##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename filtered_174gm_A_BiGosperm_L006_R1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 171582441 Filtered Sequences 0 Sequence length 76 %GC 18 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.900386800068894 31.0 31.0 34.0 30.0 34.0 2 32.118850995947774 33.0 31.0 34.0 30.0 34.0 3 32.38516902787273 34.0 31.0 34.0 31.0 34.0 4 36.05814193423207 37.0 35.0 37.0 35.0 37.0 5 35.97134243474249 37.0 35.0 37.0 35.0 37.0 6 35.983762371115816 37.0 35.0 37.0 35.0 37.0 7 35.99434192686418 37.0 35.0 37.0 35.0 37.0 8 35.95779728999193 37.0 35.0 37.0 35.0 37.0 9 37.77219312901604 39.0 37.0 39.0 35.0 39.0 10-14 37.83893168299197 39.2 37.2 39.4 35.0 39.4 15-19 38.41347795605729 40.0 38.0 41.0 34.2 41.0 20-24 38.11982662375109 39.2 37.2 41.0 33.6 41.0 25-29 37.91570070156537 39.0 37.0 40.8 33.4 41.0 30-34 37.423803619858745 39.0 36.8 40.0 32.4 41.0 35-39 37.04867383020853 39.0 36.2 40.0 31.4 41.0 40-44 36.63360547714787 38.0 35.2 40.0 30.0 41.0 45-49 36.263145504498326 38.0 35.0 40.0 30.0 41.0 50-54 35.6704404887211 38.0 34.0 40.0 30.0 40.8 55-59 35.18432796278962 37.4 33.6 39.2 28.4 40.0 60-64 34.26948897061093 36.8 32.8 39.0 26.8 40.0 65-69 33.236008924712756 35.6 31.4 38.4 25.2 40.0 70-74 31.658753966555356 34.4 30.4 37.6 20.8 39.0 75-76 28.394403839376547 31.5 27.0 35.5 2.0 37.5 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9152.0 3 2490.0 4 5415.0 5 6800.0 6 9538.0 7 11612.0 8 14255.0 9 20011.0 10 32379.0 11 51952.0 12 76855.0 13 98434.0 14 110257.0 15 127737.0 16 150250.0 17 176255.0 18 210138.0 19 248455.0 20 296095.0 21 357043.0 22 432947.0 23 533975.0 24 671033.0 25 859882.0 26 1118902.0 27 1471460.0 28 1943725.0 29 2554349.0 30 3330643.0 31 4315301.0 32 5625832.0 33 7509781.0 34 1.0503475E7 35 1.6033089E7 36 2.773703E7 37 4.5531182E7 38 3.5931551E7 39 3462850.0 40 311.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 16.8049885665819 32.4176893392011 50.440971517084506 0.3363505771324883 2 17.03843166661516 34.15779058439456 48.550198157177476 0.25357959181280226 3 17.55016820165182 34.16586724045965 48.01376499824944 0.2701995596390892 4 18.10897771293509 33.43965481875853 48.020077415730434 0.43129005257595093 5 17.920640842264273 33.295883697096954 48.49463005366616 0.28884540697261674 6 17.786099103229333 33.57788283242806 48.338582617553506 0.29743544678910355 7 17.50198203556272 33.73313939507365 48.48290158082085 0.28197698854278447 8 17.175058722937738 33.748814075911184 48.789917261988364 0.2862099391627142 9 17.12449935363724 33.23631058495082 49.34463194867359 0.2945581127383542 10-14 16.933476393726686 33.3675501339541 49.36147033027904 0.337503142040181 15-19 17.275291639867508 32.96599359858299 49.39059778432907 0.3681169772204348 20-24 17.25922626764109 32.91150387990997 49.51412162417098 0.31514822827795774 25-29 17.4082662082079 32.92884342846263 49.31969367432594 0.3431966890035404 30-34 17.44957662696957 32.965932982165874 49.230407829862735 0.3540825610018161 35-39 17.568297630269342 32.92652582866541 49.14013531839812 0.36504122266711786 40-44 17.672815463796418 33.00424329172718 48.8621791637891 0.4607620806873016 45-49 17.852494695298763 33.040031940018764 48.47596978800997 0.6315035766725057 50-54 18.140846190435933 33.030753960718236 47.844899148553935 0.9835007002918995 55-59 18.31571716826459 