FastQCFastQC Report
Tue 3 Jun 2014
filtered_Y54_Mix_GTGGCC_L005_R1_tr.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename filtered_Y54_Mix_GTGGCC_L005_R1_tr.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 13412322
Filtered Sequences 0
Sequence length 19-36
%GC 20

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CCCCC 85 224267.55 1675971.4 16
CTCCC 2065 44247.246 1327930.1 24
CCTCC 685 14677.658 372093.53 24
CACAC 17180 6704.2437 157622.78 12
CACCC 180 5775.766 117176.39 12
ACTCC 15090 3932.2852 44837.25 24
CCCAC 120 3850.5107 56040.883 12
CCCCA 115 3690.073 40896.535 24
CTCCA 13325 3472.3457 49357.105 25
ACCCC 105 3369.197 30656.361 23
CCCTC 150 3214.0857 51149.855 22
CGCCC 55 2859.0513 24765.07 16
GCCCC 55 2859.0513 17055.518 30
CCCGC 40 2079.31 16590.621 26
CCACC 60 1925.2554 15284.276 7
CCCAG 2500 1580.4792 39243.867 26
CCCCG 30 1559.4824 16519.25 1
CCGCC 25 1299.5687 9012.106 32
CCCCT 50 1071.3619 7407.917 31
CGCGC 890 911.509 2276.536 5
CCGTC 1680 709.2301 11732.08 16
CGTCC 1430 603.68994 18637.248 16
CGCTC 1285 542.4766 16098.653 2
TCCCA 2050 534.20703 16203.091 25
TCCCC 20 428.54477 3515.1335 30
CACGC 650 410.92462 9435.212 14
CACCG 580 366.6712 8732.59 14
CTTCC 1625 282.77313 6548.0 6
AACCC 630 245.84831 6396.6245 22
ACACC 615 239.99478 6505.6567 13
CCACG 360 227.58902 3111.6138 12
GCACC 360 227.58902 6223.3853 11
ACCCA 560 218.53185 5903.1323 23
ACCTC 665 173.29155 4647.357 23
CACGT 33275 170.83841 1830.0326 14
CTCCG 400 168.8643 4506.2173 24
CACTC 645 168.07977 3268.5706 14
GCCAC 265 167.5308 4416.5957 11
CGGCG 8030 162.03104 221.57396 22
TCGCG 17730 147.46817 183.98878 22
AGCAC 19005 146.11887 3457.147 10
CGCAC 230 145.4041 1907.1182 12
CGCGG 7140 144.07243 215.0355 21
CGTCG 16955 141.02216 319.64215 16
GCACA 18320 140.85226 3348.5005 11
CGCGA 10490 130.65799 184.3446 22
ACACG 16655 128.05103 2906.5178 13
GCGCG 5755 116.12561 150.63472 17
CGACG 9255 115.275475 174.02783 6
ACGCG 8595 107.054855 148.31906 13
CGCGT 12650 105.21559 146.72421 19
ACGTC 20210 103.76091 1697.1731 15
ACCAC 245 95.607666 1363.09 13
TCACG 17370 89.17996 1513.1841 31
CCAGC 140 88.50684 1916.7955 26
GTCAC 17060 87.58838 1411.6177 30
CAGTC 16945 86.99795 1373.3223 28
CCCGT 205 86.54296 1742.7146 14
ACCCG 135 85.34588 2308.6167 13
CCATC 320 83.38841 2245.5508 26
CCAGT 16025 82.27454 1355.0427 27
CGGCC 80 81.93339 650.564 16
CCACA 185 72.19355 929.3796 12
GCTCC 170 71.767334 604.99615 23
TCCAG 13745 70.56871 1038.0481 26
CACCA 180 70.24238 1239.1727 12
CGGAC 5350 66.63683 96.9018 4
ACGCC 105 66.38013 805.3247 23
GCCCA 85 53.736298 1104.149 11
CGTCT 15545 53.295094 926.1044 16
AACTC 16270 51.56237 561.1544 23
AGCCC 80 50.575336 803.01746 10
