FastQCFastQC Report
Mon 24 Mar 2014
fastq_quality_filter_out.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename fastq_quality_filter_out.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 47749573
Filtered Sequences 0
Sequence length 50
%GC 40

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GGGGACTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGT 300198 0.6286925330201383 No Hit
GGACTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTAT 158824 0.33261868121836397 No Hit
ATAATAATCAATCAAAGAATATCTTAGACAGGTGAACGATTTGCTAAGCC 151789 0.31788556517562994 No Hit
GGGACTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTA 142154 0.2977073742628023 No Hit
GGCCATTATACTAAAGGTACTTTTTTTTAACGCTTAAAAACAATGCTTTC 135326 0.2834077699501103 No Hit
GGGAGGTGCTAATACAGCAAAATCTCTTGAAAGGCCATTATACTAAAGGT 128931 0.2700149800292455 No Hit
CAGCATAATAATCAATCAAAGAATATCTTAGACAGGTGAACGATTTGCTA 125286 0.26238140391328735 No Hit
CCCACATTAAAGCTGCACTTAAAAGCATTTTTCGAACAACCAGCTATCTT 106223 0.2224585338176741 No Hit
GTCCTTTCGTACATGCCGAGATAAGTTTATGTAAAGCAGATAGAAACCAA 97903 0.2050342942333746 No Hit
CCACATTAAAGCTGCACTTAAAAGCATTTTTCGAACAACCAGCTATCTTA 90511 0.18955352752578541 No Hit
ATTAAATTATCATTCTGCAATTTTTTTCTGGATTTACTCTTTCCAGTCTC 88903 0.18618595814458905 No Hit
CTAGTGAGTATCTTTAACACACTACGCCCCACATTAAAGCTGCACTTAAA 82467 0.172707303581542 No Hit
ATTTTTTAGAAATTATTCTTTTACTATTAGAAAGACAAGTTGTTAAATAC 80417 0.1684140714724297 No Hit
GAACGATTTGCTAAGCCTCATTTTTTAGAAATTATTCTTTTACTATTAGA 77135 0.16154071157871924 No Hit
CCACAATCAAAAGGCAAGGAATTTCGCTACCTTAGCACCCTCACGCTAGG 68106 0.1426316419625365 No Hit
CTACTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGG 67826 0.14204524928421872 No Hit
AATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGGAGCATTAG 58940 0.12343565878589113 No Hit
GTCAAATCCGGAAAGAGTTAGTTATTCAGAAAGGTTAGACTTGCTTTCTA 57917 0.12129323125046583 No Hit
CTAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGGAGC 56768 0.11888692700979756 No Hit
CCAGCATAATAATCAATCAAAGAATATCTTAGACAGGTGAACGATTTGCT 55798 0.11685549523133955 No Hit
CTTAAAACTCGAAATTGCATATCACATCCTTAGCAAAGTTTATTCAAGAA 55557 0.11635077867607319 No Hit
CTCATTTTTTAGAAATTATTCTTTTACTATTAGAAAGACAAGTTGTTAAA 55354 0.1159256439842928 No Hit
CTTTCCTAAAAATCTTTTTATTTCGGGAGGTGCTAATACAGCAAAATCTC 54185 0.1134774545523161 No Hit
CCTGATTCAACATCGAGGTGCCAATCCCTTTAGCCAATACGAACTCTACT 52180 0.10927846412364776 No Hit
ATTATACTAAAGGTACTTTTTTTTAACGCTTAAAAACAATGCTTTCATCT 51663 0.1081957319283253 No Hit
GGAGGTGCTAATACAGCAAAATCTCTTGAAAGGCCATTATACTAAAGGTA 51547 0.10795279781873651 No Hit
CTGCTTTCCTAAAAATCTTTTTATTTCGGGAGGTGCTAATACAGCAAAAT 50283 0.10530565372804486 No Hit
TAGCAATAAATAGCAGTTTAAAAACTTTTTCTAAATGAAGTATAGGAGCA 49916 0.10453706046753548 No Hit
