FastQCFastQC Report
Mon 3 Feb 2014
filtered_Bs_CgLarve_T3D3_GCCAAT_L007_R2.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename filtered_Bs_CgLarve_T3D3_GCCAAT_L007_R2.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5837666
Filtered Sequences 0
Sequence length 76
%GC 17

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 181220 6554742.0 1.03181488E8 40
CGGGG 58940 119156.66 3296496.5 39
GGGCG 56660 114547.25 2546240.0 42
GGGGC 54545 110271.45 2445011.5 10
GGCGG 35270 71303.95 2502683.2 12
GGGGA 82675 60591.758 1407459.1 20
AGGGG 81170 59488.754 990170.6 27
GAGGG 74415 54538.08 1287301.0 27
GCGGG 24250 49025.26 2461178.0 13
GGTGG 50745 45431.11 2145550.2 40
GGGAG 54540 39971.87 873072.25 29
GTGGG 23780 21289.816 456801.7 41
GGGGT 19840 17762.404 507449.94 41
GGAGG 24060 17633.355 628781.4 17
TGGGG 16785 15027.316 390829.75 19
GGGTG 12925 11571.525 297309.53 16
GGCGC 30345 3428.8933 108502.72 44
TCGGG 57955 2900.078 63925.562 38
GAGCG 51675 2116.7935 91652.19 9
GCGGC 17760 2006.8263 46997.41 44
CGGTG 40010 2002.107 115823.25 39
GGCCG 17680 1997.7865 47129.58 12
GGGCC 16655 1881.9642 48921.934 11
GGTCG 37570 1880.009 101293.375 43
GCCGG 16060 1814.7314 63856.918 13
CGGCG 15405 1740.7184 38054.92 11
CCGGG 15175 1714.7291 65280.38 14
GGGAA 108340 1608.8593 73899.94 21
GGAAG 108110 1605.4438 50276.78 5
CGGGT 21975 1099.6327 54233.934 15
AGAGG 57760 857.7415 26241.48 26
AGGGA 57675 856.4792 44291.855 20
TAGGG 46965 851.97 45240.977 19
GTAGG 45415 823.8522 44100.656 18
AAGGG 52910 785.71857 11343.471 26
GGGTA 42670 774.0564 25915.375 29
CGGGA 18880 773.39264 33100.637 3
GTCGG 14705 735.84064 36299.15 13
GGTAG 40290 730.8819 24537.197 30
GGGTC 14220 711.5711 23009.83 42
GTGGT 27630 612.2821 37377.8 41
TGGGC 12135 607.23737 23659.01 42
GGAGA 40635 603.4336 17877.838 30
AGGGT 28040 508.66043 23190.469 28
GGCGT 9955 498.14987 9408.22 12
GCGCG 3930 444.07812 5026.371 45
AGCGG 10395 425.8165 13859.887 9
CGCCG 64100 404.84006 19602.91 46
CGTGG 7220 361.29 7606.0884 15
GAGTG 19805 359.27316 19291.873 27
AGGGC 8465 346.75677 13587.815 10
GCGTG 6515 326.0117 9047.752 13
GAGAG 19345 287.2751 6610.959 6
CGCGG 2480 280.2325 7436.9385 46
GTGGC 5490 274.7205 11161.273 41
GAAGG 15490 230.02798 6958.3413 6
GCGCC 31920 201.59898 5860.906 45
GCGGA 4445 182.08316 6992.131 47
CTGGG 3465 173.38918 5020.143 38
CTCGG 60405 168.94693 8923.744 37
TTGGG 6505 144.15112 3286.4854 37
GCCGT 49035 137.14615 7902.5044 47
GTGGA 7340 133.15149 2397.9153 16
ATCGG 127270 129.04305 3772.4265 2
ACGGG 3115 127.60159 4974.9893 2
CGTCG 44770 125.21734 5614.158 12
TGGGA 6765 122.72068 5084.387 3
GGTGC 2370 118.59519 3877.1716 43
TGGAG 6525 118.36695 2482.8552 16
GCGTC 41905 117.204216 5783.588 11
GATCG 113660 115.24345 3723.6643 1
GCGGT 2190 109.58797 2551.5693 47
TCGGA 105285 106.75178 3813.451 3
TGGCG 2120 106.08515 2903.278 42
GTCGC 34005 95.10868 5638.371 44
