##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	filtered_Y54_Mix_GTGGCC_L005_R1.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	15646291	
Filtered Sequences	0	
Sequence length	36	
%GC	24	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.919259075521477	33.0	33.0	34.0	30.0	34.0
2	32.45101653804087	34.0	33.0	34.0	31.0	34.0
3	33.0455074624395	34.0	33.0	34.0	31.0	34.0
4	36.20633420406153	37.0	37.0	37.0	35.0	37.0
5	35.73590584503382	37.0	37.0	37.0	35.0	37.0
6	35.72355525025068	37.0	37.0	37.0	35.0	37.0
7	35.44653279170124	37.0	37.0	37.0	35.0	37.0
8	34.99854227433198	37.0	35.0	37.0	32.0	37.0
9	37.2274908475114	39.0	39.0	39.0	35.0	39.0
10	37.081763531050264	39.0	39.0	39.0	35.0	39.0
11	37.31492505156653	39.0	39.0	39.0	35.0	39.0
12	36.97404867389977	39.0	39.0	39.0	34.0	39.0
13	36.87473018365822	39.0	38.0	39.0	32.0	39.0
14	38.46871248911324	41.0	39.0	41.0	34.0	41.0
15	38.64151638238098	41.0	39.0	41.0	34.0	41.0
16	38.845473601379396	41.0	39.0	41.0	36.0	41.0
17	39.155116570438324	41.0	39.0	41.0	37.0	41.0
18	39.002972972955696	40.0	39.0	41.0	36.0	41.0
19	39.24832645641066	41.0	39.0	41.0	37.0	41.0
20	39.44157244678627	41.0	40.0	41.0	38.0	41.0
21	39.61105203782801	41.0	40.0	41.0	38.0	41.0
22	39.52194235681798	41.0	40.0	41.0	38.0	41.0
23	39.46599996126878	41.0	40.0	41.0	38.0	41.0
24	39.429183440343785	41.0	40.0	41.0	37.0	41.0
25	39.368769697559635	41.0	40.0	41.0	38.0	41.0
26	39.17114624801494	41.0	39.0	41.0	37.0	41.0
27	39.239334485086594	41.0	40.0	41.0	37.0	41.0
28	39.21599681355792	41.0	40.0	41.0	37.0	41.0
29	39.28576836516718	41.0	40.0	41.0	37.0	41.0
30	38.981664664168655	40.0	39.0	41.0	37.0	41.0
31	38.92154472903514	40.0	39.0	41.0	37.0	41.0
32	38.91504344384238	40.0	39.0	41.0	37.0	41.0
33	38.91917624438916	40.0	39.0	41.0	37.0	41.0
34	38.85928351965332	40.0	39.0	41.0	37.0	41.0
35	38.354902002014406	40.0	38.0	41.0	35.0	41.0
36	37.88652665350529	39.0	38.0	40.0	35.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	764.0
3	110.0
4	5328.0
5	1716.0
6	2482.0
7	1550.0
8	1742.0
9	1986.0
10	2280.0
11	2426.0
12	2880.0
13	3600.0
14	3981.0
15	4675.0
16	5577.0
17	6613.0
18	7782.0
19	9910.0
20	12055.0
21	13765.0
22	16717.0
23	20523.0
24	24419.0
25	29767.0
26	37189.0
27	46456.0
28	61024.0
29	87649.0
30	138708.0
31	218917.0
32	323728.0
33	347512.0
34	374342.0
35	444195.0
36	575691.0
37	1199332.0
38	5297250.0
39	6311650.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.14972702120348	34.13433724504431	30.512893600830466	0.20304213292175205
2	13.496076954979197	45.66020420250777	40.69580672834138	0.14791211417164996
3	15.972303357185094	26.670085626537603	57.16370307100485	0.19390794527244004
4	16.149616544905115	28.712287148436648	42.503312765945616	12.634783540712622
5	28.987323577197945	28.87213972947327	41.82975377359401	0.31078291973477934
6	29.62670833618012	29.19600562203528	40.854666450981895	0.3226195908027021
7	17.582115787057777	39.75434817107774	42.33832797817706	0.325208063687426
8	16.999198084709022	40.19784624995151	42.44233345781438	0.36062220752509333
9	29.882570891721237	26.98874768467492	42.729590035108004	0.3990913884958422
10	17.592779244027177	40.12994149730943	41.9412278496174	0.33605140904600106
11	29.26538907615242	27.422748307118216	42.89972021895196	0.412142397777398
12	16.71000494622016	27.718722603331358	42.90790705605565	12.66336539439283
13	16.670404513896322	40.08030416979494	42.842293120381925	0.4069981959268096
14	16.67483484681147	27.637357088804354	43.14271689777887	12.54509116660531
15	17.14344952423549	40.19845981389455	42.263684089730916	0.3944065721390456