CTAAAAATCTTTTTATTTCGGGAGGTGCTAATACAGCAAAATCTCTTGAA 48940 0.10249306313168498 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 19774120 3.8650827 7.0923777 21
AAAAA 13824470 2.698305 5.3238044 29
AGCAG 4076185 2.4938018 11.579457 21
GGAGG 2874570 2.489291 8.300537 2
CAGCA 3800895 2.3843186 7.50487 1
CCAGC 2467170 2.3031006 7.056272 40
GCTGC 2381500 2.1687918 7.1271963 12
CTTTT 7346865 2.136364 5.521807 34
TTTCT 7344080 2.135554 5.8702555 37
CTTTC 4910330 2.1242003 5.8521504 4
GAAAG 4930385 2.0269988 5.186816 29
TGAAG 4792875 1.9710269 7.2995367 45
TTTTC 6767385 1.9678596 5.5676208 36
GCCCC 1411750 1.9611329 6.8153515 26
AGGTG 3258705 1.9449416 6.2915573 34
CAGCT 3019455 1.8946577 5.290381 40
CCCCA 1967695 1.8833973 5.1714644 27
CTGCA 2987685 1.8747227 5.6087537 13
GGCCA 2052985 1.8690859 9.323628 1
AGCTG 3016830 1.8462169 5.8432474 10
AGCAA 4259200 1.7954404 7.20144 11
GGTGC 2020945 1.7949462 7.270715 5
GGGAG 2067335 1.7902498 8.684742 1
AAAGG 4348945 1.7879547 5.0919733 30
CTGGT 2916725 1.7854643 5.760015 1
CCCAC 1860195 1.7805029 9.477313 1
TAGCA 4160910 1.7545072 6.9987626 10
CCACA 2724890 1.7526602 6.776251 1
AAAAC 5867375 1.7042047 6.0041885 30
ACCAG 2714205 1.7026331 5.2240915 39
GGGGA 1965185 1.7017909 15.441489 1
GCCTC 1803210 1.6837745 5.965769 15
GACAG 2679345 1.639218 6.226292 31
GCAGT 2659780 1.6277119 9.640218 22
TTGCT 3814325 1.609282 5.243939 41
CGCCC 1157165 1.6074761 6.3038244 25
ACAGC 2555695 1.6031989 5.0877466 14
ACAGG 2609610 1.5965542 6.23572 32
AAATG 5569005 1.5780073 5.2512116 42
CTAAA 5427755 1.5769647 5.0644903 40
ACTTT 5244675 1.5246422 5.2575336 33
GAAGT 3699745 1.521487 6.89717 46
ATGAA 5279710 1.4960341 5.245607 44
AGGCC 1642530 1.495398 7.152387 32
CAGTT 3542065 1.4939883 7.119596 23
GGTGA 2449945 1.4622372 6.1514 35
CAGGT 2374925 1.4533886 6.1030784 33
ATCAA 4993785 1.4508806 5.5637302 11
GGGAC 1621310 1.4395912 14.665741 2
GTGCT 2276195 1.3933659 5.291031 6
GCCAT 2218560 1.3921094 5.679182 2
GCAAT 3292790 1.3884519 6.9619093 12
AGTTT 4820700 1.3667501 6.267488 24
AACTT 4605595 1.3384783 5.12763 32
AAGGC 2171845 1.3287305 5.645146 31
AAACT 4554015 1.3231108 5.1925917 31
GTTTA 4659500 1.3210473 6.1187468 25
GAGGT 2165095 1.292226 5.4389467 3
AATCA 4411950 1.2818356 5.6707807 10
AATGA 4341630 1.2302241 5.111223 43
GGACT 1970355 1.205803 10.79102 3
AAGCC 1807285 1.1337181 5.4911537 46
TCTAA 3813380 1.1082448 5.073689 39
CTAGC 1708590 1.0721118 9.512642 9
ATAGC 2507680 1.0573992 7.1164026 19
ACTAC 2111665 0.9129819 7.121857 5
CTACT 2074245 0.8970591 6.865069 6
GAACG 1447690 0.88569385 5.3666735 38
ACTAG 1992865 0.84031993 6.4206476 8
GACTA 1498410 0.631826 6.8684177 4