##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename fastq_quality_filter_out.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 47749573 Filtered Sequences 0 Sequence length 50 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.39996648765843 34.0 34.0 34.0 31.0 34.0 2 33.49444685086503 34.0 34.0 34.0 31.0 34.0 3 33.516678023487245 34.0 34.0 34.0 31.0 34.0 4 36.701487131623146 37.0 37.0 37.0 37.0 37.0 5 36.68859277547885 37.0 37.0 37.0 37.0 37.0 6 36.69417378873734 37.0 37.0 37.0 36.0 37.0 7 36.5318100750346 37.0 37.0 37.0 35.0 37.0 8 36.56289091841722 37.0 37.0 37.0 35.0 37.0 9 38.49448113389412 39.0 39.0 39.0 38.0 39.0 10 38.52019929057795 39.0 39.0 39.0 38.0 39.0 11 38.56147492669725 39.0 39.0 39.0 38.0 39.0 12 38.56083159110135 39.0 39.0 39.0 38.0 39.0 13 38.56277225767024 39.0 39.0 39.0 38.0 39.0 14 40.27247764079482 41.0 40.0 41.0 39.0 41.0 15 40.26701972811359 41.0 40.0 41.0 39.0 41.0 16 40.25715811113117 41.0 40.0 41.0 39.0 41.0 17 40.22745640050017 41.0 40.0 41.0 39.0 41.0 18 40.19755081370047 41.0 40.0 41.0 39.0 41.0 19 40.227703104276976 41.0 40.0 41.0 39.0 41.0 20 40.220715586294354 41.0 40.0 41.0 39.0 41.0 21 40.20531452710583 41.0 40.0 41.0 39.0 41.0 22 40.16708344177235 41.0 40.0 41.0 39.0 41.0 23 40.16254281478077 41.0 40.0 41.0 39.0 41.0 24 40.14835575597713 41.0 40.0 41.0 39.0 41.0 25 40.11495047296025 41.0 40.0 41.0 38.0 41.0 26 40.082058786159195 41.0 40.0 41.0 38.0 41.0 27 40.09892903126066 41.0 40.0 41.0 38.0 41.0 28 40.06619244532302 41.0 40.0 41.0 38.0 41.0 29 40.04050792244781 41.0 40.0 41.0 38.0 41.0 30 40.001672266262986 41.0 40.0 41.0 38.0 41.0 31 39.96583940132826 41.0 40.0 41.0 38.0 41.0 32 39.94181941689824 41.0 40.0 41.0 38.0 41.0 33 39.90551553204465 41.0 40.0 41.0 38.0 41.0 34 39.87387656848785 41.0 40.0 41.0 38.0 41.0 35 39.91846643319722 41.0 40.0 41.0 38.0 41.0 36 39.919057956811464 41.0 40.0 41.0 38.0 41.0 37 39.87297846621581 41.0 40.0 41.0 38.0 41.0 38 39.85751631747576 41.0 40.0 41.0 38.0 41.0 39 39.798285316603774 41.0 40.0 41.0 38.0 41.0 40 39.780547796731085 41.0 40.0 41.0 38.0 41.0 41 39.74233955976947 41.0 40.0 41.0 38.0 41.0 42 39.6832101304864 41.0 40.0 41.0 38.0 41.0 43 39.638517291034205 41.0 40.0 41.0 37.0 41.0 44 39.58603202587801 41.0 40.0 41.0 37.0 41.0 45 39.61298897897998 41.0 40.0 41.0 37.0 41.0 46 39.593790105725134 41.0 40.0 41.0 37.0 41.0 47 39.549400871082135 41.0 40.0 41.0 37.0 41.0 48 39.47009787919988 41.0 40.0 41.0 37.0 41.0 49 39.47154430051134 41.0 40.0 41.0 37.0 41.0 50 38.81748230502501 40.0 38.0 41.0 35.