FastQCFastQC Report
Mon 3 Feb 2014
filtered_BS_CgLarv_T3D5_CAGATC_L007_R2.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename filtered_BS_CgLarv_T3D5_CAGATC_L007_R2.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4421486
Filtered Sequences 0
Sequence length 76
%GC 17

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 72055 1.2233976E7 2.26787696E8 40
CGGGG 26410 194013.8 6797023.0 39
GGGCG 24080 176897.08 4402633.0 43
GGGGC 23130 169918.17 3462367.5 41
GGCGG 14100 103581.76 3243962.0 12
GGGGA 35370 94993.586 2224623.2 20
AGGGG 34050 91448.45 1263084.0 27
GAGGG 33745 90629.31 2977200.5 27
GGTGG 27110 87360.445 4390211.0 40
GCGGG 10295 75629.38 3249250.5 13
GGGAG 21335 57299.637 982661.5 29
GTGGG 11685 37654.254 873900.44 18
GGGGT 10525 33916.223 1078704.2 41
TGGGG 8515 27439.107 914610.4 19
GGAGG 9820 26373.678 911229.44 17
GGGTG 6650 21429.25 705673.2 16
GGCGC 13880 4411.7847 169894.66 44
TCGGG 28215 3933.925 108121.766 38
GAGCG 29755 3457.6406 163999.06 9
CGGTG 21365 2978.852 184894.56 39
GGTCG 21205 2956.5437 167017.94 43
GGCCG 8415 2674.7239 66207.734 43
GGAAG 59460 2526.0378 84047.71 5
GCGGC 7925 2518.9768 62510.566 11
GGGCC 7690 2444.2812 60449.773 11
GGGAA 56425 2397.1018 110044.21 21
CCGGG 7445 2366.4077 97537.42 14
CGGCG 7355 2337.801 63080.855 12
GCCGG 7015 2229.7314 96281.195 13
CGGGT 12460 1737.2571 99383.99 15
TAGGG 27150 1383.9199 78230.7 19
AGGGA 32530 1381.9713 72751.36 20
GTAGG 26505 1351.0421 76864.48 18
GGGTA 24185 1232.7845 44863.52 29
GTCGG 8750 1219.9838 68900.24 13
GGTAG 22915 1168.0487 42404.41 30
AGAGG 27095 1151.0763 47261.895 26
GGGTC 8040 1120.9908 40497.504 42
AAGGG 24935 1059.313 12979.796 8
GTGGT 15705 960.5172 60185.266 41
CGGGA 8020 931.95355 27711.92 3
AGGGT 15955 813.2759 41269.355 28
TGGGC 5510 768.2412 35372.508 42
GCGCG 2225 707.2206 6067.8755 11
GGCGT 4925 686.6766 17225.06 13
GGAGA 16050 681.85175 16661.043 31
GAGTG 12240 623.9109 30897.945 27
AGCGG 5090 591.47675 22391.309 10
CGCCG 35910 493.85666 25188.586 46
AGGGC 4015 466.5578 13978.873 10
CGTGG 3285 458.01678 11048.244 15
CGCGG 1440 457.70682 9539.856 46
GCGTG 3165 441.2856 15416.694 14
GTGGC 2980 415.49164 18036.062 41
GAGAG 8790 373.42538 5412.021 6
GAAGG 8390 356.4322 11649.6045 6
CTGGG 1630 227.26555 6138.1367 38
GCGCC 15585 214.33463 7216.3325 45
CTCGG 32160 194.00937 11514.788 37
GCGGA 1665 193.47914 6345.2188 47
GTGGA 3745 190.89427 3451.546 16
TGGGA 3720 189.61995 8061.1006 3
TTGGG 2920 178.58713 2957.098 37
