FastQCFastQC Report
Fri 6 Jun 2014
filtered_Y38_control_GTGAAA_L005_R2_tr.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename filtered_Y38_control_GTGAAA_L005_R2_tr.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 3262139
Filtered Sequences 0
Sequence length 19-36
%GC 17

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 60 331473.72 4440570.5 27
GAGGG 235 11672.065 199613.89 27
GGGCG 60 8473.352 90004.91 9
AGGGG 145 7201.9126 71521.625 20
GGGGT 60 4517.605 60601.09 28
GGGGA 90 4470.1533 87426.65 21
GGAGG 75 3725.1272 31745.621 18
GGGGC 25 3530.5627 45001.27 10
GAGCG 2675 3396.3286 51787.88 9
CGGGG 20 2824.4504 44951.156 14
GCGGG 20 2824.4504 22581.344 19
GGAAG 5350 2388.997 20534.46 5
GGGAG 40 1986.7347 15832.89 11
GTGGG 25 1882.3356 23970.838 13
TGGGG 25 1882.3356 24093.62 20
GGTGG 20 1505.8685 12221.663 31
GGCGG 10 1412.2252 22565.656 18
TAGGG 2025 1370.7683 33770.65 19
GTAGG 1760 1191.3837 27798.166 18
GGGTG 15 1129.4014 12190.684 30
TCGGG 530 1020.0912 24505.299 14
CGGGT 505 971.9735 24504.732 15
GGGAA 2090 933.27185 22722.719 21
AGGGA 1990 888.6176 22076.76 20
GTCGG 455 875.73846 22059.299 13
GAGTG 1205 815.6917 21219.957 27
GGGTA 790 534.7688 8947.381 16
GCGCG 135 487.35315 1731.7177 19
GGTAG 605 409.53818 8756.623 17
CCGGG 75 270.75177 3463.4353 19
CGGGC 70 252.70164 4620.777 20
GGGCC 65 234.65152 6354.3916 21
GATCG 12865 222.6152 1159.8907 32
GGGTC 115 221.34052 4003.8489 21
AGGGT 320 216.61522 2707.6592 20
CGCGG 60 216.60141 1729.5928 17
CGGCG 60 216.60141 2310.6877 21
TTGGG 195 200.10172 4594.307 19
TGGGT 190 194.9709 5089.7075 20
ATGGG 285 192.92294 4329.5703 19
GTTGG 185 189.84009 5246.9893 18
AAGGG 410 183.08203 3284.472 19
GCCGG 50 180.50117 1730.5145 18
ATCGG 10410 180.13405 952.8688 32
CGTCG 3585 176.38365 2093.592 12
TGGGA 255 172.61525 3249.1912 20
CAGGG 135 171.4035 4060.334 19
AGGGC 130 165.0552 3250.2808 20
GATGG 240 162.46141 3569.4143 18
GGGTT 155 159.0552 3776.724 21
CGGGA 125 158.70694 3453.4236 20
GGGCA 120 152.35867 3047.5334 21
TCGGA 8665 149.93867 909.1897 3
GGGAT 220 148.92297 3357.9329 21
GCGTC 2615 128.6592 2187.8809 11
GGCCG 35 126.3508 1155.918 22
GGCGT 65 125.10551 924.4243 22
ACGGG 95 120.61728 2639.217 19
TGGGC 60 115.482 3079.4849 20
GACGG 90 114.26899 3043.1345 18
CGCCG 1230 113.50662 250.02107 12
GAAGG 245 109.40267 2568.6704 18
GGGAC 85 107.92072 2031.689 21
CCGCG 1155 106.58549 193.1136 28
GGTCG 55 105.85852 1226.9722 12
GGCGA 80 101.57245 609.8096 22
CTGGG 50 96.235016 2154.304 19
AGAGG 210 93.77372 1863.7545 26
GCGGC 25 90.25059 1153.6764 18
CGCGA 2735 88.766556 142.24239 31
TCGCG 1795 88.31483 158.39905 28
CGGAA 7310 83.442116 619.6681 4
GCAGG 65 82.52761 2231.6323 18
CGACG 2490 80.81488 145.942 25
CGCGC 860 79.36235 134.05975 29
CGTGG 40 76.988014 1225.2367 15
GGGCT 40 76.98801 2155.9187 21
GCGGA 60 76.17934 412.18216 31
