FastQCFastQC Report
Fri 6 Jun 2014
filtered_Y38_control_GTGAAA_L005_R1_tr.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename filtered_Y38_control_GTGAAA_L005_R1_tr.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 3262139
Filtered Sequences 0
Sequence length 19-36
%GC 17

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CCCCC 50 509094.34 1651751.6 32
CTCCC 130 10524.399 324060.84 24
ACCCC 35 4303.0 39323.645 22
CCTCC 50 4047.846 129624.34 24
CACAC 2260 3354.955 47606.094 12
CACCC 20 2458.857 19673.611 23
CCCGC 10 2286.0564 36663.81 26
CCCCG 10 2286.0564 36487.062 17
ACTCC 1930 1886.628 17978.246 22
CCCCA 15 1844.143 39347.227 23
CTCCA 1800 1759.5496 18482.77 25
CCCAC 10 1229.4286 39230.285 12
CGCCC 5 1143.0282 36474.59 10
CCACG 245 676.2823 10129.265 12
CGCGC 110 564.59656 830.2734 31
CGCTC 300 545.298 14511.815 2
GCCAC 180 496.86044 14533.806 11
CCCTC 5 404.78458 12939.982 21
CCCCT 5 404.78458 13133.197 32
CCCAG 135 372.6454 11067.578 26
CCGTC 180 327.17877 4640.926 16
GCACC 115 317.43863 8808.367 11
CGTCC 145 263.56067 7541.5044 16
CTTCC 325 209.20062 5340.3677 6
CACGC 60 165.62015 3082.8198 14
CACCG 60 165.62015 2642.417 14
CGGCG 1360 156.72673 260.34677 30
CACCA 95 141.02689 3315.8474 12
TCGCG 3275 133.65417 208.36497 10
TCCCA 135 131.96622 3915.8408 25
CGCGG 1145 131.95009 240.05104 25
CGTCG 3150 128.55286 214.9103 16
CGCGA 2015 124.88078 208.3879 24
CGACG 1870 115.89432 188.68187 25
GCGCG 990 114.08784 239.0208 5
ACCAC 75 111.337006 3079.001 13
CGCAC 35 96.611755 895.5884 32
CCAGC 35 96.611755 2656.2185 26
CGCGT 2335 95.292366 163.82928 27
CACGT 4220 92.61899 654.8131 14
CCACA 60 89.06962 2368.4624 12
ACGCG 1425 88.315186 158.36465 19
AGCAC 2620 87.32493 1286.9326 10
ACACC 55 81.64714 1657.9237 13
ACACG 2335 77.825836 1036.9546 13
GCACA 2330 77.659195 1175.2567 11
GCCGC 15 76.99044 828.2578 30
ACGTC 3075 67.48896 640.80945 15
CTCCG 35 63.618095 1455.1749 24
ACCTC 65 63.539284 1564.2631 23
CGGAC 925 57.327408 108.768074 4
CCGCA 20 55.206722 893.03314 31
GCCCA 20 55.206722 1321.2552 11
TCCAC 55 53.76401 1419.6833 30
CCGCG 10 51.326965 824.18713 27
GGCCC 10 51.326958 820.85425 22
AACCC 30 44.53481 712.2297 22
CCAAC 30 44.5348 474.55658 21
GTCAC 1930 42.358925 580.83325 30
TCACG 1910 41.91997 585.797 31
CAGTC 1890 41.48102 529.3878 28
ACCCG 15 41.405037 442.30103 25
CCAGT 1860 40.822586 525.1147 27
TCCAG 1815 39.83495 449.81116 24
GCGGC 345 39.757885 92.796425 29
CGGGC 335 38.605484 110.32119 10
TCCGC 20 36.353195 582.06995 24
TCCCG 20 36.353195 291.2518 25
CCCGT 20 36.353195 291.51657 26
CCGCT 20 36.353195 291.2518 25
GCTCC 20 36.353195 291.03494 24
CCATC 35 34.213463 468.73773 21
GCGTC 820 33.464558 65.111755 6
CGTCT 2250 32.517773 332.103 16
TCGGC 795 32.444298 65.1644 2
