FastQCFastQC Report
Mon 3 Feb 2014
filtered_BS_CgLarv_T1D5_ACAGTG_L007_R2.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename filtered_BS_CgLarv_T1D5_ACAGTG_L007_R2.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 2709822
Filtered Sequences 0
Sequence length 76
%GC 16

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 33180 2.8993496E7 4.73523584E8 40
CGGGG 11365 348770.56 9674350.0 39
GGGCG 11280 346162.1 8448619.0 42
GGGGC 10615 325754.5 7493497.0 10
GGCGG 7175 220187.31 7991267.0 12
GGGGA 16750 179576.78 4480569.0 20
AGGGG 16425 176092.47 2990214.5 27
GCGGG 5100 156509.45 7737050.0 13
GAGGG 14380 154168.02 3318597.0 27
GGTGG 10195 136621.16 6165826.0 40
GGGAG 11200 120075.23 2710300.5 29
GTGGG 4955 66400.96 1545077.6 40
GGAGG 4900 52532.91 1938329.2 17
GGGGT 3795 50856.04 1366448.4 41
TGGGG 3335 44691.668 1206615.2 19
GGGTG 2715 36383.17 883224.4 16
GGCGC 6270 6757.4707 220204.64 43
TCGGG 11665 5489.865 111236.87 38
GAGCG 11665 4392.041 166019.17 9
GCGGC 3865 4165.49 93156.305 10
CGGCG 3515 3788.2795 95882.625 11
CGGTG 8005 3767.3699 207335.62 39
GGCCG 3410 3675.116 75343.4 43
GGTCG 7410 3487.3472 177053.4 43
GGAAG 26030 3423.8972 81678.63 5
GCCGG 3025 3260.1836 102865.445 13
GGGCC 2970 3200.9075 72591.3 11
CCGGG 2905 3130.8538 105577.35 14
GGGAA 22515 2961.5466 137077.2 21
CGGGT 4300 2023.6967 97295.46 15
CGGGA 4545 1711.2579 64020.273 3
TAGGG 9915 1630.1766 82369.64 19
GTAGG 9590 1576.7416 81474.75 18
AGGGA 11855 1559.3665 78619.96 20
AGAGG 11200 1473.21 41345.98 26
AAGGG 10615 1396.261 19203.121 26
GGGTC 2880 1355.4062 38835.996 42
GTCGG 2815 1324.8153 65259.63 13
GGGTA 8050 1323.5424 40025.207 29
GGTAG 7665 1260.2424 37538.434 30
GCGCG 1155 1244.7974 10132.41 47
TGGGC 2530 1190.6866 41205.887 41
GTGGT 5585 1147.7847 67309.625 41
GGAGA 8495 1117.4034 32163.355 30
GGCGT 2050 964.78564 22205.227 12
AGGGT 5445 895.2407 37438.2 28
AGCGG 2325 875.39606 32932.785 9
CGTGG 1710 804.77234 17119.201 14
GAGTG 4450 731.64764 36792.523 27
CGCGG 670 722.0903 13639.529 46
GCGTG 1505 708.29376 21188.191 13
GAGAG 4875 641.24084 12591.86 6
AGGGC 1550 583.59735 17899.46 10
GTGGC 1190 560.04626 23400.875 41
CGCCG 13410 507.5651 21733.424 46
GAAGG 3440 452.4859 12079.514 18
CTGGG 775 364.736 10189.637 38
GCGGA 845 318.15466 11572.3955 47
ACGGG 800 301.21155 9498.549 2
GTGGA 1770 291.01486 5447.9546 15
CGGGC 270 290.99158 3883.6365 42
TTGGG 1380 283.60666 5554.8984 2
GGTGC 595 280.02316 8309.978 43
TGGGA 1610 264.70844 9832.046 3
GCGGT 545 256.4918 3390.1108 13