33.10318820847232 47.18475135089456 1.3963432723685323 60-64 18.623983035365825 33.08771805399012 46.261370088890416 2.0269288217536348 65-69 18.910916753656128 33.2035398817773 45.109316262344436 2.7762271022221325 70-74 19.108964810233946 33.166250195683425 43.94872162429044 3.776063369792183 75-76 19.197196165475283 32.853936889455206 43.48872701036041 4.460139934709105 >>END_MODULE >>Per base GC content pass #Base %GC 1 17.14133914371439 2 17.292011258427962 3 17.82036776129091 4 18.54026776551104 5 18.20948624923689 6 18.083534550018438 7 17.783959024105503 8 17.461268662100455 9 17.419057466375595 10-14 17.270979535766866 15-19 17.643408617087943 20-24 17.57437449591905 25-29 17.751462897211436 30-34 17.803659187971384 35-39 17.93333885293646 40-44 18.13357754448372 45-49 18.483998271971267 50-54 19.124346890727832 55-59 19.712060440633124 60-64 20.65091185711946 65-69 21.68714385587826 70-74 22.885028180026126 75-76 23.657336100184388 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12811.0 1 17965.0 2 46816.0 3 149359.5 4 228206.0 5 433664.5 6 1070886.5 7 2240465.0 8 2978280.0 9 4031159.5 10 6345670.0 11 8900422.5 12 1.0193544E7 13 1.13144545E7 14 1.32136505E7 15 1.4335745E7 16 1.4610633E7 17 1.4541712E7 18 1.4135996E7 19 1.30775835E7 20 1.1649008E7 21 1.0873129E7 22 1.0081672E7 23 8549061.5 24 7144953.0 25 6481998.0 26 5912373.5 27 4866630.0 28 3998482.0 29 3606453.0 30 3286909.5 31 2702442.0 32 2205338.0 33 1973158.0 34 1764347.0 35 1378668.5 36 1054362.0 37 906923.0 38 788744.0 39 580117.5 40 430223.5 41 351308.5 42 331840.0 43 281645.5 44 189081.5 45 121270.0 46 95828.0 47 80657.0 48 57213.0 49 44250.0 50 39560.0 51 35117.5 52 26025.0 53 18510.5 54 15646.0 55 13545.0 56 9936.0 57 7231.5 58 6035.0 59 5248.5 60 3864.5 61 2863.0 62 2459.0 63 2114.0 64 1545.0 65 1181.5 66 901.0 67 760.0 68 703.5 69 563.5 70 457.0 71 434.0 72 399.5 73 352.0 74 300.5 75 262.0 76 257.5 77 221.0 78 188.0 79 187.0 80 163.5 81 142.5 82 120.0 83 95.0 84 83.0 85 59.0 86 43.5 87 40.0 88 35.0 89 23.0 90 18.0 91 13.5 92 7.0 93 13.0 94 22.5 95 50.0 96 74.0 97 99.5 98 167.0 99 166.5 100 124.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09729900042627322 2 0.006447629451780558 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 2.641295912091611E-4 15-19 8.700190947860452E-4 20-24 0.001520668423175073 25-29 0.018315976749625564 30-34 0.010748419181191158 35-39 0.01833497636276197 40-44 0.013233871640746736 45-49 0.03055254354377672 50-54 0.02637659176325624 55-59 0.03515499584249416 60-64 0.01862498272769065 65-69 0.092218177499876 70-74 0.24672559589008297 75-76 0.40732023389269767 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1.71582441E8 >>END_MODULE >>Sequence Duplication Levels warn #Total Duplicate Percentage 20.39278089271106 #Duplication Level Relative count 1 100.0 2 13.025497064297218 3 2.805366351895831 4 1.3265491550063602 5 0.9361664797257923 6 0.7011849336098805 7 0.5357579251442787 8 0.44991133362993224 9 0.39852870221258624 10++ 5.144529316297693 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content fail #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position CCCCC 40100 21939.352 28547.258 63 CACAC 13316205 7777.7886 43682.53 72 ACTCC 4301950 1734.989 12422.333 72 CTCCA 3862030 1557.568 11791.825 72 AGCAC 13804235 488.1336 2315.5806 72 GCACA 12943395 