##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename filtered_Y54_Mix_GTGGCC_L005_R2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15646291 Filtered Sequences 0 Sequence length 36 %GC 23 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.82357799685561 34.0 31.0 34.0 23.0 34.0 2 31.358743423601158 34.0 33.0 34.0 25.0 34.0 3 31.473362600759504 34.0 33.0 34.0 26.0 34.0 4 34.8683535925543 37.0 37.0 37.0 33.0 37.0 5 34.411986137800966 37.0 37.0 37.0 28.0 37.0 6 34.393669081062086 37.0 37.0 37.0 30.0 37.0 7 34.54068168615808 37.0 37.0 37.0 32.0 37.0 8 34.77407239837224 37.0 37.0 37.0 33.0 37.0 9 35.46318063495048 39.0 39.0 39.0 21.0 39.0 10 35.65684078098765 39.0 39.0 39.0 27.0 39.0 11 35.826034809144225 39.0 38.0 39.0 29.0 39.0 12 35.540775062920666 39.0 39.0 39.0 25.0 39.0 13 35.53917442798424 39.0 39.0 39.0 27.0 39.0 14 36.886657419320656 41.0 40.0 41.0 18.0 41.0 15 37.435542583223075 41.0 40.0 41.0 27.0 41.0 16 36.91487119854795 41.0 40.0 41.0 27.0 41.0 17 36.70696582340185 41.0 40.0 41.0 18.0 41.0 18 36.6981239835051 41.0 40.0 41.0 25.0 41.0 19 36.45713466533378 41.0 40.0 41.0 18.0 41.0 20 37.02753687758971 41.0 40.0 41.0 25.0 41.0 21 36.60032291359019 41.0 39.0 41.0 25.0 41.0 22 36.81718057014279 41.0 39.0 41.0 27.0 41.0 23 36.95085538163645 41.0 39.0 41.0 27.0 41.0 24 36.72145577504598 41.0 39.0 41.0 24.0 41.0 25 37.34140327570285 41.0 40.0 41.0 30.0 41.0 26 37.576507301315054 41.0 40.0 41.0 32.0 41.0 27 36.500901395736534 41.0 40.0 41.0 18.0 41.0 28 36.46379771410362 41.0 40.0 41.0 18.0 41.0 29 36.33318701537636 41.0 38.0 41.0 25.0 41.0 30 35.77865303668454 41.0 39.0 41.0 15.0 41.0 31 35.941681386342616 41.0 39.0 41.0 17.0 41.0 32 36.15705389858849 41.0 39.0 41.0 16.0 41.0 33 36.555937378385714 41.0 40.0 41.0 25.0 41.0 34 36.141401243272284 41.0 39.0 41.0 19.0 41.0 35 36.328854167418974 41.0 40.0 41.0 24.0 41.0 36 35.88332602276156 40.0 38.0 41.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 383324.0 3 9856.0 4 54696.0 5 25552.0 6 28192.0 7 32823.0 8 25003.0 9 20091.0 10 20506.0 11 22363.0 12 46185.0 13 44353.0 14 34289.0 15 41827.0 16 56855.0 17 73634.0 18 81990.0 19 88998.0 20 99631.0 21 111020.0 22 116020.0 23 116488.0 24 115057.0 25 111644.0 26 106645.0 27 102857.0 28 103234.0 29 105787.0 30 116706.0 31 138844.0 32 153653.0 33 163077.0 34 158774.0 35 178053.0 36 275770.0 37 567299.0 38 1734000.0 39 9981195.