GCCCT 115 48.54849 805.77136 21
CTCGC 110 46.437687 336.28488 24
TGCCC 110 46.437683 872.5451 20
GGCCC 45 46.087532 354.07962 31
TCCCG 105 44.32688 605.7562 25
CTGCC 105 44.32688 1073.463 19
CTCAC 160 41.694206 537.8663 12
CCAAC 105 40.974716 248.27861 21
CCCGG 40 40.966694 325.21906 14
GCCCG 40 40.966694 488.01907 17
GCGCC 40 40.966694 487.8415 9
CTCTC 230 40.023277 499.01862 24
CACCT 150 39.088318 620.6147 14
GCGGC 1860 37.531475 67.435555 22
TCGGC 4510 37.511646 54.48149 27
CCCAA 95 37.072365 309.9936 18
CGGGC 1800 36.32078 70.60867 21
CCGGC 35 35.84586 325.282 16
CGGTC 4220 35.09959 105.666824 16
CGCCA 55 34.770546 705.02795 24
GCGTC 4125 34.309433 71.31178 15
TTCGC 9995 34.267254 44.716015 21
CGTTC 9615 32.964447 50.089123 16
TCCAC 125 32.5736 207.73193 25
CGACC 50 31.609587 301.1239 4
CCGCG 30 30.725021 328.20282 29
CGATC 5920 30.39409 220.09866 10
TCGTC 8860 30.375975 40.101513 29
ACCCT 115 29.96771 135.53268 32
CGCCT 70 29.551254 201.12137 16
CAACC 75 29.267656 124.13932 21
ACCGC 45 28.448627 202.2775 28
ATCGC 5435 27.904037 48.94622 2
CGAGC 2185 27.215225 67.23974 8
ACGGC 2180 27.152948 63.283203 15
GGCGC 1345 27.139694 48.057384 10
ACGAC 3465 26.640455 37.909916 21
GTCGC 3195 26.574213 47.75658 30
CCACT 100 26.05888 124.75282 26
CAGCC 40 25.287668 303.00204 27
TCGAC 4765 24.464163 36.71717 19
CGTAC 4735 24.310139 36.709663 2
TACCC 90 23.452993 135.13785 31
CCCTG 55 23.218843 134.18288 19
TTCCC 130 22.621851 277.23257 24
AGCGC 1775 22.108479 43.508064 8
TACGC 4300 22.076792 37.497345 4
AACGC 2855 21.950504 32.98057 18
TCTCC 125 21.75178 248.99695 20
CTCCT 125 21.75178 388.1256 24
CCTCA 80 20.847103 166.18555 25
CCTAC 80 20.847103 83.031944 24
CGCCG 20 20.483347 163.16628 24
GACGC 1625 20.240156 39.798435 27
CGAAC 2580 19.836184 158.84406 19
GCGAC 1585 19.74194 41.529373 5
CCTCT 110 19.141567 110.83503 23
GTCCC 45 18.997234 270.56973 29
CCGCA 30 18.965752 101.00069 27
CCCTA 70 18.241215 83.50781 29
TGCGC 2175 18.090425 30.477325 24
CGTGC 2100 17.46662 31.693949 5
TCACC 65 16.938272 85.49907 30
CCCAT 65 16.938272 82.74866 12
TCGCC 40 16.88643 201.23317 2
CCTGC 40 16.88643 67.25697 24
CCTCG 40 16.88643 134.23772 20
CCCGA 25 15.804793 301.31873 18
TCCTC 90 15.661282 83.03454 21
CATCC 60 15.635329 124.48278 23
GCCGC 15 15.362511 162.8312 20
TCCCT 75 13.051068 60.160965 31
CTACC 50 13.02944 165.65515 19
TCCGC 30 12.664824 73.18886 32
CCGCT 30 12.664824 69.25539 30
GACCC 20 12.643834 100.60876 22
CCTTC 70 12.180997 138.61629 24
ATCGG 104125 10.532567 67.5682 2
GATCG 99635 10.078387 66.94147 1
GAACC 1260 9.687438 277.59842 21
CGGAA 63660 9.643102 91.89722 4
ACGCT 1850 9.498154 200.67978 1
CCGAC 15 9.482876 100.47037 19
