FastQCFastQC Report
Tue 3 Jun 2014
filtered_Y54_Mix_GTGGCC_L005_R2_tr.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename filtered_Y54_Mix_GTGGCC_L005_R2_tr.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 13412322
Filtered Sequences 0
Sequence length 19-36
%GC 19

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 245 376456.22 4692382.0 27
GAGGG 2170 29583.07 705554.8 27
GGGCG 415 14274.2295 361888.34 9
AGGGG 585 7975.1597 100313.83 20
GGGGA 465 6339.2295 89419.836 21
GGGTG 295 6254.451 88599.63 27
GGGGT 280 5936.428 27134.617 21
TGGGG 260 5512.398 61012.52 19
CGGGG 155 5331.338 65712.13 15
GTGGG 250 5300.3823 57587.336 18
GAGCG 16460 5023.073 127993.13 9
GCGGG 120 4127.4873 38493.0 19
GGTGG 185 3922.283 23701.375 17
GGGAG 255 3476.3516 23948.842 17
GGAGG 240 3271.8604 34850.93 18
GGAAG 26975 3262.7224 58014.203 5
TAGGG 16630 3128.2007 92776.234 19
GGGGC 85 2923.6372 27510.217 20
GTAGG 13660 2569.5264 74746.17 18
TCGGG 4585 2176.0193 60524.06 14
CGGGT 4515 2142.7974 59388.96 15
GGCGG 60 2063.7437 27478.016 18
GTCGG 4330 2054.9976 57967.82 13
GAGTG 10485 1972.2902 59107.215 27
GGGAA 16290 1970.3335 57257.2 21
AGGGA 15940 1927.9998 57134.91 20
GGGTA 6335 1191.6508 23434.531 16
GGTAG 5715 1075.0251 22560.598 17
GCGCG 760 585.1595 1601.292 21
AGGGT 2925 550.2097 9323.546 28
CCGGG 475 365.72476 6893.313 19
AAGGG 2570 310.85065 5124.4087 19
GGGTT 1050 307.16757 6973.3076 21
GGGTC 640 303.74097 5070.274 11
CGGGC 365 281.03055 7020.2896 20
AGGGC 910 277.70334 4637.4873 20
AGAGG 2130 257.6311 6290.685 26
TTGGG 880 257.43567 6220.3804 19
GGCGT 520 246.7895 5371.5312 10
GGTCG 510 242.0436 4842.8066 12
CGTCG 22745 241.63814 4622.499 12
GATCG 56185 236.58049 2174.9116 1
CGGGA 765 233.45389 4002.8838 20
GGGCC 270 207.88564 6158.8164 21
ACGGG 650 198.35951 4683.7114 19
ATCGG 47030 198.03116 2315.0789 2
GCGTC 18485 196.38077 4997.292 11
GGGCT 410 194.58406 4707.4053 21
GGGCA 635 193.78201 4882.095 21
GCCGG 250 192.48668 4428.6772 18
GTTGG 655 191.61406 4954.5337 18
TGGGT 640 187.22595 4071.2217 20
TGGGC 390 185.09216 4479.1426 20
CGGCG 240 184.78722 736.55585 12
GGGAC 590 180.04941 4100.9595 21
GAAGG 1450 175.38266 3208.024 18
TCGGA 40325 169.79813 2307.9255 3
CAGGG 530 161.7393 4439.768 19
CGCGG 210 161.68881 1230.1882 18
TGGGA 855 160.83052 3941.7866 20
ATGGG 820 154.24683 3368.2134 19
GGGAT 805 151.42523 3912.12 21
CTGGG 280 132.88667 3186.7788 19
GGCCG 170 130.89095 985.84735 22
GATGG 635 119.447235 2314.216 18
GACGG 380 115.96403 2632.9602 18
CGCCG 6455 111.253365 184.11343 12
GCGGC 140 107.79254 736.6228 11
CCGCG 6020 103.75605 179.10576 19
GCAGG 335 102.231445 2925.5112 18
GGTGT 340 99.46378 1271.0945 28
GCTGG 205 97.29203 2502.3508 18
TCGCG 9140 97.101456 131.31204 26
