GACAG 845 2.5491881 19.466125 71
GAGAC 845 2.5491881 20.547577 71
TAGAG 1915 2.5357966 39.109894 19
TTTCG 33435 2.499151 7.309412 36
CGTAT 40115 2.4848158 48.67939 49
CTTGG 565 2.4819965 71.328064 37
CCGCA 7675 2.4675436 14.067633 48
TGCGT 560 2.4600322 23.621586 72
GAGCT 670 2.4390674 31.46039 33
AAGGC 805 2.4285166 54.28825 8
CGTCT 14070 2.395989 8.717265 49
CTTCG 14055 2.3934345 5.5303826 36
CCGTT 13865 2.3610792 11.577045 48
CAGTG 645 2.3480573 44.59648 28
CGTCA 16575 2.3390553 6.811208 12
AAGTG 1765 2.3371704 74.9192 27
GCCCC 2640 2.3334239 22.691568 44
GTGCT 530 2.3282444 45.92529 49
GGTCT 525 2.30628 36.41097 43
CACGC 7170 2.305184 8.067697 72
CGATC 16075 2.2684953 8.648425 49
ACTCG 15450 2.1802957 6.619972 35
CTCGT 12800 2.1797197 5.433076 72
AGATG 1645 2.1782694 64.083244 72
CGTTT 29090 2.1743772 5.146663 49
CCACG 6760 2.1733673 5.532093 71
ACACG 18240 2.1330843 8.677914 71
ATCGA 41455 2.127946 7.0293 72
CACGA 18090 2.1155422 7.336455 72
GGACC 255 2.1148944 26.899406 43
GGTCA 580 2.1114311 26.244463 50
GTCTG 480 2.108599 20.56957 13
CGCTT 12345 2.1022375 5.463638 49
CGTAG 575 2.0932293 27.535284 17
TGTGT 1080 2.0824616 14.515686 72
GGCCA 250 2.0734258 35.84777 12
TGTGC 470 2.0646698 17.474092 48
GTGCA 565 2.0568252 15.660126 72
CAGGA 665 2.0061657 17.38798 3
TGGAA 1485 1.966401 55.323784 4
AGTCG 540 1.9658154 20.979801 12
GCTGT 445 1.9548471 14.17344 60
CTGGC 195 1.9515823 10.812538 34
GCAGT 530 1.9294114 19.67832 27
GTAAG 1455 1.9266757 55.798702 18
AGTGA 1445 1.9134344 37.21457 28
CATCG 13450 1.8980569 10.013493 12
TGAAG 1430 1.8935713 42.924915 17
TGGAC 520 1.8930074 82.64969 42
CGATT 30465 1.8870723 5.292208 49
GGCAT 515 1.8748055 47.20455 12
ACGGT 515 1.8748055 26.272352 38
ATGCG 515 1.8748052 14.355008 71
GTGAA 1405 1.860467 37.201874 16
TCAGG 510 1.8566033 13.111173 18
GGTAA 1400 1.8538461 36.24324 30
GTTGT 950 1.8317949 26.267384 60
GGAAC 600 1.8100744 18.46954 32
ATTGG 1105 1.765679 34.54791 2
AGGTC 485 1.7655935 22.287422 32
TAGGA 1310 1.7346703 50.556694 19
GAAGC 575 1.7346547 24.87394 69
AGTGC 475 1.7291896 20.973595 33
CTGGA 475 1.7291895 28.842026 34
ACTGG 475 1.7291895 32.77124 33
GTCAG 470 1.7109874 19.68403 26
ATAGG 1285 1.701566 51.029587 18
TAAGG 1275 1.6883242 54.3723 19
AGGAC 550 1.6592349 26.074644 32
CCAGG 200 1.6587404 20.91016 14
GAGTC 455 1.6563815 15.735512 11
GAGTA 1245 1.6485989 47.68719 29
TTGCG 375 1.6473429 18.897127 71
AGTAG 1240 1.6419781 32.42817 30
TGCAG 450 1.6381795 15.660736 59
CACGT 11570 1.6327521 5.7670913 72
CGAAG 540 1.629067 10.814514 71
TGTTG 840 1.6196923 26.267502 59
GCCAG 195 1.6172719 14.936458 13
CGCAT 11325 1.5981778 6.816994 49
TGCGA 435 1.5835736 13.112139 16
AGGCC 190 1.5758036 17.924639 11
TTGGA 985 1.573931 45.47372 3
TACGG 420 1.5289676 23.612528 2
GTTGC 345 1.5155555 44.47615 44
GCATG 415 1.5107653 18.357185 13
CAGGC 180 1.4928666 20.92176 42
TAGCG 410 1.4925634 19.57501 71
TTTGG 770 1.484718 28.527191 36