GCGCC 6610 250.1868 7651.418 44
GGCAG 650 244.73439 2969.5042 71
TGGAG 1455 239.22409 5566.202 16
GGACG 590 222.14352 8683.372 43
CGGAG 550 207.08295 5710.4204 39
GATCG 35825 206.85901 4086.8274 1
ATCGG 35735 206.33931 4062.0527 2
AGGCG 520 195.7875 3930.2498 9
CTCGG 11770 194.53584 9744.776 37
GACGG 515 193.90494 3527.862 1
TGGCG 410 192.9571 4578.9155 42
GTGTG 885 181.87814 2664.5813 16
GGGCA 475 178.84436 3119.1462 11
CGTCG 10765 177.92511 6673.225 12
TGTGG 855 175.71278 3859.0264 39
GTGCG 355 167.07262 1361.4529 45
TCGGA 28550 164.85204 4141.4585 3
GGGAC 425 160.01863 2849.1995 42
GCCGT 9405 155.4469 8480.636 47
GAGGA 1165 153.24014 3031.5974 31
GCGTC 9195 151.97597 6786.632 11
CAGGG 400 150.60577 4203.794 19
ATGGG 915 150.43991 5273.628 2
TGGTG 730 150.02379 3116.906 39
GGTGT 720 147.96869 2665.6887 40
GTTGG 710 145.91354 2814.3457 1
GATGG 800 131.53215 5451.128 1
AGGAG 980 128.90585 2510.5415 29
GAGGC 340 128.01491 2034.9275 41
AGTGG 760 124.95555 3317.3088 28
CGGAA 26985 124.656586 3544.1392 4
GCAGG 315 118.60205 3118.6895 18
GGGCT 250 117.65679 3052.6108 42
TGGGT 560 115.08674 2073.3438 41
GTCGC 6880 113.713394 6192.274 44
GGCTG 235 110.59737 3052.6445 43
GAGGT 670 110.15818 1717.0577 31
GCCGC 2910 110.142746 2737.5378 44
GCGAG 285 107.30662 674.9073 70
CCGCG 2670 101.0588 424.2875 45
GGGTT 490 100.70091 1704.0522 49
GGTTG 475 97.61823 1406.8912 40
GCTGG 205 96.47855 1694.8826 18
GGAGC 255 96.011185 816.9587 44
GGGAT 565 92.894585 1717.0577 31
AGGTG 550 90.42836 1662.4061 39
TGCGG 190 89.41915 1017.4141 40
GGCGA 230 86.59831 680.7989 44
CGAGG 220 82.833176 1087.6991 39
GGTGA 470 77.27514 1243.3866 30
CGCGC 2000 75.699486 205.29051 46
GCCCG 1925 72.860756 3544.683 46
CGCGA 5310 70.21378 143.44054 47
GTGAG 420 69.054375 1065.8685 16
TCGCG 3985 65.864525 137.4633 45
AGAGC 14125 65.250114 2156.2432 8
GGAGT 380 62.477776 710.48035 32
GGATG 375 61.655697 710.96783 43
CGCGT 3450 57.021988 358.67468 48
TCGGT 7830 56.5126 3060.2148 38
GTCGT 7675 55.393898 2251.1704 13
CGACG 4115 54.412384 195.26245 12
TGAGG 310 50.968704 1302.7258 17
AGCGT 8715 50.32173 2339.5112 10
CGTGT 6925 49.980812 2375.929 15
ACGCG 3770 49.850468 105.19286 45
AAGAG 30685 49.520374 933.7479 25
TCGTG 6555 47.310356 2266.6538 14
GTCCG 2685 44.37798 136.98653 43
GGAAA 23745 38.320393 1434.6104 22
GAAGA 23580 38.05411 888.60944 6
TGGTC 5120 36.953323 2239.2812 42
GGCCC 915 34.632515 985.4421 45
CGGCC 795 30.090546 1464.4764 45
GCCGA 2115 27.966509 922.8008 47
CCCGG 715 27.062563 778.4566 37
GTGTA 10695 26.96679 809.12463 16