CGGAA 35625 96.45609 1507.584 4
GGACG 315 96.128075 777.2777 5
CGCGA 14030 95.841484 140.96297 20
CGACG 13610 92.9724 119.61802 31
GTCGT 13885 90.92601 2075.217 13
CGCGC 4850 83.590836 149.77478 28
AGCGT 18020 75.877556 1968.7338 10
GTGTG 255 74.59784 652.8903 9
CGTGG 150 71.18929 605.3508 12
GTGCG 150 71.18929 680.91364 9
GCGTG 150 71.189285 605.40576 11
TCGTG 10675 69.9053 1808.8969 14
CGTGT 10635 69.643364 1817.1862 15
ACGCG 10160 69.404816 101.14652 14
AGCGG 220 67.13707 341.1505 17
GCGAG 220 67.13707 535.129 9
CGCGT 6205 65.92063 99.03732 27
GGTTG 225 65.821625 931.3922 5
GGCGC 85 65.44547 246.75362 23
GGCGA 210 64.08538 293.0555 22
CGGAG 205 62.55954 340.1025 14
GTGTA 22730 58.995735 971.8312 29
GTGGT 200 58.508106 419.2729 13
TGTAG 22245 57.736923 943.4673 30
TGTGG 195 57.045406 467.1909 17
CGGTG 120 56.951424 379.3784 19
CGAGG 185 56.45617 243.0223 2
GCGGA 185 56.45617 341.30966 18
AGAGC 20395 55.220272 1296.1971 8
TGGTG 180 52.657295 279.45343 14
TGGCG 110 52.205482 302.6283 9
GGCTG 110 52.205475 831.0604 5
GCGGT 110 52.205475 303.67065 20
GGCAG 160 48.826958 728.6979 5
GAGAG 390 47.17189 443.47855 9
GGAGC 135 41.197746 243.24873 8
GTCCG 3785 40.211052 61.107273 18
AAGAG 37190 39.909843 526.1946 7
GGATG 205 38.561707 419.22476 5
AGGCG 125 38.14606 195.37035 22
TGCGG 80 37.96762 303.1738 17
GTGAG 190 35.740116 419.81363 9
AGTGT 13355 34.662918 967.35925 28
GGAGA 275 33.26223 193.58765 22
GAGGT 160 30.096941 151.16934 27
GCCCG 1695 29.213703 61.169384 29
GGTGA 150 28.215881 150.27377 18
GCCGC 1585 27.31783 61.477547 31
TGGAG 145 27.275352 152.15105 30
GAAGA 24975 26.801512 515.4906 6
GGAGT 140 26.334824 179.68565 15
AGGTG 140 26.334824 150.20372 17
GGTGC 55 26.102741 230.84561 31
GCCGA 3680 25.13875 44.60134 13
GAACG 9205 24.922907 44.990314 18
GAGGA 205 24.79548 116.08854 20
GTGGA 130 24.453764 121.38272 29
GGAAA 22575 24.22599 548.07446 22
GACCG 3545 24.216541 43.97278 28
GTGGC 50 23.729763 226.82547 16
GTAGA 14165 23.640362 674.8558 31
GCGAA 8525 23.081774 55.211803 16
AGGAG 190 22.981176 154.27744 12
GAGGC 75 22.887636 195.28378 21
GACGC 3330 22.747837 44.302967 31
GCGCC 1305 22.491966 109.928444 11
GCTCG 2100 22.309963 39.108067 22
GACGA 7975 21.59263 34.607937 18
AGAGT 12675 21.153658 550.8012 26
GCGAT 4790 20.169449 47.15449 21
GCGAC 2935 20.049519 46.284935 29
AGTGG 105 19.751118 121.085014 28
GTCGA 4460 18.779905 42.914764 13
GCCGT 1660 17.635494 76.13078 13
GAAAG 16095 17.272087 452.426 23
GCGCT 1600 16.998066 49.082275 16
GCGTA 3940 16.590319 48.229347 29
GTACG 3915 16.485052 26.2382 18
GTCGC 1535 16.307522 54.137444 13
GCGTT 2435 15.94561 53.253185 11
GCGCA 2310 15.78003 46.284935 29
GACGT 3560 14.990238 33.52716 13
TGAGG 75 14.107941 60.146667 19
GCACG 1905 13.013403 32.74372 18
