FastQCFastQC Report
Mon 3 Feb 2014
filtered_BS_CgM3_GATCAG_L007_R2.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename filtered_BS_CgM3_GATCAG_L007_R2.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5526741
Filtered Sequences 0
Sequence length 76
%GC 17

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 58175 1.681362E7 3.09511744E8 40
CGGGG 21470 229765.0 8468409.0 39
GGGCG 20080 214889.67 5619257.0 43
GGGGC 18570 198730.16 3817715.8 41
GGCGG 11110 118895.63 3126236.0 12
GGTGG 24270 113731.555 5660029.5 40
GAGGG 28675 113286.77 3774461.2 27
GGGGA 28490 112555.9 2492524.2 20
AGGGG 27785 109770.64 1354022.1 27
GCGGG 7775 83205.54 3091540.0 13
GGGAG 18190 71863.52 1151071.5 30
GTGGG 10670 50000.65 1183711.2 41
GGGGT 9520 44611.633 1430247.2 41
TGGGG 7255 33997.63 1097153.2 19
GGAGG 8300 32790.94 967651.0 17
GGGTG 6115 28655.477 815244.0 16
GGCGC 12005 4757.0864 188033.88 44
TCGGG 24575 4264.1416 115907.36 38
GAGCG 25865 3783.688 164186.05 9
CGGTG 19105 3315.013 203294.98 39
GGTCG 18795 3261.2231 179155.58 43
GGCCG 7630 3023.4543 73650.83 12
GGAAG 55785 3012.619 84225.19 5
GGGAA 50405 2722.077 122688.695 21
CCGGG 6740 2670.7837 110328.375 14
GCGGC 6595 2613.3267 66231.06 11
GGGCC 6520 2583.6072 74081.73 11
GCCGG 6280 2488.5051 110333.41 13
CGGCG 6245 2474.636 67513.26 12
CGGGT 10915 1893.9211 110187.33 15
AGGGA 30595 1652.2555 84409.96 20
TAGGG 24940 1597.5651 88877.36 19
GTAGG 24185 1549.2025 86377.22 18
GGGTA 21550 1380.4141 49921.89 29
GTCGG 7915 1373.375 74438.67 13
GGTAG 20450 1309.9523 46208.414 30
GGGTC 7375 1279.6765 45960.83 42
AGAGG 23480 1268.0162 52175.344 26
AAGGG 22765 1229.4033 16815.506 27
CGGGA 7625 1115.4309 24347.982 3
GTGGT 13945 1059.5369 63956.83 41
AGGGT 14610 935.8631 46231.863 28
TGGGC 5055 877.1207 39957.79 42
GCGCG 2190 867.8067 5015.9136 45
GGCGT 4920 853.69604 19937.812 13
GGAGA 14480 781.97943 19079.936 31
GAGTG 11390 729.6017 34373.7 27
AGCGG 4055 593.18976 24079.043 10
AGGGC 4015 587.3384 18810.107 10
CGTGG 3250 563.9253 12874.981 15
GCGTG 3225 559.5874 18749.443 14
CGCGG 1340 530.98676 10174.675 46
GAGAG 9795 528.9702 4547.952 6
GTGGC 2980 517.0761 22446.53 41
CGCCG 32925 483.09402 23326.355 46
GAAGG 7695 415.56155 12322.229 6
CTGGG 1470 255.06769 5385.215 38
TGGGA 3900 249.81973 8353.893 3
GTGGA 3635 232.8448 4267.8145 29
GGTGC 1340 232.51073 5627.924 43
TTGGG 3010 228.69882 4023.069 19
GCGGT 1230 213.42401 3939.0837 41
ACGGG 1440 210.65187 5482.766 2
GCGCC 14050 206.14946 6903.77 45
GGCAG 1390 203.33757 2097.1377 72
CGGGC 505 200.11069 2856.0857 42
GATCG 82870 196.55608 4545.4795 1
