FastQCFastQC Report
Mon 3 Feb 2014
Bs_CgLarve_T3D3_GCCAAT_L007_R2_001.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename Bs_CgLarve_T3D3_GCCAAT_L007_R2_001.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6143478
Filtered Sequences 0
Sequence length 76
%GC 17

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 215800 5441917.0 8.0897016E7 40
CGGGG 65695 98409.48 2606505.0 39
GGGCG 64385 96447.125 2224460.2 42
GGGGC 62585 93750.77 2062915.5 10
GGCGG 39525 59207.465 2084458.8 12
GGGGA 96355 52408.473 1239781.6 20
AGGGG 95090 51720.426 879559.0 27
GAGGG 84160 45775.484 964064.06 27
GCGGG 27300 40894.72 2038080.2 13
GGTGG 56035 37329.42 1619688.2 40
GGGAG 63295 34426.797 752367.5 29
GTGGG 28180 18772.965 418967.7 40
GGAGG 28875 15705.407 560960.75 17
GGGGT 23210 15462.048 419926.7 41
TGGGG 21170 14103.039 342434.38 19
GGGTG 15800 10525.65 247418.2 16
GGCGC 34250 3047.6797 95992.164 43
TCGGG 64640 2557.9812 53400.777 38
GCGGC 20110 1789.4552 41343.22 44
GAGCG 55205 1783.6506 72525.24 9
GGCCG 19775 1759.6455 37790.84 12
CGGTG 42755 1691.932 92639.164 39
GGGCC 18645 1659.0945 39428.246 11
GGTCG 40540 1604.2784 81685.23 43
GCCGG 17785 1582.5687 50612.82 13
CGGCG 17235 1533.6278 38017.8 11
CCGGG 16160 1437.9707 51894.715 14
GGGAA 121860 1429.602 63468.797 21
GGAAG 117375 1376.9862 40349.984 5
CGGGT 23365 924.6167 41997.32 15
AGAGG 64470 756.3306 20814.791 26
TAGGG 51130 734.6736 36303.28 19
AGGGA 62450 732.633 35617.562 20
CGGGA 22485 726.481 33286.34 3
AAGGG 61160 717.4993 10665.466 26
GTAGG 49135 706.00793 35200.11 18
GGGTC 16635 658.29236 19611.984 42
GGGTA 45425 652.69995 20615.623 29
GGTAG 42600 612.1082 19420.432 30
GTCGG 15240 603.0884 27355.25 13
GGAGA 47740 560.0624 16743.25 30
TGGGC 13850 548.0823 18128.605 42
GTGGT 29915 526.4683 30013.717 41
GGCGT 11980 474.08124 11189.987 12
GCGCG 5300 471.6117 4958.287 45
AGGGT 31040 446.00565 18502.66 28
CGTGG 9675 382.86615 9550.77 14
AGCGG 11750 379.6376 14214.249 9
CGCCG 68565 362.42267 16728.254 46
GCGTG 8155 322.7156 10420.369 13
GAGTG 21870 314.24432 15725.298 27
AGGGC 9315 300.96378 11126.583 10
CGCGG 3360 298.984 6632.153 46
GAGAG 24245 284.43054 6833.0073 6
GTGGC 6380 252.47401 9356.031 41
GAAGG 17010 199.55304 5642.0874 6
GCGCC 35405 187.14465 5602.253 44
CTGGG 4615 182.62814 5844.5054 38
GCGGA 5210 168.33293 6230.8286 47
TTGGG 8865 156.01343 3196.345 37
CTCGG 65335 153.58401 7661.219 37
ACGGG 4375 141.35443 5298.9585 2
GTGGA 9610 138.08357 3079.8423 15