TCGGA 89925 9.096191 65.33436 3
CAACG 1145 8.8032675 126.95416 12
GCACG 705 8.781114 132.50172 11
CCCTT 50 8.700712 55.487133 25
CCTGA 1570 8.060595 228.4624 19
CGGGG 20225 8.040497 10.824871 23
TCCGA 1480 7.5985227 197.27379 8
CCGAT 1450 7.4444985 198.08894 9
GGCAC 595 7.411012 88.99377 9
CACGG 580 7.224179 168.09488 14
GCAAC 935 7.188694 195.31905 11
GCCGT 810 6.737125 126.776505 15
GGCGG 16930 6.730562 8.473287 21
TGCCG 805 6.6955376 126.77566 14
GACGG 26740 6.5619607 7.909605 7
CTCAG 1275 6.546025 174.2234 24
GCCTC 15 6.332412 67.07783 1
TCGGG 37390 6.1271253 7.57128 14
GCGGG 15100 6.0030413 7.647975 20
GGGCG 14645 5.822155 8.717151 1
TTTCG 199665 5.559262 6.327474 22
TTCGG 82275 5.5574574 6.3704443 14
GTCGG 33195 5.4396873 6.4852443 26
GGACG 22075 5.417176 6.7838693 18
GGTCG 32700 5.3585715 6.535459 1
CGGAG 21755 5.338649 7.8704367 4
CGAGG 21630 5.3079734 6.3302298 23
GTCCT 1545 5.296939 151.93062 17
TTCCG 1530 5.2455125 132.27852 7
ATCCC 20 5.2117763 42.74954 30
TCCTG 1515 5.194086 151.42491 18
TCGGT 76675 5.179192 6.177564 7
ACGGA 34000 5.150258 6.141253 20
CGGTA 50600 5.1183467 6.376116 8
GCGAG 20395 5.0049067 6.238042 18
GCTCT 1445 4.9540954 134.45439 3
TATCG 117815 4.912321 6.3288608 3
GTTCG 71500 4.8296347 5.5652447 28
TGGCC 575 4.7825265 74.982506 32
TCGTT 166675 4.6407223 5.298449 30
GGCGA 18905 4.639262 5.9651275 18
TCGAG 45670 4.619662 5.428532 8
AGCGG 18790 4.611041 5.844381 14
CGTGG 28090 4.603128 42.046524 32
TTCGT 165030 4.5949206 5.415957 29
CGGGT 27755 4.548231 5.463847 15
CGGGA 18155 4.4552135 5.533752 16
GCGGT 26905 4.408941 5.715136 28
CGGAT 43545 4.4047112 5.063914 25
CGAGA 28945 4.384536 5.342146 26
AGACG 28945 4.384536 6.065283 2
CGCTG 525 4.366655 118.87518 16
CGGTT 64620 4.364909 5.303294 29
GAGCG 17680 4.338649 5.535541 28
GCGGA 17635 4.327606 5.337904 15
ATGCC 830 4.2613335 78.255325 13
CGTTT 152965 4.2589955 4.933316 31
TACGG 42070 4.2555113 5.9102864 3
AGCGA 28070 4.2519927 5.3913627 2
TTCGA 101230 4.2208056 4.7862577 25
AGGCG 17170 4.2134953 5.1820526 15
GACGA 27765 4.205792 5.2199807 16
AGAGC 27205 4.120964 75.01567 8
GTCGA 40565 4.103275 6.1374373 17
TAGCG 40290 4.075458 4.966366 1
ACGGG 16580 4.0687103 5.2989073 4
ATCGT 97345 4.05882 4.4817743 4
GAGCC 325 4.0480313 104.81488 9
GTACG 39995 4.0456176 5.0949445 1
ATTCG 96395 4.0192094 4.624257 2
GTCGT 59425 4.0140004 4.8736405 28
CGAAA 42890 4.010366 5.448545 8
GGCGT 24405 3.9992642 4.867293 17
ACGGT 39465 3.9920065 5.2035785 15
ACTCA 1250 3.9614606 109.00122 23
TCGTA 92995 3.8774455 4.6804976 9
AAACG 41440 3.8747861 5.4821987 2
GACGT 38025 3.8463461 4.870958 28
CGTTA 92245 3.846174 4.508372 9
TTACG 92205 3.8445063 4.9952574 2
GCGAT 37950 3.83876 4.726239 19