GACGG 1310 191.63467 5166.198 1
TGGAG 2985 191.20819 4060.7744 17
GGACG 1290 188.70897 6695.362 43
TGGCG 1085 188.26428 4502.2856 42
CGGAG 1240 181.39467 4913.528 39
CGTCG 27605 177.3589 6942.815 12
GCGGA 1210 177.00609 5184.682 47
CTCGG 27535 176.90915 10483.904 37
ATCGG 74300 176.22923 4314.913 2
AGGCG 1185 173.34894 3686.0242 9
GTGTG 2110 160.31714 2326.1929 16
GCCGT 24810 159.40134 9138.657 47
GTTGG 2020 153.47897 3338.5554 18
ATGGG 2345 150.21211 5978.9565 2
GATGG 2330 149.25127 6532.6704 1
TGGTG 1945 147.78049 3457.6072 39
TCGGA 62300 147.76689 4398.063 3
TGTGG 1925 146.2609 2846.1963 17
GGGAC 980 143.3603 3215.8442 42
GCGTC 22280 143.1464 6917.6084 11
GGGCA 925 135.31458 1686.9973 21
GGTGT 1735 131.82477 2655.6616 40
GCGAG 880 128.7317 576.7102 71
GTGCG 730 126.66629 1625.8446 43
CGGAA 59400 118.779144 3956.4934 4
AGTGG 1830 117.2231 4293.1235 28
GTCGC 17660 113.463425 6585.4946 44
GAGGA 2095 113.138596 1342.6852 28
CAGGG 770 112.640236 2950.758 19
GGGCT 625 108.44716 1938.4839 42
AGGAG 2000 108.00821 1244.7393 29
GGGTT 1330 101.052986 1122.0459 16
GGTTG 1320 100.29319 1259.5714 43
GAGGC 685 100.205925 1370.5431 41
TGGGT 1300 98.773605 1533.2117 41
GCCGC 6575 96.47208 2775.4343 44
GGCTG 550 95.43351 2126.1045 43
GGAGC 600 87.771614 846.5374 44
GAGGT 1365 87.436905 1500.348 41
GCAGG 595 87.04019 2212.8577 18
CCGCG 5815 85.32093 366.1492 45
CGAGG 520 76.06874 1003.8391 39
AGGTG 1145 73.344505 1249.2906 39
GGCGA 480 70.21729 634.9031 44
GCTGG 395 68.53861 875.0138 13
CGCGA 12530 67.87048 165.011 72
AGAGC 32115 64.21873 2381.3706 8
CGCGC 4300 63.092007 164.49432 46
GGGAT 980 62.775215 830.4921 32
GTGAG 975 62.454933 876.7486 16
GGTGA 965 61.814373 692.0881 30
TCGCG 9445 60.68301 139.41823 45
TGCGG 340 58.99526 502.00897 46
GTCGT 18995 53.43967 2219.3064 13
TCGGT 18750 52.750393 3176.8484 38
AAGAG 70365 51.943886 1167.0172 25
CGACG 9410 50.97057 175.59877 12
AGCGT 20885 49.5363 2469.7751 10
CGTGT 17380 48.89611 2441.1953 15
CGCGT 7375 47.38351 124.968376 15
GCCCG 3170 46.512012 1889.0315 46
ACGCG 8410 45.55393 112.59522 71
TCGTG 16135 45.39348 2233.3923 14
TGAGG 680 43.55831 1061.3386 17
GGAAA 55555 41.011047 1603.7021 22
GGAGT 635 40.675774 300.0606 40
GGATG 620 39.71493 738.3225 17
GAAGA 49760 36.733143 1005.315 6
TGGTC 12600 35.448273 2257.9124 42
GTCCG 5415 34.79074 127.34154 1
GTGTA 26935 27.9747 869.38855 16
TGTAG 26460 27.481365 885.4842 17
GGCCC 1820 26.704058 518.20245 43
GAAAG 33240 24.537977 1123.648 23
GCCGA 4510 24.42904 842.348 47
CGGCC 1550 22.742468 971.09143 45
CCCGG 1405 20.614946 836.5469 37