GGCGC 270 31.114868 73.86186 25
CGGTC 735 29.99567 52.425365 27
CGTTC 2030 29.338259 55.339813 12
CACTC 30 29.325825 467.88422 14
CCCGA 10 27.603361 440.56897 17
CGACC 10 27.60336 442.30103 25
GACCC 10 27.60336 441.97174 24
ACGCC 10 27.60336 447.79413 32
CTGCC 15 27.264896 580.0037 14
TTCGC 1870 27.025883 50.729954 9
CGAGC 435 26.959373 79.38585 24
CGATC 1210 26.556633 185.59532 10
GTCGC 650 26.526781 71.79337 22
TCTCC 40 25.74777 205.5648 2
CGCCG 5 25.663483 818.9343 9
CCCGG 5 25.663483 820.0556 20
CCGGC 5 25.663479 818.90906 15
GCGCC 5 25.663479 818.9342 9
TCACC 25 24.438187 311.92282 12
TCGTC 1690 24.424463 43.812233 10
AACTC 2045 24.137781 211.54504 23
CAACC 15 22.2674 238.37381 27
ATCGC 1000 21.94763 42.02158 7
TCGAC 990 21.728155 45.683945 24
ACGGC 345 21.381573 49.905407 29
ACGAC 630 20.997978 37.740757 31
GCGAC 330 20.45194 49.44178 8
CTCTC 30 19.310827 308.403 19
TTCCC 30 19.310827 205.39876 5
CGTAC 875 19.204176 31.54055 1
AGCGC 305 18.90255 49.441772 10
AACGC 560 18.664871 53.21011 18
CTCGC 10 18.176598 290.8663 23
TCGCC 10 18.176598 290.0121 8
CCTCG 10 18.176598 290.69202 22
CGTGC 445 18.160643 45.595417 17
TACGC 805 17.667843 42.20121 25
TGCGC 425 17.344433 45.623325 19
CCCAA 10 14.844936 237.8671 25
ACCCA 10 14.844936 475.1045 23
CCCAT 15 14.662913 158.57893 32
CCTCA 15 14.662913 158.12648 31
CTCAC 15 14.662912 158.57892 32
TACCC 15 14.662912 156.33257 22
CCTAC 15 14.662912 311.9338 8
CGAAC 420 13.998652 53.229782 19
CGCCA 5 13.801681 441.97177 24
CAGCC 5 13.80168 440.6757 18
AGCCC 5 13.80168 440.41837 10
ACCGC 5 13.80168 440.75882 2
GACGC 220 13.634625 40.101086 31
CCTTC 20 12.873885 206.13048 24
GAGCC 175 10.845726 326.31573 9
ATCGG 21735 10.710405 37.846912 2
CGGGG 4120 10.660069 14.562164 28
GATCG 21270 10.481266 37.891438 32
CGGAA 13190 9.870528 48.354717 4
CCCTA 10 9.775275 311.93384 7
CCACT 10 9.775274 158.57892 32
CACCT 10 9.775274 155.96141 12
ATCCC 10 9.775274 155.96404 3
ACCCT 10 9.775274 156.18045 20
CCCTT 15 9.655415 102.9438 22
TCCCT 15 9.655415 205.39877 6
CTCCT 15 9.655415 205.39972 15
TCGGA 18475 9.103969 34.986607 3
CGCCT 5 9.088299 290.00186 13
GCCTC 5 9.088299 290.05786 16
GCCCT 5 9.088299 290.00186 12
TGCCC 5 9.088299 290.01205 11
TCCGA 410 8.9985285 185.59532 8
CCGAT 390 8.559576 182.09352 9
ACGCT 375 8.23036 175.22527 1
GGCGG 3145 8.137359 11.988325 20
GCGGG 2765 7.1541486 10.339096 21
GGGCG 2760 7.141211 11.146282 11
TCGGG 7465 6.840057 9.356499 10
TTCCG 465 6.72034 122.213066 7
GACGG 4565 6.3521385 7.674933 32
GGCAC 100 6.1975574 69.21849 9
AGCCA 175 5.832772 170.17291 10
CGAGG 4155 5.7816286 8.214561 10
CCGGG 50 5.7620125 36.772434 13
GGGGG 98380 5.715158 9.535725 2
TGCCG 140 5.7134604 65.11175 14
CGGAG 4030 5.607694 8.328304 31