GGTGC 3155 124.85196 4464.375 43
GCCGT 51900 122.00214 6736.161 47
TGGGA 8450 121.41584 4835.997 3
TGGAG 8440 121.27214 3167.7517 16
ATCGG 138085 117.86077 3221.1968 2
GCGGT 2880 113.969444 2119.0679 47
CGTCG 47485 111.62374 4732.583 12
GCGTC 45140 106.11131 4907.3516 11
TGGCG 2570 101.70191 2524.5972 42
GATCG 116450 99.39448 3170.8374 1
TCGGA 112855 96.326004 3267.003 3
GACGG 2915 94.182434 1977.4323 9
AGGCG 2765 89.336006 1698.2655 9
GAGGA 7490 87.86903 1522.0854 28
GTCGC 36100 84.86084 4831.467 44
CGGGC 925 82.3096 897.96326 41
TGTGG 4610 81.1305 1793.453 39
CGGAA 112750 78.57325 2876.7578 4
CGGAG 2410 77.8661 1529.5383 39
TGGTG 4395 77.34675 1291.0317 39
GTGTG 4360 76.73079 1033.3606 16
GGACG 2270 73.34275 2364.0015 43
GGGCA 2260 73.01966 1443.267 11
AGGAG 6055 71.03431 1930.762 29
GCCGC 13380 70.72435 1824.701 44
GTGCG 1695 67.075775 741.6853 43
ATGGG 4620 66.38357 2724.2827 2
GGCAG 2040 65.91155 547.33044 43
GTTGG 3705 65.20358 1103.104 17
GGTGT 3365 59.219986 1109.9784 40
GGGAC 1745 56.380222 815.17755 42
AGTGG 3820 54.88858 1501.7552 28
GATGG 3790 54.45752 2320.1475 1
GGGCT 1360 53.818905 1212.3771 42
GCCCG 9390 49.633907 2694.6506 46
TGGGT 2810 49.45265 983.1205 41
CAGGG 1510 48.787468 977.62463 19
CGAGG 1480 47.81819 709.92725 18
GAGGC 1440 46.525803 942.7335 10
GCGAG 1440 46.525803 302.61377 17
GGGTT 2610 45.932888 622.0016 49
AGAGC 65700 45.785034 1703.2039 8
TCGGT 41835 43.7348 2380.8862 38
GGCTG 1090 43.134274 984.15936 43
CCGCG 8095 42.788765 260.1082 45
GAGGT 2955 42.45962 714.1036 31
GGAGC 1290 41.679363 407.58646 43
GGTTG 2360 41.53318 551.5483 16
GGGAT 2720 39.082966 750.3262 31
AAGAG 149970 37.947678 752.5829 25
AGCGT 44045 37.59407 1722.1099 10
GTCGT 35900 37.530277 1620.423 13
TGCGG 945 37.39623 371.8151 39
CGTGT 35235 36.83508 1735.3724 15
GCAGG 1135 36.671383 721.56537 18
GGTGA 2460 35.347095 496.7657 30
TCGTG 33655 35.18333 1637.7375 14
GGCGA 1075 34.732803 315.66446 44
CGCGC 6395 33.802856 166.38368 46
GCTGG 805 31.856047 413.60638 40
GGAAA 125515 31.759699 1169.3939 22
GTGAG 2110 30.318035 279.5075 16
AGGTG 2085 29.95882 472.5173 39
GGCCC 5545 29.309904 923.76654 45
CGCGA 14960 28.712242 59.72019 47
TGGTC 27365 28.60769 1728.7556 42
TCGCG 11650 27.38584 84.20493 45
GAAGA 107950 27.315142 755.2088 6
GGAGT 1860 26.725851 284.55838 33
CGCGT 10240 24.071327 179.4974 48
CGGCC 4380 23.151917 1097.8094 45
CGACG 11375 21.831669 90.56718 12
GTGTA 53720 20.39135 612.8623 16