CGGGC 555 176.40784 2405.168 41
ACGGG 1485 172.56247 5402.236 2
CGTCG 27975 168.76282 7509.3354 12
GCCGT 27580 166.37994 9914.946 47
GGTGC 1165 162.43213 4773.376 43
TGGAG 3145 160.31041 3488.288 17
GATCG 72475 159.84167 4824.332 1
ATCGG 71280 157.20613 4870.623 2
GCGTC 24785 149.51874 7709.5005 11
GCGGT 1060 147.79234 2873.8933 47
TCGGA 60695 133.86119 4916.327 3
GGACG 1145 133.0532 4690.228 43
GACGG 1135 131.89117 3643.2236 1
GGCAG 1130 131.31015 1207.9794 71
ATGGG 2565 130.74603 6355.9604 2
GTGTG 2110 129.04753 2268.92 16
TGGCG 925 128.96971 3466.908 42
GATGG 2485 126.668175 6410.8154 1
AGGCG 1070 124.337944 2927.8079 9
TGTGG 1995 122.01413 2533.2773 17
GTCGC 19615 118.33003 7234.3857 44
TGGTG 1820 111.31114 2186.6428 39
CGGAA 59460 109.29506 4317.9146 4
CGGAG 935 108.650444 3105.468 39
GTGCG 760 105.96431 1306.3978 43
GTTGG 1720 105.195145 1563.8937 18
GGGAC 865 100.51618 2093.8088 42
GGGCA 860 99.93516 920.8279 11
GGTGT 1615 98.77334 1674.8433 40
GAGGA 2230 94.73704 1407.5447 31
AGTGG 1775 90.47727 3305.9565 28
GCCGC 6440 88.56689 2879.6821 44
CAGGG 735 85.409706 2050.8813 19
AGGAG 1955 83.05422 1453.4148 29
GGGCT 590 82.261765 1557.5964 42
GCGAG 655 76.11341 499.85526 72
GGCTG 535 74.59329 1909.3506 43
GCAGG 615 71.46526 1632.1727 18
GGTTG 1165 71.25136 947.219 16
GAGGC 600 69.72221 1130.0287 10
CCGCG 4895 67.319084 338.18787 45
GGGTT 1100 67.275955 815.0674 15
GGAGC 565 65.655075 462.26712 44
AGAGC 35245 64.784805 2747.8142 8
TGGGT 1030 62.994762 793.36127 41
GAGGT 1180 60.148262 991.82745 41
TCGGT 21085 55.795803 3430.9614 38
CGCGC 3870 53.22265 198.92271 46
AGCGT 24010 52.953407 2743.6575 10
GTCGT 19705 52.144 2355.1648 13
CGAGG 445 51.71064 587.52094 39
AAGAG 76825 51.626564 1205.7543 25
GGCGA 440 51.129616 462.26712 44
CGCGA 10130 50.931915 79.303894 58
AGGTG 985 50.208515 754.7453 39
TGCGG 360 50.19362 352.46783 39
GCCCG 3635 49.99078 2262.7456 46
CGTGT 18640 49.325764 2567.7344 15
GGGAT 960 48.93418 697.5641 31
GCTGG 350 48.79935 351.6717 40
GGTGA 950 48.42445 477.27905 30
GTGAG 930 47.404987 440.62286 16
TCGCG 7760 46.813206 100.352486 45
TCGTG 17505 46.322292 2370.3394 14
GGAAA 58505 39.31548 1614.3804 22
TGGTC 14635 38.7276 2522.3232 42
CGACG 7440 37.40705 114.08859 12
CGCGT 6075 36.648228 135.279 48
GAAGA 53150 35.716915 1148.2135 6
GGAGT 695 35.426308 348.71588 33
ACGCG 6785 34.113823 64.882065 71
GGCCC 2225 30.59958 654.2074 43
GGATG 580 29.5644 422.26645 17
GTCCG 4745 28.624828 97.828606 1
GTGTA 29075 28.128246 912.23615 16