GTAGA 22810 19.972775 1096.1602 31
AGCGC 3535 19.147816 920.8566 10
TCCGG 2765 17.764801 788.2635 37
GACGC 3130 16.954077 240.96739 44
GACCG 3130 16.954077 298.65063 1
CCGTG 2490 15.997956 597.05426 14
CCGGA 2940 15.924916 362.40552 47
GCTCG 2275 14.61661 67.14373 1
GCGAC 2675 14.489507 144.38643 11
AAAGG 19255 14.214161 238.44571 26
TCGGC 2165 13.909869 177.30379 71
GCGAA 6780 13.55762 27.36949 16
GAACG 6385 12.767758 146.28238 1
TTCGG 4480 12.603829 480.1898 37
AAGCG 6275 12.547797 556.4045 9
GCGCA 2310 12.512436 78.357956 47
TGGCC 1925 12.367899 606.63696 42
AGTGT 11450 11.8919735 546.3861 28
AGAGT 13485 11.807665 480.43158 26
GCGCT 1780 11.436291 37.17735 47
GCGTA 4615 10.946136 130.407 48
GCGAT 4290 10.175282 20.40152 71
CCGGT 1565 10.05494 299.1034 38
GACGA 4805 9.608313 21.695427 47
GTCGA 3945 9.35699 39.30028 13
GTTCG 3290 9.255937 165.25488 1
GCGTT 3250 9.143403 49.657566 11
GCACG 1670 9.045787 31.061628 70
CGGCA 1630 8.829122 149.48056 72
CCCCC 436180 8.774567 10.575299 20
GTACG 3660 8.681009 23.077839 1
GGTCC 1320 8.480845 333.4227 43
CCCCG 15400 8.36669 59.336674 46
GAGAT 9415 8.243914 273.40286 32
GACGT 3465 8.218496 50.406876 13
CCGGC 560 8.216633 153.88527 47
AGAGA 10225 7.5481586 75.402664 72
ACCGG 1350 7.3124614 259.6241 2
CCGCC 13160 7.149716 100.60186 45
TCGCC 29175 6.9406967 244.6949 45
CGCCC 12510 6.796576 69.94973 45
CTCTC 1757665 6.779764 9.24728 70
CCCGC 11875 6.4515862 29.07956 47
GTGCC 960 6.167887 185.89964 44
TGCCG 895 5.75027 237.00018 46
CGGAT 2415 5.728043 223.0646 48
TCTCG 54690 5.697173 165.63744 36
CCGTC 21140 5.02918 13.940404 48
AACGG 2455 4.909139 139.80383 2
CAGAG 2400 4.7991576 60.19982 71
CGGTC 745 4.7865376 131.97827 42
GAGCA 2365 4.72917 106.53022 9
CCCGA 23570 4.727336 23.646545 47
GGCTC 715 4.593791 88.0201 49
GGATC 1930 4.5776906 144.51367 49
TGCGC 705 4.5295424 55.769894 44
CCGTA 51180 4.4948626 124.40189 48
TCTGG 1565 4.4029 98.47444 37
CGAGA 2165 4.329241 53.750088 72
GTGTC 1510 4.248166 46.63359 41
ACGGC 750 4.0624785 97.548386 10
AGAAG 5485 4.049062 38.891727 71
CGGCT 625 4.0155516 132.46716 48
ACGGA 1985 3.9693031 142.63863 3
CTCCG 16515 3.9288983 20.17542 36
GAGAA 5260 3.8829648 69.656265 31
CTCGC 16245 3.8646657 10.913557 72
GGCCT 600 3.8549297 87.94231 12
CGTCC 16090 3.8277917 5.4202104 46
CCTCG 16060 3.8206544 10.130636 36
CCGAC 18955 3.8017242 5.94893 48
AGCCG 695 3.7645636 95.98629 46
CGACC 18670 3.7445636 5.440032 46
TCGAG 1570 3.7238207 39.10291 71
CTGTG 1310 3.6854947 60.96539 39
ACCGA 49245 3.646221 4.537677 26
TCTCT 2155560 3.6408095 5.3833203 72