GTCGG 6025 5.520608 6.870936 4
GACAC 165 5.4994707 101.04017 9
GGTCG 5900 5.4060726 7.604754 17
TTCGG 16480 5.3475413 7.2737365 24
GCTCT 365 5.275105 117.599075 3
GCACG 85 5.2679234 59.88649 29
GCGAG 3780 5.2598224 8.658593 7
GGACG 3715 5.1693754 7.3262773 15
CGGTA 10385 5.1174397 6.9185824 6
CGGGA 3675 5.113716 7.7705326 10
GGCGA 3625 5.044142 7.770259 6
TCGGT 15420 5.0035853 6.2912703 25
GCCGT 120 4.8972526 65.11206 15
CTACC 5 4.887637 155.96141 12
CATCC 5 4.887637 155.96141 13
TTTCG 42410 4.8734016 5.3765397 25
CGGGT 5300 4.8563027 6.7301826 1
AGCGG 3460 4.814545 6.438213 4
ACGGA 6410 4.796822 6.8832 30
TCGAG 9480 4.6714816 5.645789 30
CAACG 140 4.6662173 53.177155 14
CGAGA 6045 4.52368 6.2232814 22
GTTCG 13640 4.4259987 5.28804 20
AGATC 16630 4.40712 22.484375 32
ACGGG 3165 4.4040565 6.0414057 28
GAGCG 3150 4.3831854 7.1348567 25
GCGGA 3140 4.3692703 6.6668124 19
GGCGT 4760 4.36151 6.3356824 28
AGGCG 3120 4.3414407 7.9924006 3
TATCG 24575 4.2885146 5.1549473 1
GCGGT 4680 4.288207 6.0047297 21
TGGCG 4500 4.1232758 5.8477035 3
CGGTT 12655 4.1063795 4.8918757 26
TACGG 8330 4.104793 6.0537596 4
CGGAT 8220 4.0505886 5.3463664 9
TCGTT 35205 4.045464 4.5659947 16
TTCGT 34870 4.006968 4.5284657 15
GAACC 120 3.999615 74.58384 21
GTCGA 7870 3.8781173 5.0335937 17
CGAAA 9595 3.8615081 4.8174734 31
GACGA 5155 3.8576627 4.8007183 26
AGCGA 5140 3.8464377 5.0264964 22
TTCGA 21880 3.8182173 4.546536 21
ACGGT 7745 3.8165212 4.717215 5
TAGCG 7695 3.791883 5.1889377 5
GTCGT 11680 3.7900045 4.8148565 7
CGGTG 4135 3.7888322 4.991888 25
AGACG 5060 3.7865713 5.0146475 3
GCGAT 7565 3.7278228 4.717382 7
CACGG 60 3.7185342 49.44003 14
ACCGG 60 3.7185342 29.687998 2
CGTTT 32195 3.6995797 4.418598 8
CGAAG 4930 3.689288 4.8953414 7
GTACG 7455 3.6736171 4.7173815 11
GACGT 7400 3.6465147 5.0316963 4
CGAGT 7360 3.6268039 4.5599957 15
CGTCA 165 3.6213586 17.621298 27
AACGG 4775 3.573296 4.6592646 18
CGTTA 20105 3.508467 4.151822 1
AGAGC 4670 3.494721 36.65536 8
AGTCG 7055 3.4765084 4.799712 2
GCGAA 4620 3.4573042 4.67026 23
GCCGG 30 3.4572074 36.772434 12
ACGAG 4610 3.4498208 4.7758546 3
CGATA 12955 3.4332078 4.016888 8
ATCGT 19655 3.4299388 3.953574 14
AAGCG 4580 3.4273708 4.7844863 21
ATTCG 19580 3.416851 4.1329093 23
AAACG 8490 3.4168012 4.3036685 17
ATCGA 12875 3.4120069 4.524146 15
GCGCA 55 3.4086566 19.846466 24
ACACA 190 3.4057043 48.61897 10
TCGAT 19510 3.4046357 4.3163548 16
TCGTA 19490 3.4011455 4.1073647 24
TGACG 6830 3.3656344 4.3276095 1
CGTAG 6825 3.3631706 4.4217105 25
GCGTT 10330 3.3519478 4.2453575 9
TGTCG 10180 3.3032746 4.0906854 16
CCTGA 150 3.2921445 84.12336 19
ACGAA 8140 3.2759433 4.302197 10
CGTAA 12350 3.2728765 4.1469407 1
CGTGG 3570 3.271132 4.8244443 7
TTACG 18715 3.2659016 4.1206956 3