TGTAG 28680 27.746109 940.46686 17
TGAGG 500 25.486551 660.9388 17
GAAAG 35775 24.040876 1170.3889 23
GCCGA 4585 23.052599 909.07623 47
CCCGG 1530 21.041512 734.41376 37
GTAGA 25430 20.504213 1157.7172 31
CGGCC 1445 19.87254 984.7235 45
AGCGC 3415 17.170038 858.3506 10
TCCGG 2790 16.831038 814.089 37
CCGGA 3090 15.535994 489.083 47
AAAGG 20380 13.695403 226.60078 7
GACGC 2715 13.6505575 207.28404 44
CCGTG 2175 13.12097 517.1189 14
GACCG 2440 12.267904 239.16696 1
TGGCC 2005 12.095423 563.05865 42
AGTGT 12325 11.92367 585.27124 28
TTCGG 4440 11.74927 433.4883 37
GCTCG 1935 11.673139 63.129074 35
GCGCA 2290 11.51373 129.08882 47
AGAGT 14055 11.332551 497.91412 26
TCGGC 1800 10.858733 160.02213 71
GCGAC 2130 10.709278 101.41555 11
GCGAA 5640 10.367039 20.520521 29
AAGCG 5495 10.10051 392.9947 9
GAACG 5295 9.732884 109.29649 1
GCGTA 4400 9.704082 145.17944 48
CCGGT 1545 9.320414 208.98189 38
CCCCC 353425 9.099218 11.590586 6
GCGCT 1495 9.018783 50.174644 47
GAGAT 10505 8.470184 289.49072 32
GCACG 1635 8.220502 28.989935 1
GCGAT 3655 8.061005 19.764772 69
GGTCC 1290 7.782092 310.8843 43
CCCCG 12600 7.497503 64.766655 46
GACGA 4050 7.4444156 17.790268 72
TCGCC 28475 7.4324346 312.81207 45
GTTCG 2805 7.42268 133.50656 1
CGGAT 3080 6.7928576 311.89655 48
GTCGA 3030 6.6825833 29.391659 13
CGGCA 1310 6.586457 135.17143 72
GTACG 2980 6.572309 23.843481 1
GCGTT 2475 6.549424 40.03254 11
CCGCC 10865 6.4651093 118.78152 45
CCGGC 465 6.3949695 159.14215 47
CTCTC 1289955 6.3903375 8.754779 70
CGCCC 10730 6.384779 95.32647 45
AGAGA 9480 6.37058 68.65286 72
ACCGG 1210 6.0836744 206.56151 2
TCTCG 52855 6.0516615 213.97116 36
GACGT 2705 5.9658046 44.484673 13
CCCGC 9510 5.65883 29.909605 47
GTGCC 870 5.248388 185.44113 44
GGATC 2370 5.2269707 213.88701 49
GAGCA 2550 4.687225 129.67503 9
CCGTA 47755 4.5570235 156.3481 48
TGCGC 735 4.433983 45.81487 44
CCGTC 16590 4.330258 12.744803 48
TCTGG 1635 4.326589 121.26214 37
AACGG 2340 4.3012176 117.912415 2
GGCTC 710 4.283167 86.99556 49
CCCGA 19240 4.1854897 29.893347 47
CGGCT 675 4.0720253 146.18861 48
TGCCG 665 4.0116987 176.69756 46
GAGAA 5750 3.8640122 88.81701 31
CAGAG 1975 3.6303012 49.41724 71
CGGTC 600 3.6195781 76.121124 49
CGGTT 1350 3.5724132 67.73555 49
TCTCT 1594605 3.465162 4.9144406 72
ACGGA 1860 3.418917 124.48648 3
CTCCG 12975 3.3866844 23.358227 36
CTCGC 12430 3.2444305 7.578714 72
AGGAA 4825 3.2424102 68.27757 20
CCTCG 12390 3.23399 10.172131 36
CTGTG 1220 3.228403 50.64997 39
CGTCC 12305 3.2118037 4.6066427 12