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.914278959331973 56.09184458573149 19.60366517782333 13.390211277113204 2 0.30610992326814646 58.10931419249575 30.2328610687019 11.351714815534201 3 0.13204691730621204 41.06219870896877 45.76722906588847 13.038525307836544 4 0.2963907420089689 43.601076866666624 29.357731641009078 26.744800750315335 5 11.817699478268995 44.11160507846757 28.312843007237444 15.757852436025994 6 12.110174295744596 44.8630736953103 25.152348185407046 17.874403823538056 7 0.559378951002103 55.15023121454426 27.73396269775908 16.556427136694555 8 0.498643730912669 54.93791543344915 26.15000697572186 18.413433859916335 9 10.626089765407313 44.195152438449 26.86626959972895 18.31248819641474 10 1.1317587078698037 54.64732083151658 26.63829121134991 17.582629249263707 11 12.419265816086725 42.08127511361899 28.325218585228868 17.174240485065415 12 1.0717315638922382 41.980158385057834 28.315756097526485 28.632353953523438 13 10.773675808204363 42.105591666681114 30.37198986461169 16.748742660502828 14 0.6665325128769987 41.76997631768388 40.57534003011079 16.988151139328338 15 0.6050764675668059 42.290394452013416 28.275318557872296 28.829210522547477 16 10.530576464068012 42.774853500572036 29.70242792312905 16.9921421122309 17 4.17408817100276 42.2775991680125 36.30440480059561 17.24390786038913 18 9.503064989172195 42.282001345097676 30.72171115529715 17.493222510432982 19 0.7785171806858562 42.181119175340434 39.19171154889823 17.84865209507548 20 0.6580406041912285 54.02079169960391 27.519795893246684 17.801371802958187 21 11.690441521880501 42.17739343880094 28.408239697966387 17.723925341352174 22 11.983886958221802 41.71587748241012 28.775012928918308 17.525222630449765 23 12.141986802772832 42.13550229698087 28.2976221296724 17.424888770573897 24 1.0117874294897995 53.90807790178953 27.781221240717578 17.298913428003097 25 0.6108101893342566 54.44339139852931 27.70413632822248 17.241662083913955 26 0.6485641115065096 54.48904444968866 27.482203059086185 17.380188379718646 27 10.576231721974914 44.26102714968779 27.580438586593104 17.582302541744188 28 1.6060932419543867 53.51253550903884 27.078483800946568 17.802887448060208 29 12.454797475745206 41.95834769047324 27.683221069485818 17.90363376429573 30 3.722092763299737 42.02271294623039 36.17372287349755 18.081471416972313 31 11.674897245912423 41.4791143580389 28.90066947552346 17.945318920525217 32 0.8241304491336279 41.70877992783593 39.61837473892732 17.848714884103124 33 0.968073749459528 53.47315022043809 27.85244480953767 17.706331220564707 34 12.425122140824268 42.1762168121839 27.947544676838938 17.451116370152892 35 0.8582547225559406 53.92469166316391 27.80175824706616 17.415295367213986 36 0.6888591691954197 42.305663863742296 39.437227678427995 17.56824928863429 >>END_MODULE >>Per base GC content fail #Base %GC 1 24.30449023644518 2 11.657824738802347 3 13.170572225142754 4 27.0411914923243 5 27.575551914294987 6 29.984578119282652 7 17.11580608769666 8 18.912077590829 9 28.938577961822055 10 18.71438795713351 11 29.59350630115214 12 29.704085517415677 13 27.522418468707187 14 17.65468365220534 15 