FastQCFastQC Report
Mon 24 Mar 2014
BiGoRNA_GTGTCTAC_2.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename BiGoRNA_GTGTCTAC_2.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 25542180
Filtered Sequences 0
Sequence length 50
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 564422 2.2097643975572954 Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGACTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGT 300638 1.1770256101867576 No Hit
GGACTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTAT 159005 0.6225192994489899 No Hit
GGGACTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTA 142359 0.5573486679680434 No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG 81870 0.32052863146372 Illumina Single End PCR Primer 1 (98% over 50bp)
CTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGG 67912 0.26588176890147985 No Hit
AATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGGAGCATTAG 59011 0.23103352963607646 No Hit
CTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGGAGC 56836 0.22251820322306085 No Hit
TAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGGAGCA 49995 0.19573505472124933 No Hit
GACTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATA 46077 0.1803957219000101 No Hit
GCAAAATTAAACCTCGCCTGTTTAACAAAAACATCACTAGAAGATAAAGA 39880 0.15613389303497197 No Hit
AAAGCATTGTTTTTAAGCGTTAAAAAAAAGTACCTTTAGTATAATGGCCT 39797 0.15580894034886608 No Hit
ACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGGAG 39572 0.15492804451303688 No Hit
TTTAAAAACTTTTTCTAAATGAAGTATAGGAGCATTAGAAACATTATTTT 38759 0.15174507422624067 No Hit
CTTTAATGTGGGGCGTAGTGTGTTAAAGATACTCACTAGACCAGGATGTT 37058 0.1450855017073719 No Hit
CTTGAATAAACTTTGCTAAGGATGTGATATGCAATTTCGAGTTTTAAGAT 36215 0.14178507864246515 No Hit
GCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGGAGCATT 34945 0.13681291103578472 No Hit
TTTCAAGAGATTTTGCTGTATTAGCACCTCCCGAAATAAAAAGATTTTTA 34384 0.13461654408511725 No Hit
ACTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAG 32028 0.12539258591083455 No Hit
TTATAAGTGAAAAATTCGGCTAAAGGTTGGATTTAAAATGCTTTCTAAGT 31068 0.12163409701129661 No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGTCGCCG 30815 0.1206435785825642 Illumina Single End PCR Primer 1 (98% over 50bp)
CGAAAAATGCTTTTAAGTGCAGCTTTAATGTGGGGCGTAGTGTGTTAAAG 29802 0.11667758977503095 No Hit
TAGAAACATTATTTTAGATTTTGTCTGGTAAGATGAAAGCATTGTTTTTA 29562 0.11573796755014647 No Hit
GCAATTTCGAGTTTTAAGATAGCTGGTTGTTCGAAAAATGCTTTTAAGTG 29346 0.11489230754775043 No Hit
ATTGTTTTTAAGCGTTAAAAAAAAGTACCTTTAGTATAATGGCCTTTCAA 28961 0.11338499689533157 No Hit
AAAAAATATTTAAAGGAACTCGGCAAAATTAAACCTCGCCTGTTTAACAA 27987 0.10957169669934202 No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGGGGTCGCCG 27629 0.10817009354722266 Illumina Single End PCR Primer 1 (97% over 41bp)
TGAAAGCATTGTTTTTAAGCGTTAAAAAAAAGTACCTTTAGTATAATGGC 26780 0.10484617992669382 No Hit
GTTAAAGATACTCACTAGACCAGGATGTTATAAGTGAAAAATTCGGCTAA 26751 0.10473264224118693 No Hit
AAAATATTTAAAGGAACTCGGCAAAATTAAACCTCGCCTGTTTAACAAAA 26307 0.10299434112515063 No Hit
CCGAAATAAAAAGATTTTTAGGAAAGCAGAATAATAGAGATGTTCGATGT 26202 0.10258325640176368 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CGCCG 1595800 3.9286137 122.65764 46
TTTTT 7089195 3.9156537 11.306798 35
TCGCC 1644025 3.4607248 87.64958 45
GGGGG 2432045 2.9389355 19.073074 40
CCTCC 1037625 2.7689376 6.341659 27
CTCGG 1661985 2.7597616 82.206726 37
CTTTT 3221910 2.6383784 13.396796 34
AAAAA 8396910 2.6138613 6.6447377 29
TTTCT 3164660 2.5914974 13.575185 37
AGCAG 2852695 2.5401874 14.659685 21
CTCCC 942035 2.5138524 6.0770855 28
AAGAG 4675990 2.504141 28.768145 7
TAGCA 3109050 2.3672051 12.413114 10
TTTTC 2857585 2.3400378 13.268261 36
GAAGA 4351710 2.330479 29.117437 6
CCACC 975285 2.320574 5.123802 8
TTTAA 5256315 2.308187 7.465813 26