AGGAA 4920 3.6319747 72.09557 21
TGTCG 1290 3.6292276 55.764095 43
CCGAT 41255 3.6232033 16.836824 48
CGCTG 560 3.5979342 37.12725 39
CAGCG 660 3.5749812 27.17892 70
TCCCG 14990 3.5661027 14.251574 36
CCGAA 48000 3.5540373 10.1505165 48
CTGCG 550 3.533685 25.329113 71
TACCG 40130 3.5244012 4.817506 36
TCCGT 33825 3.523622 6.503167 37
CGTGA 1485 3.5222132 164.03313 15
ACCCG 17445 3.4988704 5.8752346 46
CGTGC 540 3.469437 50.913044 15
CGCTC 14555 3.4626167 6.775672 49
CGCAG 635 3.4395652 43.09589 46
CGGTT 1220 3.4322927 46.65685 49
CCGCT 14305 3.4031417 17.03827 48
CCCGT 14225 3.3841102 28.392223 47
TCCGC 14090 3.3519938 5.7648816 44
CGAAA 121800 3.3292935 8.309872 4
AGCGA 1580 3.159445 59.059235 10
AGATC 96345 3.1237 50.889523 33
CCTGG 485 3.1160681 74.053246 14
ACCGC 15470 3.102753 6.181864 72
GCAGA 1550 3.0994563 35.116726 72
TTCCG 29340 3.0564098 10.187867 36
GCTGC 475 3.0518196 76.68361 44
CTCGA 33640 2.9544194 5.602738 72
CGCCT 12340 2.935671 10.754384 46
GAGCC 535 2.8979015 38.995743 9
ACGCC 14445 2.897173 11.9686165 45
CACCG 14265 2.861071 6.0928354 46
AAGTG 3235 2.832614 106.34387 27
CGTAT 72600 2.7919788 56.290436 49
CCGCA 13820 2.771819 22.12712 48
TAGAG 3145 2.7538087 40.05526 19
CACGG 505 2.7354023 68.41087 37
TGGAA 3120 2.7319183 71.91095 4
TTTCG 59355 2.7074978 8.016823 36
GGCAC 495 2.6812358 44.897808 43
CGTCT 25610 2.6678483 6.7226057 49
CGGTA 1120 2.6564837 29.169987 48
CGGAC 490 2.6541526 23.42467 42
GGTTC 935 2.630487 44.623943 50
CTTGG 935 2.6304867 98.47444 37
CTTCG 25190 2.6240957 6.202945 36
CGTTG 930 2.61642 42.675777 39
AAGGA 3525 2.602177 63.57012 20
GCCTG 405 2.6020775 90.25649 13
GCAGC 480 2.5999866 50.936203 44
CGCCA 12875 2.5822847 5.8027005 46
GCCCC 4750 2.5806344 20.6322 44
CCGTT 24685 2.5714886 15.675196 48
ACGTG 1070 2.5378907 57.239113 14
ACGTC 28590 2.5109053 6.327192 11
CCACG 12490 2.5050664 6.6850076 71
CGATC 28315 2.4867537 6.7758384 49
CACGC 12245 2.455928 6.972567 72
CGTCA 27840 2.4450371 5.3779182 12
TCGAA 75075 2.4340832 7.6740327 3
GGTAA 2770 2.425453 61.177654 30
GGTCT 860 2.419485 38.52792 43
ACTCG 27235 2.3919032 8.050307 36
GAGAC 1185 2.369584 25.08326 71
TGTGT 1920 2.3652937 17.749453 15
CGCAC 11750 2.356648 5.750706 70
CGTTT 51545 2.3512423 6.759051 49
CACGA 31520 2.3338184 7.562925 72
CTCGT 22355 2.3287678 6.3096075 72
ACACG 31360 2.3219717 7.7486463 71
ATCGA 71605 2.321579 6.776909 2
CGTAG 970 2.300705 32.46436 17
CGCTT 22065 2.298558 6.2343717 49
AGACG 1135 2.2696016 21.500034 72
AAGGC 1135 2.2696016 59.732174 8
CGAGC 415 2.247905 17.471674 71
GTGAA 2515 2.2021713 57.707977 29