29.434286990114284 16 27.522718576298917 17 21.417996031391887 18 26.996287499605177 19 18.627169275761336 20 18.45941240714941 21 29.414366863232676 22 29.509109588671574 23 29.566875573346728 24 18.310700857492897 25 17.852472273248214 26 18.028752491225156 27 28.158534263719105 28 19.408980690014594 29 30.35843124004094 30 21.803564180272055 31 29.62021616643764 32 18.67284533323675 33 18.674404970024234 34 29.87623851097716 35 18.273550089769927 36 18.25710845782971 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 61838.0 1 65050.5 2 68263.0 3 68263.0 4 165521.0 5 262779.0 6 262779.0 7 478943.5 8 695108.0 9 695108.0 10 991377.5 11 1287647.0 12 1287647.0 13 1555476.5 14 1823306.0 15 1955187.0 16 2087068.0 17 2087068.0 18 2055017.0 19 2022966.0 20 2022966.0 21 1870278.5 22 1717591.0 23 1717591.0 24 1513157.0 25 1308723.0 26 1116255.0 27 923787.0 28 923787.0 29 772781.5 30 621776.0 31 621776.0 32 516637.0 33 411498.0 34 411498.0 35 343597.0 36 275696.0 37 275696.0 38 238987.5 39 202279.0 40 187199.0 41 172119.0 42 172119.0 43 180297.5 44 188476.0 45 188476.0 46 246725.5 47 304975.0 48 304975.0 49 418002.0 50 531029.0 51 406168.0 52 281307.0 53 281307.0 54 244639.5 55 207972.0 56 207972.0 57 143637.5 58 79303.0 59 79303.0 60 57275.0 61 35247.0 62 35247.0 63 26538.5 64 17830.0 65 15361.0 66 12892.0 67 12892.0 68 11396.0 69 9900.0 70 9900.0 71 8600.5 72 7301.0 73 7301.0 74 6746.5 75 6192.0 76 5872.0 77 5552.0 78 5552.0 79 4676.0 80 3800.0 81 3800.0 82 3401.0 83 3002.0 84 3002.0 85 2800.5 86 2599.0 87 2599.0 88 2490.0 89 2381.0 90 2153.5 91 1926.0 92 1926.0 93 1650.0 94 1374.0 95 1374.0 96 965.0 97 556.0 98 556.0 99 394.5 100 233.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3013941131479659 2 0.39638787237179723 3 0.4764899233946243 4 0.4876810740641344 5 0.40370590065083156 6 0.40416607360811585 7 0.4091065416078481 8 0.4527718422212651 9 0.4371451355468206 10 0.4558780096829338 11 0.44949950119168813 12 0.5648878702307147 13 0.46993885004439706 14 0.42921354332474065 15 0.46795754981164545 16 0.46670485676126056 17 0.45414596980204447 18 0.44564555267443257 19 0.44008512944058115 20 0.46324077699948185 21 0.5981289751034287 22 0.4892085926306752 23 0.4124491868392323 24 0.453046667737421 25 0.5505330304798755 26 0.41608583145999267 27 0.42512950832884294 28 0.4202337793666243 29 0.43150801682008855 30 0.4551174460452001 31 0.46310016859586717 32 0.4729491481399649 33 0.5041578224513401 34 0.5023874348240104 35 0.4959833611684712 36 0.7365962962084752 >>END_MODULE >>Sequence Length Distribution pass #Length Count 36 1.5646291E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 18.267129568379133 #Duplication Level Relative count 1 100.0 2 4.935921766505169 3 1.495344839134609 4 0.8986581526714149 5 0.6067337962446137 