TCGAA 12240 3.2437258 3.8838384 29
CCTCT 5 3.2184715 102.703 7
TCCTC 5 3.2184713 102.69938 14
CGATT 18325 3.1978443 3.7469175 25
TACGA 11865 3.1443465 3.7318666 23
AGCGT 6370 3.1389592 4.009774 8
GCGTA 6330 3.1192484 4.2789288 28
ACGTT 17840 3.1132083 3.730969 7
TGCGG 3395 3.1107824 4.5320535 9
CGGCA 50 3.0987787 19.779833 16
ATACG 11665 3.0913448 3.9776783 2
AATCG 11625 3.080744 3.974605 9
ATGCC 140 3.0726686 35.01675 13
ACCGT 140 3.0726678 24.511835 15
CGCTG 75 3.0607827 52.09947 16
CGTTG 9420 3.056665 3.9871235 16
CGATG 6160 3.0354772 4.4830446 17
GCGTG 3305 3.028317 4.678084 13
AACGA 7485 3.0123386 3.7107565 31
TCGTG 9280 3.011237 3.786165 21
GGGCC 25 2.881006 36.83951 21
TCTTC 555 2.8405228 42.461647 5
ACGTG 5745 2.8309767 14.427811 31
CGTGA 5740 2.8285124 15.10861 32
TCTCG 185 2.6736836 25.364977 7
CACGA 80 2.66641 10.719192 28
GCAAC 80 2.66641 85.08646 11
TCGCA 120 2.6337156 10.5503025 25
GGGGC 1015 2.6262062 5.3665395 4
CTCGT 180 2.6014218 25.364977 8
AACAC 145 2.5990903 25.73855 13
CGCAT 115 2.5239775 14.079859 26
GACTC 115 2.5239775 45.604813 21
GAGCA 3370 2.5218863 32.237614 9
CGCAG 40 2.479023 29.665068 9
TACAC 210 2.478696 9.425225 19
CTCGG 60 2.4486263 13.022351 6
CGGCT 60 2.4486263 19.537298 16
GGCCT 60 2.4486263 13.02281 11
GTCCG 60 2.448626 19.540892 17
GCTCG 60 2.448626 6.6204524 32
CGCAA 70 2.333109 10.635432 4
TCCTG 160 2.312375 55.374104 18
GTCCT 160 2.3123748 55.360683 17
TGGCC 55 2.244574 13.203128 31
ACAAC 125 2.2405949 31.458221 13
TCCGT 155 2.2401133 18.526114 25
CTCAG 100 2.194763 42.169796 24
CCAGG 35 2.169145 49.69826 26
GGACC 35 2.169145 19.776014 14
CCGGA 35 2.169145 19.792 1
CCGAG 35 2.169145 9.939653 26
CAGCG 35 2.169145 10.025271 31
CCGTT 150 2.1678514 6.9230695 1
CACAA 120 2.1509712 34.31806 12
CTCTT 420 2.1495848 41.645077 4
CATCG 95 2.0850248 14.138543 29
ACGCA 60 1.9998075 10.690983 26
CCAAG 60 1.9998074 37.418438 26
CCGTA 90 1.9752867 7.0399294 26
GCCTT 135 1.9510664 20.769207 1
CAACA 105 1.8820997 22.878706 12
TCTGC 130 1.8788047 23.113132 22
TCGCT 130 1.8788047 6.9536076 26
CTGCT 130 1.8788047 23.12699 23
CTGAC 85 1.8655487 38.556545 19
TACCG 85 1.8655485 14.018023 1
GCAGC 30 1.8592671 9.9658785 28
CAGGC 30 1.8592671 19.776346 3
GACCG 30 1.8592671 19.846462 24
GCGCT 45 1.8364697 13.040639 20
CTTCG 125 1.806543 9.223303 13
GCATC 80 1.7558104 10.587756 28
GTACC 80 1.7558104 7.083332 30
CGCTA 80 1.7558104 14.097038 27
GTCCA 80 1.7558103 31.811722 29
GGCCG 15 1.7286036 18.40441 19
CAGCA 50 1.6665063 32.112083 27
CACAG 50 1.6665062 15.966043 1
CGCTT 115 1.6620195 6.930096 21
CTACG 75 1.6460723 10.505201 3
CGACT 75 1.646072 14.138543 29
GCACT 75 1.646072 10.559893 26
CCGGT 40 1.6324174 19.533857 3
GCTGC 40 1.6324173 6.6204524 32