CGGAG 55 69.831055 611.69855 25
AGCGG 55 69.83105 606.8733 10
GGACG 50 63.482777 609.8096 22
GGAGC 50 63.482773 409.87903 29
CGCGT 1280 62.976585 133.11131 15
ACGCG 1870 60.6923 93.29903 17
GTCGT 2205 57.840332 810.06537 13
GCTGG 30 57.74101 922.6317 18
GGCTG 30 57.740997 1225.0297 5
GGCGC 15 54.150352 577.95905 22
CGAGG 40 50.786224 404.58224 10
GCGTG 25 48.117508 308.49997 23
AGCGT 2680 46.37456 769.2015 10
GGTTG 45 46.17732 491.6432 17
GTGTG 45 46.17732 490.5007 9
GGCAG 35 44.437946 808.1083 5
AGGCG 35 44.437946 405.5352 17
TCGTG 1660 43.5442 722.22943 14
CGTGT 1595 41.839157 734.73676 15
AGAGC 3410 38.92443 538.38776 8
GCGGT 20 38.494003 308.14145 22
GTGCG 20 38.494 309.4097 27
GAGGA 85 37.956028 287.13934 27
GAGAG 85 37.956028 498.03757 9
GGATG 55 37.23074 538.55817 5
GGTGT 35 35.915695 329.92642 27
GTGGT 35 35.91569 327.00046 9
TGTGG 35 35.91569 333.13434 31
GGAGA 80 35.723324 285.4078 18
GTCCG 715 35.17832 71.69397 30
TGGAG 50 33.846127 540.5323 17
AGGAG 75 33.490616 285.25577 17
TGGTG 30 30.784878 165.63647 29
GGTGC 15 28.870504 311.62634 30
TGGCG 15 28.870499 307.98837 21
GTGTA 3065 28.276695 366.3305 29
GCCCG 295 27.223133 73.77885 19
GTGGA 40 27.076902 323.3993 16
TGTAG 2875 26.523813 343.5556 30
GCCGC 280 25.838905 88.13806 13
GCGCC 280 25.838905 147.08432 11
GCGAG 20 25.393112 203.1689 21
AAGAG 6095 24.469076 195.48445 7
GCCGA 730 23.692719 52.02164 23
GCGAA 1795 20.48955 38.206528 11
GACCG 630 20.447136 36.671593 29
GTGAG 30 20.307676 431.4209 9
AGGTG 30 20.307676 108.66571 26
GAAGA 4950 19.872341 189.05342 6
CGGTG 10 19.247004 308.49997 23
GTGGC 10 19.247002 307.9485 20
GAACG 1680 19.176846 47.28718 9
GACGC 590 19.148905 36.15737 14
GCGAC 580 18.82435 62.0229 16
GCCGT 360 17.712164 78.31867 13
GCTCG 350 17.220161 55.591328 29
GACGA 1485 16.950962 29.318474 26
GAGGT 25 16.923063 108.709946 25
TGAGG 25 16.923063 108.709946 25
GCGAT 945 16.352226 53.068707 29
AGTGT 1680 15.499132 354.9355 28
GTCGC 310 15.252142 46.991203 13
GCGTA 865 14.967912 41.896347 29
GCGCT 300 14.760137 47.649704 29
GTACG 840 14.535311 30.398373 17
GTCGA 820 14.189232 27.5449 13
GCGCA 430 13.955984 52.388 29
GGAGT 20 13.538451 110.93988 32
GGAAA 3250 13.047497 214.03023 22
GCGTT 485 12.722251 46.172794 21
CCCGG 135 12.458044 73.7276 18
GACGT 645 11.161042 30.29939 13
GCACG 340 11.034964 36.302288 18
AGAGT 1775 10.802378 201.2527 26
GTAGA 1710 10.406797 248.94005 31
GTTCG 390 10.230264 25.148813 18
GGTGA 15 10.153839 108.32041 21
AGTGG 15 10.153838 108.239265 19
CGGCC 110 10.150998 73.8246 20
GGCCC 105 9.689589 88.64501 22
TGCGG 5 9.6235 307.38004 17
CCGGC 100 9.228181 59.02308 19
CCGGA 275 8.925339 46.50825 3
GAAAG 2220 8.912444 162.80075 23
CCCCG 3650 8.610235 15.15348 25
ACCGG 250 8.113944 41.48833 18
CTCGG 160 7.872074 70.75476 18
CCGTG 155 7.6260715 86.132835 14
GGTTT 545 7.6220503 71.64936 22
