FastQCFastQC Report
Mon 3 Feb 2014
filtered_Bs_CgLarve_T3D3_GCCAAT_L007_R1.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename filtered_Bs_CgLarve_T3D3_GCCAAT_L007_R1.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5837666
Filtered Sequences 0
Sequence length 76
%GC 17

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC 42626 0.7301890858435546 TruSeq Adapter, Index 6 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC 20787 0.3560840925123157 TruSeq Adapter, Index 6 (100% over 50bp)

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CCCCC 2020 205048.28 437974.47 15
CACGC 83030 7567.283 350975.56 31
ACGCC 83010 7565.4604 351407.6 32
CGCCA 82930 7558.169 351730.75 33
CCGTC 96445 7232.9795 351213.06 50
CACAC 103540 4243.1177 190592.64 12
ACTCC 96060 3239.3074 154771.52 23
CTCCA 95720 3227.8416 154466.08 24
ACCCC 390 795.43634 10273.899 31
CACCC 385 785.23846 9539.297 30
CCCCA 365 744.44684 11007.928 32
CCGCC 115 521.63495 9805.915 49
CTCCC 240 402.7956 9658.6875 24
CCCAC 180 367.12448 2939.6152 54
CGCCC 80 362.8765 11424.585 32
CCACC 175 356.92657 2938.46 55
TGCCG 97900 328.08344 15938.422 48
GCCGT 96945 324.88306 15824.858 49
CCGGC 1310 265.52426 9932.071 49
CGTCT 194195 240.79327 5806.452 51
CCCGC 50 226.7978 8159.6406 30
CGCGC 1095 221.94585 1969.0789 31
AGCAC 104540 191.43594 8668.773 10
GCACA 102415 187.5446 8647.046 11
ACACG 99725 182.6186 8583.837 13
GCCCC 40 181.43825 3271.6147 65
CCCCT 95 159.43993 3026.212 67
ACGTC 102950 155.1315 7015.185 15
GCCAA 83275 152.49501 7100.8394 34
CACGT 100475 151.40202 7034.148 14
ATGCC 98285 148.10199 7163.8174 47
GTCAC 97505 146.92662 7018.357 29
TCCAG 97020 146.1958 6997.486 25
CAGTC 96875 145.97731 6988.723 27
CCAGT 96775 145.82663 6980.326 26
TCACG 87725 132.18951 6129.8975 30
CCCTC 75 125.87363 4232.915 49
TCTGC 97835 121.31109 5886.237 56
CTGCT 97725 121.1747 5887.0005 57
TCTCG 97475 120.864716 5851.2944 41
CTCGT 97215 120.54232 5837.4756 42
CACCA 2825 115.76983 6826.913 31
CCTCC 55 92.30733 2414.5166 23
TCACC 2710 91.385826 5702.163 30
CCCCG 15 68.039345 1635.7047 70
TCCCC 40 67.1326 1210.5056 65
AACTC 97535 66.085495 3153.6018 22
CCAAT 85510 57.937874 2626.641 35
ATCTC 97460 54.33813 2629.6023 40
CGGCG 5720 51.807545 84.936226 72
CGCGG 5675 51.399967 81.41412 9
TCGCG 14520 48.65957 85.60363 30
GCGCG 5070 45.920322 81.44447 24
CTTCT 97440 44.70419 2164.5764 54
TCTTC 96625 44.330276 2148.0305 53
CGTCG 12800 42.895493 91.770966 50
CGCGA 10005 40.746105 66.04027 47
CGCCG 190 38.51115 1093.9506 32
CGCAC 415 37.822742 1081.3883 12
CGACG 9155 37.284412 61.50158 19
GCGCC 175 35.4708 1823.2509 32
CGCGT 10465 35.07041 67.476494 14
ACGCG 7645 31.134827 73.21529 13
CGGAC 6300 25.657217 39.53626 3
CTCCG 300 22.498772 944.12665 24
CCACG 225 20.506308 327.69342 12
CGTCC 270 20.248896 539.30316 16
CCCAG 220 20.050611 458.96024 25
