FastQCFastQC Report
Tue 3 Jun 2014
filtered_Y54_Mix_GTGGCC_L005_R1.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename filtered_Y54_Mix_GTGGCC_L005_R1.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 15646291
Filtered Sequences 0
Sequence length 36
%GC 24

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG 1381586 8.830118268923925 TruSeq Adapter, Index 1 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGTG 49951 0.3192513804070243 TruSeq Adapter, Index 6 (97% over 34bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGTG 48686 0.3111663971991829 TruSeq Adapter, Index 6 (97% over 34bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGTG 45269 0.289327355601401 TruSeq Adapter, Index 6 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGTG 43450 0.2777015971389002 TruSeq Adapter, Index 6 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGTG 42542 0.27189830484425986 TruSeq Adapter, Index 6 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGTG 41721 0.2666510548730047 TruSeq Adapter, Index 6 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGTG 37664 0.2407215869882517 TruSeq Adapter, Index 6 (97% over 34bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACGTG 35765 0.22858452523987954 TruSeq Adapter, Index 6 (97% over 34bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGTG 17209 0.10998772808200998 TruSeq Adapter, Index 6 (97% over 34bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACGTG 15952 0.10195387520275571 TruSeq Adapter, Index 6 (97% over 34bp)

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CACAC 1825320 710.728 22436.059 12
ACTCC 1785300 493.95657 15508.486 23
CTCCA 1781605 492.93423 15469.8 24
CACGT 3687250 182.30687 2881.7546 14
GCACA 1883240 131.03673 4137.574 11
AGCAC 1878470 130.7048 4126.4507 10
ACACG 1845165 128.38745 4051.1619 13
TCACG 1857085 91.81893 2868.6235 30
GTCAC 1853080 91.62092 2866.6685 29
CAGTC 1846480 91.2946 2857.7798 27
ACGTC 1835500 90.751724 2855.073 15
TCCAG 1834325 90.69362 2845.561 25
CCAGT 1834155 90.68522 2840.557 26
CGTCT 1832005 64.36358 2027.7239 16
AACTC 1834885 60.7265 1907.1091 22
CGGAA 1972000 24.519815 759.80975 4
AGAGC 1931860 24.020714 755.8736 8
GAGCA 1925800 23.945364 756.0165 9
ATCGG 2016390 17.815495 543.9166 2
TCGGA 2006050 17.724136 543.1497 3
GATCG 2001895 17.687426 543.79395 1
ACGTG 1911370 16.88761 524.41223 32
GTCTG 1890635 11.869821 373.81787 17
TGAAC 1904380 11.262789 352.41702 20
GAACT 1899705 11.23514 352.89444 21
AGTCA 1897340 11.221152 351.10437 28
CTGAA 1889835 11.176767 351.29828 19
TCTGA 1888085 7.934634 249.60886 18
CCCCC 290 7.8918486 52.242413 16
CTCCC 2965 6.858204 209.83524 24
CCCAC 1800 5.859291 159.34943 12
GGAAG 2346290 5.2133255 138.09682 5
CACGC 7380 4.2929106 102.17728 12
CGCAC 7105 4.132944 101.15365 12
GAAGA 2547580 3.7890465 92.168144 6
AAGAG 2466455 3.6683884 91.99624 7
CACCC 1105 3.5969536 83.31996 12
CCTCC 1520 3.5158417 54.401733 24
GGGGG 625045 3.0996063 5.2024336 1
CTCCG 6320 2.6123157 81.211334 24
CACGG 23630 2.456304 56.078297 31
CCCAG 3545 2.0621095 47.18873 26
CACAT 60015 1.9862285 60.648636 12
ACCCC 565 1.8391664 35.41258 23
GCTCC 4365 1.8042339 54.88615 23