TGTAG 10610 26.752468 828.19336 17
TCCGG 1490 24.626883 1258.3524 37
CCGGA 1665 22.016186 846.2991 47
GAAAG 12795 20.648956 916.8798 23
GACGC 1555 20.561665 349.07437 44
CCGTG 1080 17.850359 565.49915 14
GTAGA 8840 17.832218 921.8488 31
GACCG 1220 16.131983 262.088 1
GCTCG 975 16.114908 101.39044 35
AGCGC 1200 15.867522 738.1489 10
GCGAC 1185 15.669178 133.35588 11
TCGGC 945 15.619063 254.78348 71
CCGGT 935 15.453782 364.65253 48
GCGCA 1155 15.2724905 157.78459 47
AAAGG 9040 14.589024 258.4556 7
GCGAA 3065 14.158697 28.309017 22
TTCGG 1920 13.857497 507.82587 37
TGGCC 815 13.470409 595.5869 42
CCGGC 350 13.247409 383.216 47
GAACG 2800 12.934537 131.51538 1
GCGTA 2235 12.905229 102.30739 47
GCGCT 740 12.230803 41.477608 70
AAGCG 2630 12.149225 382.43814 9
GCGAT 1950 11.259596 48.033283 48
AGTGT 4460 11.245619 556.8842 28
AGAGT 5335 10.761867 460.28525 26
GCGTT 1460 10.537471 85.787445 11
CGGCA 785 10.380004 203.83444 72
GACGA 2195 10.139752 24.956923 13
GAGAT 4955 9.995321 290.5749 31
GTCGA 1730 9.989283 43.673138 13
CGGAT 1700 9.816058 520.0125 48
GCACG 740 9.784973 52.143505 71
GGTCC 560 9.255743 273.97308 43
GTACG 1565 9.036547 29.132166 1
GACGT 1550 8.949935 68.62922 13
CCCCC 186645 8.713081 10.709847 17
GTTCG 1205 8.697022 161.26192 1
CCCCG 6530 8.680052 49.98701 46
ACCGG 625 8.264335 252.56975 2
GGATC 1410 8.141554 364.28726 49
GTGCC 485 8.016133 268.96942 44
GGCTC 475 7.850852 220.46652 49
CGCCC 5735 7.623293 119.205574 45
CGGCT 450 7.4376497 298.89554 48
AGAGA 4525 7.302581 63.061684 72
CCGCC 5395 7.171344 92.28817 45
CGGTC 430 7.107087 190.67375 49
TCGCC 11830 6.866797 218.71211 45
TGCCG 405 6.693885 239.05502 46
CCCGC 4870 6.4734836 37.971603 47
TCTCG 22645 5.7398705 144.78766 36
AACGG 1235 5.7050548 114.873405 2
TCTGG 750 5.4130845 153.64986 37
ACGGC 390 5.156945 161.91655 10
CTCTC 576910 5.1355157 7.1515055 71
CCGTC 8785 5.0993075 12.8063545 48
TGCGC 305 5.0410733 47.816788 44
CAGAG 1085 5.0121336 62.93163 70
GAGAA 3025 4.8818355 140.06183 31
CGCAG 365 4.8263717 47.812626 46
CCCGA 10355 4.8086615 33.751488 47
CGGTT 665 4.7996016 88.466774 49
CGCTG 290 4.7931523 47.81101 46
GAGCA 1030 4.7580614 101.42924 9
CGTGC 285 4.710511 77.3874 15
AGAAG 2715 4.3815484 39.919632 71
CTGTG 600 4.3304677 106.78196 38
GGCCT 260 4.297309 107.15259 12
CGTGA 735 4.2440014 189.24886 15
CCGTA 20625 4.182427 103.780815 48
CTGCG 250 4.1320276 35.858253 46
CGTCC 6960 4.039975 6.4522114 57
CTCCG 6885 3.996441 32.25604 36
CTCGC 6855 3.9790273 7.0750513 72
GCAGA 855 3.949653 57.96183 72
