FastQCFastQC Report
Mon 3 Feb 2014
filtered_BS_CgM1_ACTTGA_L007_R2.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename filtered_BS_CgM1_ACTTGA_L007_R2.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5543163
Filtered Sequences 0
Sequence length 76
%GC 17

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 100470 2.2212386E7 3.40396704E8 40
CGGGG 32160 278866.38 7781801.5 39
GGGCG 31015 268937.8 6002891.5 42
GGGGC 30260 262391.03 5849513.5 10
GGCGG 19670 170562.84 5918462.0 12
GGGGA 46265 146680.34 3334359.5 20
AGGGG 45690 144857.34 2432422.8 27
GAGGG 39455 125089.66 2835732.0 27
GCGGG 13780 119489.38 5765410.0 13
GGTGG 26900 102169.75 4681842.5 40
GGGAG 30970 98188.484 2099700.2 29
GTGGG 13250 50325.25 1101690.5 40
GGAGG 13150 41691.266 1486762.8 17
GGGGT 10515 39937.36 1104469.6 41
TGGGG 9245 35113.73 886464.94 19
GGGTG 7010 26624.906 622433.9 16
GGCGC 16320 5550.3633 172079.72 43
TCGGG 32200 4796.751 102086.7 38
GAGCG 29490 3667.0374 146678.7 9
GCGGC 9930 3377.1519 72690.266 44
CGGTG 21440 3193.8616 178931.69 39
GGCCG 8800 2992.8433 60424.01 43
CGGCG 8725 2967.3364 60882.64 11
GGTCG 19840 2955.5137 154129.53 43
GGGCC 8520 2897.6162 62597.633 11
GGGAA 60145 2734.509 121144.18 21
GCCGG 7865 2674.854 85869.27 13
CCGGG 7785 2647.646 85254.39 14
GGAAG 58205 2646.3064 78878.95 5
CGGGT 11570 1723.5533 80214.516 15
CGGGA 11575 1439.3341 60428.617 3
AGAGG 31245 1420.5625 41120.844 26
AGGGA 31015 1410.1056 70143.18 20
TAGGG 25435 1385.3591 71467.11 19
AAGGG 29845 1356.9111 19716.371 26
GTAGG 24320 1324.6288 68361.89 18
GCGCG 3660 1244.7509 8240.165 45
GGGTC 8160 1215.5742 35753.504 42
GGGTA 21425 1166.9478 37562.598 29
GTCGG 7505 1118.0005 53333.01 13
GGTAG 20275 1104.3112 34973.867 30
GGAGA 24115 1096.3951 30323.727 30
TGGGC 6580 980.2056 34142.984 42
GAGAG 21500 977.5034 12533.8 6
GTGGT 14705 959.50305 56358.51 41
GGCGT 5630 838.6866 16901.352 12
AGGGT 14640 797.39166 34637.336 28
AGCGG 5985 744.2258 22917.148 9
CGTGG 4575 681.52594 13842.586 14
GAGTG 12485 680.01605 31090.652 27
GCGTG 4310 642.04956 16150.138 13
CGCGG 1730 588.36584 10699.479 46
AGGGC 3960 492.4201 17242.62 10
CGCCG 35140 468.7317 21073.98 46
GTGGC 3055 455.09546 18948.559 41
GAAGG 8790 399.63977 10570.28 6
GCGGA 2430 302.1669 11781.349 47
CTGGG 1940 288.9968 8440.027 38
ACGGG 2150 267.34927 9500.232 2
TTGGG 3980 259.6955 4232.6436 2
GCGAG 2075 258.02316 1604.6787 70
GTGGA 4600 250.54655 4472.865 15
TGGGA 4575 249.18489 9339.585 3
GCGGT 1590 236.85825 3393.79 47
GCGCC 17580 234.49924 6690.929 44