CGCTG 525 3.1671305 26.177416 46
CGAGA 1720 3.1615791 37.55723 72
CCGAT 32945 3.1437786 21.847319 48
CGCAG 625 3.1423936 39.998352 46
GTGTC 1185 3.1357846 25.747723 42
AGAAG 4665 3.1348898 26.979277 71
ACGGC 620 3.1172545 83.29985 10
CCGAC 14190 3.0869074 4.2303243 34
TCCCG 11765 3.070855 13.751214 36
AGCCG 610 3.066976 67.26996 46
TCCGT 26515 3.035849 6.4447603 37
CGTGA 1375 3.0325253 123.922714 15
CCGAA 38125 3.0321178 11.391042 48
ACCGA 38065 3.0273464 3.820313 60
CGCTC 11590 3.025177 9.786584 49
CACGG 600 3.0166981 79.822784 37
CCGCT 11305 2.9507875 20.486092 48
CCCGT 11225 2.9299061 31.336842 47
GGCCT 485 2.9258254 58.666683 12
AGATC 83040 2.8969753 64.18823 33
TGTCG 1050 2.7785432 45.774666 43
CCTGG 460 2.7750096 49.97368 14
CTGCG 455 2.7448466 15.137228 71
CAGCG 545 2.7401671 23.429636 71
CGAAA 93985 2.7326868 5.1105633 4
CGTAT 63880 2.6739209 70.444145 49
CGTGC 440 2.654357 26.07429 15
ACCGC 12175 2.6485624 5.2246737 72
GCCCC 4415 2.6271014 29.266146 44
GCTGC 415 2.5035412 65.449814 44
TTCCG 21585 2.4713862 9.378539 36
CCGCA 11330 2.46474 21.086668 48
GCAGC 490 2.4636366 41.820465 44
AAGTG 3050 2.4592156 92.98422 27
CTTGG 925 2.4477644 86.88862 37
AAGGA 3615 2.4292877 57.382217 20
ACGCC 11140 2.423407 10.148329 45
ATCCG 25350 2.4190252 5.096183 36
ACGAG 1315 2.4171374 24.379171 71
AGCGA 1290 2.3711843 40.38418 10
ACGTG 1075 2.3708835 39.717194 14
CGCCT 9030 2.3569758 11.137513 46
CACCG 10765 2.3418295 5.1918726 46
GCAGA 1260 2.3160405 31.627142 72
AGACG 1255 2.3068497 16.47247 72
CGGTA 1045 2.3047192 34.30064 48
TTTCG 45665 2.293471 7.5572996 36
GGCAC 455 2.2876623 38.050106 43
GAGCC 450 2.2625237 36.193886 9
TCGAG 1020 2.2495825 23.035973 14
CTTCG 19535 2.2366703 5.784121 36
CGTCT 19470 2.229228 5.8202024 49
TAGAG 2745 2.2132943 37.754154 19
CCGTT 19285 2.2080464 16.357527 48
GGTTC 820 2.16991 65.82121 50
GGTAA 2635 2.1246011 45.878773 30
CGATC 22255 2.1236846 9.529554 49
CACGC 9740 2.1188498 8.343882 72
CGTCA 22075 2.1065083 7.4239883 12
TGGAA 2610 2.1044436 52.274143 4
CGTTG 790 2.0905232 37.171806 12
GGTCT 785 2.0772922 32.39544 33
CCACG 9305 2.0242195 5.6925354 71
ACTCG 20920 1.9962922 8.126419 35
CGTTT 39445 1.9810789 7.1883745 49
GAGAC 1075 1.9759871 27.014761 71
CGCTT 17015 1.9481416 7.1410995 49
ACACG 24395 1.940158 6.8420963 71
CACGA 24390 1.9397603 6.3004518 72
GACAG 1040 1.9116523 17.790266 72
CGGAC 380 1.9105755 25.366222 42
CGAGC 380 1.9105753 18.023102 70
GCCTG 315 1.9002783 58.66662 13
AAGGC 1030 1.8932713 59.53377 8
