robertsmac:bsmap-2.42 sr320$ ./bsmap -a /Volumes/NGS\ Drive/NGS\ Raw\ Data/Oyster_BSseq/filtered_Unlabeled_NoIndex_L003_R1.fastq -d /Volumes/Bay3\ scratch/Consensus17_g1000.fa -o /Volumes/Bay3\ scratch/bsmapoutput

killed

robertsmac:bsmap-2.42 sr320$ ./bsmap -a /Volumes/NGS\ Drive/NGS\ Raw\ Data/Oyster_BSseq/filtered_Unlabeled_NoIndex_L003_R1.fastq -d /Volumes/Bay3\ scratch/Consensus17_g1000.fa -o /Volumes/Bay3\ scratch/bsmapoutput2.sam -p 3

BSMAP v2.42
Start at:  Tue Jan 31 16:19:57 2012

at 3am Wed file at 22GB.


complete

139700000 reads finished. 39378 secs passed
Total number of aligned reads: 67549086 (48%)
Done.
Finished at Wed Feb  1 03:16:15 2012
Total time consumed:  39378 secs
robertsmac:bsmap-2.42 sr320$ 



Ran consensus of BS de novo on Fosmid 17 >10000
- complete

outputconsen_17.sam


Running Raw BSseq on  Fosmid 17 >1000

Consensus17_g1000.fa  <temp link

megablast results from Consensus17_g1000.fa
@
http://www.evernote.com/shard/s10/sh/a5cefc4e-668b-415a-a062-c216e0503b29/c182a547dc2f78385b7cc48ea276d7f1

Complete


Imported into CLC with Reference..



Exported as tab-delimited file

bsmapoutput2.txt

Running Raw BSeq on MBD consensus, node 1

ConsensusOysterMBD_163774.fa  <-temp link

Complete


Attempting to upload to CLC Server to visualize..






SP annotation of ConsensusOysterMBD_163774.fa

MBD_SP_blastall copy.txt





Started bsmap of reads on Roberts_genomic_NCBI.fa (from crassostreome) node 1
exporting to bigfish Public.

Done











Mac's FILE







greenbird

robertsmac:bsmap-2.42 sr320$ ./bsmap -a /Volumes/NGS\ Drive/NGS\ Raw\ Data/Oyster_BSseq/filtered_Unlabeled_NoIndex_L003_R1.fastq -d /Volumes/Bay3\ scratch/Roberts_Cgigas_genomic_NCBI.fa -p 2 -o /Volumes/Bay3\ scratch/reads_Roberts_Cgigas_genomic_NCBI.BAM
done

fail on CLC import


robertsmac:bsmap-2.42 sr320$ ./bsmap -p 2 -a /Volumes/NGS\ Drive/NGS\ Raw\ Data/Oyster_BSseq/filtered_Unlabeled_NoIndex_L003_R1.fastq -d /Volumes/Bay3/Dropbox/Chrome\ Downloads/NCBI_Cg_genomic.fasta -o /Volumes/Bay3\ scratch/reads_NCBI_Cg_genomic.BAM
done