TGGAG 4095 223.0409 4453.1587 16
GGTGC 1480 220.47179 6817.9907 43
GTGTG 3160 206.19038 2044.6185 16
CTCGG 32670 190.88055 9708.816 37
CGGGC 535 181.95128 3062.399 15
CGGAG 1405 174.70966 4131.4463 39
TGGCG 1140 169.82286 4563.135 42
AGGCG 1340 166.627 3312.2441 9
GACGG 1310 162.89655 3584.8503 1
CGTCG 27730 162.01768 5944.9834 12
GAGGA 3455 157.08253 2408.055 28
GGACG 1235 153.5704 5646.4106 43
ATCGG 71440 152.61363 4107.953 2
GCCGT 25635 149.77727 8246.396 47
GGGCA 1190 147.97472 2508.2124 11
ATGGG 2620 142.7026 5790.4253 2
TGTGG 2180 142.24527 3134.0552 39
GATCG 66310 141.65466 4075.456 1
GCGTC 24075 140.66267 6241.938 11
GGCAG 1130 140.51381 1075.5068 43
GTTGG 2120 138.33026 2232.6274 17
GTGCG 920 137.05003 1664.2339 43
GGGAC 1095 136.16162 2240.5967 42
TGGTG 2060 134.41525 2285.7395 39
GGTGT 1925 125.60648 2468.6768 40
TCGGA 57710 123.28291 4159.4663 3
GATGG 2205 120.09895 5220.9883 1
AGGAG 2625 119.34635 3078.1794 29
AGTGG 2100 114.37996 3081.0552 28
GTCGC 18460 107.85597 6034.7344 44
CGGAA 56950 101.55349 3729.6814 4
GAGGC 810 100.7223 1567.5107 10
GGGTT 1540 100.48519 1387.8282 49
GCCGC 7465 99.57547 2363.7747 44
GTGAG 1825 99.40162 666.9929 16
CAGGG 795 98.85706 2284.1636 19
GGGCT 635 94.594315 2791.565 42
TGGGT 1390 90.69767 1551.7988 41
GGTTG 1375 89.71892 1222.0709 16
GGAGC 665 82.691765 672.19183 43
GAGGT 1475 80.338295 1176.9141 31
CCGCG 5780 77.09929 405.19266 45
GCTGG 515 76.71822 804.8241 13
GGCTG 490 72.99403 2147.3987 43
TGCGG 480 71.504364 860.76575 39
GCAGG 575 71.5004 1791.3531 18
CGCGC 5170 68.96252 299.05856 46
GCCCG 5025 67.028366 3212.4675 46
GGGAT 1170 63.72597 1255.375 31
GGCGA 505 62.795994 539.64636 44
AGAGC 34745 61.95743 2298.8103 8
CGCGA 12535 61.134476 115.38278 72
CGAGG 480 59.687286 628.6983 39
GGTGA 1095 59.640972 745.3769 30
TCGCG 10140 59.244835 147.90074 45
AGGTG 995 54.194313 1003.16986 39
TCGGT 20910 53.51267 2980.262 38
GTCGT 20835 53.32073 2036.1825 13
AGCGT 22990 49.11236 2223.5737 10
AAGAG 73705 48.05486 1011.85315 25
CGCGT 7795 45.543743 196.52356 48
CGTGT 17770 45.476814 2173.5356 15
GGAGT 830 45.207314 509.99612 31
TCGTG 16765 42.904823 2054.56 14
CGACG 8595 41.918694 175.66345 12
GGAAA 62330 40.638485 1502.5446 22
GGCCC 3040 40.55049 993.68677 45
ACGCG 8105 39.528908 80.41815 71
TGAGG 665 36.220318 882.784 17
GGATG 660 35.947987 431.5833 17
GAAGA 52610 34.301147 985.75085 6
TGGTC 13330 34.114006 2128.5872 42
GTCCG 5000 29.21343 101.063484 1
TGTAG 27650 25.872416 817.9429 17
GTGTA 27530 25.760132 771.43506 16
CCCGG 1805 24.076855 760.50793 37
