FastQCFastQC Report
Fri 4 Apr 2014
filtered_174gm_A_BiGosperm_L006_R2.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename filtered_174gm_A_BiGosperm_L006_R2.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 171582441
Filtered Sequences 0
Sequence length 76
%GC 18

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 1470430 61084.34 341843.34 41
GAGCG 14643965 2415.3853 11359.559 72
CGGGG 247955 1101.6149 18436.523 39
GGAAG 19107165 1094.0151 4627.327 72
GGGGC 221220 982.83655 13668.534 42
GGGCG 198920 883.7621 14249.549 43
GGTGG 362950 843.0632 10537.728 72
GGCGG 150555 668.886 14154.308 13
GCGGG 140940 626.16846 14055.603 14
GTAGG 6945215 598.9214 3745.601 72
AGGGG 383215 591.0155 6633.149 28
GGGGA 359065 553.76996 8866.018 21
TAGGG 6314050 544.4928 3501.7385 72
GAGGG 277330 427.71375 4857.0283 27
GCGTC 12829540 340.85315 1714.8082 72
GGGAG 218980 337.72308 4255.6216 30
TGGGG 145180 337.22534 3153.4482 20
AGGGA 5811895 332.77057 2205.892 72
CGTCG 11985830 318.43756 1655.1895 72
GGGAA 5528620 316.55112 2130.4165 72
GGGGT 128410 298.2718 4113.9976 41
GTGGG 117080 271.95444 2214.944 19
GGGTG 105290 244.56845 2084.8347 43
GGAGG 152775 235.61804 3603.2603 18
GAGTG 2602620 224.43724 1959.0343 72
GATCG 24299310 224.10396 820.4318 72
ATCGG 22761360 209.91998 792.56854 72
TCGGA 21529705 198.56087 783.0041 72
GTCGT 10891575 151.28758 815.4043 72
TCGTG 10015580 139.11972 793.9742 72
CGTGT 9183370 127.56005 742.02893 72
AGCGT 13723025 126.56263 603.624 72
CGGAA 20407110 124.96261 507.97742 72
AGAGC 15978710 97.84537 444.6056 72
CGGTG 367570 91.31137 1337.9324 72
GTGTA 10447480 50.375977 330.6555 72
TCGGG 194335 48.276505 964.1055 38
TGTAG 9477720 45.69996 310.1067 72
AAGAG 20671395 43.940773 223.70648 72
GGTCG 173940 43.21 579.5441 72
GGCGC 89945 42.737133 1399.8507 44
GGGCC 88055 41.839108 827.2551 45
GAAGA 18180495 38.645916 165.46942 72
GCGCG 76245 36.22762 122.804016 72
CCGGG 75685 35.961536 808.8547 39
GCGGC 74020 35.17041 590.88007 11
CGGCG 72355 34.379295 586.017 12
GCCGG 67040 31.853886 552.4763 47
GTGGT 231265 30.036623 443.30316 72
GGCCG 61585 29.261955 331.37814 46
GTCGG 94880 23.569994 272.91867 13
AAGGG 340355 19.487638 213.49615 27
CGCGG 40300 19.148443 70.08032 15
CGCCG 323720 16.45013 209.64014 46
CGGGA 96530 15.921722 332.21796 4
CTCGG 598370 15.897396 209.86096 72
CGGGT 63830 15.856585 370.42877 15
GAGAG 248035 14.201693 98.97464 7
GGCGT 54780 13.608393 139.28078 13
CGTGG 54285 13.485425 118.698456 15
AGAGG 230300 13.186243 175.30621 26
GGGTC 52355 13.005975 330.5506 42
CGGGC 27295 12.969149 35.59984 42
GGAAA 5568205 11.836222 80.17751 72
GCGTG 46870 11.643398 132.7631 14
AGTGT 2338620 11.276431 97.87545 72
AGCGG 66325 10.93969 209.74323 10
GGGTA 125670 10.837166 192.46942 29
GAAGG 182170 10.430472 96.47737 72
GGTAG 118290 10.200751 173.07347 30
AGGGC 61735 10.182612 69.898735 11
GAAAG 4769775 10.139016 74.55352 72
TTGGG 76690 9.960472 95.040085 38
AGAGT 3056185 9.784409 78.81701 72
AGGGT 113215 9.763109 202.34413 28
AGATC 26962930 9.231951 35.73861 72
CCGCG 169780 8.627527 10.951615 48
GTGCG 31270 7.7680616 29.330425 72
GCGCC 149875 7.616036 151.93724 45
GGAGA 132290 7.5745025 151.94344 31
TCGCG 277215 7.3650026 11.772322 71
ACGGG 42910 7.077604 109.3006 3
CGACG 400110 7.0579505 10.984826 71
GTGTG 54145 7.032335 116.64642 72
CGCGC 133860 6.802219 8.707419 47