457.69327 2273.3809 72 ACACG 11005635 389.17188 2084.1794 72 CACGT 10216710 249.45554 1404.7568 72 ACGTC 9417450 229.94046 1310.0906 72 CCCCG 5705 188.96692 825.6892 71 CCGTC 242615 181.30159 11116.82 52 CGTCT 8834680 148.94594 856.44324 72 GCCCC 4075 134.97636 706.0809 72 ACCCC 7065 126.296036 729.7824 71 CTCCC 9330 115.16351 833.9638 72 CACCC 6325 113.06757 923.60425 72 CCCGC 2885 95.55996 382.9591 72 TCCAG 3560105 86.92503 681.68256 72 CCCAC 4540 81.15839 768.67584 70 CCAGT 3169775 77.39457 626.24664 72 CCGCC 2270 75.18929 250.29639 52 CCCCA 4110 73.47158 602.2543 69 CACGC 67030 72.54333 455.931 72 CGCCC 2090 69.22714 227.3637 71 CAGTC 2813475 68.695 556.9519 72 CGCAC 62895 68.06821 405.45657 71 AACTC 4959685 65.35572 459.19278 72 CCACC 3050 54.522694 568.32605 71 GTCAC 2230350 54.457176 456.7634 72 CGCGC 23830 47.786457 91.53153 69 CGGAA 21237070 45.464417 177.26395 72 CCTCC 3655 45.114967 352.31625 72 GATCG 25352225 37.475628 131.96066 72 ATCGG 24454490 36.148598 129.62354 72 AGAGC 16268745 34.828205 153.56142 72 TCGGA 23039310 34.05668 128.91801 72 CACTG 1334420 32.581764 361.30966 33 GAGCA 15157820 32.449932 150.33292 72 TCACA 1972045 25.98641 225.06862 72 GCACC 20515 22.20239 143.11383 72 ACACT 1545945 20.371523 194.71552 32 AGATC 25529100 20.366468 70.210495 71 CTCCG 27075 20.232635 198.44661 72 CGGCG 157945 19.175089 23.240093 24 CGCGG 154845 18.798738 22.26944 4 CCCCT 1500 18.515034 205.14618 72 TCGCG 380875 17.231266 20.177341 3 CCACG 15570 16.850657 94.61957 71 CGTCG 370915 16.780663 21.280561 71 GCCAC 15490 16.764076 105.57577 72 GCTCC 22145 16.548538 130.66724 71 CACCG 14545 15.741349 89.536705 71 CGCGA 237545 15.564128 18.051582 4 ACGCG 229995 15.069447 28.197424 70 CCGGC 7350 14.739004 355.74014 52 GCGCG 119775 14.541114 17.371824 70 CGCGT 313275 14.172955 22.669844 71 TCCCC 1100 13.577692 98.11339 72 CGACG 206540 13.532658 16.584127 1 GGCCC 6555 13.144787 63.753036 71 CCCAG 11160 12.077928 79.761955 71 CCCGG 5515 11.059267 60.85517 71 TGCCG 237970 10.766063 684.3324 50 GCCGT 235075 10.635089 680.54535 51 CGTCC 14005 10.465671 59.945377 70 ACGCC 7965 8.620134 60.212456 71 GTCTG 8181415 8.350587 50.136734 72 GGCAC 120155 7.872647 43.46342 72 CCCTC 630 7.7763143 57.976093 72 GCACG 113940 7.465435 42.702454 71 ACACC 12405 7.2455664 40.725864 71 CGGAC 103605 6.788278 8.365942 4 ACCCG 6215 6.726194 44.96385 71 CGCTC 8925 6.669483 393.2453 2 CGCCG 3300 6.6175127 54.83971 50 AGCGC 98975 6.484918 26.53731 72 CACCA 11055 6.4570537 34.39819 72 ACCAC 11015 6.4336905 36.72346 70 GGGGG 13331345 5.9320793 6.657552 43 CGGCC 2955 5.9256816 34.7809 70 CTCAC 14560 5.872091 46.487995 70 ATGCC 239535 5.8485885 366.9148 49 TCTCG 337120 5.683585 254.03279 43 GCGCC 2825 5.664992 23.18479 72 CCAGC 5205 5.633119 47.704605 72 CCACA 9330 5.449507 31.863241 71 CACAT 412355 5.433764 35.75541 72 CTGAA 6798260 5.4234796 33.904675 72 CACTC 13420 5.4123254 36.37895 69 GCGTC 118940 5.3809958 13.207432 72 CCGCG 2660 5.3341165 15.213793 71 CTCGT 314980 5.310322 254.54828 44 TGAAC 6403715 5.108721 31.366726 72 GACCC 4700 5.086583 40.275208 72 GCCCG 2410 4.8327894 18.836124 71 GCCCA 4370 4.7294393 29.326605 72 AACCC 8045 4.6989594 36.297474 72 CCCGT 6245 4.6667705 39.68932 72 CGCCA 4305 4.659093 33.23682 72 GCGGC 38065 4.621228 6.2907033 52 CGGTC 100400 4.5422225 10.395113 71 GCCGC 2260 4.5319934 16.596373 51 