TTAGG 4530 11.75762 278.67712 18
GTTCG 1790 11.721827 34.527653 18
GGTTT 2415 9.748146 110.473976 22
CCCGG 550 9.479373 88.12066 18
CCGGC 540 9.30702 96.44155 19
CGGCC 535 9.220844 71.68193 20
GAGCT 2050 8.632019 162.44263 9
GGCCC 480 8.272907 77.23851 22
ACCGG 1205 8.231575 73.12764 18
CTCGG 760 8.074082 50.954212 19
AAGCG 2925 7.9195547 142.4344 9
GTGTT 1935 7.8106265 61.21069 29
TCGGC 735 7.808488 49.255733 19
CGGCT 735 7.808487 71.37536 20
TCGGT 1180 7.7272377 52.346962 19
TTCGG 1165 7.62901 53.360916 18
TTTGG 1885 7.6088014 65.138405 18
CCCCG 19500 7.5232887 10.91756 19
CGGTC 655 6.958583 49.282978 20
CGGTT 1060 6.941416 50.286327 21
CCGGA 1015 6.9336505 53.51447 19
AACGG 2460 6.6605477 50.181507 18
TGTGT 1560 6.2969394 53.2656 28
TCCGG 585 6.2149186 54.31758 18
CGGAC 895 6.113908 44.807034 21
TTGGT 1495 6.034567 55.294502 3
ACGGC 870 5.9431286 46.96167 19
CCGGT 545 5.789967 45.858788 19
AAGTG 3460 5.7744894 104.07839 27
CCGCC 14875 5.738919 7.381628 32
TGGTT 1390 5.6107345 47.57425 4
GGCTC 525 5.5774903 39.090736 21
TTGCG 835 5.468003 55.257374 14
CCCGC 14055 5.4225545 7.48957 30
ACGGA 1970 5.3338532 40.971043 3
GAGTT 2055 5.3337545 45.471478 27
ACGGT 1265 5.326587 39.740196 20
CCGTG 500 5.3118963 72.7311 14
CTGTG 810 5.30429 50.044407 14
TACGG 1250 5.2634263 39.020912 18
CGGCA 770 5.2600102 41.52397 20
GGCTT 795 5.206062 55.5491 22
CGGTA 1230 5.179211 41.09177 21
GGTCT 790 5.1733203 49.260532 22
GAGCA 1880 5.0901747 57.405384 9
AGCGC 745 5.0892305 56.62596 10
CGGAT 1190 5.0107813 39.066628 20
CCCGA 32605 4.985816 6.849779 21
TTGTG 1230 4.9648943 37.27392 14
GGACC 715 4.884295 41.546883 22
GTGTC 745 4.878637 45.426075 29
CCGAG 710 4.85014 34.80311 14
CGCCC 12565 4.847698 7.393713 17
TATGG 1860 4.827632 65.562805 19
CCGTC 20235 4.812148 7.0175276 31
GGCTA 1125 4.7370834 46.50146 22
CGTGC 445 4.7275877 47.358444 15
TGCGT 720 4.714925 44.83018 15
CGAGC 690 4.7135158 34.078903 28
TAGTG 1815 4.7108345 46.059944 19
CCCCC 543120 4.690564 5.7706423 18
TTGGC 715 4.6821823 44.85277 3
AAAGG 4360 4.6788626 36.864056 18
GGATC 1110 4.6739225 34.185764 5
GGCCT 435 4.6213493 45.90947 22
CACGG 675 4.611048 39.29246 18
CCTCG 19385 4.610007 6.20073 20
CTCCG 19335 4.5981164 5.8931394 19
TGCGC 430 4.568231 43.975697 15
GGCAA 1675 4.5351286 51.134712 22
GGTTC 690 4.518469 55.549103 22
ATAGG 2695 4.4977603 51.997562 18
TAGCG 1055 4.442332 40.275032 9
TGTTG 1085 4.379602 50.926086 17
ACGTG 1040 4.379171 38.212505 14
TAAGG 2620 4.3725905 49.361515 19
CTGCG 410 4.3557544 32.136993 14
CGACC 28390 4.341276 5.8986773 27
TTGGA 1660 4.3085318 50.02478 3
CCGAC 28170 4.3076353 5.5054326 27
GGACA 1590 4.3049884 43.334503 22
AGGAA 4010 4.3032656 42.057102 20
TGGCC 405 4.302636 55.837864 4