CGGCC 1780 23.743382 1196.2832 45
TCCGG 4050 23.662878 1174.3346 37
CCGGA 4625 22.556593 795.41064 47
GCCGA 4530 22.093273 800.7017 47
GAAAG 33665 21.949215 974.95337 23
AGCGC 4185 20.41067 802.7492 10
GTAGA 24095 18.819931 954.6977 31
AAAGG 25835 16.84414 288.86584 25
CCGTG 2715 15.862893 532.4015 14
GACGC 3055 14.899545 229.29395 44
GACCG 2965 14.460606 275.93192 1
GCGCA 2840 13.85097 202.82092 47
GCTCG 2350 13.730312 82.22966 35
TTCGG 5355 13.704465 491.2905 37
GCGAC 2800 13.655887 147.56279 11
CCGGT 2245 13.11683 323.3129 48
GCGCT 2075 12.123575 65.497894 47
AGAGA 18445 12.02594 94.88589 72
TGGCC 2040 11.919078 526.3875 42
AGAGT 15190 11.864485 452.18704 26
GCGAA 6605 11.778065 22.371979 72
AGTGT 12235 11.448427 539.76215 28
AAGCG 6295 11.22527 391.54434 9
GAGAT 13780 10.763173 308.85623 31
GAACG 6005 10.708141 125.949844 1
GCGTA 4945 10.563751 117.43958 48
CGGAT 4835 10.328764 519.9792 48
TCGGC 1600 9.348297 132.83301 38
GGTCC 1580 9.231443 290.57147 43
GCGAT 4165 8.897477 20.860977 48
CCCCC 431765 8.859566 10.471656 6
GCACG 1730 8.4373865 33.216198 71
GCGTT 3235 8.278981 47.01198 11
GACGA 4550 8.113579 24.928774 72
GTTCG 3095 7.920694 159.54718 1
GGATC 3675 7.850714 352.71155 49
CCCCG 14515 7.593828 60.728245 46
GTCGA 3530 7.540958 26.267466 44
GTACG 3515 7.5089145 31.562843 1
ACCGG 1505 7.340037 258.3669 2
CCGGC 545 7.2697425 260.477 47
CTCTC 1820885 7.168487 8.88689 42
CGCCC 13155 6.882316 120.70471 45
TCGCC 29585 6.7796197 237.83482 45
GTGCC 1160 6.777515 259.89926 44
CGAGA 3770 6.72268 39.63029 71
GACGT 3140 6.7078214 45.396484 13
CAGAG 3580 6.383871 58.167034 71
CCGCC 12130 6.3460655 86.83927 45
CGGCA 1300 6.340233 76.92185 72
TCTCG 58095 5.831262 161.58676 36
CCCGC 11120 5.817663 32.918957 47
CTGTG 2190 5.6046267 94.20103 38
GGCTC 950 5.550551 166.39566 49
CGGCT 945 5.521339 264.14456 48
GTGTC 2155 5.515055 39.65347 41
GAGAA 8455 5.5125685 152.62183 31
GAGCA 3075 5.483353 170.09714 9
CGGTC 935 5.4629116 149.54549 49
AACGG 3020 5.385277 129.16286 1
TCGAG 2270 4.8492846 29.865547 61
TCTGG 1820 4.6577263 138.52174 37
TGCGC 785 4.5865083 57.05106 44
CGGTT 1740 4.4529915 80.26463 49
AGCGA 2495 4.4490943 52.022114 10
TGCCG 760 4.4404416 183.81197 46
TAGAG 5605 4.3779097 60.20345 19
CCCGA 22700 4.3421907 37.90676 47
CCGTC 18620 4.2669096 12.349207 48
CGCTG 725 4.2359476 33.8045 46
GGCCT 715 4.1775208 140.99605 12
CCGTA 48920 4.098826 117.6986 48
TGTCG 1565 4.0051327 44.269554 43
AGGAA 5955 3.8825953 85.271904 20
TCTCT 2212870 3.8158467 5.0246677 72
AGCCG 775 3.7797534 93.47159 46