16	17.583394045272456	27.821034390834225	42.16086099894218	12.434710564951144
17	30.123950781690052	27.404405299632995	42.12682737397636	0.34481654470059386
18	17.948775961990215	27.08130050279661	54.564370447761824	0.4055530874513551
19	17.950879081266123	26.977031653061378	42.668485249791	12.403604015881498
20	17.95997530660781	27.093890814123295	54.55402817191627	0.39210570735262434
21	30.062300387996103	27.227187580749966	42.30712569515676	0.4033863360971619
22	17.497622919067528	39.56478886913199	42.56516128966283	0.3724269221376491
23	17.54039343893067	39.79447908772757	42.21141611133271	0.45371136200905376
24	17.489226826684337	27.56036709575291	42.547036124643625	12.403369952919137
25	17.662677348961576	27.537042378530096	54.30419179710142	0.4960884754069055
26	17.77126656746459	27.518215630679904	42.249046380528206	12.461471421327303
27	17.951736774444598	27.397797347733466	42.17806742181461	12.472398456007323
28	18.385611470691675	39.34476621823109	41.77870737900734	0.4909149320698916
29	30.448454827266623	27.094972995429995	42.077920168738615	0.37865200856476305
30	18.104982035811794	27.067671287441584	54.33905516525698	0.4882915114896407
31	17.894999121401366	27.116551781931626	42.57768886179193	12.41076023487508
32	17.74047044820998	39.472859961787826	42.21789119694609	0.568778393056098
33	17.75378582119978	27.23797153752982	42.50360649509778	12.504636146172615
34	29.94818443559434	27.359419475473505	42.193318481651595	0.49907760728056233
35	17.962413615165374	27.571414361323733	54.01960852001356	0.44656350349732965
36	30.35092229093018	27.56674184546131	41.663072622613356	0.41926324099516205
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	35.352769154125234
2	13.643989069150846
3	16.166211302457533
4	28.784400085617733
5	29.298106496932725
6	29.94932792698282
7	17.9073238507452
8	17.359820292234115
9	30.28166228021708
10	17.928830653073177
11	29.677531473929818
12	29.373370340612993
13	17.077402709823133
14	29.21992601341678
15	17.537856096374536
16	30.0181046102236
17	30.468767326390644
18	18.35432904944157
19	30.35448309714762
20	18.352081013960433
21	30.46568672409327
22	17.870049841205176
23	17.994104800939724
24	29.892596779603476
25	18.158765824368484
26	30.232737988791897
27	30.42413523045192
28	18.876526402761566
29	30.82710683583139
30	18.593273547301433
31	30.305759356276447
32	18.309248841266076
33	30.258421967372396
34	30.4472620428749
35	18.408977118662705
36	30.770185531925335
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8626.0
1	31244.0
2	53862.0
3	53862.0
4	141426.0
5	228990.0
6	228990.0
7	420429.5
8	611869.0
9	611869.0
10	888955.5
11	1166042.0
12	1166042.0
13	1440281.0
14	1714520.0
15	1877120.5
16	2039721.0
17	2039721.0
18	2037019.0
19	2034317.0
20	2034317.0
21	1896677.5
22	1759038.0
23	1759038.0
24	1558524.5
25	1358011.0
26	1161427.5
27	964844.0
28	964844.0
29	804201.5
30	643559.0
31	643559.0
32	525111.5
33	406664.0
34	406664.0
35	329314.0
36	251964.0
37	251964.0
38	204149.5
39	156335.0
40	125654.0
41	94973.0
42	94973.0
43	76534.0
44	58095.0
45	58095.0
46	48978.5
47	39862.0
48	39862.0
49	66131.0
50	92400.0
51	259037.5
52	425675.0
53	425675.0
54	933666.0
55	1441657.0
56	1441657.0
57	759928.5
58	78200.0
59	78200.0
60	44507.0
61	10814.0
62	10814.0
63	6912.5
64	3011.0
65	2206.5
66	1402.0
67	1402.0
68	1050.5
69	699.0
70	699.0
71	577.0
72	455.0
73	455.0
74	365.5
75	276.0
76	226.5
77	177.0
78	177.0
79	131.0
80	85.0
81	85.0
82	73.5
83	62.0
84	62.0
85	47.5
86	33.0
87	33.0
88	28.0
89	23.0
90	17.5
91	12.0
92	12.0
93	11.5
94	11.0