ATTGG 1655 4.295554 55.393963 2
CGTTG 655 4.2892714 49.152813 17
GGTTA 1650 4.2825766 42.787544 22
GAGCC 625 4.269489 34.82515 16
CTCGC 17945 4.267556 5.726131 26
CGAGA 1575 4.264375 25.863417 15
GGAAC 1550 4.1966867 43.334503 22
AGGTT 1610 4.1787567 36.540154 21
CGTCC 17540 4.1712413 5.7944894 32
TGGCT 635 4.158302 41.719353 4
ACGAG 1535 4.1560736 29.592052 27
CGTGA 985 4.14758 42.233677 15
TGCGA 985 4.14758 30.837288 15
GGCCA 605 4.132865 38.266865 22
TCCGT 28030 4.1088614 5.7387376 30
CCGAA 67685 4.102263 5.0348663 32
TACCG 43500 4.10019 4.938826 27
GTGCT 625 4.0928164 32.74903 29
ACCGA 67455 4.0883226 4.854738 26
CCGAT 43095 4.062016 5.308977 28
GTGAA 2415 4.0304604 34.298443 16
AAGGA 3750 4.024251 41.542118 20
TCTCG 27365 4.0113807 6.0952806 25
GGTAT 1545 4.0100493 43.202965 22
TAGGT 1535 3.9840941 39.68908 3
GGTCC 375 3.9839222 35.707367 22
GGCAC 580 3.9620857 40.453545 22
TGGTC 605 3.9618459 42.762333 4
GGATT 1525 3.9581392 40.29507 22
GTTTG 980 3.9557695 39.216682 1
TAGGA 2365 3.947014 47.226963 19
TCGAG 935 3.937043 26.1454 14
GGTAC 935 3.937043 43.805725 22
CTCGA 41650 3.9258144 5.3107533 26
TCCCG 16495 3.922727 5.160133 27
GGTAA 2350 3.9219801 35.259037 22
CGAAA 163170 3.9196773 4.8592906 32
TCGCC 16405 3.9013236 4.7396774 27
CCTGG 365 3.8776844 35.645912 18
TGTCG 590 3.8636189 50.19862 17
ACCCG 25190 3.851946 5.647833 7
TGGAA 2300 3.8385336 43.78727 21
CGAGT 910 3.8317745 30.166912 15
CTTGG 585 3.8308764 36.620235 18
CAGCG 560 3.8254623 30.742931 27
AGCGA 1395 3.777018 25.707205 28
TCCGA 40010 3.7712324 4.630569 31
CAGTG 895 3.7686133 32.178955 14
TGGAC 885 3.7265058 38.226868 4
ATGGA 2230 3.7217088 45.384327 20
TTCCG 25360 3.7174716 4.57501 22
AGTGA 2225 3.7133641 33.637794 20
CGAAC 61105 3.7034607 4.683221 21
TGTGC 565 3.699906 34.40456 15
CGCTC 15530 3.693237 5.243054 28
GGTCA 875 3.6843984 38.414253 22
CAGAG 1360 3.682254 31.767939 27
GGATA 2200 3.6716409 35.793262 22
CTGGT 560 3.6671636 39.637333 3
GGACT 870 3.6633446 37.066383 22
GGCAT 870 3.6633444 33.69671 22
AGAGA 3405 3.6540198 28.839155 20
TCCGC 15345 3.649242 4.619079 15
TAGGC 865 3.642291 39.572105 3
GGAAT 2175 3.6299176 35.33505 5
CCGCT 15215 3.6183262 4.8582883 31
GTGAC 855 3.6001835 28.845242 16
TTTCG 39815 3.5975583 4.74356 25
ATCCG 38135 3.5945 4.5921516 29
AACGA 149445 3.5899746 4.384345 26
GAGAA 3335 3.5789006 27.983877 21
AACCG 59040 3.578305 4.345959 17
CTGGC 335 3.5589707 37.228954 3
CTTCG 24260 3.5562248 7.1517715 12
ACCGC 22950 3.5094151 4.295605 26
ATGTG 1350 3.5039265 33.05851 14
ATGCG 830 3.494915 28.826979 14
AAGGC 1285 3.4791887 34.070656 3
GAGTC 825 3.4738615 35.322906 29
TCGCT 23570 3.4550788 4.4294276 27
TTGAG 1325 3.439039 33.471745 14
TAGAG 2055 3.4296463 33.352375 19
GTTGT 845 3.410842 32.782593 13