CGAGC 770 3.7553687 22.727423 70
CTCGC 16230 3.7192235 9.610696 72
CTGCG 620 3.6224654 27.226408 71
ACGGA 2020 3.6020722 132.97238 3
CGTGA 1680 3.5888982 157.73428 15
CTCCG 15310 3.5083985 32.416756 36
AGAAG 5360 3.4946618 27.2648 25
CAGCG 700 3.413971 45.45374 71
ACGGC 695 3.3895855 105.39377 10
CGTCC 14230 3.2609093 6.112763 50
CGCAG 665 3.243273 51.14484 46
CCGAT 38705 3.242949 23.515478 48
CCTCG 14120 3.2357013 9.26193 36
CCGAC 16880 3.2289066 4.482278 49
TGTGT 2855 3.200359 15.670846 72
CGACC 16710 3.196388 4.2061515 50
CGCTC 13935 3.1933079 14.622089 49
GAGCC 645 3.1457303 26.456995 44
CCGAA 44300 3.0983183 9.460475 48
ACCGA 44190 3.0906248 3.7606833 60
GAGAC 1730 3.0849433 35.795097 71
TCCCG 13415 3.0741453 12.900546 36
CCGCT 13245 3.035189 25.029938 48
TACCG 35920 3.0096042 3.774339 2
GCCTG 515 3.0089834 117.84713 13
ACCCG 15630 2.9897988 5.049494 46
CCCGT 13025 2.9847744 29.086664 47
TCCGT 29500 2.9610505 7.352651 37
CGTGC 505 2.9505565 39.98403 15
CCTGG 505 2.9505565 94.695915 14
CACGG 600 2.9262605 58.07642 37
GAGCT 1340 2.8625734 47.690258 33
CTTGG 1115 2.853497 82.19442 36
CGGTA 1320 2.8198485 37.702343 15
CGAAA 110160 2.8169916 5.5354753 4
AAGGA 4310 2.8100731 61.546112 20
GCCCC 5355 2.8015814 29.32668 44
GGTTC 1090 2.7895174 81.18153 50
GAGTC 1290 2.755761 19.236567 11
GCAGA 1520 2.7104707 36.434364 72
ACCGC 13970 2.672264 5.0739937 72
CGCCT 11635 2.6662455 9.612442 46
GCTGC 450 2.6292088 76.068085 44
TTCCG 25975 2.6072302 9.598855 36
AGACG 1455 2.594562 21.820087 8
ACGTG 1205 2.5741796 51.550495 14
CGTTG 1005 2.5719864 38.81732 39
TAGCG 1200 2.5634987 20.675217 71
CCGCA 13385 2.5603623 19.924828 48
AAGTG 3275 2.558011 95.41899 27
GGCAC 520 2.5360928 50.97076 43
CACCG 13125 2.5106275 5.3261786 46
CGTAT 67895 2.4917295 52.272408 49
ATCCG 29645 2.483845 6.2285943 36
GGTCT 970 2.4824145 46.074303 33
TGGAA 3145 2.4564717 72.01946 4
ACGCC 12765 2.441765 11.206185 45
AGATC 79360 2.4311624 48.302925 33
CAGTG 1135 2.4246423 21.60148 39
AGTCG 1135 2.4246423 24.621891 12
AGGCC 490 2.3897796 38.647392 11
GGTAA 3035 2.3705537 47.256557 30
ACGAG 1305 2.3270814 25.567926 71
CTTCG 23155 2.3241737 7.2081966 36
GCAGC 475 2.3166234 44.094994 44
TTTCG 52475 2.3070996 7.5838814 36
CGTCT 22765 2.2850277 5.8619385 49
CGTAG 1065 2.2751052 23.852005 17
CCGTT 22530 2.2614396 15.174665 48
ACTCG 26585 2.227459 11.792041 35
CGATC 26545 2.2241077 12.655797 49
GGTCA 1025 2.189655 70.845406 50
CGTCA 25905 2.1704845 8.17828 12
AGGTC 1005 2.14693 38.492393 42
GACAG 1195 2.1309292 22.371979 72
ATAGG 2725 2.1284215 73.13834 18