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 11.0 15 212.0 16 1347.0 17 4115.0 18 7171.0 19 11016.0 20 14838.0 21 17190.0 22 20449.0 23 23925.0 24 28935.0 25 35296.0 26 43974.0 27 56080.0 28 72391.0 29 93396.0 30 120069.0 31 158903.0 32 208496.0 33 282308.0 34 408143.0 35 655199.0 36 979138.0 37 1727809.0 38 4671418.0 39 3.8107744E7 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.522581196694677 19.962149190318414 18.585068813076088 33.93020079991082 2 23.369844584788225 21.079074361565496 37.65228644034157 17.89879461330471 3 24.06527907589875 26.954217998975615 29.16316550097736 19.817337424148274 4 23.624757859091222 34.23798365694286 22.12281772655852 20.0144407574074 5 21.54285651936615 37.49637300421514 25.338214019212273 15.622556457206436 6 18.214183821078358 36.58922771937667 27.517146593122416 17.679441866422554 7 14.336951243522114 24.713674402910367 45.197440823188096 15.751933530379421 8 19.256861207952582 24.83374458657463 33.12150456298321 22.787889642489578 9 19.837061160735406 24.498462007189048 33.145347708135525 22.51912912394002 10 18.4178149614029 39.55724378938426 26.688485779757652 15.336455469455194 11 22.680676118297434 31.075739672059477 23.3734132030877 22.87017100655539 12 20.19313136056735 27.002117903755913 30.75269385131465 22.052056884362088 13 18.188696682167187 29.533160432659784 32.81776153265287 19.460381352520155 14 19.149264434259965 29.74370681806935 32.44973939348107 18.65728935418962 15 20.140207327089605 29.488904539523315 30.58654158017287 19.784346553214206 16 19.594346948400982 29.643429481557877 31.263653394345535 19.498570175695605 17 21.072626136363564 29.75582420391487 29.302613868400456 19.868935791321107 18 19.887560041636394 29.464139920162218 30.227920572190246 20.42037946601114 19 19.67176753601545 30.83990510239746 30.238314382413428 19.250012979173654 20 20.696714921408827 29.60923441137369 30.311184143992243 19.382866523225246 21 20.252740270577917 31.01139982969062 29.399186459740694 19.33667343999076 22 20.449868734951828 30.104283864486074 30.2315813379106 19.214266062651493 23 19.58803275581124 30.3546169931195 30.23565257850578 19.82169767256348 24 20.107576668800785 30.58937301910532 30.11588606247851 19.187164249615385 25 20.788093749026824 29.357521165686656 30.245506069761085 19.60887901552544 26 20.050704956042225 29.986360296876374 30.692467134732283 19.270467612349123 27 19.932542642842062 28.933249308847227 30.522021631481394 20.61218641682932 28 19.84740470872902 29.45510319013743 30.857258137156535 19.84023396397702 29 20.4369764730671 30.838908234844318 29.104044553445537 19.620070738643044 30 20.021554957151135 31.39909753747955 29.25040816595365 19.328939339415662 31 20.61513932281656 31.30361396111333 28.639868674846582 19.44137804122353 32 20.772883141803174 30.388981698328486 28.675341243365676 20.162793916502668 33 20.8772086820546 29.49000612005473 29.086364814194255 20.546420383696415 34 21.078691112065023 29.435637047476842 29.018156874408067 20.467514966050064 35 20.17768829053194 30.111850424295938 29.94019025049711 19.77027103467501 36 20.442986998019858 30.449024120069097 28.996556681250325 20.111432200660726 37 19.669924587597883 30.12417304757888 29.89030498764879 20.315597377174452 38 20.629154107828356 30.280612142856228 29.728190448949142 