CATAC 135 1.5934474 9.415907 12
ATCTC 205 1.5933403 13.640665 6
AGGCC 25 1.5493894 29.665068 10
GCTAC 70 1.536334 10.513516 2
AGTCC 70 1.536334 31.76327 28
TGCAC 70 1.536334 7.083332 30
CATCA 130 1.5344311 13.286088 28
CACAT 130 1.5344309 15.065452 12
AACCA 85 1.5236046 5.7400537 24
ACCAA 85 1.5236045 8.595166 21
CTTCA 195 1.5156164 7.4724317 25
AGACC 45 1.4998556 10.667134 23
GTGCC 35 1.4283652 19.54563 18
ATCCA 115 1.3573813 20.776745 23
CCGAA 40 1.333205 15.968935 19
AACCG 40 1.333205 10.650369 20
GACCT 60 1.3168577 10.505072 15
ACTCA 110 1.2983648 20.776745 23
ACACT 110 1.2983646 5.7443614 32
AATCC 110 1.2983646 22.651958 22
CAAAC 70 1.2547331 8.588006 19
ACTTC 160 1.2435826 7.462541 23
GGCCA 20 1.2395115 10.053958 32
GCCGA 20 1.2395115 9.981082 29
AGCCG 20 1.2395114 10.053957 32
CACTA 105 1.2393482 7.618755 30
AACCT 105 1.2393482 18.876635 22
CTGTC 85 1.2284493 6.9300966 21
CCGTG 30 1.2243131 6.526672 22
TGAAC 4580 1.2137469 5.250274 20
CCTTA 155 1.2047207 11.169566 2
ATCAC 100 1.1803315 7.592049 28
GACCA 35 1.1665545 10.650369 20
ACCAG 35 1.1665543 5.345491 26
TGCCT 80 1.1561874 6.9243217 19
TCATC 145 1.1269968 6.2402925 27
TCCAA 95 1.121315 13.239088 25
ACTAC 95 1.1213149 5.7279716 31
TGACC 50 1.0973815 7.024225 23
CTCTG 75 1.0839257 6.918813 16
TGTCC 75 1.0839257 6.917476 14
CTACA 90 1.0622984 5.649744 9
ATTCC 135 1.0492729 7.4580703 22
TCCGG 25 1.0202609 6.585538 30
GCCTG 25 1.0202609 6.620454 32
ACTCT 130 1.0104109 6.215058 22
ACCAT 85 1.0032817 5.657479 20
CACTG 45 0.98764336 7.024225 23
ACTCG 45 0.98764336 7.003597 11
AAACC 55 0.98586184 5.719678 5
TACCA 80 0.94426525 5.669671 24
GTCTG 2785 0.9036955 7.6649723 17
GCCTA 40 0.8779052 7.0131855 20
CCATG 40 0.8779052 7.003597 9
CGACA 25 0.83325315 5.3454914 26
CAAGC 25 0.83325315 5.352014 27
CAGAC 25 0.83325315 5.378448 30
AAGCC 25 0.83325315 5.359596 28
TCACA 70 0.8262321 7.543306 20
TAACC 70 0.8262321 5.655136 19
GGCTC 20 0.8162087 6.572465 29
CTGCG 20 0.8162087 6.572465 29
CCAAA 45 0.80661416 5.749552 26
CTTCT 155 0.7932992 8.994887 20
AGTCA 2970 0.7870804 6.8287263 29
TTCCA 100 0.77723914 6.2270265 25
CATGC 35 0.768167 10.572779 27
ACTGC 35 0.768167 7.048519 27
TCAGC 35 0.768167 7.083332 30
TGCCA 35 0.76816696 7.004702 16
CTGAA 2855 0.7566043 5.6713343 19
CTAAC 60 0.70819885 5.655135 19
CCAGA 20 0.6666025 5.3251843 20
ACAGC 20 0.6666025 5.406964 32
GCCAT 30 0.6584289 7.0059915 17
CCTAA 55 0.6491824 5.6495447 5
GCCAG 10 0.61975574 9.930626 25
CCTGG 15 0.61215657 6.620454 32
GCTCA 25 0.5486907 7.020016 22
CCATA 45 0.5311492 5.6530457 18
GCCAA 15 0.49995187 5.333567 23
ACCGA 15 0.49995187 5.3454914 26
GGTCC 10 0.40810436 6.511405 8
AGCCT 15 0.32921442 7.0034676 3
CTGGC 5 0.20405218 6.5114055 7
Produced by FastQC (version 0.10.1)