6 0.4733305052579875 7 0.35946326106856596 8 0.31927482194288775 9 0.23722342539462815 10++ 2.308602558663958 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT 388364 2.48214736642697 Illumina Single End PCR Primer 1 (100% over 36bp) GATCGGAAGAGCGTCGGGTAGGGAAAGAGGGTAGAT 133916 0.855896135384418 Illumina Single End PCR Primer 1 (96% over 29bp) GATCGGAAGAGCGTCGGGTAGGGAAAGAGTGTAGAT 129784 0.8294873206691604 Illumina Single End PCR Primer 1 (97% over 36bp) GATCGGAAGAGCGTCGTTTAGGGAAAGAGTGTAGAT 60260 0.3851392000826266 Illumina Single End PCR Primer 1 (97% over 36bp) GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGAT 54305 0.3470790617405748 Illumina Single End PCR Primer 1 (97% over 36bp) NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 42225 0.2698722655739945 No Hit GATCGGAAGAGCGTCGGGTAGGGAAAGGGGGTAGAT 29449 0.18821713082033306 Illumina Single End PCR Primer 1 (96% over 27bp) GATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTAGAT 27591 0.17634211200597 Illumina Single End PCR Primer 1 (97% over 36bp) GATCGGAAGAGCGTCGTATAGGGAAAGAGTGTAGAT 17352 0.1109016827055051 Illumina Single End PCR Primer 1 (97% over 36bp) >>END_MODULE >>Kmer Content fail #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position GGGGG 653265 2946.7627 15033.129 27 GAGGG 447480 232.65047 5403.342 27 GAGCG 1274960 198.02425 6275.838 9 TAGGG 1451965 134.60364 4262.976 19 GGGGT 160090 128.76276 2159.75 28 AGGGG 238770 124.13952 1491.061 26 TCGGG 513505 123.385 3819.105 14 GTCGG 492245 118.27665 3731.6794 13 CGGGT 488770 117.44167 3713.667 15 GGGCG 84235 113.511505 2351.2546 9 GTAGG 1181270 109.50901 3459.8257 18 GCGTC 1428795 102.56027 3260.1855 11 CGTCG 1317315 94.55812 2988.5042 12 GGGAA 1557400 93.3265 2812.8838 21 GGAAG 1540825 92.33325 2802.3718 5 GAGTG 987695 91.56374 2881.3364 27 AGGGA 1476220 88.46182 2789.1074 20 GGGTA 912720 84.61321 1429.478 16 GGTAG 895290 82.997375 1382.5234 17 GGGGA 156890 81.56908 866.10535 21 GGGTG 90340 72.661804 1198.5425 27 CGGGG 51025 68.75913 1616.0531 15 GGGAG 100850 52.433178 554.63007 17 TGGGG 65140 52.393074 337.39658 19 GGCGG 37430 50.43908 1121.1244 13 GCGGG 35095 47.29254 1088.6838 14 ATCGG 1699085 47.055157 1460.4366 2 TCGGA 1672325 46.31406 1455.0382 3 GGGGC 32945 44.395283 903.05566 11 GATCG 1571145 43.51194 1350.887 1 GGAGG 82465 42.874588 607.6632 18 AGGGT 419555 38.89462 1005.19055 28 AGCGT 1384120 38.332394 1216.3529 10 GTCGT 869105 37.235764 1174.924 13 GGTGG 44335 35.659298 305.2407 12 GTGGG 43315 34.8389 270.41254 18 TGTAG 1909340 31.561256 605.6175 30 GTGTA 1886790 31.188501 609.31213 29 TCGTG 723320 30.989777 978.3364 14 CGTGT 721540 30.913515 973.7383 15 CGGAA 1675940 30.002323 945.2088 4 AGAGC 1419675 25.41472 804.45703 8 AGAGG 384105 23.017324 629.9069 26 AAGAG 