CGGTT 290 7.607119 50.11183 4
TGTGT 525 7.342342 67.53828 28
CCGCC 3040 7.171264 13.1550455 9
CGGAC 220 7.140271 56.835743 4
AGCGC 220 7.1402693 62.05318 10
TCGGT 255 6.6890187 41.7655 3
TCCGG 135 6.6420627 47.16984 18
TTCGG 245 6.426704 46.10615 18
CCCCC 105285 6.3488445 7.235964 15
GAGGC 5 6.348277 202.36537 11
CCCGC 2670 6.2984457 10.1772375 18
TTAGG 675 6.22733 114.983826 18
GTGTT 440 6.1535816 62.360863 16
TTTGG 430 6.0137277 49.163406 18
TCGGC 120 5.9040546 47.00195 3
ACGGT 335 5.796821 38.571686 3
AACGG 505 5.764469 34.527065 2
AAGCG 505 5.764469 67.293304 9
CCGGT 115 5.6580524 39.335526 19
CGGTA 325 5.6237817 38.566475 4
CGCCC 2350 5.543576 7.915629 18
TACGG 320 5.537262 41.32135 2
TTGTG 395 5.524238 44.965164 27
CCGAG 170 5.5174823 36.32753 19
TTGCG 210 5.5086036 37.749443 19
TGGTT 390 5.454311 42.515167 20
CCCGA 6455 5.3554296 8.15827 32
TTGGT 380 5.314457 49.197586 19
ACGGA 465 5.3078775 32.87422 20
TTGGC 200 5.2462893 41.943825 19
GGTAT 565 5.212507 53.17258 22
GGACC 160 5.192924 67.136536 5
GGCCT 105 5.166048 55.138405 22
GGTTC 195 5.115132 41.97527 21
GAGCT 295 5.1046624 88.22613 9
TCGAG 290 5.0181437 27.817366 27
CGTGA 285 4.931624 38.996582 28
CGCTG 100 4.9200463 47.047012 12
ACGGC 150 4.8683667 51.896473 19
GGCAC 150 4.8683662 51.935375 21
CAGCG 150 4.8683662 31.30503 27
CGGAT 280 4.845104 33.227497 21
GGATT 515 4.751222 42.833458 22
GGTCT 180 4.72166 37.77774 21
CGGCT 95 4.674043 31.3304 4
GTGTC 175 4.5905027 46.5752 29
GGTCA 265 4.585545 47.095676 22
CCGTC 3585 4.5088353 6.249265 23
CCTCG 3555 4.471104 6.312595 30
TGGTC 170 4.459346 37.583874 4
GGACA 390 4.451768 45.68724 22
CGGTC 90 4.428041 23.619009 21
GGTCC 90 4.428041 55.13841 22
TGTTG 315 4.405405 62.53828 17
CGAGA 385 4.3946943 20.212223 28
ATTGG 470 4.336068 35.249065 2
CCGAC 5220 4.330805 6.0736923 15
CTGTG 165 4.3281884 42.16898 27
GGAAC 375 4.2805457 43.859753 22
AAAGG 1055 4.2354183 31.316853 2
ACCCG 5105 4.235394 7.4068575 31
TTGGA 455 4.1976824 42.598236 3
CTGGT 160 4.1970315 33.575863 20
GGCTC 85 4.182039 47.238014 21
TGGCC 85 4.182039 55.103878 20
CGTGC 85 4.182039 47.51972 28
GTGCC 85 4.182039 39.175804 16
ACAGG 365 4.1663985 34.654972 18
CGAGT 240 4.1529465 30.640173 28
AGTGC 240 4.152946 36.21111 28
GAGTT 450 4.151553 28.293816 29
CGACC 4990 4.1399837 5.419669 10
CTCGC 3265 4.106373 6.238094 20
CTCCG 3265 4.106373 5.8711967 28
TAGTG 445 4.105426 35.594288 27
CGTCC 3260 4.1000843 6.874245 27
CCGAT 9230 4.082751 5.4638124 24
CTGAG 235 4.0664268 33.380836 27
TGCGA 235 4.0664263 27.685987 20
GGATC 235 4.0664263 38.78467 22
CGAGC 125 4.056972 36.350056 20
CGGCA 125 4.056972 51.92865 20
GGCCA 125 4.056972 51.96118 22
CAGGC 125 4.056972 41.517178 19
TACCG 9105 4.0274587 5.209305 15
TCCCG 3180 3.9994688 6.829151 17
TGGAC 230 3.9799066 44.29758 20
CCGAA 13555 3.9552467 5.0894613 21