GACGG 2305 94.421074 2300.1514 9
AGGCG 2245 91.96326 1784.0918 9
CGGAA 104760 86.95277 3346.2864 4
GAGGA 5625 83.531784 1450.0215 28
CGGGC 695 78.5329 1017.91425 41
TGTGG 3315 73.46056 1468.6122 17
GGCAG 1780 72.91519 619.67725 12
GCCGC 11350 71.683846 2046.6461 44
GGACG 1745 71.48146 2598.1758 43
TGGTG 3165 70.13656 1472.5675 39
GTGCG 1390 69.55583 811.50116 43
ATGGG 3830 69.478226 3039.9548 2
GTGTG 3115 69.02856 1308.978 16
AGGAG 4565 67.79069 1866.3796 29
CGGAG 1640 67.18029 1612.5427 39
GGGCA 1615 66.1562 1490.2246 11
GTTGG 2775 61.494137 990.23334 1
GGTGT 2770 61.38333 1205.7002 40
GATGG 3150 57.142666 2843.7036 1
AGTGG 3015 54.693695 1797.4574 28
GCCCG 8225 51.947105 2797.8057 46
GGGAC 1255 51.4093 989.0467 42
AGAGC 60520 50.232735 2003.4819 8
GAGGC 1195 48.95149 973.71967 10
TCGGT 39110 48.441456 2787.3276 38
GCGAG 1160 47.51777 339.34732 17
CAGGG 1130 46.28886 1018.057 19
CCGCG 7145 45.126087 303.7804 45
GGGTT 1990 44.098495 687.0326 49
GAGGT 2420 43.90008 901.5272 31
GGGCT 865 43.28474 1118.0325 42
CGAGG 1055 43.216587 973.7181 18
TGGGT 1910 42.32569 814.47034 41
AAGAG 138090 41.550987 880.4914 25
AGCGT 40940 41.510357 2030.7365 10
GTCGT 32570 40.34104 1854.5032 13
GGTTG 1805 39.99889 510.8207 16
CGTGT 31585 39.121025 2013.3759 15
GGCTG 780 39.03133 1136.1016 43
GCAGG 910 37.276867 752.4185 18
TCGTG 29755 36.854397 1861.1663 14
GGAGC 870 35.63832 383.8214 43
CGCGC 5635 35.589294 155.30676 46
GGAAA 109520 32.954334 1306.744 22
GGTGA 1790 32.471546 509.5584 30
CGCGA 14180 32.46631 62.37338 72
GGGAT 1785 32.380844 699.01025 31
TGGTC 25820 31.980526 2027.758 42
GGCGA 770 31.541965 251.85826 44
GGCCC 4875 30.789318 909.05707 45
TCGCG 10850 30.346395 84.61905 14
GAAGA 98515 29.642954 887.2123 6
GTGAG 1625 29.47836 235.21786 16
AGGTG 1610 29.206251 471.70355 39
TGCGG 575 28.773096 253.00757 39
CGCGT 9100 25.451818 154.77977 48
CGGCC 3885 24.536718 1167.1562 45
CGACG 10495 24.029188 95.66054 12
ACGCG 9890 22.643991 53.345512 71
GCTGG 440 22.017673 324.551 40
GTGTA 48955 21.981516 710.9451 16
TGTAG 48845 21.932125 732.94104 17
GGAGT 1200 21.768635 274.3401 33
CCCGG 3090 19.515692 572.0294 37
GTCCG 6850 19.158785 72.56965 1
GAAAG 63175 19.009222 908.5904 23
TCCGG 6760 18.907064 936.5794 37
TGAGG 1020 18.503342 346.29358 17
CCGGA 8060 18.454052 699.6533 47
GCCGA 7645 17.503874 751.81683 47
GGATG 905 16.41718 222.15057 17
GTAGA 44285 16.277832 885.02606 31
AAAGG 44650 13.43509 235.71613 25
CCGTG 4480 12.530125 459.36057 14
AGCGC 5230 11.974526 637.42365 10
CCGGT 4060 11.355427 333.8387 48
TTCGG 9135 11.314567 408.94946 37
CCCCC 483205 9.533978 11.690152 14
TGGCC 3390 9.481501 410.21906 42
GACCG 3965 9.078202 221.94945 1
GACGC 3925 8.986619 154.8487 44
AGTGT 19820 8.899472 476.9403 28
CGGAT 8645 8.765438 459.52063 48
GCGCA 3805 8.711869 156.49051 47
GAGAT 23540 8.652594 262.09265 31
AAGCG 10205 8.470341 298.7595 9
