FastQCFastQC Report
Mon 3 Feb 2014
Bs_CgLarve_T3D3_GCCAAT_L007_R1_001.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename Bs_CgLarve_T3D3_GCCAAT_L007_R1_001.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6143478
Filtered Sequences 0
Sequence length 76
%GC 17

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC 43030 0.7004175810509943 TruSeq Adapter, Index 6 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC 23355 0.38015925181143323 TruSeq Adapter, Index 6 (100% over 50bp)

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CCCCC 26750 939890.8 3379882.5 1
CCCCA 8260 7185.0825 39759.582 32
ACCCC 7295 6345.663 46646.535 31
CCGCC 3185 6098.9834 38593.793 31
CACCC 6985 6076.0054 41634.008 30
CGCCC 3085 5907.492 65472.355 32
CCCGC 3050 5840.4707 32388.49 30
CCCAC 6450 5610.6274 14396.131 25
CCCCG 2885 5524.5107 18600.727 24
CCACC 6065 5275.7295 17217.07 30
GCCCC 2570 4921.3145 13093.219 30
ACGCC 92970 4407.4653 183178.69 32
CACGC 92565 4388.265 182801.23 31
CGCCA 92310 4376.177 183230.5 33
CCGTC 107145 4155.3516 185811.72 50
CACAC 122320 2634.191 103767.984 12
ACTCC 107015 1885.3165 82756.98 23
CTCCA 106130 1869.7252 82528.15 24
CTCCC 1775 1263.1062 22783.47 23
CCCCT 1725 1227.5258 4852.978 1
CCCTC 1635 1163.481 7951.0903 49
CCTCC 1335 949.9982 3580.5376 15
TCCCC 1315 935.76605 6130.0776 1
CCGGC 5270 549.9877 14444.316 49
CGCGC 4145 432.58044 1464.7106 30
CGCCG 2915 304.2152 1277.0493 32
GCCGC 2895 302.12796 1279.0327 48
GCGCC 2800 292.21356 1464.834 31
CCGCG 2720 283.86462 750.36444 16
CCCGG 2500 260.90497 1351.7025 25
CGGCC 2390 249.42516 637.4465 12
GCCCG 2295 239.51077 901.3441 29
TGCCG 110415 233.37714 10312.143 48
GGCCC 2220 231.68362 751.0956 28
GCCGT 108195 228.68486 10220.607 49
CACCA 8180 176.1583 4371.297 31
CGTCT 216455 170.01663 3775.6138 16
AGCAC 118585 139.17906 5745.1987 10
GCACA 116435 136.65567 5734.8633 11
ACACG 113860 133.63348 5699.124 13
CCCAA 5920 127.48864 1030.9314 33
CGCAC 2510 118.99255 1260.4274 11
ACACC 5460 117.58243 394.61392 12
CCACA 5325 114.67517 193.85603 36
ACGTC 114880 110.300735 4622.9604 15
CACGT 113110 108.60129 4633.739 14
GCCAA 92395 108.44077 4565.0015 34
ACCAC 4900 105.5227 239.90154 14
ATGCC 109855 105.476036 4679.4893 47
AACCC 4855 104.5536 658.49756 22
GTCAC 108775 104.43909 4587.1006 29
TCCAG 108265 103.94942 4568.073 25
CAGTC 107780 103.48375 4560.5166 27
CCAGT 107660 103.36853 4552.6587 26
CCAAC 4705 101.32332 248.07364 35
CAACC 4515 97.23163 232.34277 20
CCCAG 1995 94.57776 801.6415 25
GCCCA 1975 93.62961 1501.6096 33
ACCCA 4330 93.247604 255.77032 32