ACACC 455 18.646112 176.81575 12
CCCAA 430 17.621603 235.9419 33
GCCAC 190 17.316437 458.77078 11
CACCG 180 16.405046 327.69342 14
GCACC 170 15.493654 426.00143 11
GCCGC 75 15.201771 438.2146 48
CGGAA 171390 14.024603 395.4572 4
ATCGG 202360 13.625811 330.1319 2
AACCC 325 13.318651 191.58337 21
GCTCC 175 13.124284 593.4129 23
TCGGA 187080 12.596939 326.3793 3
GCCGG 1360 12.317878 440.58878 48
GCGGC 1360 12.317878 32.575695 23
CCGCG 60 12.161416 73.10642 72
CTTCC 435 12.070672 260.1785 66
ACCCA 285 11.679434 103.173546 22
CCAAC 285 11.679433 147.66125 54
CGGGC 1270 11.502724 26.073246 33
TCGGC 3425 11.477894 56.63205 3
GATCG 168390 11.338459 328.37793 1
ACCAC 275 11.269629 132.61182 13
GCGTC 3355 11.24331 55.427116 15
CCACA 270 11.064727 88.40788 12
CCAGC 120 10.936697 295.0537 26
CAACC 255 10.450019 132.86913 43
CGATC 6915 10.419954 251.05148 33
AGAGC 124130 10.157384 395.2218 8
CGGCC 50 10.134514 218.80756 33
CGGTC 2920 9.785533 37.373802 27
TTCGC 7890 9.783253 16.528673 45
CCGAC 105 9.56961 361.2332 50
GAGCA 112465 9.202852 393.5742 9
GCCCA 100 9.1139145 229.56786 33
GGGGG 503925 9.113624 10.186433 62
ACGGC 2230 9.08184 111.28724 15
CGCCT 120 8.999509 162.13576 50
CAGCC 95 8.658218 196.72523 27
CGCTC 115 8.624529 134.82497 2
TCCCG 110 8.24955 134.90678 29
GCCCG 40 8.107611 146.07153 48
CGAGC 1975 8.043333 29.286116 7
CCCGT 105 7.8745704 80.93055 26
TCGTC 6330 7.8489213 27.700592 52
GTCGC 2255 7.556978 44.60973 29
CGTTC 6045 7.4955344 11.61626 53
ACGAC 4090 7.489698 12.524763 39
AGCGC 1810 7.3713584 55.64362 10
GGCGC 810 7.336383 22.83568 55
CGGCT 2130 7.1380773 164.22171 50
ATCGC 4715 7.104856 17.363224 40
TACGC 4705 7.0897875 88.37464 31
TGCGC 2080 6.9705167 15.682196 37
CGGGG 16120 6.5241823 9.02729 1
CGTAC 4240 6.389097 11.400446 44
TCGAC 4150 6.253479 14.086885 18
GAGAG 1661795 6.0764036 6.8469787 42
TCCGC 80 5.999672 81.03594 55
AGCCC 65 5.924044 131.36879 47
TCCCA 175 5.9012985 157.68669 25
GACGC 1415 5.762692 29.304287 30
CACTC 170 5.7326903 109.19897 31
AACGC 3100 5.676788 15.806859 22
GTCTG 100040 5.5429893 261.51935 17
GCTTG 97830 5.4205384 262.15115 59
CACGA 2810 5.145734 299.1323 31
GGCGG 12570 5.087405 7.7157664 25
CCCGG 25 5.067257 73.09336 68
GCGAC 1230 5.0092664 11.739826 48
CGTGC 1485 4.976547 13.293832 66
GGGCG 12155 4.919444 7.8581014 2
GCGGG 12115 4.903255 6.989032 28
TGCCC 65 4.8747334 189.17462 47
CCAGA 2650 4.8527384 307.06833 33
ACCAG 2630 4.816114 309.6794 32
GCACG 1135 4.622371 55.678146 30
CGACC 50 4.5569572 65.73411 66
ACCCG 50 4.5569572 65.67949 51
GACGG 24730 4.500463 6.226825 42
CGAAC 2450 4.4864936 11.851916 20
TCGTA 174555 4.348852 119.82868 43
GGCCG 475 4.3022 153.39885 47
CGTAT 166245 4.1418176 119.4943 44
TCGGG 27650 4.140577 10.8762665 3
GCCCT 55 4.124775 162.1405 48
CTCGC 55 4.1247745 80.95156 31
GCCTC 55 4.1247745 54.04539 52