TGTAG 53610 20.349598 638.5734 17
TGAGG 1410 20.259922 403.73575 17
ACGCG 10495 20.142714 43.972904 71
GGATG 1265 18.176453 201.86784 17
CCGGA 9260 17.772417 681.9212 47
GAAAG 69420 17.565699 772.6641 23
TCCGG 7425 17.454065 902.26855 37
GTCCG 7075 16.631315 58.46145 43
GCCGA 8440 16.198618 695.11523 47
CCCGG 3025 15.989625 454.24722 37
GTAGA 48185 14.9333515 758.9581 31
AAAGG 53985 13.660101 242.4854 7
CCGTG 5330 12.529314 395.44357 14
AGCGC 6130 11.76511 580.05615 10
TTCGG 10405 10.877508 385.02588 37
CCGGT 4355 10.237367 302.8487 48
CCCCC 506735 9.451528 11.764903 16
TGGCC 4000 9.4028635 372.7998 42
GACGC 4505 8.6463 147.23189 44
CGGAT 10015 8.548181 441.39822 48
AAGCG 12170 8.481032 262.1777 9
AGTGT 22125 8.398337 415.05737 28
GACCG 4255 8.166483 186.08301 1
GAGAT 26110 8.091933 263.06827 31
GCGCA 4205 8.07052 152.07816 47
AGAGT 25890 8.023751 343.00085 26
GCGAC 3840 7.3699875 77.436745 11
TCGGC 3130 7.3577404 95.268364 44
GCTCG 3110 7.310726 68.75354 35
GGATC 8240 7.033151 308.13354 49
TCGCC 48760 6.8087587 284.91058 45
GCGTA 7890 6.734413 84.94367 48
GGTCC 2835 6.6642795 230.45673 43
GCGCT 2715 6.382193 43.376797 47
GAACG 9100 6.34161 84.89727 1
CCGGC 1175 6.2108455 248.62419 47
CGCCC 19205 6.0301948 145.64893 45
GTGCC 2560 6.0178323 253.48189 44
GCGAA 8565 5.9687796 11.293516 29
CTCTC 1610425 5.94068 7.8218064 71
CCCCG 18760 5.890468 60.210392 46
TCTCG 92050 5.716304 198.08536 36
AGAGA 21540 5.4503765 52.99242 72
GCACG 2760 5.2971787 22.221027 46
ACCGG 2710 5.201215 191.6298 2
CCGCC 16500 5.1808496 103.158516 45
CGGCA 2670 5.1244445 67.06575 11
CGGCT 2075 4.8777347 239.04631 48
GGCTC 1990 4.6779246 152.6001 49
CCCGC 14770 4.6376452 39.421547 47
GCGAT 5430 4.6347103 18.533165 48
AACGG 6590 4.592441 135.88588 1
GAGAA 17735 4.487578 128.57938 31
TCTGG 4290 4.484816 156.6527 37
GACGA 6430 4.48094 14.719302 72
GCGTT 4200 4.390729 28.62164 11
GTTCG 4190 4.380275 88.92385 1
GAGCA 6125 4.2683916 122.18235 9
CGGTC 1790 4.2077813 139.88342 49
GTCGA 4890 4.1737995 19.062687 13
TGCCG 1750 4.113753 130.97833 46
GTACG 4690 4.0030923 14.459043 1
CCGTA 78440 3.977081 142.9171 48
GGCCT 1660 3.9021878 144.79721 12
GACGT 4510 3.8494554 28.901493 13
ACGGC 1960 3.7617643 176.98972 10
CCCGA 30805 3.5120513 41.99278 47
CCGTC 24075 3.3617897 13.138725 48
CGGTT 3205 3.3505445 82.19133 49
TCTCT 2015700 3.306806 4.8593197 72
TGCGC 1375 3.2322342 39.12808 44
AGGAA 12435 3.1464918 66.46562 20
CTCCG 21325 2.977785 41.39529 36