AAGGC 1190 2.122013 60.96789 8
CGGAC 435 2.1215394 24.60611 42
ATTGG 2250 2.1053505 62.71845 1
CACGC 10945 2.0936244 7.748125 72
CCACG 10750 2.0563238 5.005418 71
CGTTT 46495 2.0441847 6.5458536 49
GTGAA 2585 2.0190713 51.481823 16
TTTGG 1795 2.0121348 34.78891 36
CACGA 28690 2.0065632 6.6937385 72
GGACC 410 1.9996115 47.455536 43
CTGAG 935 1.9973927 13.886666 39
ACACG 28240 1.9750905 7.8469534 71
ATCGA 64405 1.9730222 5.073289 72
CGCTT 19470 1.9542935 6.6941886 49
GAGTA 2475 1.9331534 45.56825 29
TGCGT 750 1.9193927 15.595047 72
GTTGT 1670 1.8720139 27.726494 60
GGATT 2000 1.8714225 70.50023 49
TGTGC 730 1.8682089 19.367561 42
GTCTG 720 1.842617 18.435331 13
AGTGA 2335 1.8238033 47.279144 28
GCTGT 710 1.8170253 12.843515 60
CATCG 21575 1.8076895 10.320929 12
TTGGA 1910 1.7872086 61.685493 3
TGAAG 2275 1.7769389 59.077446 17
CGATT 48360 1.7748001 5.8609667 49
TAGGA 2245 1.753507 59.07815 19
TGAGA 2200 1.7183585 17.918694 72
TAAGG 2170 1.6949266 54.295635 19
GTAAG 2165 1.691021 54.29115 18
AGCAG 925 1.649464 18.536749 71
AGATG 2095 1.636346 34.997444 72
AGTAG 2065 1.6129138 37.41144 30
CGCAT 19165 1.6057645 9.727408 48
TGTTG 1430 1.6029819 26.922924 59
ATGCG 740 1.5808243 22.972464 71
GTGTT 1400 1.569353 15.753148 41
AGGAC 880 1.5692197 43.667854 32
CCGAG 320 1.5606724 21.078964 14
ACGGT 725 1.5487803 26.981915 38
GGAAC 860 1.5335556 28.897846 32
GTGCA 710 1.5167366 11.541302 16
GGCAT 705 1.5060554 44.627174 12
AGTGC 705 1.5060554 12.25198 71
TACGG 700 1.4953742 21.584105 37
GTGAC 695 1.4846929 6.928631 42
TTGCG 575 1.4715345 12.842959 71
TGCGA 685 1.4633304 19.909502 72
GATCT 39400 1.44597 56.93432 34
TTGTG 1285 1.4404418 11.304643 14
GGACT 660 1.4099241 64.61261 33
CTGGT 535 1.3691667 25.809582 15
GTGCT 535 1.3691667 12.910431 41
CGAGT 640 1.3671993 19.23589 15
TGGAC 640 1.3671992 68.51646 42
GTAGC 640 1.3671992 28.585184 44
GGCCA 280 1.3655883 42.159225 12
GATAG 1740 1.3590654 15.480831 1
TGTGA 1420 1.32871 8.04986 72
GGACA 745 1.3284872 13.495275 43
GCATG 615 1.3137931 40.779896 13
TCAGG 615 1.3137931 19.27152 37
CTGGA 615 1.313793 23.8525 15
GTTGC 510 1.305187 25.914808 44
TGCAG 605 1.2924305 15.388388 17
GGTTT 1150 1.2891114 15.356017 49
AAGGT 1650 1.2887689 27.58425 27
CGAAG 715 1.2749912 8.948776 71
GGCTT 490 1.2540032 40.5936 49
TGACG 585 1.2497056 11.486769 59
TTGGT 1100 1.233063 26.294134 38
GATTG 1300 1.2164246 52.602566 1
GATGT 1270 1.1883533 13.081565 60
GCAGT 550 1.1749369 16.22274 47
CAGGA 655 1.1679987 7.7073073 15
CGATG 545 1.1642556 10.800741 39