TCGCC 55 4.1247745 107.93717 32
GACCA 2230 4.083625 247.79218 35
CACTG 2705 4.076063 200.06285 31
GGCCC 20 4.0538054 73.099205 71
CTCAC 115 3.8779964 60.709175 57
GTCCC 50 3.749795 161.88383 28
TCCAC 110 3.7093878 84.92583 30
CCCGA 40 3.6455657 98.51839 52
CACGG 870 3.5431392 114.21585 14
CCATC 105 3.5407794 36.42491 58
CTGAC 2325 3.5034552 204.41986 33
GGACG 19165 3.4877224 4.842031 4
CCTCT 125 3.4685838 79.98751 50
TCACA 5110 3.4623146 187.70184 30
GTCGG 23120 3.462211 5.653505 2
GGTCG 22910 3.430764 5.4950366 1
CGAGG 18755 3.4131088 4.5184584 33
CCGCT 45 3.374816 81.01698 56
CACCT 100 3.3721707 36.482018 69
TGACC 2220 3.3452346 204.42126 34
TCTCC 120 3.3298404 69.94344 40
TGAAC 108290 3.2786725 143.67886 20
AGAGA 1992375 3.2757614 3.6278718 43
CGGGA 17965 3.2693415 10.403824 4
GGCAC 800 3.2580593 71.794525 29
GCGAG 17765 3.2329452 4.131201 48
CCACT 95 3.2035623 24.266438 31
CGGAG 17525 3.189269 7.8519435 3
ACGGA 38530 3.1528556 4.1880984 60
TTCGG 56650 3.1388478 3.8126996 44
GGCGA 17080 3.1082857 4.9101977 1
TCGGT 55910 3.097846 3.7254157 3
GAACT 100275 3.0360045 143.46002 21
CTGAA 100265 3.0357015 143.18665 19
CGGTA 44930 3.0253398 3.7304661 1
TTTCG 147280 3.0193868 3.5019195 21
AGTCA 98745 2.9896812 142.05898 28
CCTTC 105 2.9136105 39.993862 52
CCCAT 85 2.8663452 84.94126 34
GAGCG 15645 2.847139 5.0383296 9
AGATC 92990 2.8154383 19.984049 72
ACACT 4080 2.7644312 154.56403 32
ACCGC 30 2.7341743 65.58559 32
TATGC 108550 2.704408 118.35139 46
CCCTA 80 2.6977365 36.481762 68
CTCCT 95 2.6361237 39.967857 41
CGTCA 1745 2.6294756 100.85438 33
CCCTG 35 2.6248567 54.08945 68
CTTGA 100500 2.5038507 116.15349 60
TCCCT 90 2.4973803 40.027462 67
TCTGA 99985 2.49102 117.43024 18
CAGGC 605 2.4639072 71.790825 27
CACTT 4270 2.3807082 126.59477 33
CCTAC 70 2.3605196 36.42491 58
ACCTC 70 2.3605196 48.51354 22
AGGCC 560 2.2806413 68.96325 46
CTCGG 680 2.2788227 84.490845 42
GACCC 25 2.2784786 65.73281 64
CTGCC 30 2.249877 81.016975 56
CCCTT 80 2.2198937 30.019848 68
CATCC 65 2.191911 24.321074 63
ACCAA 2640 2.1737866 112.03758 36
CCAGG 505 2.0566497 73.24747 26
CACAG 1110 2.0326564 90.934395 33
TTCTG 98975 2.029086 97.49885 55
TGCTT 98945 2.0284712 97.27297 58
GTCTT 97375 1.9962846 96.237404 52
TTCCC 70 1.942407 30.020002 64
ACGCT 1250 1.8835782 78.07458 32
CCTCG 25 1.8748977 81.03429 42
CCTGC 25 1.8748975 54.005154 58
CTACC 55 1.8546939 60.74132 43
GCGCA 450 1.8326583 51.250706 11
ACGAT 59180 1.7917801 6.863155 32
CAATC 2590 1.7548718 90.24328 38
GAAAA 2366730 1.7496933 5.1154766 63
CCGTG 520 1.7426292 83.31836 50
GGAAG 476190 1.7412031 18.905617 5
CGGCA 425 1.730844 54.179317 4
ACACA 2100 1.7291485 40.88515 32
TCACT 3095 1.7255952 84.44862 30
ATCCC 50 1.6860853 24.321302 64
CCTCA 50 1.6860852 36.448635 59
GCTTC 1350 1.6739407 58.97546 52
CATCT 2935 1.6363884 101.15417 39