ACTTT 3033630 2.2150192 12.038235 33
CGTCG 1324920 2.2000582 75.9973 12
ACCTC 1361000 2.1842666 5.5729094 26
TGAAG 3594920 2.1591525 9.969974 45
GAAAG 3915020 2.0966175 25.743599 23
ATCTC 1933210 2.0927157 52.932713 35
AAAGA 5125015 2.0925183 19.53878 24
GCGTC 1255075 2.084079 75.51261 11
GGTGG 1990925 2.0571744 41.249897 40
GGAAG 2896150 2.034311 37.452785 5
GTCGC 1224285 2.0329516 68.81541 44
TCTCG 1394875 1.9805124 67.77008 36
GCAGT 1974850 1.9722112 15.407587 22
AACTT 3009605 1.9593657 10.800413 32
CAGTT 2290855 1.9562063 13.462169 23
GGAAA 3559920 1.9064502 26.071594 22
TGGTC 1690320 1.8932025 40.33111 42
GGGGC 1227175 1.8799188 13.732955 42
AGAGC 2069770 1.8430305 46.57932 8
AGCGT 1831665 1.8292174 45.954197 10
TTCTA 2500060 1.8254303 11.477643 38
GAGGG 1980395 1.8245645 17.07997 27
CTAGC 1415490 1.7920092 19.044043 9
ATGAA 3907300 1.7892035 7.904406 44
CCTCG 838160 1.7643534 5.415825 12
GGTCG 1345535 1.7624849 54.555737 43
CGGTG 1345425 1.7623409 51.15019 39
TTAAA 4500935 1.7623148 7.1852317 27
TCTAA 2704275 1.7605842 10.353585 39
AAATG 3828615 1.7531729 7.6928296 42
AAAAC 3365890 1.7421604 8.644822 30
GAGCG 1485755 1.7352765 59.9152 9
AGGGA 2468050 1.7336054 32.866924 20
AAACT 2977535 1.7284387 9.686315 31
CTAAA 2955530 1.715665 9.399419 40
GATCT 1998535 1.7065886 41.743076 34
GGGGA 1841305 1.6964189 14.440153 1
GTTTA 2919520 1.6815592 9.254684 25
GGGAA 2385565 1.6756663 33.524494 21
GGAGG 1799060 1.6574981 5.934845 18
GTGTA 2446805 1.6481711 28.78809 16
GAAGT 2733445 1.6417401 9.552267 46
CGCCC 524955 1.6383137 7.499716 46
TCGGA 1632225 1.630044 52.26804 3
GGGCG 1056235 1.6180545 17.875359 43
AGAGG 2297790 1.6140115 13.216188 26
CGTGT 1437795 1.6103681 48.13593 15
GCAAT 2098605 1.5978608 12.0772505 12
TAAAT 4064925 1.5915977 6.9284697 16
AGCAA 2336770 1.5864094 10.942164 11
AGATC 2067240 1.5739799 37.18802 33
ATCGG 1568235 1.5661395 54.639297 2
CGGAA 1757790 1.5652274 47.14552 4
AGTTT 2714935 1.5637243 9.25395 24
GTCGT 1395710 1.5632316 47.178303 13
TGTAG 2309690 1.5558102 28.703114 17
CGGGG 1014890 1.5547178 20.732662 39
TCGTG 1367835 1.5320108 46.987694 14
GATCG 1526515 1.5244753 54.664032 1
CGCCT 722825 1.5215695 5.243436 15
GTGGT 1692955 1.4957514 32.242386 41
CTACT 1377390 1.491036 16.505934 6
GCGCC 593335 1.4606993 25.344564 45
ATAGC 1916640 1.459314 12.525346 19
TAAAA 4110120 1.4349157 6.0868187 28
GGAGA 2009600 1.4115814 6.582195 31
ACTAC 1428265 1.3785774 15.3758135 5
CTTTA 1874115 1.3683937 5.005464 1
GGACT 1336375 1.3345892 15.905746 3
AGTGT 1966535 1.3246605 22.682945 28
TCGGT 1180335 1.322006 43.39395 38
AATGA 2859070 1.3092054 7.4543843 43
GGGAC 1103505 1.2888306 18.007717 2
ACTAG 1684335 1.2824389 11.467359 8
GTAGA 2074205 1.2457925 27.638407 31
GAGTG 1529970 1.2052804 26.670607 27
AGAGT 1966125 1.1808785 20.544754 26
AGGGG 1280115 1.1793871 7.749598 20
AAATA 3351115 1.1699337 5.713176 17
GGGAG 1266385 1.1667376 6.868337 30
GGCGC 596180 1.157775 19.960775 44
TAGGG 1438620 1.1333165 37.813305 19
GTAGG 1434770 1.1302836 37.935387 18
AATAA 3167960 1.105991 5.697591 14
CAATA 1893025 1.0988882 9.1159115 13
ATAAA 3115240 1.0875854 5.654975 15
AGGGT 1342495 1.0575912 10.645992 28
TAGAT 2021655 1.0382427 23.14901 32
AATAG 2114720 0.9683579 7.218642 18
TCGGG 721330 0.94485325 16.93215 38
GGCGG 616670 0.9446815 9.5409975 13
TACTA 1393060 0.9069343 10.029182 7
TGGGC 672215 0.8805186 5.318015 42
GGGTC 671730 0.8798834 8.879125 42
GCGGG 572145 0.8764733 9.567563 14
GACTA 1150130 0.8756996 11.789883 4
GGGGT 791490 0.8178274 7.5689034 41
CGGCG 418895 0.8134895 11.774427 12
GGTAG 955850 0.7529999 10.012338 30
GCGGC 376600 0.73135304 11.80545 11
GGGTA 867895 0.6837107 10.067652 29
GTCGG 465070 0.6091843 6.1441436 13
AGCGG 482895 0.56399363 7.4702873 10
CGGGT 392430 0.51403487 8.640686 15
Produced by FastQC (version 0.10.1)