TCACG 96570 92.7206 3913.0647 30
CCACG 1950 92.44442 392.39075 30
GCACC 1920 91.022194 460.62433 29
GCCAC 1920 91.022194 460.62433 29
TCTGC 109330 85.87428 3822.0845 56
CTGCT 109095 85.6897 3823.6738 57
CTCGT 108455 85.187004 3779.2925 42
TCTCG 108425 85.163445 3779.5088 41
CCGAC 1770 83.91108 632.2585 50
CACCG 1750 82.962944 340.72003 14
TCACC 4585 80.77537 3525.0012 30
TCCCG 1995 77.3711 1185.968 24
ACCCG 1600 75.85184 323.6325 12
CCAGC 1585 75.14072 494.64398 26
CGCCT 1875 72.7172 391.42377 51
ACCGC 1530 72.53331 205.04573 49
CCCGT 1850 71.747635 1074.3922 25
CCGCA 1465 69.451836 170.21178 7
CGACC 1460 69.21479 221.5843 19
CCCGA 1415 67.08147 238.6277 18
CAGCC 1395 66.13331 443.53262 27
AGCCC 1385 65.65924 238.37093 9
GACCC 1365 64.71109 306.5992 12
CTCCG 1645 63.797222 627.8654 24
CGGCG 10425 59.294273 98.21239 23
CGCGG 10380 59.03833 108.24967 4
GCTCC 1510 58.561584 488.3062 23
CTCGC 1480 57.39811 181.64444 42
CCGCT 1480 57.39811 279.5843 50
GCGCG 9975 56.73481 95.99499 4
CGCTC 1440 55.84681 153.55374 34
CGTCC 1430 55.458984 334.52283 15
CTGCC 1395 54.101597 167.73479 46
TCCGC 1390 53.907684 237.54259 55
TCGCC 1335 51.774647 195.3929 30
TGCCC 1320 51.19291 657.08496 47
GCCCT 1250 48.478134 433.36917 48
AACTC 109380 47.70628 2081.5933 22
CCTGC 1165 45.18162 153.77136 50
GCCTC 1135 44.018143 153.77452 52
CCTCG 1125 43.630318 195.5697 41
GTCCC 1120 43.436405 432.6338 28
CCCTG 1105 42.854668 125.56445 23
CCAAT 94525 41.22725 1693.6326 35
ATCTC 107910 38.502525 1714.6667 40
TCGCG 17695 37.40079 59.38305 41
GCCGG 6380 36.28753 799.57965 48
GCGGC 6055 34.43903 94.30137 49
CGCGA 13085 33.807587 50.218357 33
CTTCT 110625 32.290188 1414.9152 54
CGTCG 14945 31.588293 75.204544 15
TCTTC 107930 31.503548 1402.1752 53
CGGGC 5270 29.974184 59.352757 27
CGACG 11600 29.970806 41.951317 72
GGCGC 5175 29.433851 63.457775 32
CGCGT 12990 27.456133 54.687027 14
ACGCG 10345 26.728275 77.05461 13
GGCCG 4625 26.305614 155.82533 47
CCCAT 1235 21.75738 507.29602 34
CCTTC 1455 20.96971 109.0941 53
CCTCT 1450 20.89765 155.84668 50
CACTC 1170 20.612253 95.10696 31
GGGCC 3585 20.390406 59.36304 31
CGGAC 7890 20.385315 47.317112 3
CTCAC 1150 20.259907 56.96847 12
CCGGG 3525 20.049143 51.15793 25
CTCCT 1330 19.168188 129.7798 41
CTTCC 1325 19.096127 103.98727 66
TCCAC 1070 18.850523 101.43888 30
CACCT 1035 18.233917 69.63256 13
CCACT 1020 17.969658 63.412003 34
CCCTT 1245 17.943153 51.87495 33
ACCTC 1005 17.705397 88.63173 14
CCATC 990 17.441137 114.09347 26
ATCCC 965 17.000706 158.44113 23
TCCCA 955 16.824532 145.77585 24
ACCCT 955 16.824532 50.61163 5