19.362043300366267 39 20.09672840425191 30.372715584283867 29.38401564344879 20.146540368015437 40 19.884403992471306 30.292260414559102 29.31868731056506 20.504648282404535 41 20.44909595317219 29.47668244069952 30.10550272355315 19.968718882575136 42 20.962962328479883 30.992444686364003 28.281907358627063 19.76268562652906 43 20.540340748178 30.565563382106053 28.573128811015756 20.32096705870019 44 20.714057484870075 29.227147224960525 29.970427170102653 20.08836812006675 45 20.632678746676962 29.116088640206268 30.26176171250788 19.98947090060889 46 21.00569988343142 29.91203293064003 29.163827287000032 19.91843989892852 47 20.9648932357992 30.062696058035954 29.39790058436753 19.57451012179732 48 20.81365628128235 29.07263903700249 29.36919456850431 20.744510113210858 49 21.221900769667617 29.243453129936892 29.710525369514823 19.824120730880672 50 20.562763985344958 29.872057704055283 29.395257628795967 20.169920681803795 >>END_MODULE >>Per base GC content fail #Base %GC 1 61.4527819966055 2 41.268639198092934 3 43.88261650004703 4 43.639198616498625 5 37.16541297657259 6 35.893625687500915 7 30.088884773901537 8 42.044750850442156 9 42.35619028467543 10 33.75427043085809 11 45.55084712485282 12 42.24518824492944 13 37.64907803468734 14 37.806553788449584 15 39.92455388030381 16 39.09291712409659 17 40.941561927684674 18 40.30793950764753 19 38.921780515189106 20 40.07958144463407 21 39.58941371056868 22 39.664134797603325 23 39.40973042837472 24 39.294740918416174 25 40.39697276455227 26 39.32117256839135 27 40.54472905967138 28 39.687638672706036 29 40.05704721171014 30 39.350494296566794 31 40.0565173640401 32 40.93567705830584 33 41.423629065751015 34 41.546206078115084 35 39.94795932520695 36 40.55441919868058 37 39.98552196477234 38 39.99119740819462 39 40.24326877226734 40 40.389052274875844 41 40.41781483574733 42 40.72564795500894 43 40.86130780687819 44 40.80242560493682 45 40.62214964728585 46 40.92413978235994 47 40.53940335759651 48 41.5581663944932 49 41.046021500548285 50 40.73268466714875 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 69326.0 1 42657.5 2 15989.0 3 12071.0 4 8153.0 5 7844.5 6 7536.0 7 7986.0 8 8436.0 9 11141.5 10 13847.0 11 22107.0 12 30367.0 13 48942.5 14 67518.0 15 96866.5 16 126215.0 17 217121.0 18 308027.0 19 459058.0 20 610089.0 21 630137.5 22 650186.0 23 931789.5 24 1213393.0 25 1514532.5 26 1815672.0 27 2001140.5 28 2186609.0 29 2582725.0 30 2978841.0 31 2937550.0 32 2896259.0 33 2874806.0 34 2853353.0 35 2711955.5 36 2570558.0 37 2784287.0 38 2998016.0 39 3050689.0 40 3103362.0 41 3085890.0 42 3068418.0 43 3149170.0 44 3229922.0 45 3259992.0 46 3290062.0 47 3091131.0 48 2892200.0 49 2763198.5 50 2634197.0 51 2378177.0 52 2122157.0 53 1907901.5 54 1693646.0 55 1522018.0 56 1350390.0 57 1171120.5 58 991851.0 59 855841.5 60 719832.0 61 596259.0 62 472686.0 63 384016.0 64 295346.0 65 239989.0 66 184632.0 67 146926.5 68 109221.0 69 87863.5 70 66506.0 71 55237.0 72 43968.0 73 35059.5 74 26151.0 75 20895.5 76 15640.0 77 11784.5 78 7929.0 79 5126.0 80 2323.0 81 1365.0 82 407.0 83 287.5 84 168.0 85 123.0 86 78.0 87 63.0 88 48.0 89 31.5 90 15.0 91 13.0 92 11.0 93 10.5 94 10.0 95 7.0 96 4.0 97 2.5 98 1.