TGTGT 1615 1.8746562 13.797092 15
ATCGA 52965 1.8477638 5.089733 72
GAGCT 835 1.8415701 28.58784 33
GTGAA 2280 1.8383645 45.29731 29
CGTAG 830 1.8305426 23.830912 17
CCGAG 360 1.8100187 16.297832 14
GGACC 355 1.7848793 36.238197 43
TTTGG 1525 1.7701864 31.414576 36
GCTGT 665 1.7597443 17.074903 60
CGATT 40910 1.7124311 6.7003427 49
TGCGT 645 1.7068197 11.382883 72
CATCG 17700 1.6890236 8.970654 12
ATAGG 2085 1.6811359 57.787178 18
TGTGC 630 1.667126 15.25791 42
TGAAG 2045 1.6488839 48.208378 17
AGTCG 740 1.6320502 19.064882 12
CAGTG 740 1.63205 12.64921 71
ATTGG 1680 1.6252953 35.21343 2
GAGTA 2015 1.6246951 42.393642 29
GAGTC 735 1.6210228 18.271135 11
TAGCG 725 1.598968 18.973816 71
GTGCA 720 1.5879406 13.502112 29
GATCT 37870 1.5851812 75.95681 34
GGCCA 315 1.5837662 48.895103 12
TAGGA 1935 1.560191 55.179146 19
GTTGT 1340 1.5554422 16.31229 41
GGTCA 705 1.5548584 27.031466 50
ACGGT 705 1.5548584 23.079714 38
CGCAT 16220 1.5477945 9.873582 49
GTAAG 1915 1.5440649 55.173687 18
GTCTG 580 1.5348146 18.10932 13
AGGTC 695 1.5328039 37.354836 42
TAAGG 1900 1.5319704 56.34081 19
TTGGA 1570 1.5188771 45.654438 3
AGATG 1810 1.4594034 34.39433 72
AGGCC 290 1.4580708 23.542896 11
TGTTG 1255 1.4567763 18.724964 59
ATGCG 660 1.4556123 12.649211 71
CTGGT 535 1.4157342 27.692146 38
AGCAG 770 1.415358 13.837062 70
TACGG 640 1.4115028 19.870361 2
AGTGA 1725 1.3908681 40.673203 28
GTGAC 620 1.3673931 7.9057555 71
TTGCG 510 1.3495785 10.434276 71
GTTGC 510 1.3495781 34.451714 44
AGTGC 600 1.3232839 11.06806 71
CGAGT 600 1.3232838 19.065033 15
TGGAC 600 1.3232837 54.84007 42
AGTAG 1640 1.3223325 32.521667 30
GGAAC 715 1.314261 15.886545 32
GTGTT 1130 1.3116789 13.378695 16
TTGTG 1120 1.3000714 14.632685 14
GGATT 1335 1.2915292 42.20255 49
GTGCT 480 1.2701913 6.6781526 49
GTATC 30150 1.2620336 64.34128 50
TGCAG 565 1.2460922 13.504183 17
GCAGT 555 1.2240375 16.748302 47
AGGAC 660 1.2131641 24.49176 32
TTGGT 1030 1.1956013 26.807714 38
TGCGA 535 1.1799281 8.696362 72
CAGGA 640 1.1764015 7.9459453 3
CTGGA 525 1.1578733 19.862343 3
TGACG 525 1.1578733 8.696685 59
GGTTT 975 1.1317583 11.717568 49
CGAAG 615 1.1304482 5.93031 59
GGCAA 600 1.1028763 15.240657 22
TCAGG 495 1.0917091 14.324117 37
GATAG 1320 1.0643164 15.690517 1
CCAGG 210 1.0558442 18.14154 37
GGCAT 475 1.0475996 14.298156 10
GTTTG 885 1.0272884 21.33369 1
CGATG 465 1.025545 11.859117 59
GGACT 465 1.0255449 28.587835 33
GATTG 1040 1.0061352 39.047005 1
ATGTG 1040 1.0061352 7.2825575 71