GTCAG 525 1.1215307 7.6982546 1
GGTAC 525 1.1215307 20.016424 43
AATGG 1390 1.0856901 25.050798 1
ATGGA 1370 1.0700688 19.414682 3
ATGTG 1135 1.0620323 9.391504 72
GTATC 28900 1.0606227 47.029972 50
AGGTA 1350 1.0544473 23.076725 28
GGAAT 1345 1.0505419 11.53259 32
GCCAG 215 1.048577 24.592817 13
CCAGG 215 1.0485768 24.638483 37
TTAGG 1115 1.0433182 36.732376 18
GAAGC 580 1.0342585 10.268221 6
TGAGC 480 1.0253994 5.360376 69
GGCAA 575 1.0253423 16.070276 22
TGCTG 400 1.0236762 14.678354 59
ACAGG 570 1.0164264 7.0781193 37
GTAGT 1085 1.0152467 20.21883 31
GCCCT 4365 1.0002718 12.0155525 46
GAAGT 1280 0.99977225 19.690287 31
CTAGG 465 0.99335575 30.063574 37
GGATA 1230 0.96071863 11.5344095 15
GGTTA 1025 0.95910406 13.154626 50
GGTAT 1010 0.9450683 11.468935 49
TAGTG 995 0.9310328 7.379026 71
GAAAA 99165 0.9271698 8.915204 23
GTTTG 825 0.9247973 19.389845 1
CTGGC 155 0.9056162 14.737394 41
CAGGC 185 0.90226376 7.0296216 41
GTGAT 960 0.89828277 8.088441 16
GACTG 415 0.88654333 5.388779 1
ACTGG 415 0.8865432 16.188076 37
ATGAG 1105 0.86308473 10.639205 71
GAGTT 910 0.85149723 11.792234 33
CATGG 390 0.83313704 14.646355 37
GTCCC 3600 0.8249664 11.270939 44
GCAAG 460 0.82027406 12.214044 23
TATGG 855 0.80003315 12.468788 18
AGGAT 1000 0.7810721 14.626699 32
GATGC 360 0.7690496 10.816015 44
GCTCC 3340 0.7653856 5.8649483 50
TGGTA 810 0.7579261 13.151023 42
TGGTT 670 0.7510475 12.119005 42
GTATG 780 0.72985476 7.7555065 1
TTGGC 285 0.7293693 16.599127 41
GTTAG 770 0.72049767 13.143705 17
GAATG 920 0.7185863 14.636422 1
AGTTG 760 0.7111406 12.41076 59
TGGCA 330 0.7049621 18.476349 42
GATGA 870 0.6795327 7.2797713 60
TGATG 715 0.6690335 9.436506 17
TGGCT 260 0.6653895 20.289825 42
GCTGA 310 0.66223717 9.955165 60
CAGGT 310 0.6622371 11.563678 38
GCTTG 255 0.65259355 7.377905 1
GTTGA 690 0.6456408 11.069016 60
AGGTT 660 0.61756945 8.7656555 28
AGGCA 345 0.61520547 5.776884 9
CAAGG 340 0.60628945 5.1424794 26
GATCA 19195 0.5880313 7.509193 50
GCATC 6960 0.5831528 8.993424 11
TAGAT 43325 0.5813544 16.019089 32
AAAGA 61855 0.5783299 13.286857 24
TAGGT 595 0.5567482 14.182217 38
AAAAG 57880 0.5411646 5.1569896 25
TGGAT 565 0.5286768 6.406908 42
AGGCT 245 0.52338094 6.1516533 9
ATGGT 550 0.5146412 6.0780926 38
TAGGC 220 0.46997476 5.3963833 38
CACAG 5805 0.40599862 5.665851 71
TCAGA 12375 0.3791033 7.3683476 72
ACAGA 13730 0.35110104 5.7564726 72
ATCAG 8520 0.2610069 6.1384487 71
TGCCC 1105 0.25321886 8.03832 45
AAAGC 9165 0.23436572 6.2857747 8
TTTGT 8710 0.16773435 5.446482 72
Produced by FastQC (version 0.10.1)