CCGAG 405 2.1937385 25.363085 14
ACGAG 1070 2.1396244 23.649931 71
GGACC 395 2.1395721 40.993652 43
AGGTC 890 2.110956 47.012592 42
GACAG 1050 2.0996313 23.650036 72
GAGTC 880 2.0872374 29.903502 11
CGATT 53290 2.049374 5.9756594 49
TGCGT 725 2.0396821 18.149351 72
TGAAG 2315 2.0270483 58.347046 17
GTCTG 705 1.983415 28.374695 13
TTGGA 1905 1.9785335 61.737717 3
AGTCG 830 1.9686443 40.15467 12
CATCG 22250 1.9540975 7.5607204 12
GCTGT 690 1.9412149 23.19189 60
ATTGG 1850 1.9214107 50.529594 2
GAGTA 2170 1.9000841 52.66247 29
CACGT 21425 1.881642 5.5083094 72
CTGGT 655 1.8427471 43.65755 38
GAGCT 770 1.8263326 21.344524 9
TTTGG 1480 1.823247 26.227875 37
CGAAG 910 1.8196808 20.888098 4
AGGCC 335 1.8145738 37.089058 42
ATAGG 2065 1.8081446 52.66606 18
GTAAG 2045 1.7906324 61.4963 18
GTGTT 1450 1.7862896 30.616974 16
AGTGA 2030 1.7774981 56.79158 28
TAGGA 2020 1.768742 53.61728 19
GTTGT 1435 1.7678107 18.986113 60
TTGCG 625 1.7583467 9.074635 71
CAGTG 740 1.7551768 17.132738 39
GTGCA 740 1.7551768 17.086315 16
TGTTG 1415 1.7431718 32.39222 17
TAAGG 1990 1.7424735 63.394547 19
CGCAT 19730 1.7327793 9.5305 49
TGGAC 730 1.7314582 73.5106 42
ACGGT 730 1.7314582 28.246817 38
TCAGG 720 1.7077394 17.939474 18
TAGCG 710 1.684021 11.051153 69
AGTAG 1900 1.6636682 40.36464 30
AGCAG 810 1.6197156 12.8999605 71
AGATG 1845 1.6155094 44.562088 72
TACGG 680 1.6128651 19.685402 37
TGTGC 560 1.5754788 14.19433 42
ATGCG 655 1.5535686 13.601074 72
GTTGC 545 1.5332785 40.701263 44
CGAGT 630 1.4942722 29.047533 15
TGCAG 630 1.4942721 22.212456 17
GGCCA 275 1.4895756 33.16865 12
GGTCA 625 1.4824128 23.079182 43
GTGAC 620 1.4705535 7.6921573 41
GAAGC 730 1.4597437 13.617008 70
TGCGA 615 1.4586942 11.90094 72
TGACG 610 1.4468349 16.152037 59
CAGGA 720 1.4397472 12.183639 70
CTGGA 605 1.4349755 30.756205 15
TTGTG 1130 1.3920736 16.41778 14
CTGAG 585 1.3875383 9.423004 39
GATCT 35975 1.3834908 59.331635 34
GTAGC 570 1.3519605 26.59342 44
TTGGT 1090 1.3427967 36.45559 38
TGAGA 1530 1.3396907 15.690876 72
GTGCT 475 1.3363435 11.15151 41
CGATG 560 1.328242 14.562828 39
GGAAC 655 1.3097701 10.092498 22
GCAGT 550 1.3045233 15.440272 47
CCAGG 240 1.2999932 37.137333 37
AAGGT 1470 1.2871537 21.14255 27
GGACA 640 1.2797754 17.295475 43
GATAG 1450 1.2696415 20.825682 1
AGTGC 535 1.2689453 11.965078 28
TGCTG 440 1.237876 19.15855 59
GATTG 1190 1.2359344 58.386875 1
TTAGG 1190 1.2359343 43.3918 18
AGGAC 605 1.2097876 20.177816 42
CTGGC 185 1.1886032 9.274524 38
ATGGA 1345 1.177702 19.248924 2
GGACT 495 1.174071 19.643478 33
GGTTT 925 1.1395296 10.6592865 49