TTCCC 1145 16.501936 62.34454 47
CCTAC 900 15.855579 56.927956 7
CTCTC 1095 15.781328 57.085594 39
TCTCC 1090 15.709268 109.01342 40
CCCTA 865 15.238974 69.58543 6
TACCC 865 15.238974 63.3993 30
ACGGC 5890 15.217934 202.40372 14
CGGCT 6920 14.626362 308.5547 50
CCTCA 825 14.534282 38.0472 34
CTACC 810 14.270021 101.57895 43
TCCCT 930 13.403319 41.428528 13
CATCC 760 13.389156 63.393654 27
TCCTC 925 13.331259 88.31493 52
GCGTC 6260 13.231362 53.32756 49
GCACG 4665 12.052915 133.88748 29
CGGAA 188075 12.03011 322.7222 4
TCGGC 5505 11.635567 47.81657 3
ATCGG 220095 11.516976 267.8468 2
GGGGG 664380 11.223853 12.703478 1
AGCGC 4265 11.019438 70.52339 9
CACGG 4225 10.916091 194.02908 13
CGGTC 5090 10.758409 42.591644 27
TCGGA 204195 10.684973 264.04263 3
CGAGC 4065 10.5027 39.888927 7
GTCGC 4930 10.420226 40.312817 29
GGCAC 4010 10.360598 158.05644 28
GACGC 3900 10.076391 51.138493 30
TGCGC 4670 9.870681 20.572248 47
GCGAC 3595 9.288366 18.588997 22
GATCG 175520 9.184487 266.21585 1
AGAGC 139385 8.91568 318.96606 8
CAGGC 3385 8.7457905 158.95903 26
CGTGC 3985 8.42284 15.211302 27
CGGGG 26285 8.147777 12.156436 26
GAGCA 126640 8.100454 317.11926 9
CGATC 8295 7.9643507 178.67047 33
TTCGC 10015 7.866376 13.5611725 21
CCAGG 2975 7.6864786 164.53183 25
GCGCA 2925 7.557294 66.82417 10
CGGCA 2880 7.4410276 68.81111 32
ACGAC 6220 7.3001957 11.434062 72
GGCGG 23180 7.185294 10.148237 24
GGGCG 22920 7.104699 11.020137 5
AGGCC 2715 7.014718 65.18451 46
GCGGG 22590 7.002407 10.247054 13
CTCGG 3040 6.425454 124.095345 41
TACGC 6545 6.2841077 64.96407 31
CACGA 5325 6.249766 212.8904 31
AACGC 5265 6.1793456 23.218763 21
TCGTC 7825 6.14622 21.800692 52
GCTCG 2890 6.1084085 56.337765 41
CGTTC 7770 6.10302 9.598013 15
GCCGA 2335 6.0329165 35.3873 49
ATCGC 6265 6.015269 16.24936 39
GAGAG 1704970 5.9435997 6.613883 42
CTGCG 2805 5.9287496 58.62384 56
ACACA 10980 5.8539658 33.195583 32
CCGTG 2755 5.8230677 70.8533 50
GCTGC 2755 5.8230677 31.97664 56
CCAGA 4890 5.7392216 234.03574 33
GCCAG 2220 5.735792 44.616943 24
ACCAG 4790 5.621855 236.12518 32
CGCAG 2160 5.580771 12.106836 50
CGCTG 2635 5.5694313 11.418855 58
CAGCG 2150 5.554934 12.116837 70
GCGCT 2535 5.3580675 13.703579 40
GCAGC 2070 5.348238 12.1039715 43
CCGGA 2065 5.3353205 26.905807 3
CGTAC 5525 5.304766 8.991008 58
GGCCA 2025 5.2319727 34.408875 33
GACCG 2010 5.1932173 12.076147 17
TCGAC 5370 5.155945 8.9976845 49
TCCGG 2435 5.146704 36.501247 25
GACCA 4300 5.046759 190.96922 35
GAGCC 1940 5.0123587 22.270544 9
CCGAG 1935 4.9994407 12.069946 14
CCGGT 2365 4.9987497 39.54415 26
GTCTG 115715 4.95345 208.91547 17