TGCGC 80 3.9360366 39.59976 28
TGCGT 150 3.9347165 29.222483 15
ACCGA 13425 3.9173129 5.253975 8
CACGG 120 3.8946931 31.13788 19
ACGTG 225 3.8933873 36.162575 27
GGTTA 415 3.8286555 31.017338 22
TAGGT 415 3.8286555 35.404236 19
CGAAG 335 3.8239548 29.16759 17
GAGCA 335 3.8239543 25.572147 21
CTTGG 145 3.8035595 62.63998 2
TGTCG 145 3.8035595 33.44443 12
ACGAG 330 3.7668808 25.690163 27
CCGCT 2985 3.7542183 6.6992927 29
GGAAT 615 3.7427955 36.050373 22
TAGCG 215 3.720348 30.293163 14
TCAGG 215 3.720348 33.05708 2
TGGCA 215 3.7203474 33.056973 4
AGGTC 215 3.7203474 46.830715 4
AAGGA 925 3.7135186 30.812782 19
AATGG 610 3.7123668 36.952908 18
TGGAA 610 3.7123663 32.940998 4
AGCGA 325 3.7098064 25.461664 10
TCGCC 2935 3.6913335 5.7161875 31
CCTGG 75 3.6900346 55.031483 18
CGAAA 35915 3.6857493 4.440201 32
GGTAA 605 3.6819375 32.153038 22
AGGAA 915 3.673372 31.474215 20
TCCGC 2920 3.6724682 6.276107 28
GTGCT 140 3.6724021 50.8093 29
ACCGC 4425 3.6712277 5.148842 7
ATGGA 600 3.6515083 33.914433 3
ATGCG 210 3.6338282 25.035627 27
ATAGG 595 3.621079 33.06313 18
CTCGA 8150 3.605029 4.7928696 8
TCCGT 5375 3.6041825 4.8094025 11
AGGTT 390 3.598013 32.474003 20
CAAGG 315 3.595659 36.478916 18
AAGGT 590 3.5906498 36.821384 3
AAGTG 585 3.5602205 49.895576 27
CCCGT 2830 3.5592756 6.478562 28
TATGG 385 3.5518854 38.18649 2
GGTAC 205 3.5473084 30.473673 22
ACTGG 205 3.5473084 27.547567 2
CGATG 205 3.5473084 25.214981 30
GTGCA 205 3.547308 41.89634 29
TCTGG 135 3.5412452 37.58399 2
CGTTG 135 3.5412452 46.081596 17
CAGGA 310 3.538585 36.34908 3
TCCGA 7975 3.5276206 4.5322948 22
TCTCG 5260 3.5270693 4.7381763 25
TTCCG 5230 3.5069528 4.6161904 22
ATGTG 380 3.5057569 33.77014 14
GGATA 575 3.4993618 33.12737 22
GGCAA 305 3.4815106 32.878475 21
GAGAC 305 3.4815106 32.720448 16
CGTAG 200 3.4607887 30.398376 17
TAGGC 200 3.4607887 38.571686 3
GGACT 200 3.4607882 33.04075 5
CGCCT 2745 3.4523716 5.4338827 21
GCTGC 70 3.444032 31.327469 13
TGCCG 70 3.4440315 31.429804 17
TTGAG 370 3.4134994 31.144999 27
TGGAT 370 3.4134994 41.123775 4
GGCTT 130 3.4100876 29.397287 22
TGGCT 130 3.4100876 37.583874 4
AGGCC 105 3.4078565 46.741837 21
CGAAC 11595 3.3833327 4.6970744 28
GTGAA 555 3.3776448 27.505453 29
GAGAT 555 3.3776448 21.321732 16
CAGTG 195 3.374269 33.380836 27
CATGG 195 3.374269 24.901953 19
AGGTA 550 3.3472154 35.847557 4
CGCTC 2655 3.339179 4.80323 7
GAGAA 830 3.33213 26.85171 16
AACCG 11390 3.3235154 4.4622207 28
AGAGA 825 3.3120568 27.473196 15
ACGCC 3980 3.3020308 4.3510766 32
ATGGC 190 3.2877493 30.43572 19
GTTTG 235 3.286572 26.816404 18
AACGA 31800 3.2634506 3.9924846 22
ATCCG 7315 3.2356794 4.41465 28
CGATA 20550 3.1969728 4.3337455 22
ATGGT 345 3.1828582 24.97138 3
TGAAG 520 3.1646402 25.2701 17
GTTGT 225 3.146718 26.816404 18
AGGAC 275 3.139067 29.075333 4
TGAGT 340 3.1367292 26.731544 28
TAAGG 515 3.1342113 26.274265 19