CGCTA 1065 1.6048084 75.36966 33
TCATC 2805 1.5639079 94.52936 38
TCCTC 55 1.5261769 59.99079 52
TACCC 45 1.5174768 24.28367 57
CACAT 2125 1.439808 49.454346 12
TCCGA 945 1.423985 32.507805 2
CCGCA 15 1.3670871 32.866463 69
ATATC 120265 1.3472669 44.826042 38
GAAGA 814145 1.3385757 9.053002 6
GCTCG 385 1.290216 49.475956 41
TCCGG 380 1.2734598 32.54674 25
GACAC 670 1.2269187 19.094215 11
CAATA 86925 1.183386 53.567398 36
GCCGA 290 1.1810465 21.982061 33
GCCAG 290 1.1810464 24.903479 25
ACCCT 35 1.1802598 24.266832 32
AAGAG 710540 1.1682335 8.834865 7
ACTTC 2090 1.1652646 48.527775 23
CCGAT 760 1.1452155 14.671868 69
CATAC 1655 1.1213564 46.28732 12
AACAC 1305 1.0745422 10.393475 71
CTTCA 1925 1.0732701 47.72873 24
GTATG 927840 1.0329505 6.194758 45
CCGAA 525 0.9613915 38.84685 3
CCGGT 280 0.9383388 31.342142 26
TTCCA 1655 0.9227335 39.30601 24
ATTCC 1645 0.91715807 39.303486 23
CTGCG 270 0.90482664 33.78912 56
ACTCT 1555 0.8669792 35.89451 23
AGCTC 570 0.8589117 21.704033 40
GAGCC 205 0.8348776 21.964586 9
CTCTA 1490 0.8307389 35.696278 24
CATGC 550 0.82877433 35.24142 31
TACAC 1195 0.8096802 27.772392 12
TATCT 87205 0.80387485 36.611336 39
TTCCG 630 0.78117234 12.073298 67
CCGGA 190 0.7737891 8.785835 4
CCGGG 85 0.76986736 6.5158205 25
TGCCA 510 0.76849985 35.78703 33
ATCCG 480 0.723294 34.152214 1
CTCTC 25 0.69371676 10.0068655 66
GGCCA 170 0.6923376 16.118893 32
GTCCG 205 0.686998 12.049517 17
GGAGC 3425 0.623295 5.300061 7
GTGCC 185 0.61997384 22.940668 46
AAAAC 37375 0.61834425 5.1193657 71
GACTC 395 0.59521073 18.966154 21
ACCGG 135 0.5497975 5.8755918 72
CAGAC 295 0.5402105 6.584212 12
GCTGC 160 0.5361936 19.30807 56
CAACG 290 0.5310544 5.925791 12
AAACC 630 0.5187445 5.9391284 71
AAAGC 14090 0.51842755 8.134246 71
CTTCG 410 0.50838196 12.956597 53
CCAAG 275 0.50358605 10.544348 35
CCGTT 390 0.48358282 12.956696 50
CTTGC 385 0.47738305 15.642199 60
AGCCA 260 0.4761177 9.876318 10
ACTCG 290 0.43699014 7.596411 40
GAATC 14350 0.43447188 7.2149816 38
GCATC 285 0.42945585 17.900084 38
ACGCA 230 0.42118105 5.271139 32
GCTCT 330 0.4091855 10.731507 63
CGCTG 120 0.40214518 10.8445 16
ACCGT 250 0.37671563 5.4179683 15
GAACC 205 0.3754005 6.5853453 21
AGTCC 245 0.36918133 7.047445 28
GCAAC 200 0.36624438 12.510003 11
CCTGA 235 0.35411265 8.127049 18
CTCGA 225 0.33904403 5.427297 42
CGACT 195 0.2938382 5.972569 51
TGCTC 235 0.29138964 10.283715 62
CCGTA 190 0.28630385 9.23023 50
CTCAG 185 0.27876958 8.67198 24
TCCTG 220 0.27279034 5.350022 17
CTGTC 215 0.26659054 8.48887 50
GTCCT 190 0.23559164 5.349989 16
GTCTC 170 0.21079251 5.3578963 40
CAGAT 6290 0.19044098 5.185944 34
GATCA 6210 0.18801883 5.2095065 36
AAGCA 4320 0.15895009 7.7634645 72
GCCAT 90 0.13561763 7.04901 34
GATCT 4895 0.12195373 5.4528165 39
ACTTG 4690 0.11684637 5.7914324 34
Produced by FastQC (version 0.10.1)