TGTCG 540 3.8974206 52.016403 43
GTGTC 535 3.8613336 39.007744 41
ACGGA 835 3.8572636 124.809616 3
CGAGA 830 3.8341658 34.7771 72
GCTGC 230 3.8014653 89.65648 44
CCTCG 6475 3.7584538 12.148377 36
CCGAT 17820 3.613617 22.88312 48
AGGAA 2225 3.5907717 76.74879 20
TCCCG 6165 3.5785124 12.9861965 36
ACGTG 610 3.5222328 60.307514 14
CGACC 7555 3.5083961 5.541455 45
CCGAC 7550 3.5060737 6.5503564 48
GGTCT 485 3.5004613 62.41969 43
CGCTC 6010 3.488542 12.974151 49
CCGCT 5955 3.4566166 24.563007 48
TCCGT 13495 3.420603 8.4142275 37
TACCG 16865 3.419958 5.234155 71
TCCGC 5865 3.4043756 7.1369987 44
CCTGG 205 3.3882625 89.45633 37
CAGCG 255 3.3718488 37.92269 72
CCCGT 5770 3.3492322 26.236279 47
CCGAA 20390 3.3079295 10.327089 48
CTTGG 455 3.283938 106.773636 37
ACCGA 20225 3.2811615 4.6529455 61
AGACG 710 3.279829 28.284515 12
ACCCG 7030 3.2645962 5.7091017 46
CGGAC 240 3.1735046 28.58914 42
CACGG 235 3.10739 85.881 37
AGATC 43715 3.0969193 39.690624 33
ACGAG 665 3.0719528 24.84079 72
AGCGA 660 3.0488548 44.920124 10
ACCGC 6455 2.9975774 6.4926205 72
TTCCG 11465 2.9060552 11.341548 36
TGGAA 1440 2.904796 71.20616 4
CGAAA 51240 2.9041107 5.8794255 4
GCCTG 175 2.8924196 101.19968 13
CGCCT 4850 2.8152127 11.123976 46
AAGTG 1380 2.7837627 85.04817 27
AGCCG 210 2.7768166 71.71894 46
GCCCC 2075 2.7582097 22.59302 44
TGTGT 875 2.7577307 18.16216 15
ATCCG 13505 2.7386026 5.9270735 36
ACGCC 5880 2.7305584 12.594215 45
GGTTC 375 2.7065423 83.255585 50
CGTTG 375 2.7065423 57.189083 12
ATTGG 1070 2.6979399 75.419426 1
TCGAG 465 2.6849804 41.591385 14
CACCG 5760 2.6748328 5.541187 46
GAGAC 575 2.6561992 34.77697 71
TTTCG 23985 2.6547878 7.8682323 36
GGACC 200 2.644587 42.884186 43
GGCAC 200 2.644587 33.354366 43
CCGCA 5675 2.63536 16.45987 48
GTGAA 1300 2.622385 59.573925 16
AAGGA 1620 2.6144047 46.51442 20
CGCCA 5600 2.6005318 5.0374427 46
CGTAT 29100 2.5768416 47.087376 49
CGGTA 445 2.5694973 20.884035 48
CTTCG 10060 2.549927 7.774448 36
TGTGC 350 2.526106 15.660369 44
TGAAG 1225 2.4710937 64.65938 17
AGTCG 425 2.4540148 35.35445 12
CGATC 12080 2.449635 11.258303 49
TTGCG 335 2.4178445 18.111784 71
CCGTT 9490 2.4054482 14.759848 48
AGGTC 415 2.396273 47.856174 42
ACTCG 11805 2.3938692 10.390769 35
TAGAG 1185 2.390405 32.693672 19
TGCGT 330 2.381757 13.048971 47
CTCGT 9385 2.3788335 6.5424967 72
TTGGA 940 2.370153 61.766262 3
GTTGT 750 2.3637693 22.711712 41
CGCTT 9320 2.362358 7.949994 49
CGTCA 11620 2.3563542 5.4777555 12
GGTAA 1165 2.350061 42.8598 30
CACGC 5060 2.3497663 7.491485 72