CGGGC 37250 4.522284 6.025849 4 GAACT 5667620 4.5214834 30.590212 72 CATAC 335390 4.4195657 25.619156 72 GCGCA 65035 4.2611423 22.03946 72 TCTGA 7473960 4.1170554 25.248346 72 ACGGC 61110 4.0039735 10.771164 72 TCGGC 88435 4.000911 6.593453 58 TCTGC 230445 3.8851264 251.40291 58 CTGCT 229060 3.8617764 251.28369 59 CGGGG 522530 3.8405504 4.654858 1 GGAAG 28730035 3.7236078 11.735041 72 TTCGC 211995 3.5740736 4.2873855 2 ATCTC 382705 3.4821656 136.56407 42 CGTTC 206335 3.4786503 4.111262 4 GTCGC 75605 3.4204657 7.110437 72 TCCCA 8245 3.3252327 26.228666 72 GGCGC 27155 3.2967148 6.711125 72 TCGTC 195240 3.2915971 3.9254644 55 CGAGC 48695 3.1905332 3.8827689 70 AGCCC 2920 3.1601748 19.94049 71 TACAC 232525 3.0640733 20.063023 72 CTTCC 10000 2.784756 145.13197 6 CGATC 108165 2.6410027 15.605688 10 CGCCT 3445 2.574383 20.249653 72 CGGAG 645255 2.5595348 6.0418177 71 CAGCC 2365 2.559525 16.815718 70 CACGG 37940 2.485858 16.617031 71 GAAGA 34810900 2.4349523 6.7620263 72 TCTCC 8700 2.4227376 21.93214 71 CACAG 64795 2.2912257 22.920343 72 CTCCT 7795 2.1707172 39.94385 72 GACAC 59770 2.113536 13.133842 72 AAGAG 30135635 2.1079268 6.1319585 72 ACCTC 5210 2.1012082 16.173044 71 CCGAC 1915 2.0725117 40.111656 52 AGTCA 2595305 2.0704687 17.096888 72 ACCGC 1685 1.8235941 10.166557 72 TCCCG 2350 1.7561105 14.03976 72 CACCT 4005 1.6152282 14.425766 72 CTTCT 243255 1.5282795 94.333855 56 TCTTC 237100 1.48961 93.304665 55 ACCCA 2520 1.4718928 13.717069 72 TCGTA 2643455 1.4561558 9.7288065 45 ACTCT 158535 1.442482 14.30693 72 ACTTC 158030 1.4378871 10.536239 72 GGGCC 11745 1.4258853 7.1943927 70 CAGAC 39650 1.4020694 9.302003 70 CAACC 2370 1.38428 7.8288283 69 CCCTG 1820 1.3600516 12.148814 71 TCACC 3340 1.3470318 11.948614 72 CGTAT 2335460 1.2864959 9.511429 46 CGGCT 27460 1.2423251 9.881937 53 CCCGA 1140 1.2337668 7.820428 72 CTCTA 133525 1.2149204 9.822865 72 TCCGG 26040 1.1780825 10.657482 72 ATTCC 128840 1.1722926 8.165997 72 CTCGC 1560 1.1657584 5.684898 69 CTTCA 127335 1.1585987 8.649249 72 AGCTC 46870 1.1443979 7.1450377 71 CCGGT 25140 1.1373652 10.2815275 72 CCACT 2750 1.1090829 11.220039 72 GAGCC 16250 1.0647125 5.1375666 70 TTCCA 115760 1.0532799 7.975324 72 GTCCC 1365 1.0200386 8.640775 70 CGACC 930 1.006494 5.08287 71 TCCAC 2485 1.0022078 9.471462 72 ACTCG 40785 0.99582386 7.5596256 71 CCGCT 1330 0.9938837 5.4004846 70 TGCAC 39455 0.96335006 6.4303694 69 ACTGA 1191625 0.9506481 12.053662 34 CCAAC 1540 0.89948994 6.54146 71 CAGGC 13340 0.8740469 5.6104746 72 GCCGG 7050 0.8558953 22.192171 51 CCAGG 12155 0.7964048 5.77572 71 ATCCC 1855 0.748127 8.596483 71 CTCGA 30065 0.73407984 7.357334 72 ACCCT 1800 0.7259453 8.597174 72 GACTC 28125 0.68671197 5.2401156 72 GGCCG 5650 0.68593025 11.314412 50 CTGAT 1062000 0.58500624 8.332965 35 GCACT 23610 0.5764718 7.1544333 72 ACGCT 21875 0.5341093 13.081073 1 GCTCG 8610 0.38952726 5.8930535 43 ACTCA 29515 0.38893074 7.898565 72 AGGCC 5730 0.375434 5.8469915 49 TATGC 536600 0.29558787 8.491481 48 TCCGA 10905 0.26626113 12.962193 8 GCTTG 247085 0.2521941 15.427195 61 CCGAT 9770 0.23854846 12.944729 9 TTCCG 12785 0.21554531 8.980506 7 GCTCT 11950 0.20146786 9.047201 3 CCGTG 3850 0.17417884 5.3559403 52 CTTGA 235060 0.12948357 7.669823 62 TTCTG 274825 0.10453168 5.7800326 57 GTCTT 274265 0.104318686 5.7426353 54 TGCTT 265870 0.10112558 5.7383904 60 >>END_MODULE