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 4.7749573E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Duplicate Percentage 84.2368219326329 #Duplication Level Relative count 1 100.0 2 38.63111924785934 3 21.498178766901233 4 14.625169250719912 5 11.57198161603448 6 9.306405782176707 7 7.952400022884604 8 6.829147357781719 9 5.885157427007647 10++ 165.11051356866335 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGACTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGT 300198 0.6286925330201383 No Hit GGACTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTAT 158824 0.33261868121836397 No Hit ATAATAATCAATCAAAGAATATCTTAGACAGGTGAACGATTTGCTAAGCC 151789 0.31788556517562994 No Hit GGGACTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTA 142154 0.2977073742628023 No Hit GGCCATTATACTAAAGGTACTTTTTTTTAACGCTTAAAAACAATGCTTTC 135326 0.2834077699501103 No Hit GGGAGGTGCTAATACAGCAAAATCTCTTGAAAGGCCATTATACTAAAGGT 128931 0.2700149800292455 No Hit CAGCATAATAATCAATCAAAGAATATCTTAGACAGGTGAACGATTTGCTA 125286 0.26238140391328735 No Hit CCCACATTAAAGCTGCACTTAAAAGCATTTTTCGAACAACCAGCTATCTT 106223 0.2224585338176741 No Hit GTCCTTTCGTACATGCCGAGATAAGTTTATGTAAAGCAGATAGAAACCAA 97903 0.2050342942333746 No Hit CCACATTAAAGCTGCACTTAAAAGCATTTTTCGAACAACCAGCTATCTTA 90511 0.18955352752578541 No Hit ATTAAATTATCATTCTGCAATTTTTTTCTGGATTTACTCTTTCCAGTCTC 88903 0.18618595814458905 No Hit CTAGTGAGTATCTTTAACACACTACGCCCCACATTAAAGCTGCACTTAAA 82467 0.172707303581542 No Hit ATTTTTTAGAAATTATTCTTTTACTATTAGAAAGACAAGTTGTTAAATAC 80417 0.1684140714724297 No Hit GAACGATTTGCTAAGCCTCATTTTTTAGAAATTATTCTTTTACTATTAGA 77135 0.16154071157871924 No Hit CCACAATCAAAAGGCAAGGAATTTCGCTACCTTAGCACCCTCACGCTAGG 68106 0.1426316419625365 No Hit CTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGG 67826 0.14204524928421872 No Hit AATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGGAGCATTAG 58940 0.12343565878589113 No Hit GTCAAATCCGGAAAGAGTTAGTTATTCAGAAAGGTTAGACTTGCTTTCTA 57917 0.12129323125046583 No Hit CTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGGAGC 56768 0.11888692700979756 No Hit CCAGCATAATAATCAATCAAAGAATATCTTAGACAGGTGAACGATTTGCT 55798 0.11685549523133955 No Hit CTTAAAACTCGAAATTGCATATCACATCCTTAGCAAAGTTTATTCAAGAA 55557 0.11635077867607319 No Hit CTCATTTTTTAGAAATTATTCTTTTACTATTAGAAAGACAAGTTGTTAAA 55354 0.1159256439842928 No Hit CTTTCCTAAAAATCTTTTTATTTCGGGAGGTGCTAATACAGCAAAATCTC 54185 0.1134774545523161 No Hit CCTGATTCAACATCGAGGTGCCAATCCCTTTAGCCAATACGAACTCTACT 52180 0.10927846412364776 No Hit ATTATACTAAAGGTACTTTTTTTTAACGCTTAAAAACAATGCTTTCATCT 51663 0.1081957319283253 No Hit GGAGGTGCTAATACAGCAAAATCTCTTGAAAGGCCATTATACTAAAGGTA 