CAGAG 4270 2.9756784 38.429245 70
CTGTG 2785 2.9114714 85.31478 38
ACGGA 4160 2.8990216 94.67165 3
AGAAG 10950 2.7707345 21.558939 71
GCCTG 1160 2.72683 120.24254 13
GTGTC 2595 2.7128432 30.141796 41
CCTGG 1145 2.6915693 86.37097 14
CTCGC 19195 2.6803558 6.348705 72
AGCCG 1360 2.6102037 64.57986 46
AGATC 141080 2.5972571 57.817017 33
CTGCG 1100 2.5857873 27.217756 45
CCGAT 50175 2.5439835 29.39443 48
CCTCG 18165 2.5365279 8.92444 36
CCGCT 18095 2.5267532 28.955729 48
CGTGC 1050 2.4682517 31.332478 15
TGTCG 2345 2.4514904 32.781357 43
CGCTG 1040 2.4447443 23.814241 46
CGCTC 17225 2.405268 16.618828 49
TCCCG 17215 2.403872 11.747993 36
CTTGG 2290 2.3939927 77.760315 36
AAGGA 9445 2.3899167 49.233795 20
GCCCC 7495 2.3533616 27.946712 46
CCCGT 16805 2.3466198 31.425474 47
TCCGT 37715 2.3421013 9.910972 37
CGAGA 3360 2.3415177 20.207859 72
GGTTC 2235 2.3364952 72.753525 50
CGTAT 103605 2.3361182 64.83628 49
AGACG 3290 2.2927358 46.154564 8
GCTGC 970 2.2801943 58.692116 44
TCCGC 16260 2.2705176 5.153888 44
CCGAA 54610 2.2606559 9.168311 48
CGTGA 2625 2.2405367 83.634315 15
GAGCC 1155 2.216754 30.402517 9
CAGCG 1150 2.2071576 28.85722 71
CGCAG 1105 2.1207907 24.998653 46
ATTGG 5585 2.119987 74.700165 1
ACGAG 3025 2.1080625 19.459084 71
GGCAC 1095 2.101598 27.670424 43
CGCCT 14980 2.0917802 11.165422 46
CACGG 1075 2.0632126 42.96646 37
GGTCT 1965 2.054234 36.136715 33
CGGTA 2375 2.0271523 27.799747 48
CCGCA 17745 2.023092 20.088882 47
TTCCG 32565 2.0222862 10.875187 36
AAGTG 6340 1.9648737 62.897335 27
GCAGA 2805 1.9547491 19.95838 72
AGCGA 2785 1.9408113 27.61362 10
ACGCC 16950 1.9324547 9.735401 45
GGTAA 6165 1.9106382 31.809143 30
TGGAA 6130 1.8997911 43.107533 3
TTTGG 4080 1.8968579 43.814575 36
TAGAG 6100 1.8904936 35.50046 19
GAGAC 2690 1.8746079 27.442299 71
ACGTG 2185 1.86498 29.516273 14
ATCCG 36190 1.834913 6.0414934 36
GGTCA 2140 1.8265709 56.938213 50
CACCG 16015 1.8258561 5.197431 46
CCGAG 945 1.8137078 33.18519 14
AAGGC 2595 1.8084043 44.649525 8
TTTCG 65100 1.7978759 8.316647 36
CCGTT 28250 1.7543244 16.720144 48
CGTCT 28085 1.7440782 6.0693607 49
CTTCG 27815 1.7273111 7.0408425 36
GCAGC 885 1.6985519 28.474094 44
CGATC 33490 1.6980171 14.31762 49
ACTCG 33350 1.6909188 13.529484 35
GACAG 2425 1.6899345 16.714853 71
CACGC 14645 1.6696637 5.8773184 72
CGTCA 32500 1.6478219 7.433362 12
TGTGT 3540 1.6458032 10.886314 15
GATCT 72900 1.6437722 70.15551 34
CGGAC 845 1.6217811 13.143527 42
ATAGG 5170 1.6022708 50.79364 18