ACTGG 480 1.1384932 15.405969 37
GTATC 29495 1.1342894 49.893974 50
GGCAA 565 1.1298016 21.626781 22
GGCAT 475 1.1266338 17.087093 12
CTAGG 475 1.1266336 45.362007 37
GTAGT 1060 1.1009163 19.450598 31
ATGTG 1030 1.0697583 9.677963 71
GATGT 1030 1.0697583 17.867897 60
GCCCT 4410 1.0491337 12.647445 47
GACTG 435 1.0317593 11.966284 1
GTCAG 435 1.0317593 5.128707 43
GCCAG 190 1.0291612 11.706573 13
GGATT 990 1.0282143 33.325104 49
GAAGT 1145 1.0025789 17.975008 31
GGTTA 955 0.99186337 11.596835 16
GAAAA 97715 0.9860297 7.723479 23
GGATA 1120 0.98068863 7.5760217 22
GCATG 410 0.9724629 12.81531 13
GCTTG 345 0.97060746 11.15217 1
TGTGA 925 0.96070534 8.189083 72
GCAAG 480 0.95983166 8.651059 23
TGGTA 915 0.95031935 13.848813 42
ACAGG 475 0.9498334 6.4947553 38
TGGCA 400 0.9487442 20.514585 42
GATGC 400 0.9487442 18.87275 44
GGAAT 1070 0.9369079 7.260354 22
CATGG 395 0.936885 19.685404 37
GTGAT 900 0.9347403 8.978195 16
GGTAC 390 0.9250256 19.660044 43
TGGTT 750 0.92394286 19.090382 42
AATGG 1045 0.9150175 12.622246 2
CAGGT 380 0.901307 13.695427 38
ATGAG 1025 0.89750516 8.473035 71
AGGTA 1020 0.89312714 17.668491 28
GGTAT 850 0.8828103 11.233182 49
AGCTG 370 0.87758833 10.201285 59
GTCCC 3630 0.8635726 11.44372 44
GTTTG 700 0.86234653 18.645554 1
GTTAG 825 0.8568453 15.712013 17
AGGTT 820 0.8516522 11.596592 10
TAGTG 805 0.8360732 6.700128 71
GCTGA 350 0.83015126 10.201269 60
GGCTT 295 0.8299396 17.242748 49
TTGGC 295 0.8299396 14.192831 41
CAGGC 150 0.8124957 7.819092 38
TGATG 765 0.7945292 10.474674 17
GTATG 750 0.7789502 7.485497 1
TAGAT 54355 0.771683 17.240816 32
TGGCT 265 0.745539 24.333136 42
TATGG 695 0.72182727 11.970155 18
GAATG 815 0.7136261 14.514869 1
AGGCT 300 0.71155816 6.8343835 10
GATCA 21510 0.6973977 6.681628 70
TAGGT 670 0.6958622 15.742156 38
GTTGA 650 0.67509025 11.539684 60
GCTAG 275 0.6522617 5.128409 1
GGCTA 270 0.6404023 5.979504 30
AAAGA 63460 0.64036673 14.577474 24
GATGA 715 0.6260646 5.3351393 60
TGGAT 600 0.62316024 6.7372603 42
GAGTT 600 0.6231602 7.860265 27
AGTTG 570 0.59200215 10.422959 59
AGGAT 670 0.58666193 8.5142765 32
ATGGT 535 0.5556511 6.7466383 38
GCATC 6045 0.53089976 5.983686 50
TCAGA 16225 0.5260473 10.364994 72
AAAAG 52125 0.52598673 5.520163 7
ATGGC 220 0.52180934 5.983421 42
CAAGG 260 0.5199088 6.4818296 18
ATCAG 12660 0.41046283 8.761402 71
CACAG 5435 0.40242076 6.713724 71
ACAGA 14175 0.3874609 6.0346155 72
AAAGC 8730 0.23862666 8.430668 8
TCTGT 4550 0.20754974 5.1006966 72
TGCCC 800 0.19031903 5.3344183 45
TTTGT 8315 0.16608575 5.927404 72
Produced by FastQC (version 0.10.1)