51547 0.10795279781873651 No Hit CTGCTTTCCTAAAAATCTTTTTATTTCGGGAGGTGCTAATACAGCAAAAT 50283 0.10530565372804486 No Hit TAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGGAGCA 49916 0.10453706046753548 No Hit CTAAAAATCTTTTTATTTCGGGAGGTGCTAATACAGCAAAATCTCTTGAA 48940 0.10249306313168498 No Hit >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position TTTTT 19774120 3.8650827 7.0923777 21 AAAAA 13824470 2.698305 5.3238044 29 AGCAG 4076185 2.4938018 11.579457 21 GGAGG 2874570 2.489291 8.300537 2 CAGCA 3800895 2.3843186 7.50487 1 CCAGC 2467170 2.3031006 7.056272 40 GCTGC 2381500 2.1687918 7.1271963 12 CTTTT 7346865 2.136364 5.521807 34 TTTCT 7344080 2.135554 5.8702555 37 CTTTC 4910330 2.1242003 5.8521504 4 GAAAG 4930385 2.0269988 5.186816 29 TGAAG 4792875 1.9710269 7.2995367 45 TTTTC 6767385 1.9678596 5.5676208 36 GCCCC 1411750 1.9611329 6.8153515 26 AGGTG 3258705 1.9449416 6.2915573 34 CAGCT 3019455 1.8946577 5.290381 40 CCCCA 1967695 1.8833973 5.1714644 27 CTGCA 2987685 1.8747227 5.6087537 13 GGCCA 2052985 1.8690859 9.323628 1 AGCTG 3016830 1.8462169 5.8432474 10 AGCAA 4259200 1.7954404 7.20144 11 GGTGC 2020945 1.7949462 7.270715 5 GGGAG 2067335 1.7902498 8.684742 1 AAAGG 4348945 1.7879547 5.0919733 30 CTGGT 2916725 1.7854643 5.760015 1 CCCAC 1860195 1.7805029 9.477313 1 TAGCA 4160910 1.7545072 6.9987626 10 CCACA 2724890 1.7526602 6.776251 1 AAAAC 5867375 1.7042047 6.0041885 30 ACCAG 2714205 1.7026331 5.2240915 39 GGGGA 1965185 1.7017909 15.441489 1 GCCTC 1803210 1.6837745 5.965769 15 GACAG 2679345 1.639218 6.226292 31 GCAGT 2659780 1.6277119 9.640218 22 TTGCT 3814325 1.609282 5.243939 41 CGCCC 1157165 1.6074761 6.3038244 25 ACAGC 2555695 1.6031989 5.0877466 14 ACAGG 2609610 1.5965542 6.23572 32 AAATG 5569005 1.5780073 5.2512116 42 CTAAA 5427755 1.5769647 5.0644903 40 ACTTT 5244675 1.5246422 5.2575336 33 GAAGT 3699745 1.521487 6.89717 46 ATGAA 5279710 1.4960341 5.245607 44 AGGCC 1642530 1.495398 7.152387 32 CAGTT 3542065 1.4939883 7.119596 23 GGTGA 2449945 1.4622372 6.1514 35 CAGGT 2374925 1.4533886 6.1030784 33 ATCAA 4993785 1.4508806 5.5637302 11 GGGAC 1621310 1.4395912 14.665741 2 GTGCT 2276195 1.3933659 5.291031 6 GCCAT 2218560 1.3921094 5.679182 2 GCAAT 3292790 1.3884519 6.9619093 12 AGTTT 4820700 1.3667501 6.267488 24 AACTT 4605595 1.3384783 5.12763 32 AAGGC 2171845 1.3287305 5.645146 31 AAACT 4554015 1.3231108 5.1925917 31 GTTTA 4659500 1.3210473 6.1187468 25 GAGGT 2165095 1.292226 5.4389467 3 AATCA 4411950 1.2818356 5.6707807 10 AATGA 4341630 1.2302241 5.111223 43 GGACT 1970355 1.205803 10.79102 3 AAGCC 1807285 1.1337181 5.4911537 46 TCTAA 3813380 1.1082448 5.073689 39 CTAGC 1708590 1.0721118 9.512642 9 ATAGC 2507680 1.0573992 7.1164026 19 ACTAC 2111665 0.9129819 7.121857 5 CTACT 2074245 0.8970591 6.865069 6 GAACG 1447690 0.88569385 5.3666735 38 ACTAG 1992865 0.84031993 6.4206476 8 GACTA 1498410 0.631826 6.8684177 4 >>END_MODULE