AGAGT 22895 8.415511 395.5214 26
GCTCG 2775 7.7614055 77.71776 35
TCGGC 2715 7.593591 84.97007 44
GCGAC 3215 7.3610144 75.87119 11
TCGCC 45695 7.1433964 312.0176 45
GGATC 6895 6.9910574 319.46866 49
GCGTA 6860 6.95557 100.485756 48
GGTCC 2395 6.698582 249.96945 43
GCGAA 8025 6.6609006 13.090902 72
GAACG 7995 6.6359997 82.554756 1
CCGGC 1025 6.4736514 214.69543 47
GCGCT 2270 6.34897 45.52346 48
CCCCG 17800 6.2835364 65.87032 46
CGCCC 17100 6.036431 139.9192 45
TCTCG 85920 5.948155 216.27449 36
CTCTC 1513835 5.857674 7.7116857 71
CCGCC 15835 5.589876 107.62033 45
ACCGG 2400 5.4950027 211.23254 2
CGGCA 2355 5.3919716 64.32558 11
AGAGA 17335 5.2160645 54.144184 72
GTGCC 1830 5.1183324 197.25195 44
GACGA 6010 4.9884124 15.471065 72
GCGAT 4855 4.9226375 12.835772 48
GCACG 2150 4.9226065 15.731359 46
CCCGC 13465 4.753248 40.595768 47
GCGTT 3780 4.6818895 30.336864 11
GTTCG 3725 4.6137667 93.73328 1
CGGTC 1640 4.5869207 172.41766 49
GTCGA 4390 4.4511595 18.62503 13
GTACG 4305 4.364976 19.73093 1
CGGCT 1530 4.279262 212.44281 48
GGCTC 1530 4.2792616 137.12749 49
CCGTA 75325 4.2688055 157.30205 48
GAGAA 13660 4.110265 125.69758 31
TCTGG 3305 4.093557 125.58994 37
GAGCA 4855 4.029741 126.97279 9
GACGT 3925 3.9796813 29.58093 13
GGCCT 1395 3.90168 165.2112 12
ACGGC 1680 3.846502 189.66035 10
AACGG 4595 3.8139358 100.202324 1
TGCCG 1325 3.7058964 136.54228 46
CCCGA 28660 3.6676807 39.707462 47
CCGTC 23065 3.6056995 14.305462 48
CGGTT 2715 3.3627858 83.498604 49
TCTCT 1897570 3.2515948 4.76823 72
TGCGC 1140 3.1884692 41.473488 44
CTCCG 19515 3.050736 36.78185 36
GCCTG 1005 2.8108873 133.98224 13
CAGAG 3360 2.788863 35.70316 70
AGAAG 9245 2.7818007 24.159893 71
AGATC 134290 2.7589397 63.656006 33
CCGAT 47895 2.7142973 28.389801 48
AGGAA 9020 2.7140987 58.555202 21
CCTCG 17175 2.6849291 9.195462 36
CTCGC 17155 2.6818025 6.5001445 72
GTGTC 2095 2.5948567 33.026722 41
TCCCG 16410 2.5653384 13.652158 36
ACGGA 3090 2.564758 91.79845 3
CCTGG 915 2.5591664 80.58958 14
CCGCT 16255 2.5411077 28.158577 48
CCCGT 16230 2.5371993 34.259274 47
CGTAT 98405 2.4696555 71.75553 49
CGCTC 15795 2.4691968 15.159908 49
TCCGT 35550 2.4610908 8.468509 37
GCCCC 6830 2.4110425 26.427967 44
CCGAA 51735 2.400109 9.262557 48
TGTCG 1930 2.3904886 33.92351 43
TCCGC 15095 2.3597674 5.031939 44
AGACG 2835 2.3531032 47.19698 8
CTGTG 1860 2.303787 58.553547 38
GGTTC 1850 2.291401 77.68632 50
CGCAG 1000 2.2895844 30.634752 46
CGTGC 815 2.279476 34.252045 15
CGAGA 2740 2.2742512 26.776842 72
CGCTG 790 2.2095532 25.285608 46
CTGCG 780 2.1815841 18.206755 45
TTCCG 31220 2.161329 11.117264 36
AGCCG 935 2.1407614 49.677975 46
CACGG 930 2.1293135 33.047287 37
CCGCA 16555 2.1185784 18.65373 48
CGCCT 13510 2.1119878 10.401833 46
AAGGA 6890 2.0731864 50.846516 20
CGTGA 2015 2.043072 81.4411 15
GCAGA 2450 2.033546 22.909077 72
CTTGG 1635 2.025103 61.681988 36
ACGCC 15775 2.0187602 9.904025 45