TGGAC 405 2.3385315 114.438675 42
CCGAG 175 2.3140135 47.622593 14
CGCAC 4980 2.3126159 6.1598244 70
GAGCT 400 2.3096607 49.897453 33
TTTGG 725 2.2849772 35.255463 36
GGTCA 395 2.28079 52.0365 50
GCAGC 170 2.247899 33.472885 44
GAGCC 170 2.247899 28.688961 45
CGTTT 20125 2.2275422 6.3047066 49
GTCTG 305 2.201321 31.194046 13
TAGCG 380 2.1941776 18.705584 9
CGTAG 375 2.1653068 24.955017 17
ATCGA 30270 2.1444297 6.6285896 72
GAGTC 370 2.136436 27.036957 11
CACGA 13125 2.1293075 6.513859 72
ACACG 13105 2.126063 6.746473 71
AAGGC 460 2.1249597 61.511948 8
TTGTG 670 2.111634 21.566648 14
TGCGA 360 2.0786946 16.559963 72
GTGTT 655 2.0643585 19.296654 16
GCTGT 285 2.056972 23.488148 47
GACAG 440 2.0325701 29.808945 72
CTGGT 280 2.0208848 36.462135 38
AGATG 1000 2.0172193 61.468594 72
CGAAG 430 1.9863752 19.872555 71
GAGTA 975 1.9667891 51.57705 29
CATCG 9690 1.9649804 6.646343 12
GTGCA 340 1.9632115 20.795885 16
CGATT 22045 1.9521124 7.118973 49
GTGCT 270 1.9487103 10.40328 43
TACGG 335 1.9343408 27.08496 37
TGCAG 335 1.9343408 22.875431 17
AGGCC 145 1.9173256 33.33897 11
TGCTG 265 1.9126232 25.87512 59
ATAGG 940 1.8961861 39.955677 18
TGTTG 600 1.8910154 23.836998 17
CGAGT 325 1.8765993 51.991447 15
TCAGG 325 1.8765993 27.08496 37
GGCCA 140 1.851211 42.862484 12
ATGCG 320 1.8477285 10.350379 68
AGTAG 910 1.8356699 39.227615 30
TAGGA 895 1.8054116 55.942513 19
GGAAC 390 1.801596 41.587395 32
GTAAG 890 1.7953252 49.399746 18
GTGAC 310 1.789987 10.403516 42
CGCAT 8815 1.787544 8.434484 48
CGAGC 135 1.7850963 18.961273 71
GTTGC 245 1.7682742 36.540863 44
GGACT 305 1.7611163 64.450874 33
CTGGC 105 1.7354515 41.749546 38
AGTGA 860 1.734809 50.148533 28
TGACG 300 1.7322453 14.565083 43
GGATT 685 1.7271858 65.44836 49
TCACG 8495 1.722653 5.5249414 71
CACGT 8440 1.7114999 5.0887804 72
GGTTT 540 1.7019142 9.089743 49
GGCAT 290 1.6745039 41.593468 12
TTGGT 530 1.6703973 32.98165 38
GTAGC 285 1.6456332 31.321796 44
CTGGA 285 1.6456332 29.11521 15
GCAGT 285 1.6456332 18.791153 47
CGATG 285 1.6456332 14.595648 39
CAGTG 280 1.6167626 16.680742 39
GCATG 280 1.6167624 31.1951 13
CAGGA 345 1.5937197 13.248786 69
AGGAC 345 1.5937196 46.57788 32
ACTGG 275 1.5878918 18.751127 37
TAAGG 780 1.573431 47.95072 19
ACGGT 270 1.5590211 33.337936 38
AGCAG 330 1.5244275 19.872555 71
GGACA 320 1.4782326 16.646372 43
GATGT 570 1.4372203 17.175169 60
AAGGT 690 1.3918815 31.257023 27
CTGAG 240 1.3857964 12.419928 71
GATCT 15260 1.3512924 49.394283 34
GGTTA 535 1.3489699 19.08744 50
TGTGA 535 1.3489698 10.847033 72