CGCTT 25520 1.5847914 7.547507 49
CGTAG 1855 1.5833126 20.908152 17
GGCAT 1840 1.5705096 53.497807 12
TCGAG 1825 1.5577065 16.910366 14
CGTTT 56230 1.552912 7.121426 49
CAGTG 1795 1.5321003 13.444513 71
ACACG 36845 1.5252494 6.2834444 71
GAGCT 1780 1.5192972 35.343594 33
GTAAG 4855 1.504647 45.770096 18
CACGA 36275 1.5016534 5.320272 72
CGAGC 770 1.4778361 8.245062 72
GTGAA 4740 1.4690065 33.25984 29
CGTTG 1405 1.4688034 22.667116 39
GGATT 3840 1.4576095 56.812023 49
TTGGA 3815 1.4481201 36.79449 3
AGGTC 1695 1.4467466 25.514046 32
TGTGC 1375 1.4374412 12.433493 41
TAAGG 4630 1.4349157 46.775764 19
AGTCG 1670 1.4254081 18.800203 35
CATCG 27425 1.3905082 11.013064 12
GAGTA 4425 1.3713828 27.56772 29
TAGGA 4420 1.3698332 46.999043 19
GGAAC 1965 1.3693696 19.329535 10
GAGTC 1580 1.3485898 16.6039 11
GGCCA 700 1.3434873 42.1725 12
CGATT 59095 1.3324927 7.3350463 49
GCTGT 1245 1.3015375 11.347384 47
CGCAT 25660 1.3010187 12.532082 48
AGCAG 1860 1.2961972 10.047683 2
GTGCA 1505 1.2845744 9.839066 16
GTGTT 2760 1.2831686 12.56079 16
GTCTG 1220 1.2754022 12.057511 43
GCATG 1465 1.250433 38.125378 13
TGTTG 2675 1.2436507 13.230699 16
AGGCC 645 1.2379276 20.741985 11
AGGAC 1775 1.2369624 30.117428 32
TGCGT 1175 1.2283587 8.69949 46
TGAAG 3950 1.2241721 33.604378 17
GGACT 1430 1.220559 46.100338 33
AGTGA 3915 1.2133251 31.050797 28
GTATC 53415 1.2044183 59.42668 50
CTGGT 1140 1.1917692 24.914936 38
GTGAC 1365 1.1650791 8.250042 71
AGTAG 3755 1.1637383 21.429317 30
AGATG 3725 1.1544408 21.63501 72
GGACC 600 1.1515605 21.444578 43
ATGCG 1340 1.1437407 10.083385 71
TTGTG 2415 1.1227725 11.723039 14
GTTGC 1070 1.1185907 24.96674 44
CGAGT 1295 1.1053315 17.21883 15
TGGAC 1275 1.0882607 44.915726 42
GTTGT 2335 1.0855793 11.723098 13
TTGGT 2325 1.0809301 22.831898 38
TGCGA 1255 1.0711899 9.16687 72
CGAAG 1510 1.0522892 8.536374 4
TAGCG 1230 1.0498515 7.94656 70
TACGG 1215 1.0370485 14.485151 37
GGCTT 975 1.0192764 38.07947 49
ATCTC 757495 1.0146074 5.109243 35
GGTTT 2175 1.0111926 14.755008 49
ACGGT 1165 0.9943715 14.177808 38
TTGCG 950 0.99314106 6.7364254 71
CTGGA 1155 0.98583615 18.756226 15
AGTGC 1130 0.96449775 8.5556 71
TGACG 1115 0.95169467 6.768085 43
GAAGC 1350 0.9407884 8.731641 71
GGACA 1350 0.94078827 6.530581 43
TGCAG 1095 0.9346239 10.146603 17
ATGTG 2460 0.9337812 6.11494 71
GTGCT 890 0.9304164 6.0287905 42
CAGGA 1330 0.9268507 6.780198 3
AAGGT 2945 0.9127056 19.215527 27
GTAGC 1055 0.9004824 17.913534 44