ACGAG 2425 2.0127954 21.42142 71
CAGCG 845 1.934699 20.517504 71
ATCCG 34020 1.9279758 5.542254 36
AAGTG 5210 1.9150387 68.748375 27
TTTCG 62370 1.9121224 8.850744 36
CGGTA 1860 1.8859125 36.306896 48
GGTCT 1510 1.8702786 39.2466 33
GCTGC 660 1.8459558 56.646713 44
GGCAC 800 1.8316677 24.753454 43
CTTCG 26345 1.8238378 6.470497 36
CCGTT 26275 1.8189917 17.72828 48
CGATC 31875 1.8064146 13.52488 49
CGTCT 25985 1.7989153 6.563749 49
GAGCC 780 1.7858758 28.020082 9
ACTCG 31085 1.7616441 12.802471 35
AGCGA 2110 1.7513396 27.801157 10
GAGAC 2110 1.7513396 26.181734 71
CGTCA 30435 1.7248071 7.450378 12
GATCT 67465 1.693159 76.6212 34
GGTAA 4595 1.6889833 34.019066 30
TAGAG 4580 1.6834698 35.4813 19
CACGC 13010 1.6649171 5.7798266 72
TTTGG 3030 1.6619729 44.932137 36
GGTCA 1625 1.6476387 64.3261 50
CGTTT 53355 1.635743 7.416017 49
ATTGG 3640 1.6344135 55.177254 1
AAGGC 1960 1.6268367 42.41754 8
CGCTT 23420 1.6213429 7.5869946 49
ACACG 34040 1.5791963 6.136218 71
CACGA 33605 1.5590154 5.5043173 72
TGGAA 4190 1.5401175 35.746395 4
GCAGC 670 1.5340216 29.810442 44
GGATT 3360 1.5086893 59.56849 49
GAGCT 1470 1.4904794 36.508892 33
CGGAC 650 1.48823 12.376332 42
CGTTG 1200 1.4863142 24.982483 12
CCGAG 645 1.476782 29.687296 14
ACGTG 1455 1.4752705 31.406431 14
CATCG 25505 1.4454153 10.512178 12
CGTAG 1415 1.434713 20.085833 17
AGGTC 1410 1.4296433 29.210854 32
CGATT 56570 1.4197289 7.627022 49
CGAGC 620 1.4195423 11.489801 71
GTAAG 3820 1.4041169 48.98159 18
ATAGG 3815 1.4022789 51.23209 18
GTGAA 3800 1.3967654 36.136642 29
GACAG 1655 1.373681 15.173543 72
TCGAG 1345 1.3637378 12.781685 14
AGCAG 1640 1.3612306 13.161481 2
CAGTG 1325 1.3434595 12.816322 39
CGCAT 23375 1.324704 10.433508 48
GGCAT 1295 1.3130413 47.47557 12
AGTCG 1285 1.3029019 18.625029 12
GTATC 51275 1.286841 65.78684 50
TAAGG 3425 1.2589266 48.455807 19
TGTGT 2285 1.2533361 12.249072 15
TAGGA 3365 1.2368724 45.27838 19
GCTGT 970 1.2014372 12.989633 47
TGAAG 3235 1.1890885 32.171013 17
TGCGT 950 1.1766653 6.6596227 72
GGACC 510 1.167688 26.40368 43
GAGTA 3155 1.1596828 25.679518 29
AGTGA 3080 1.132115 32.71243 28
GTGCA 1110 1.125464 10.903323 72
AGGAC 1345 1.1163752 28.097095 32
TGTGC 900 1.1147357 12.49774 42
TTGGA 2470 1.1090664 29.440216 3
GTCTG 895 1.1085427 11.5990095 13
GAGTC 1070 1.0849066 17.530014 11
ATGCG 1070 1.0849066 10.53985 71
CTGGT 870 1.0775777 26.818419 38
GGACT 1055 1.0696977 40.889957 33
TACGG 1050 1.064628 17.92767 37
GGCCA 460 1.053209 31.337076 12
AGGCC 460 1.0532088 16.501776 42
GTGAC 1025 1.0392798 9.449522 71
GGAAC 1235 1.0250732 16.140884 32
GTTGT 1860 1.020221 11.458494 13
AGATG 2765 1.0163306 23.715996 72
ATCTC 718795 1.0082841 5.225597 35
TGGAC 940 0.9530956 44.21178 42
GCATG 925 0.9378866 31.406912 13
ACGGT 920 0.932817 13.172362 38
TGCGA 915 0.9277473 9.81299 72
AGTAG 2520 0.9262759 18.399416 30
GTGTT 1680 0.9214899 13.631643 16