95	11.0
96	7.5
97	4.0
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08676177632130196
2	0.07674023191822266
3	0.001322997252192229
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.001105693355696887
11	0.003099776170595319
12	0.0
13	1.917387322017723E-4
14	0.0013485624164857985
15	0.0
16	0.0
17	0.0
18	2.556516429356964E-5
19	0.0026268206311642803
20	0.0
21	0.0
22	0.0
23	0.0
24	1.2143453039445579E-4
25	5.752161966053169E-4
26	7.222158912933423E-4
27	0.00562433614458532
28	0.011689671373234718
29	0.0050555112390533955
30	0.004595338281769143
31	0.013166059611188365
32	0.013172450902261757
33	0.0013677362897059756
34	1.7256485898159508E-4
35	0.02236312746580004
36	2.2369518756873435E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	1.5646291E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	20.437012263099213
#Duplication Level	Relative count
1	100.0
2	5.536374845869297
3	1.5850241004371708
4	0.9298284945633898
5	0.6389418226656205
6	0.502185853603856
7	0.374957964353772
8	0.2768747898217689
9	0.24829055038672795
10++	2.0025781863019843
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG	1381586	8.830118268923925	TruSeq Adapter, Index 1 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGTG	49951	0.3192513804070243	TruSeq Adapter, Index 6 (97% over 34bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGTG	48686	0.3111663971991829	TruSeq Adapter, Index 6 (97% over 34bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGTG	45269	0.289327355601401	TruSeq Adapter, Index 6 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGTG	43450	0.2777015971389002	TruSeq Adapter, Index 6 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGTG	42542	0.27189830484425986	TruSeq Adapter, Index 6 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGTG	41721	0.2666510548730047	TruSeq Adapter, Index 6 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGTG	37664	0.2407215869882517	TruSeq Adapter, Index 6 (97% over 34bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACGTG	35765	0.22858452523987954	TruSeq Adapter, Index 6 (97% over 34bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGTG	17209	0.10998772808200998	TruSeq Adapter, Index 6 (97% over 34bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACGTG	15952	0.10195387520275571	TruSeq Adapter, Index 6 (97% over 34bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CACAC	1825320	710.728	22436.059	12
ACTCC	1785300	493.95657	15508.486	23
CTCCA	1781605	492.93423	15469.8	24
CACGT	3687250	182.30687	2881.7546	14
GCACA	1883240	131.03673	4137.574	11
AGCAC	1878470	130.7048	4126.4507	10
ACACG	1845165	128.38745	4051.1619	13
TCACG	1857085	91.81893	2868.6235	30
GTCAC	1853080	91.62092	2866.6685	29
CAGTC	1846480	91.2946	2857.7798	27
ACGTC	1835500	90.751724	2855.073	15
TCCAG	1834325	90.69362	2845.561	25
CCAGT	1834155	90.68522	2840.557	26
CGTCT	1832005	64.36358	2027.7239	16
AACTC	1834885	60.7265	1907.1091	22
CGGAA	1972000	24.519815	759.80975	4
AGAGC	1931860	24.020714	755.8736	8
GAGCA	1925800	23.945364	756.0165	9
ATCGG	2016390	17.815495	543.9166	2
TCGGA	2006050	17.724136	543.1497	3
GATCG	2001895	17.687426	543.79395	1
ACGTG	1911370	16.88761	524.41223	32
GTCTG	1890635	11.869821	373.81787	17
TGAAC	1904380	11.262789	352.41702	20
GAACT	1899705	11.23514	352.89444	21
AGTCA	1897340	11.221152	351.10437	28
CTGAA	1889835	11.176767	351.29828	19
TCTGA	1888085	7.934634	249.60886	18
CCCCC	290	7.8918486	52.242413	16
CTCCC	2965	6.858204	209.83524	24
CCCAC	1800	5.859291	159.34943	12
GGAAG	2346290	5.2133255	138.09682	5
CACGC	7380	4.2929106	102.17728	12