GAGTA 515 3.1342108 23.576101 29
ACGAA 30485 3.1284997 3.868759 24
CTTCG 4660 3.124742 5.129572 12
CTGGA 180 3.1147099 35.816566 3
GGCTA 180 3.1147094 24.933004 22
GGCAT 180 3.1147094 44.32534 22
CACCG 3745 3.1070616 4.884799 7
AGGAT 510 3.1037817 29.211727 20
TTTCG 8680 3.103138 3.998742 18
GTGAT 335 3.0906007 22.037796 16
AAACG 30100 3.0889893 3.744174 21
CCGTA 6975 3.0852857 4.65517 30
CGCAG 95 3.083299 25.862686 12
CCAGG 95 3.0832987 41.48833 18
AGCCG 95 3.0832982 41.46623 17
TCGCT 4585 3.0744514 4.0598884 9
TGAGA 505 3.0733523 29.05622 15
CGCCA 3650 3.0282445 5.0474114 22
AGGCT 175 3.0281897 30.454586 20
TCGTC 4500 3.0174549 4.3056803 26
TAGGA 495 3.0124943 30.166748 19
ACGAC 10305 3.0069206 3.6943822 31
CAGAG 260 2.9678454 23.616644 14
TGGTA 320 2.9522161 22.141367 20
GCCTG 60 2.9520278 15.772161 23
GAGCC 90 2.9210198 25.842873 16
CGTCT 4340 2.9101677 5.0227065 12
TGTGC 110 2.885459 42.225582 28
TAGAG 470 2.8603482 24.214075 14
TGTGA 310 2.8599594 16.335947 28
AGTGA 460 2.7994893 26.45076 28
CAGGT 160 2.768631 30.306324 3
GCAGC 85 2.7587411 21.072945 31
GCTGT 105 2.7543018 25.148813 18
CTGCG 55 2.7060254 31.321032 14
CTGGC 55 2.7060251 23.75986 28
CGTTT 7455 2.665195 5.2343903 15
AGATG 430 2.616914 28.18588 17
GTGAC 150 2.595591 33.51707 29
TGAGC 150 2.595591 27.538605 15
TGACG 150 2.595591 19.34442 17
AGGCA 220 2.5112536 23.623709 4
CTAGG 145 2.5090718 22.119692 18
AGAAG 605 2.4288416 21.79894 17
TGCAG 140 2.4225516 27.634886 17
ATGAG 395 2.4039097 24.214075 14
AAGGC 210 2.397106 19.991995 3
AGATC 15165 2.3592255 13.156048 32
GATGT 255 2.352547 22.462734 31
GTATG 255 2.352547 22.03066 1
GAGTC 135 2.3360322 36.31016 29
GTTGC 85 2.229673 29.229166 13
GTAGT 240 2.2141619 20.638123 18
GATGC 125 2.1629927 14.043919 31
GTTGA 230 2.1219053 22.112276 18
GATTG 230 2.121905 20.56195 1
GTAAG 345 2.099617 24.311121 18
TGCTG 80 2.0985155 16.756943 17
AGACG 180 2.054662 20.052715 17
AGTTG 215 1.9835199 30.940693 17
GAAGT 325 1.9779 22.281528 13
GTTAG 210 1.9373915 26.534729 18
GTCAG 110 1.9034336 30.30232 1
TGATG 205 1.8912631 20.627129 17
AGTCG 105 1.8169138 14.008322 30
GACTG 100 1.7303941 19.283295 1
GCAAG 150 1.7122185 14.537712 1
GAAGC 150 1.7122184 16.353344 13
GATGA 275 1.6736077 13.56267 13
AGTAG 275 1.6736077 18.428339 12
GCAGT 95 1.6438746 22.035923 13
GCCAG 50 1.6227889 15.558126 18
GCTTG 60 1.5738868 25.055992 1
GTCTG 60 1.5738866 20.957342 18
GTAGC 90 1.5573547 19.281431 13
GAATG 255 1.5518909 17.504007 18
GCAGA 130 1.4839227 9.119729 18
GATAG 230 1.3997446 17.504007 18
GCATG 80 1.3843155 22.038054 1
GCTGA 75 1.2977958 13.772451 13
AGCTG 75 1.2977955 16.546566 12
AGCAG 110 1.2556268 16.406767 17
GACAG 105 1.1985528 12.720497 1
GCTTC 1755 1.1768074 7.6950436 11
GCTAG 45 0.77867746 13.824808 18
TAGAT 8965 0.7435847 6.2801623 32
Produced by FastQC (version 0.10.1)