ACCGG 1915 4.947767 15.765311 1
AACAC 9110 4.856979 20.68001 9
GTGCC 2280 4.8190904 47.231087 46
GCTTG 111290 4.7640276 207.63542 59
AGCCG 1840 4.75399 24.215952 47
GCCTG 2240 4.734545 14.438711 17
CTGGC 2240 4.734545 11.410584 32
CGAAC 4025 4.7240014 11.81549 19
CACTG 4825 4.6326694 154.1169 31
TGGCC 2165 4.576022 13.690699 28
ACCAA 8530 4.547753 92.72236 36
GGACC 1690 4.3664365 14.849817 10
GTCCG 2065 4.364659 13.677372 16
GGCTC 2030 4.2906814 8.37441 40
GGGGC 13760 4.265299 7.6900597 19
CACAA 7920 4.2225327 10.578354 68
GGCCT 1970 4.163863 10.625376 6
GACAC 3545 4.160642 50.180054 11
CACAG 3465 4.066749 68.85889 33
GACGG 28865 4.0644927 5.073149 42
TCGGG 34730 4.000639 10.837609 3
CCTGG 1885 3.984204 9.88906 31
CCAAA 7305 3.8946466 33.774765 34
CTGAC 4025 3.8645582 155.1703 33
TGACC 4015 3.8549569 155.51717 34
ACAAC 7155 3.8146744 9.809076 69
CAACA 7015 3.7400336 7.494787 50
TCGTA 188625 3.6679306 96.00339 43
CAAAC 6740 3.593418 8.811416 11
TCACA 8080 3.5241067 148.79588 30
CGTAT 180370 3.5074067 95.98203 44
GGTCC 1625 3.4346588 9.127132 28
GCGAG 23840 3.3569202 4.359273 35
GCTTC 4270 3.3539119 105.60595 52
GGACG 23790 3.3498797 5.10705 4
GGTCG 28920 3.3313699 4.796183 1
AAACC 6240 3.326844 9.588488 21
GTCGG 28775 3.314667 5.7562456 16
CGAGG 23170 3.2625773 4.5069513 20
CGGGA 23025 3.24216 10.921849 3
AGAGA 2038875 3.2286959 3.5632453 41
AACCA 6010 3.2042198 7.8872266 72
GGCGA 22400 3.1541536 4.3003736 12
CGGAG 22370 3.149929 8.140822 3
GAGCG 22050 3.1048696 6.5736566 8
ACACT 7080 3.0879548 124.008415 32
CGTCA 3105 2.9812307 61.169582 33
TGAAC 121880 2.8971066 116.3024 20
GGCGT 23880 2.7507997 5.3564215 48
ACGCT 2860 2.7459967 52.8703 32
CAGAC 2335 2.7405076 13.915477 11
CCGAA 2325 2.728771 60.68959 3
CTGAA 114050 2.7109861 116.25625 19
GAACT 113040 2.686978 115.8178 21
AGTCA 110865 2.6352782 114.67113 28
TGGCG 22710 2.616024 5.730486 47
CGCAA 2185 2.564458 8.448943 33
CACTT 7145 2.5493515 101.20035 33
ACGCA 2140 2.511643 8.448129 32
CGCTA 2540 2.4387524 50.45627 33
TCCGA 2515 2.414749 49.64636 2
TATGC 121530 2.3632267 94.92374 46
AGATC 98170 2.333516 16.072899 72
CGACA 1985 2.3297248 7.19198 54
GCGTG 19825 2.2836933 5.3934793 58
CAACG 1925 2.259305 6.326404 14
TCTGA 113470 2.2064948 94.58063 18
CAAGC 1875 2.2006218 7.602589 30
CTTGA 112960 2.1965775 92.80339 60
ACAGC 1865 2.1888852 5.5052896 72
AGCTC 2255 2.1651127 24.546906 39
GCAAC 1835 2.153675 5.4818544 11
CGGAT 41025 2.1467276 5.0159607 43
CCAAG 1820 2.1360703 19.43273 34
GAACC 1810 2.1243336 17.308533 21
CAGCA 1745 2.0480454 5.505289 72
ACTCG 2065 1.9826864 39.424854 40