2852485 19.701658 318.59097 7 AGTGT 1140620 18.854366 593.12 28 GTAGA 1613145 17.23648 534.12085 31 AAGGG 286665 17.178274 235.51433 25 GGCGT 60350 14.500898 403.15765 10 GGAAA 1819450 12.5666485 344.11993 22 GGTCG 49970 12.006792 339.5858 12 GGGTC 49390 11.867429 356.71054 11 AGAGT 1059020 11.315645 352.35062 26 GAAGA 1473075 10.174293 313.451 6 GAAAG 1459980 10.083849 311.09946 23 AGGGC 60360 9.374995 266.8859 8 GGTGT 60990 8.746918 216.21916 28 GAAGG 130155 7.7994814 153.89655 6 CCCCC 631235 6.7749257 8.16771 26 GCGGC 12320 4.959634 126.47645 11 CGGCG 12195 4.9093127 121.38281 12 TTAGG 288280 4.7652473 149.99155 18 TTGGG 33040 4.738452 68.65962 19 CGGGA 24170 3.7540371 60.687187 4 TAGAT 1748220 3.3307464 104.418495 32 TGTGG 22285 3.196017 50.140945 30 GTGTG 22180 3.1809585 52.80915 29 GGGTT 21595 3.0970602 22.541515 16 CTCTC 797810 3.0504963 3.6578372 31 GAGCT 106070 2.9375465 92.068436 9 GTTGG 19785 2.8374782 47.251873 18 GTGGT 19665 2.8202682 32.550556 31 CCGGG 6980 2.8099222 57.541542 14 GCTTC 216415 2.7699177 86.21493 11 GCCGG 6405 2.5784457 56.59315 13 GCGTG 10085 2.4232242 28.395256 11 CGTGG 10000 2.4028003 31.231821 15 GGTTG 15910 2.2817426 22.23672 17 AAGCG 127040 2.274243 69.06836 9 CGCCG 18910 2.274165 46.063778 12 GGAGA 35275 2.1138387 30.451002 31 TGGGT 13460 1.9303743 31.17994 32 CTTTT 1411545 1.9227523 12.037563 15 ACGGG 12155 1.8878906 35.500786 3 AGCGG 11965 1.8583802 42.145004 10 GATGG 19805 1.836012 30.970554 1 TTTTA 3467330 1.8222449 5.7541757 16 CGTTT 234985 1.7951404 48.274662 15 TCTTT 1308850 1.782865 11.981383 14 TCGTT 231585 1.7691665 48.28011 14 GCGCC 14425 1.7347873 47.23889 11 ATGGG 18515 1.7164233 33.2683 2 TTCTT 1232365 1.6786802 9.390361 13 CGTCT 130770 1.6737386 43.959682 12 GAGAG 27625 1.6554158 15.572217 27 GACGG 10655 1.6549135 33.01064 2 CTTCT 711810 1.6244782 14.284375 12 TGGTG 11290 1.6191623 26.827946 14 GTGGA 17395 1.6125942 13.478577 31 AAGTG 148490 1.5866181 47.886074 27 GGACG 10140 1.5749246 32.663105 1 GGGCC 3680 1.4814489 14.439715 11 TGGGA 15900 1.474001 20.59427 20 CGGGC 3650 1.469372 23.94258 15 CAGGG 9200 1.4289259 32.83786 19 TTTAG 479080 1.4120474 29.868774 17 GCGCG 3500 1.4089868 10.247118 9 GGCCG 3230 1.3002934 14.310665 12 CTTCG 98985 1.2669191 30.416601 12 GGGCA 8025 1.246427 12.580191 21 AAAGG 177880 1.2285886 24.063936 24 AAAGA 1467750 1.1684388 35.06682 24 GCAGG 7360 1.1431407 27.141567 18 GAGGT 11750 1.0892774 16.341848 27 GAGTT 65160 1.07709 31.973417 27 AGCTT 215210 1.062735 33.233784 10 GTTTA 345680 1.0188624 18.52111 16 TGGAG 10675 0.9896202 10.424054 17 TATGG 58840 0.97262096 28.885157 19 TCGCG 13465 0.96653044 9.1239195 14 TAGTG 58325 0.96410805 19.91514 19 CGCGG 2275 0.9158414 5.472603 13 GTCTT 113535 0.86733747 25.143095 13 TATCG 175285 0.8655801 18.55444 