ATCGA 61445 3.836574 4.8811545 2
TCGAT 91965 3.8344994 4.5414734 7
AGTCG 37800 3.8235867 4.625377 6
GGGGG 485260 3.800849 7.9563136 1
CGATA 60695 3.7897444 4.4017615 8
ACGAG 24770 3.7521145 4.7139144 5
CGTAG 36995 3.7421587 4.7252445 25
AGATC 59455 3.7123199 17.568819 32
CGGTG 22645 3.710852 4.488095 23
CGAGT 36310 3.6728685 4.3685226 9
AATCG 58810 3.672047 4.2263656 2
GCGTT 54205 3.6614034 4.421417 18
AAGCG 24000 3.6354764 4.302724 26
CGAAG 23955 3.62866 4.609384 24
TGGCG 22055 3.6141677 4.503034 27
ACGTT 86175 3.5930839 5.157051 15
ATACG 57320 3.5790126 5.9327855 2
TACGA 57155 3.56871 4.312487 3
AACGG 23545 3.5665538 4.4645867 24
CGATT 85490 3.5645225 4.2634234 8
ACGAA 38035 3.5564065 4.5559783 23
CGTAA 56935 3.5549734 4.392901 31
CTCGT 1030 3.531293 51.713825 8
GCGAA 23285 3.52717 4.7380857 7
TGACG 34810 3.521139 4.266371 27
TCGAA 55765 3.4819195 4.7417755 18
AACGA 37105 3.4694483 4.364664 5
TACGT 82490 3.4394374 4.7003045 3
ACGAT 55055 3.4375875 4.0999103 27
CACAA 720 3.4170296 82.13304 12
TGTCG 50530 3.413167 3.9038322 3
ACGTG 33540 3.3926744 26.228313 31
ACGTA 53665 3.3507972 4.133979 4
GACTC 650 3.337189 57.980038 21
AGCGT 32945 3.3324883 3.8895104 2
TCTTC 2355 3.3280847 55.421204 5
TCTGC 970 3.3255866 46.376972 22
GACAC 430 3.3060305 52.492035 9
CGTAT 79045 3.2957973 4.1707373 10
GCGTA 32315 3.268762 3.875814 20
ACAAC 685 3.250924 75.35142 13
CGTTG 47990 3.2415967 4.8270283 16
TTGCG 47965 3.239908 3.606922 19
ACCGT 630 3.2345064 68.47384 15
TCTCG 935 3.2055912 51.169468 7
TCGTG 47240 3.1909363 3.8161337 22
AACAC 670 3.179736 42.196793 13
TGCGG 19380 3.1758137 3.8575864 21
CGATG 31055 3.141309 3.8223817 15
TAACG 50270 3.1388164 3.727521 4
CGTCA 610 3.1318235 52.495605 27
GAGCA 20530 3.109847 73.42829 9
GAACG 20465 3.100001 3.8088524 22
AACGT 49445 3.0873039 4.2628827 15
GGCCT 370 3.0774522 25.955484 32
CTGCT 815 2.7941782 46.949093 23
CTGAC 540 2.772434 66.906845 19
CACAT 865 2.7413304 47.80178 12
GCGCA 215 2.6779284 39.552753 11
AGCCA 325 2.4987438 54.933475 10
ACACA 525 2.491584 33.155468 10
GCCTT 715 2.4513345 46.848537 1
AACCT 760 2.408568 61.026386 22
ACACT 720 2.2818012 49.311314 13
CAACA 480 2.2780197 44.457336 12
ACTCT 1060 2.2432632 25.872778 15
CTCTT 1550 2.190459 55.64554 4
CTCTG 600 2.0570638 41.92242 16
CGGCA 155 1.9305996 7.9103503 4
TTCCA 910 1.9258204 28.67945 25
ATCTC 890 1.8834946 31.920782 6
ACTTC 875 1.8517504 23.251753 23
CATAC 535 1.6955051 28.681072 12
CACAG 215 1.6530151 31.738539 12
CGGCT 195 1.6219004 26.416706 16
CCTTA 755 1.597796 28.582073 2
TCCGT 465 1.5942245 37.169174 25
TGAAC 25290 1.5790862 13.083958 20
CCAGG 125 1.5569351 17.888603 26
CAGGC 125 1.5569351 11.939531 27
CGCAA 200 1.5376885 14.648556 4