TGGCA 455 1.0034902 17.48524 42
ACAGG 540 0.9925888 6.632377 37
GAAGT 1225 0.98771787 24.391333 31
TGTGA 1020 0.9867863 8.32295 72
GATGT 1010 0.97711205 16.646467 60
GGACA 530 0.9742074 7.28659 43
GGATA 1200 0.96756023 8.712496 15
GCAAG 525 0.9650168 7.9520535 23
AAGGT 1190 0.95949733 15.691088 27
GCCAG 190 0.95528764 16.298351 13
GGTAC 430 0.9483533 14.306397 43
TGAGA 1175 0.9474027 13.006259 72
GCCCT 3620 0.9448784 13.119943 47
TGCTG 355 0.93941236 11.383307 59
GAAGC 510 0.93744504 9.883617 70
GTAGC 425 0.93732595 18.344675 44
TTGGC 350 0.9261813 17.163338 41
GGAAT 1140 0.91918224 7.2600255 5
GCATG 415 0.9152713 12.709907 13
TTAGG 940 0.90939146 26.480015 18
CAGGC 180 0.9050093 5.435044 41
TGGCT 340 0.8997189 30.515823 42
GAAAA 83060 0.88291377 8.2392435 23
ATGGA 1090 0.8788673 15.981719 2
GATGC 385 0.8491072 10.368731 44
AGCTG 380 0.83807975 7.906078 59
ACTGG 375 0.8270525 7.948145 2
CTGGC 135 0.814405 8.707578 38
GGTTA 835 0.80781037 9.059641 16
GTGAT 820 0.7932988 5.228962 41
GTAGT 815 0.7884616 13.936135 31
GTCCC 2995 0.78174335 12.932081 44
GGCTT 295 0.78063846 23.850546 49
CTGAG 350 0.77191556 5.534123 70
TGAGC 350 0.7719155 5.5341225 70
AATGG 940 0.7579222 15.399951 1
TGGTT 650 0.7545056 16.314014 42
AGGCA 410 0.75363225 5.9583216 10
CATGG 340 0.7498609 7.1620593 37
GCTGA 340 0.7498608 7.115446 60
AGGAT 910 0.7337333 12.775919 32
GACTG 325 0.71677876 8.742611 1
AGGTA 885 0.7135758 13.654575 28
GGTAT 730 0.7062294 13.602475 49
TAGAT 45705 0.69942707 21.486942 32
ATGAG 860 0.69341826 8.670811 71
GAGTT 715 0.691718 6.9729323 27
GTATG 695 0.6723691 5.9267764 1
AAAGA 62690 0.6663841 17.474861 24
CAGGT 300 0.6616419 11.937783 38
CTAGG 290 0.63958716 23.077744 37
AGTTG 655 0.6336717 6.936052 59
GTTGA 650 0.6288344 8.323233 60
GTTAG 645 0.6239972 11.847303 17
TGGTA 630 0.6094857 9.064492 42
TATGG 625 0.6046486 6.968425 18
AGGTT 625 0.6046486 7.3202477 28
GCTTG 225 0.5954022 5.72171 1
GAATG 715 0.57650465 8.135823 1
GATCA 16490 0.5752785 5.2523756 70
TGATG 570 0.5514394 7.629656 59
AAAAG 50100 0.5325545 5.0201674 25
GCATC 5460 0.5210208 7.905442 11
TAGGT 535 0.51757914 9.774886 38
TGGAT 520 0.50306755 8.361359 32
CAAGG 250 0.45953187 5.9579897 18
AGGCT 195 0.43006727 7.9382806 9
TCAGA 11060 0.38584474 8.191093 72
ACAGA 13135 0.38191032 5.8262115 72
CACAG 4565 0.36305887 5.7302556 71
ATCAG 8955 0.31240866 7.0155544 71
AAAGC 7855 0.22839022 6.8012686 8
TGCCC 840 0.21925355 7.079332 45
TTTGT 7665 0.16886638 5.0384254 72
Produced by FastQC (version 0.10.1)