GAAGC 290 1.3396485 11.592668 70
GTTTG 425 1.3394693 27.25911 1
GATGC 230 1.328055 18.79308 44
AGTGC 225 1.2991842 14.556959 10
GGTAC 225 1.2991841 20.807264 43
TGGTT 410 1.2921939 24.985523 42
TGAGA 640 1.2910204 19.525318 72
GGAAT 630 1.2708482 17.437693 5
TGGCT 175 1.263053 33.810284 42
GAAGT 620 1.2506762 20.340244 31
GATAG 620 1.2506762 21.081802 1
ATGTG 495 1.2481124 8.135245 71
GGCTT 170 1.2269658 28.621603 49
ACAGG 265 1.2241616 11.645379 18
GTAGT 485 1.2228979 16.27121 60
ATGGA 605 1.2204179 18.174845 2
GACTG 210 1.2125719 14.566085 1
TTAGG 480 1.2102908 37.23017 18
CCAGG 90 1.1900641 38.169327 37
GAGTT 470 1.1850765 15.433926 33
AGGTA 585 1.1800735 26.16445 28
ATGAG 575 1.1599011 15.90946 71
GATTG 460 1.159862 41.798717 1
GCTGA 200 1.1548303 8.352479 44
GCCCT 1970 1.1434988 13.012953 46
AATGG 565 1.1397289 17.447008 1
CTAGG 195 1.1259595 43.75263 37
GCCAG 85 1.1239495 23.812494 13
GGATA 555 1.1195569 14.530711 15
GGCAA 240 1.1086745 24.978544 22
GTCAG 190 1.0970888 8.28038 62
CATGG 185 1.0682181 10.417293 37
GTGAT 420 1.0590044 7.2679935 41
AGTTG 400 1.0085757 7.2676573 28
GTATC 11325 1.002843 41.241753 50
TGGTA 395 0.9959684 9.994547 42
CAGGT 170 0.9816059 20.83621 38
TGGCA 170 0.98160577 8.322812 42
GATGA 485 0.9783516 10.124652 60
GCAAG 210 0.97009027 14.987658 23
GTATG 380 0.9581468 8.178009 1
GTTGA 370 0.93293244 12.655386 60
CAGGC 70 0.92560554 9.529714 42
GGTAT 365 0.92032534 11.804207 30
CAAGG 195 0.90079814 13.309004 18
TGATG 355 0.89511085 11.75143 59
GAATG 440 0.88757646 13.812215 1
TAGTG 350 0.88250375 7.2313294 71
GAAAA 44495 0.8810088 7.280607 23
GCTCC 1505 0.8735866 5.2310557 50
GTTAG 345 0.8698965 16.345922 17
AGGAT 430 0.8674043 18.160257 32
GTCCC 1480 0.8590752 8.60638 44
TATGG 325 0.8194677 9.097984 37
TAGGT 325 0.8194677 20.017126 38
TGAGT 315 0.7942533 6.3274126 71
AGGTT 310 0.78164613 9.993029 28
TGGAT 310 0.7816461 8.177357 42
AGGCA 165 0.7622137 8.323093 42
TTGGC 105 0.7578319 10.417753 38
GCTTG 105 0.7578318 7.7985115 12
AGCTG 130 0.75063974 6.2390203 12
TAGAT 22180 0.6861534 13.668886 32
GATCA 9680 0.6857642 6.679557 70
GCTAG 110 0.6351567 6.2104225 63
GGCTA 105 0.6062859 6.238129 31
AAAGA 29850 0.59103525 10.713913 24
AGGCT 100 0.57741517 10.391991 9
GCATC 2645 0.5363646 5.6291513 49
TCAGA 6740 0.47748452 9.803201 72
CACAG 2630 0.4266727 7.4443836 71
ACAGA 6890 0.39050204 5.973552 72
ATCAG 5345 0.37865797 8.330151 71
TGCCC 420 0.2437916 7.9760656 45
AAAGC 4115 0.23322435 5.4256353 8
TTTGT 4440 0.21460181 5.8566375 72
Produced by FastQC (version 0.10.1)