ATCAC 480 1.5212009 25.857365 28
GGCCG 75 1.5133659 6.4162216 20
CCGGG 75 1.5133659 9.628455 21
GCTCG 180 1.4971389 9.244296 11
TCGCA 290 1.4888997 9.782058 3
TACAC 465 1.4736633 14.592125 12
GACCA 190 1.4608041 9.765711 6
CTTCT 1015 1.4343975 19.772102 20
ATTCC 675 1.4284934 12.474858 24
GCCGG 70 1.4124749 9.651202 25
CTTCA 665 1.4073303 14.508428 25
CGCAG 110 1.3701029 5.9574347 25
CATCA 425 1.3468966 26.8347 27
ACGCA 175 1.3454775 9.794011 23
TCCGG 160 1.33079 6.6303596 25
AACCA 280 1.3288449 10.573804 22
CCGAG 105 1.3078254 5.9327626 12
AGCTC 240 1.2321929 9.80473 22
ACCAG 160 1.2301508 12.242514 23
CAAAC 255 1.210198 6.0281134 12
GCACT 235 1.2065222 11.412523 11
ATCCA 380 1.2042841 19.680775 23
AAACC 250 1.1864687 6.786015 18
CCGGA 95 1.1832707 5.9327626 13
CTCTA 555 1.1745387 10.459564 25
GGCTC 140 1.1644413 10.564909 10
CCGGT 140 1.1644413 7.9234786 14
CACGA 150 1.1532663 7.3242755 14
AGTCA 17985 1.1229682 17.257881 29
GCCTG 135 1.1228541 6.6071725 18
ACCGG 90 1.1209933 9.887938 13
CAGCA 145 1.1148242 18.4249 27
AATCC 350 1.109209 20.174013 22
GTCTG 16110 1.0881876 17.86352 17
CTGAA 17390 1.085817 14.527382 19
GCAGC 85 1.0587159 5.932913 11
AGCCG 85 1.0587159 5.932913 10
GCTCA 205 1.0524981 16.303606 11
CTAAC 330 1.0458256 21.153576 19
TGCTC 305 1.0456741 9.798401 10
GAACT 16660 1.0402362 10.467677 21
CGACA 135 1.0379398 6.103565 12
TCCAT 490 1.0369802 19.569506 25
TTCAC 475 1.0052359 10.415288 30
CCGAA 130 0.99949753 15.884405 19
GTCCG 120 0.99809253 13.210954 17
GCCGA 80 0.9964385 7.9105506 11
CAGCG 80 0.9964385 5.932918 7
CTGTC 290 0.9942475 5.993471 19
ACCAT 310 0.98244214 5.5424867 20
CAGAC 125 0.9610554 7.324279 12
ACTCG 185 0.94981545 13.080361 23
TGACC 185 0.94981533 8.162706 20
CCAAA 200 0.9491749 6.0587025 26
GTGGC 5755 0.94307584 6.3353877 32
GTCCA 180 0.9241446 8.166203 21
TGTCC 265 0.9085365 5.995921 20
CAACT 285 0.90321296 11.584102 19
GTCTC 255 0.8742521 8.168312 17
CACTG 170 0.8728033 11.4122305 14
TCAAC 275 0.8715213 10.573545 18
ACCTG 165 0.84713256 8.966812 15
GCTGC 100 0.8317438 5.285738 18
GGACC 65 0.80960625 9.887945 5
GCCAG 65 0.80960625 19.858116 25
ACCAA 170 0.80679864 5.274737 7
ACATC 230 0.7289088 5.0317674 13
GCGCT 85 0.70698225 5.4578953 30
CCTAG 135 0.6931085 10.650848 26
CCAAG 90 0.6919598 13.49598 26
TCTGA 16415 0.6844268 10.234594 18
TCCAA 210 0.6655253 9.094845 25
GGTCC 75 0.62380785 5.3225327 28
AGGCC 50 0.62277406 5.932918 8
TCTCA 285 0.60314155 6.4107003 25
TCTAC 285 0.60314155 6.7245064 18
CCGTA 110 0.5647551 5.716342 21
TACTC 260 0.5502344 5.719921 20
CTCGA 105 0.5390844 5.725652 24
CTACT 245 0.5184901 5.717583 19
GACCT 90 0.4620723 5.7163415 21
Produced by FastQC (version 0.10.1)