AGCCA 1680 1.971757 11.82738 32
TTCCG 2485 1.9518667 18.121391 47
CGAAG 29710 1.9003831 5.053301 4
ATCCG 1970 1.8914733 55.829296 1
TGCAC 1970 1.8914733 5.8633194 10
GACTC 1965 1.8866725 31.081984 21
CATGC 1935 1.8578684 28.335392 31
TCACT 5200 1.8553714 63.559074 30
AACCG 1580 1.8543906 5.504761 71
AAGCC 1570 1.842654 11.827244 31
CCGTT 2325 1.8261932 12.457284 50
CACAT 4175 1.8209338 32.595184 12
TGCTT 114250 1.8174726 77.536606 58
TTCTG 114045 1.8142115 77.83378 55
CAATC 4135 1.8034877 67.53121 38
GAAAA 2478695 1.7830495 5.123206 63
GTCTT 111885 1.7798505 76.80292 52
GGAAG 508955 1.7742394 18.750265 5
CTTCG 2250 1.7672838 15.005561 53
TGCCA 1835 1.7618545 30.757587 33
CGACT 1810 1.737851 12.113099 51
CTGTC 2200 1.7280108 13.872884 50
CGCTT 2175 1.7083744 5.662485 51
GCACT 1775 1.7042462 5.519427 11
CATCT 4735 1.6894583 76.18801 39
CCGAT 1745 1.675442 9.332082 35
CTTGC 2125 1.6691014 13.305292 59
TGCTC 2115 1.6612468 6.8001933 62
CTCTG 2105 1.6533922 7.078141 54
GCTCT 2090 1.6416103 6.2338576 63
TCATC 4410 1.5734977 70.91939 38
GCCTT 1980 1.5552098 7.078147 52
TCCGT 1975 1.5512824 14.439572 48
ACTGC 1610 1.5458233 9.671136 22
ACCGT 1600 1.5362219 5.5370364 49
ACGAT 63360 1.5060769 5.637724 32
GTCTC 1870 1.4688092 6.224117 40
ACTTC 4085 1.4575368 36.70978 23
CTGCA 1515 1.4546101 9.325799 23
CTCGA 1485 1.4258059 8.302101 42
CATAC 3175 1.384782 33.69214 12
AGTCC 1440 1.3825997 10.710304 27
CTCAG 1425 1.3681977 9.671864 24
GAAGA 848620 1.3438469 8.963734 6
TTGCC 1705 1.3392082 6.7942414 46
GCATC 1360 1.3057886 11.754693 38
TACAC 2980 1.2997324 17.394548 12
CTTCA 3620 1.2916238 36.840668 24
CCGTA 1265 1.2145754 8.99817 50
GCCAT 1265 1.2145754 7.603061 34
TCCTG 1540 1.2096076 5.0832677 18
AAGAG 745850 1.1811037 8.78717 7
GGAGC 8380 1.1799914 5.3590055 7
ATATC 131855 1.1647216 35.29951 38
GTCCA 1205 1.1569672 10.016599 23
CCTGA 1200 1.1521665 9.320613 18
ACTCT 3180 1.1346308 31.447193 23
ATTCC 3155 1.125711 29.906925 23
TACCG 1160 1.113761 6.921885 48
CTCTA 3010 1.0739745 31.064257 24
TTCCA 2965 1.0579184 29.908976 24
CAATA 97955 1.0576996 42.53851 36
GTATG 966410 1.0241832 6.0945673 45
ATCAC 2325 1.014053 6.1210394 28
AATCC 2290 0.99878764 28.869614 22
GGCTG 7645 0.8806474 9.441899 16
ATCCA 1980 0.86358064 24.476574 23
TCCAA 1695 0.73927736 7.8455944 24
TATCT 97460 0.70427424 28.594204 39
CCATA 1545 0.67385453 13.188573 35
AAAGC 19560 0.5683437 6.593923 71
GAATC 17395 0.4134818 6.7446065 38
GGCTT 8050 0.344599 6.37267 51
AAGCA 9635 0.27995867 6.3429346 72
ACTTG 8880 0.17267711 5.6834188 34
Produced by FastQC (version 0.10.1)