1 GGCGC 2140 0.8614948 6.251225 10 CGACG 18350 0.85143095 5.594694 12 CGCGT 11680 0.8384014 8.618629 15 TTCGT 101100 0.7723416 15.7732315 13 GCCGT 10725 0.7698506 17.79414 13 GGCAG 4845 0.7525158 8.981067 17 GGGCT 2985 0.7172358 7.3471127 9 GAGCA 39925 0.71472883 20.769228 9 GGGAC 4595 0.7136863 10.392331 21 GCGGT 2950 0.7088261 10.218906 14 CGGTG 2935 0.7052219 11.716735 15 TGGAA 65575 0.70066994 18.87051 21 AGCGC 14770 0.6853208 18.346992 10 AGGAA 97075 0.6704814 10.251033 21 ATGGA 59515 0.63591874 18.657377 20 CCGTG 8605 0.61767507 14.999953 14 GTGAA 57105 0.61016786 12.947499 21 TCGTA 117765 0.5815389 5.938831 14 GTAAG 54305 0.5802498 11.692607 18 TTCGG 13530 0.5796766 14.389423 13 GAACG 32380 0.5796599 7.866915 9 CGGAG 3695 0.5739001 7.452376 4 ATCGT 115670 0.57119346 5.1749926 2 TCGGT 13330 0.57110786 14.926238 14 CATCG 67140 0.5554757 9.843295 12 TAAGG 51910 0.5546592 15.173367 19 CGTCA 66875 0.5532833 9.743935 12 GGGAT 5905 0.54741985 5.7576756 21 CGGTT 12585 0.5391893 14.432503 15 AGTGG 5735 0.53166014 5.4086323 28 AGTGA 48245 0.5154986 12.902034 20 ATAGG 47965 0.5125068 13.645368 18 GAGGC 3255 0.5055601 5.247785 11 AAAGC 244720 0.5049406 15.088013 8 GAAAA 618235 0.49216136 5.8272395 23 TGAAG 45620 0.48745042 9.771819 5 TAGGA 45005 0.48087916 12.943398 19 AATCG 143400 0.45773682 6.01904 1 TGGGC 1900 0.45653206 6.426928 20 GTAGT 27550 0.45539957 10.630283 18 AAAAG 569640 0.45347604 6.037894 7 GTCGC 6095 0.43750492 9.275914 13 GACCG 9020 0.41852355 5.7952785 1 GCATC 50515 0.4179305 11.597437 11 AAGGA 59860 0.41344342 10.161903 20 AGTTT 139765 0.41194546 11.749363 28 TAGAG 38475 0.411106 10.795578 19 GGTAA 38345 0.4097169 5.3568783 21 TGGTT 15995 0.40902606 6.860291 32 TAGCG 14700 0.4071079 11.1372385 9 TCTTG 53050 0.40526927 11.853837 14 CTTGT 52985 0.4047727 11.730041 15 GTATG 24270 0.40118134 11.440461 18 CGTAT 81105 0.40050703 5.8951893 15 AGAGA 57960 0.4003204 7.2126727 20 CTCGG 5475 0.3930007 5.827728 32 GTTAG 21415 0.35398847 5.544298 17 TTAGA 183365 0.34935093 9.380715 31 GGTTA 21045 0.34787232 5.3970923 16 GCGAC 7415 0.34405237 5.542733 11 GAGAA 49620 0.34271735 7.2338767 21 AAGCT 106015 0.33840287 10.0685005 9 TAGGT 20370 0.33671463 8.024832 19 ATCTG 65065 0.3212994 9.436287 2 TCTGA 64065 0.3163613 9.377927 3 GATCT 63880 0.31544775 6.451212 1 TCTGG 7350 0.31490192 7.5144944 14 CGTGC 4175 0.29968545 7.3447704 15 GTCTG 6900 0.29562223 7.6812124 13 ACCGG 6325 0.29347688 5.8842845 2 CGTGA 10575 0.29286844 7.1109366 15 CCGGA 6305 0.2925489 5.8482165 3 CTGGT 6770 0.29005253 7.322422 15 AAAGT 221240 0.27246666 7.7604146 26 GGAAT 25480 0.27225417 7.0645404 5 GTCAT 50615 0.24994345 5.8347945 13 GGTAT 14795 0.24456027 5.1524367 17 AGGTA 20960 0.22395794 5.3788424 20 CTGAA 66795 0.21321152 6.111513 4 >>END_MODULE