GGAAT 2905 0.9003087 10.268609 32
CGATG 1045 0.89194703 6.1689386 39
GCCCT 6370 0.8894953 16.268171 46
GATGT 2315 0.87874126 9.977529 33
GGATA 2805 0.8693171 10.941183 15
ACAGG 1245 0.8676158 6.79398 37
TCAGG 1015 0.8663409 16.33432 37
GATAG 2765 0.8569205 10.16496 1
AATGG 2730 0.8460734 22.563976 1
ATGGA 2670 0.8274784 13.405234 2
GAAGT 2625 0.8135322 15.513997 31
GAAAA 148705 0.81158334 9.668215 23
GCAGT 950 0.81086093 8.9558735 47
TTAGG 2135 0.8104158 27.072464 18
TGAGA 2580 0.7995859 7.766414 72
GGTAC 935 0.7980578 13.228528 43
CCAGG 415 0.79649603 17.325186 37
ACTGG 920 0.7852548 9.84511 2
GTTTG 1655 0.7694362 12.902808 1
AGGTA 2455 0.76084626 15.637092 28
GCCAG 395 0.7581107 12.444508 13
GATGC 885 0.755381 9.574475 44
GTAGT 1990 0.75537586 11.3462305 31
GGCAA 1080 0.7526307 11.560522 22
CATGG 880 0.7511133 11.0686035 14
TGTGA 1965 0.7458862 6.3868246 72
GGTTA 1935 0.73449856 12.181741 50
GCAAG 1035 0.7212711 6.2831664 23
CTAGG 840 0.71697176 21.265434 37
GATTG 1865 0.70792764 26.26819 1
TTGGC 675 0.705653 9.042541 41
GAGTT 1835 0.69654006 8.6107435 33
GGTAT 1815 0.6889483 9.58275 49
ATGAG 2160 0.66942066 6.6568117 71
GTCCC 4705 0.65699774 12.379436 44
GCTCC 4700 0.65629953 6.2939553 50
TAGAT 77895 0.63774395 19.625853 32
AGGAT 2055 0.63687944 12.500918 32
GACTG 740 0.63161796 7.075701 1
AAAGA 114465 0.6247126 14.986521 24
TGGTT 1335 0.62066305 10.389368 42
AAAAG 109290 0.5964691 5.769043 24
TATGG 1570 0.5959498 8.887425 18
TGGCA 695 0.59320873 12.920962 42
GCATC 11605 0.5883992 10.301479 11
CTGGC 245 0.57592535 7.624905 41
GTTAG 1510 0.57317466 11.896336 17
CAAGG 805 0.56098855 7.028599 18
AGGTT 1470 0.5579912 5.331545 32
TGGTA 1460 0.5541953 6.9775505 42
TGGCT 530 0.5540682 15.448775 42
GATCA 29785 0.5483365 9.247196 50
GAATG 1700 0.52685887 6.9255767 1
CAGGT 615 0.5249257 9.862823 38
GTTGA 1365 0.5181347 5.029722 60
GCTTG 485 0.50702465 5.6622434 35
TGATG 1295 0.49156368 5.4695797 17
TGGAT 1290 0.48966572 5.4680624 31
GATCC 9650 0.48927635 5.320216 50
TAGGT 1235 0.46878853 9.457745 38
GCTTC 7370 0.45767692 5.0830774 50
AGGCT 460 0.3926274 6.4530253 9
GAACT 19325 0.35576972 6.2178817 33
GCTCA 7005 0.355169 5.685867 50
GATTC 14995 0.338112 5.39873 50
ACAGA 21930 0.32962754 5.0796523 72
TCAGA 17785 0.32741866 5.977698 72
CTCTG 4875 0.30273747 6.2111897 36
TGCCC 1945 0.2715963 11.317604 45
ATCAG 14455 0.26611397 5.166962 71
AAAGC 17115 0.25725383 6.508625 8
Produced by FastQC (version 0.10.1)