TGGGA 78415 6.762127 73.12199 4
CGCGA 383240 6.760363 7.461766 14
CCCCC 11325780 6.5828023 7.6132603 26
CGGAG 39365 6.4928894 64.309875 5
TCGGT 466100 6.474283 87.32546 72
CAGGG 38330 6.3221755 32.298798 72
GCGGT 24335 6.045276 23.327967 48
GTAGA 1884245 6.03243 56.46908 72
CGCGT 226520 6.0181465 11.73407 71
GCAGG 36325 5.99147 38.71106 72
GTTGG 44945 5.837442 79.83682 18
CTGGG 23205 5.7645626 121.400696 39
GGTGC 22195 5.513659 156.64548 44
TGTGG 42125 5.4711814 34.99394 40
AGGCG 32660 5.386962 45.027657 12
GGGTT 37510 4.871786 38.346954 16
GTCGC 182715 4.8543425 53.65512 44
ATGGG 56205 4.846844 38.10708 3
ACGCG 269685 4.7572503 5.323781 3
GTGGC 18970 4.712508 51.246502 45
GGTGT 35640 4.628911 19.261332 71
GGACG 27570 4.547414 37.404884 1
TGGGC 18100 4.4963837 106.93232 42
GGTTG 33905 4.40357 39.790337 17
GCCGT 160295 4.258692 83.82834 47
GACGG 25555 4.215059 37.539417 2
GGGCT 14825 3.6828115 24.15788 43
TGGTG 28255 3.6697502 25.44466 39
GGAGC 21990 3.627045 25.363111 44
GCCGC 71000 3.6079304 38.43338 47
GGGCA 21645 3.5701406 16.030079 70
GTGGA 41255 3.5576298 36.54572 16
GCCCG 69655 3.539583 52.90306 47
GAGGC 19880 3.2790205 32.30214 11
TGGCG 13095 3.2530468 21.1982 46
GGCAG 19270 3.1784067 12.4682865 72
TGGAG 36750 3.1691406 38.744102 17
TGCGG 12545 3.1164162 17.448954 40
GCTGG 12530 3.11269 10.750049 14
GATGG 35935 3.098859 18.9744 2
GGGAC 18340 3.0250118 14.199791 42
GGCTG 12005 2.98227 10.031798 13
GCGGA 17700 2.9194498 17.519659 16
AGGAG 50880 2.9132264 16.797491 72
TGGGT 22050 2.8638468 26.292616 42
GTCCG 107365 2.8524559 5.048272 13
GCGAG 15640 2.579672 11.755813 72
GAACG 420670 2.575966 7.2453265 72
GGCCC 49360 2.5082738 58.713146 46
GGATG 28665 2.4719293 19.059546 1
GGGAT 27970 2.4119964 23.3713 33
GAGGA 40985 2.3466704 15.920117 29
GACCG 128185 2.2611868 5.1177864 71
TGGTC 159530 2.2159245 38.145203 72
GCGAC 124380 2.1940663 6.7559857 71
TCTCG 1420725 2.1105423 14.694156 72
GAGCA 338005 2.0697682 9.572741 71
AGTGG 22815 1.9674542 13.037465 72
AGCGC 109480 1.93123 26.288101 72
CGAGG 11495 1.895993 11.70426 40
GAGGT 21975 1.8950168 11.803701 41
CGCCC 343155 1.8649259 7.8270316 46
AGGTG 21165 1.8251663 7.9612236 12
TAGAT 10116565 1.810989 8.029562 72
GTGAG 20400 1.7591964 11.237053 72
GGCGA 10635 1.754144 5.7616444 44
GCCGA 96410 1.7006748 6.0389643 47
GGTGA 19380 1.6712364 30.360949 31
AAGAT 13807315 1.6410997 5.655627 72
AAGCG 265645 1.6266727 7.401827 72
CAGAT 4714305 1.6141505 5.329566 72
TGAGG 17775 1.532829 6.3367167 40
GCGTA 159175 1.4680148 5.192066 71
CCCGG 28275 1.43682 10.8333645 38
GGAGT 16595 1.4310716 8.412937 34
CCGTG 53405 1.4188554 7.555148 72
TCGCC 496535 1.4108391 6.8823757 45
CGGCC 23755 1.2071322 8.068712 46
CCGGA 67770 1.1954645 15.46066 48
CGTGC 41710 1.1081445 6.436221 72
TCCGG 39080 1.0382711 37.973373 38
TTCGG 74625 1.0365659 19.257248 37
ACCGG 55905 0.9861656 5.812959 3
CGTAG 91425 0.8431805 5.195527 72
AGAGA 392155 0.8335961 5.2366776 71
CGTGA 81105 0.74800277 10.142067 72
GCCCC 133735 0.72680235 5.4837427 47
GATCT 1332150 0.6869681 6.387825 72
AAAGG 302740 0.64352834 8.011515 26
CCGGT 20900 0.5552678 7.9623227 49
AACGG 88625 0.54269373 6.0465646 2
GTGCC 17490 0.4646715 16.308176 45
TGCCG 14240 0.378326 11.526808 46
CGGAT 34255 0.31592178 7.9369807 49
TCTGG 15845 0.22009228 5.2122135 38
GGATC 23505 0.21677832 5.544605 50
Produced by FastQC (version 0.10.1)