CGCAC	7105	4.132944	101.15365	12
GAAGA	2547580	3.7890465	92.168144	6
AAGAG	2466455	3.6683884	91.99624	7
CACCC	1105	3.5969536	83.31996	12
CCTCC	1520	3.5158417	54.401733	24
GGGGG	625045	3.0996063	5.2024336	1
CTCCG	6320	2.6123157	81.211334	24
CACGG	23630	2.456304	56.078297	31
CCCAG	3545	2.0621095	47.18873	26
CACAT	60015	1.9862285	60.648636	12
ACCCC	565	1.8391664	35.41258	23
GCTCC	4365	1.8042339	54.88615	23
CGCGT	24010	1.7734692	14.167929	14
CCACG	2990	1.7392687	29.406214	13
TCGCG	23445	1.7317362	13.520477	30
CCCCA	505	1.6438569	35.936134	24
CGTCG	22120	1.6338667	14.23901	16
ACGCG	15115	1.5711823	19.240143	13
GCACG	15025	1.5618268	19.889328	11
ACTTC	65610	1.5429524	47.119606	23
CATAC	46415	1.5361292	48.222416	12
CCGTC	3650	1.508695	13.820256	15
CTTCA	63905	1.5028559	46.92383	24
GGCAC	14330	1.4895825	35.322212	10
TTCCA	55310	1.300727	40.437046	24
ATTCC	55020	1.293907	40.494118	23
ACTCT	54225	1.2752111	39.594917	23
CTCTA	53545	1.2592195	39.481335	24
CGCCC	250	1.2157482	17.115961	10
TGGCC	15295	1.1297463	14.678699	30
GCCCA	1795	1.044143	30.244707	11
CGTCC	2520	1.0416195	30.946795	16
AGCCC	1670	0.97143096	27.54623	10
CCCTC	395	0.91365623	19.611822	22
AGCGC	8520	0.8856415	17.927185	10
GGCCT	11600	0.8568198	14.764258	31
CTCAC	3050	0.8438736	17.79345	12
GCCAC	1440	0.83764106	16.846823	29
CCACC	255	0.83006626	6.249199	11
ACGGC	7810	0.8118381	18.95765	15
ACGCC	1380	0.8027394	16.099695	23
CCCCG	165	0.80239385	8.557618	13
GCCCC	160	0.77807885	7.7812185	29
TACGT	184620	0.77586144	7.60517	31
CGGAC	7405	0.769739	6.485352	4
CGGTC	10300	0.76079684	13.686971	27
TCCCA	2700	0.7470357	22.710253	25
GCGCA	7165	0.74479127	18.592197	11
CCAGC	1235	0.71839356	20.569445	26
GCGTC	9720	0.7179559	13.530013	15
TACAC	21550	0.7132088	21.569841	12
CGTGG	52615	0.6944868	6.8037853	32
CCCGT	1680	0.69441307	11.439588	14
GCACC	1145	0.666041	17.867643	11
CGTTT	220285	0.65781456	6.4311604	16
CACTC	2320	0.6418973	5.134428	12
ACGTT	152185	0.63955414	8.862332	15
ACGGG	33985	0.63128954	10.600543	32
TTACG	149905	0.62997234	7.706593	30
GTCGC	8520	0.6293193	12.409799	29
ATACG	104160	0.6160178	9.039285	13
CAGCC	1005	0.5846037	17.126972	27
ACACC	1465	0.5704296	10.340202	13
CGCCA	965	0.56133586	16.100943	24
CGCTC	1270	0.5249432	13.764512	2
CACCG	830	0.48280704	8.840459	14
ACCCG	825	0.47989857	13.772531	13
CATGT	109790	0.46139002	7.7865424	14
ACCTC	1665	0.460672	6.68391	23
TCCGG	6020	0.44465992	13.543881	25
CCGGT	5960	0.4402281	13.248658	26
CGGCT	5910	0.4365349	13.222782	16
CGAGC	4165	0.43294567	5.903584	8
AACCC	1080	0.4205215	11.025393	22
GCCTT	11325	0.3978797	7.027956	32
ACCAC	975	0.37963745	5.6063724	10
TCCCG	875	0.36167347	5.2908516	25
ACATG	60715	0.3590776	10.930591	13
CCAGG	3135	0.32587868	9.896239	26
CTTCC	1650	0.3243953	6.416169	6
CAGGC	3115	0.3237997	9.913612	27
GTTAC	76110	0.31985056	7.394752	29
ATGTC	74940	0.31493372	7.761095	15
ACCCA	805	0.31344426	6.914543	23
TAGTC	74380	0.31258032	7.327903	27
AGCTC	6305	0.311735	6.7311053	22
GACTC	5970	0.29517174	8.233937	22
GCATA	48970	0.28961593	8.853185	11
TCAGT	68710	0.28875223	8.6184	26
AGCAT	48610	0.2874868	8.84665	10
AGGGC	15365	0.28541306	6.4546113	8
TTCAG	67415	0.28331	8.633038	25
AATTC	89050	0.25050032	5.048762	22
CAGTT	56605	0.23788129	7.1792874	27
TCTAG	56500	0.23743998	7.2646885	25
CTAGT	56440	0.23718783	7.2412844	26
GAGCC	2130	0.2214104	6.302714	9
GGGCA	11820	0.21956281	6.5853834	9
TCATG	51150	0.21495672	6.482118	30
GTCAT	50965	0.21417928	6.4762077	29
AACTT	68660	0.19314265	5.782293	22
CGCCT	460	0.19013691	5.2900505	16
TGTCT	62055	0.18530849	5.491949	16
>>END_MODULE