CTGGA 905 0.917608 18.625544 15
GGTTT 1665 0.9132623 13.446164 49
GTTGC 735 0.9103675 18.814297 44
TGTTG 1655 0.9077773 13.829229 17
TTGGT 1640 0.89954966 23.950754 38
AGTGC 880 0.8922597 14.174283 71
CGAAG 1070 0.88812 6.545433 71
TAGCG 860 0.871981 6.542122 70
TTGTG 1570 0.86115426 11.458315 14
GCCCT 5480 0.85667604 13.058823 46
ACTGG 835 0.84663296 9.865898 2
CGAGT 835 0.8466328 12.417028 15
GGCTT 680 0.8422447 33.488743 49
TCAGG 805 0.8162148 10.976125 37
GAAGC 975 0.80926824 7.4379926 71
GCCAG 350 0.8013545 17.317856 13
GAAAA 130280 0.79430395 9.154535 23
TGACG 765 0.7756577 5.8463726 43
CAGGA 930 0.77191746 7.437993 71
GGAAT 2085 0.76638305 9.795289 32
GATAG 2075 0.76270735 9.272204 1
CCAGG 330 0.75556284 15.668295 14
TGAGA 2055 0.7553561 10.672198 72
AAGGT 2050 0.7535181 15.233263 27
GAAGT 2035 0.74800456 15.222553 31
TTAGG 1650 0.74087423 22.64018 18
GCAGT 730 0.74017 9.533481 47
CTGAG 730 0.74016994 5.858889 39
GGACA 885 0.73456657 5.0850434 43
ACAGG 880 0.7304165 7.18817 37
GGTAC 715 0.72496104 15.346727 43
ATGGA 1965 0.72227466 11.92193 2
GTAGC 710 0.71989137 15.401648 44
TGCAG 710 0.7198913 9.495121 17
ATGTG 1595 0.71617854 6.75986 71
CGATG 700 0.709752 6.5912504 39
GGCAA 850 0.705516 11.96852 22
AATGG 1910 0.7020584 15.762748 1
GATGT 1545 0.6937278 10.3473835 33
GATTG 1525 0.68474746 24.10971 1
GTCCC 4380 0.6847155 13.060426 44
GACTG 670 0.67933404 7.673138 1
CTGGC 240 0.67125666 8.062538 41
GTTTG 1220 0.66917723 13.638782 1
TAGAT 73455 0.6683005 21.421064 32
GGATA 1805 0.66346353 8.605643 15
GGTTA 1450 0.65107137 11.329861 50
GGTAT 1435 0.64433616 13.597157 49
CATGG 635 0.64384663 11.320923 14
GTAGT 1420 0.63760096 10.67221 31
GATGC 625 0.63370717 10.634471 44
AAAGA 103570 0.6314558 16.570478 24
GCAAG 740 0.6142138 6.8822117 23
ATGAG 1670 0.6138416 6.983024 71
GCTCC 3885 0.6073333 5.8041577 50
GAGTT 1345 0.60392475 11.964162 33
AGGTA 1635 0.60097665 13.773653 28
TGGCT 475 0.58833265 16.51487 42
TTGGC 475 0.58833265 6.2488694 42
CTAGG 580 0.5880802 19.025284 37
TGCTG 470 0.58213973 5.7719297 59
AAAAG 93585 0.5705782 5.1344304 25
AGGAT 1545 0.56789535 13.766353 32
GATCA 27585 0.5667238 9.005297 50
TGGCA 545 0.5525927 14.615464 42
GCATC 9460 0.5361155 9.410237 11
TATGG 1155 0.51861197 5.983476 18
AGCTG 510 0.51710504 5.0884433 59
AGGTT 1115 0.50065136 6.6296425 32
TGGTT 910 0.49914035 10.6738 42
CAGGT 485 0.49175677 9.513372 38
GTTAG 1090 0.48942608 8.409739 17
GCTTC 6885 0.47664163 5.564919 50
GAATG 1245 0.45762447 5.960704 1
TGGTA 995 0.44676965 8.737724 42
CAAGG 535 0.44406003 5.6797967 18
TGGAT 915 0.4108485 7.4381356 32
GTTGA 825 0.37043718 5.794415 60
TAGGT 820 0.36819208 8.587939 38
TCAGA 17325 0.35593584 6.635173 72
GATTC 14155 0.35524595 5.2832117 50
GAACT 17255 0.35449776 5.6443386 33
ATCAG 13390 0.27509266 5.714629 71
AAAGC 14540 0.24453562 6.785043 8
TGCCC 1515 0.23683654 8.989092 45
Produced by FastQC (version 0.10.1)