CTGGT 335 1.4716264 23.777382 38
TGAGA 1110 1.469835 17.088863 72
TTGTG 755 1.4557949 15.278713 14
GACTG 390 1.4197556 35.39767 32
TGCTG 320 1.4057326 12.681441 35
GGACA 465 1.4028077 8.697349 43
GGACT 380 1.3833516 34.08209 33
TGACG 380 1.3833514 14.355673 59
CGAGT 375 1.3651496 23.60231 15
AGGCA 450 1.3575559 25.012697 25
CTGAG 370 1.3469477 10.440241 70
GTGTT 695 1.3401026 13.890178 16
GATCT 20840 1.2908777 52.51072 34
ATGTG 805 1.286309 12.68522 35
GGCAA 425 1.2821361 27.187017 22
GTAGC 350 1.2741396 19.744978 44
AGCTG 350 1.2741396 10.509084 35
ACAGG 420 1.267052 14.150888 37
GGTAC 345 1.2559377 14.430898 43
CGATG 345 1.2559375 10.440489 59
GATGT 750 1.1984247 14.893707 60
GGATT 740 1.1824456 42.626766 49
GCAAG 390 1.1765484 13.0502405 23
TGAGC 320 1.1649277 9.135006 71
TTGGT 595 1.147282 23.65659 38
GATGC 315 1.1467258 14.355611 60
GAGTT 715 1.1424981 27.046385 32
TGTGA 715 1.1424981 9.208788 15
GATTG 705 1.1265192 36.273228 1
GGTTT 575 1.1087179 13.207117 49
CAAGG 365 1.1011286 13.038242 18
CAGGT 295 1.0739177 10.508942 38
GAAGT 795 1.0527198 18.121677 31
ATGAG 795 1.0527197 10.443351 70
GTAGT 655 1.0466242 13.235684 31
CATGG 280 1.0193117 11.821882 37
GCTGA 280 1.0193117 6.525277 60
AAGGT 765 1.0129945 14.31577 27
ATGGA 765 1.0129944 13.833358 3
GTATC 16255 1.0068723 43.918808 50
GATAG 750 0.99313194 13.359798 1
TGAGT 620 0.9906977 14.962078 31
TTGGC 225 0.9884057 9.498514 42
CTAGG 270 0.9829076 26.270847 37
TTAGG 600 0.9587397 32.803257 18
GGAAT 715 0.94678575 8.107566 5
GGCTT 215 0.9444765 17.419937 49
GCCCT 2405 0.9330507 11.081562 47
GAAAA 57175 0.8846729 6.39782 23
AATGG 665 0.88057697 12.406236 2
TGGTT 455 0.87733334 15.9820595 42
GTTTG 450 0.8676923 18.064367 1
TAGGC 235 0.8554937 13.1232395 24
GTGAT 535 0.8548762 8.63307 16
GGATA 645 0.85409343 7.153272 30
TGGCT 190 0.8346537 17.41394 42
GGTAT 515 0.82291824 13.248859 49
TGGCA 225 0.81908965 9.183298 42
GCTTG 185 0.8126891 6.3290987 13
TAGTG 505 0.80693924 5.728109 71
TAGAT 35660 0.80346215 14.070032 32
GTCCC 2070 0.80308324 9.398996 44
GGTTA 495 0.79096025 10.359684 16
TTGAG 490 0.7829707 6.8737307 71
AGGCT 210 0.7644838 6.559499 42
GAATG 575 0.7614011 12.882663 1
AGTTG 470 0.7510128 9.781428 33
GTTGA 470 0.75101274 11.456697 60
AGGTA 560 0.74153847 12.881966 28
TGATG 460 0.73503375 10.311074 59
GATGA 555 0.7349177 6.1995215 31
GATCA 14180 0.7278801 8.372721 70
GTTAG 450 0.71905464 12.661739 17
AGGAT 525 0.69519234 10.014445 32
TATGG 430 0.6870968 10.358923 18
AAAGA 43950 0.68004143 11.730266 24
ATGGC 185 0.67347383 6.5550056 34
GTCTC 3810 0.6488072 6.0155525 50
TGGTA 405 0.64714926 8.061747 42
TCAGA 11940 0.61289763 11.629626 72
AGGTT 380 0.6072018 5.179756 10
TGGAT 375 0.5992123 6.9100685 42
GCATC 3880 0.54754347 8.844775 11
ATCAG 9355 0.48020583 10.396662 71
ACAGA 11225 0.4774921 6.0844007 72
CACAG 3920 0.458426 5.911042 71
TAGGT 260 0.41545385 8.072304 38
TTTGT 5220 0.17126243 5.4925447 72
Produced by FastQC (version 0.10.1)