CGCGT 24010 1.7734692 14.167929 14
CCACG 2990 1.7392687 29.406214 13
TCGCG 23445 1.7317362 13.520477 30
CCCCA 505 1.6438569 35.936134 24
CGTCG 22120 1.6338667 14.23901 16
ACGCG 15115 1.5711823 19.240143 13
GCACG 15025 1.5618268 19.889328 11
ACTTC 65610 1.5429524 47.119606 23
CATAC 46415 1.5361292 48.222416 12
CCGTC 3650 1.508695 13.820256 15
CTTCA 63905 1.5028559 46.92383 24
GGCAC 14330 1.4895825 35.322212 10
TTCCA 55310 1.300727 40.437046 24
ATTCC 55020 1.293907 40.494118 23
ACTCT 54225 1.2752111 39.594917 23
CTCTA 53545 1.2592195 39.481335 24
CGCCC 250 1.2157482 17.115961 10
TGGCC 15295 1.1297463 14.678699 30
GCCCA 1795 1.044143 30.244707 11
CGTCC 2520 1.0416195 30.946795 16
AGCCC 1670 0.97143096 27.54623 10
CCCTC 395 0.91365623 19.611822 22
AGCGC 8520 0.8856415 17.927185 10
GGCCT 11600 0.8568198 14.764258 31
CTCAC 3050 0.8438736 17.79345 12
GCCAC 1440 0.83764106 16.846823 29
CCACC 255 0.83006626 6.249199 11
ACGGC 7810 0.8118381 18.95765 15
ACGCC 1380 0.8027394 16.099695 23
CCCCG 165 0.80239385 8.557618 13
GCCCC 160 0.77807885 7.7812185 29
TACGT 184620 0.77586144 7.60517 31
CGGAC 7405 0.769739 6.485352 4
CGGTC 10300 0.76079684 13.686971 27
TCCCA 2700 0.7470357 22.710253 25
GCGCA 7165 0.74479127 18.592197 11
CCAGC 1235 0.71839356 20.569445 26
GCGTC 9720 0.7179559 13.530013 15
TACAC 21550 0.7132088 21.569841 12
CGTGG 52615 0.6944868 6.8037853 32
CCCGT 1680 0.69441307 11.439588 14
GCACC 1145 0.666041 17.867643 11
CGTTT 220285 0.65781456 6.4311604 16
CACTC 2320 0.6418973 5.134428 12
ACGTT 152185 0.63955414 8.862332 15
ACGGG 33985 0.63128954 10.600543 32
TTACG 149905 0.62997234 7.706593 30
GTCGC 8520 0.6293193 12.409799 29
ATACG 104160 0.6160178 9.039285 13
CAGCC 1005 0.5846037 17.126972 27
ACACC 1465 0.5704296 10.340202 13
CGCCA 965 0.56133586 16.100943 24
CGCTC 1270 0.5249432 13.764512 2
CACCG 830 0.48280704 8.840459 14
ACCCG 825 0.47989857 13.772531 13
CATGT 109790 0.46139002 7.7865424 14
ACCTC 1665 0.460672 6.68391 23
TCCGG 6020 0.44465992 13.543881 25
CCGGT 5960 0.4402281 13.248658 26
CGGCT 5910 0.4365349 13.222782 16
CGAGC 4165 0.43294567 5.903584 8
AACCC 1080 0.4205215 11.025393 22
GCCTT 11325 0.3978797 7.027956 32
ACCAC 975 0.37963745 5.6063724 10
TCCCG 875 0.36167347 5.2908516 25
ACATG 60715 0.3590776 10.930591 13
CCAGG 3135 0.32587868 9.896239 26
CTTCC 1650 0.3243953 6.416169 6
CAGGC 3115 0.3237997 9.913612 27
GTTAC 76110 0.31985056 7.394752 29
ATGTC 74940 0.31493372 7.761095 15
ACCCA 805 0.31344426 6.914543 23
TAGTC 74380 0.31258032 7.327903 27
AGCTC 6305 0.311735 6.7311053 22
GACTC 5970 0.29517174 8.233937 22
GCATA 48970 0.28961593 8.853185 11
TCAGT 68710 0.28875223 8.6184 26
AGCAT 48610 0.2874868 8.84665 10
AGGGC 15365 0.28541306 6.4546113 8
TTCAG 67415 0.28331 8.633038 25
AATTC 89050 0.25050032 5.048762 22
CAGTT 56605 0.23788129 7.1792874 27
TCTAG 56500 0.23743998 7.2646885 25
CTAGT 56440 0.23718783 7.2412844 26
GAGCC 2130 0.2214104 6.302714 9
GGGCA 11820 0.21956281 6.5853834 9
TCATG 51150 0.21495672 6.482118 30
GTCAT 50965 0.21417928 6.4762077 29
AACTT 68660 0.19314265 5.782293 22
CGCCT 460 0.19013691 5.2900505 16
TGTCT 62055 0.18530849 5.491949 16
Produced by FastQC (version 0.10.1)