ACGAA 141320 3.3947954 4.171348 31
CGATA 90740 3.3899436 4.2024145 28
TGTGA 1305 3.3871288 31.404697 15
GAGAC 1245 3.370887 28.391132 29
AGTGC 800 3.3685927 32.525787 28
TCGTC 22900 3.356865 4.6349916 29
AAACG 139600 3.3534777 4.057152 25
TGCCG 315 3.3464944 42.41582 17
CGATC 35420 3.3385916 4.238987 30
TGGAT 1285 3.335219 38.444965 4
TCGAC 35345 3.3315225 4.3121505 26
CCCGT 13920 3.310358 4.511146 32
TCTGG 505 3.3069959 32.33258 2
AGGCT 785 3.3054316 36.214928 4
ACTGG 785 3.3054316 30.274845 18
TCGAA 88415 3.3030841 4.300902 32
CGTCT 22515 3.3004286 8.18013 12
CGCCT 13855 3.2949002 4.3955936 31
CGCTG 310 3.2933755 32.236023 17
CGTTT 36445 3.2930553 9.1347065 15
AGGCA 1215 3.289661 32.04985 20
GTGCA 780 3.284378 31.528519 16
AGGCC 480 3.2789674 32.64017 4
AAGGT 1960 3.2710981 27.912926 3
TATCG 55885 3.246928 5.033991 1
GTGCC 305 3.2402568 32.157356 16
ACTCG 34285 3.2316093 4.151183 29
ATGGC 765 3.221217 34.87711 3
ACGAC 53105 3.2185955 4.1693287 27
ACAGG 1185 3.2084346 32.44494 18
CAAGG 1180 3.194897 37.63613 18
CCGTA 33780 3.1840098 4.3402033 31
CACCG 20795 3.1798818 3.900945 32
ACGCC 20675 3.161532 4.3994303 27
ATCGC 33345 3.1430078 3.5753462 27
ATCGA 84115 3.142441 3.7009385 31
TGAGC 745 3.137002 33.51879 15
GTGAT 1205 3.1275787 27.290552 16
CGTAC 33175 3.1269841 4.0560093 30
ACCGT 33125 3.1222708 3.96452 30
CGAAG 1150 3.1136708 27.673439 17
TAACG 83250 3.1101255 3.9945097 25
TTCGT 34315 3.1005955 5.395859 13
TGGTA 1190 3.088646 31.004002 4
AGGAC 1140 3.0865953 31.911106 4
AGGTA 1840 3.070827 33.492176 4
CTCGT 20940 3.0695527 4.081056 28
CTACG 32560 3.069016 3.6376417 32
GAGAT 1825 3.0457928 26.38506 29
TCGTT 33525 3.0292132 8.933564 14
TGAGT 1165 3.0237586 26.314177 28
AATGG 1810 3.020759 32.69483 2
ACGTC 32040 3.0200021 3.8448055 20
TTCGC 20600 3.0197127 3.98847 22
TGAAG 1800 3.0040698 29.31828 17
TGACG 710 2.9896262 26.898424 17
AGGTC 705 2.9685724 29.50846 4
CGTAG 700 2.9475188 33.567757 12
AGGAT 1765 2.9456573 30.302444 4
CAGGT 695 2.926465 28.963194 20
TCAGG 695 2.926465 31.620394 18
ATGGT 1125 2.9199388 28.526527 3
TGGCA 690 2.9054112 29.636757 20
AGCCG 425 2.9032526 30.546555 17
CTGGA 685 2.8843577 29.620367 19
CGCAG 420 2.8690968 34.910347 17
CTAGG 675 2.84225 26.2382 18
CAGGC 410 2.8007846 32.763954 19
GTAAG 1635 2.7286966 35.465004 18
CATGG 640 2.6948743 34.984264 18
TGAGA 1595 2.6619396 24.440325 28
CAGGA 950 2.5721629 24.240528 19
CCAGG 375 2.5616937 26.194977 18
GCTGT 390 2.5539176 33.370327 13
ATGAG 1515 2.5284252 23.873652 27
AGAAG 2330 2.5004017 26.906784 17
CGATG 585 2.4632835 17.02947 30
GTTGC 375 2.45569 34.413147 13
GTTGA 945 2.4527485 25.626017 13
GCTGC 230 2.4434724 23.76394 18
AGTCG 580 2.4422297 27.570885 17
AGACG 890 2.4097106 24.214258 17
GAGTA 1435 2.3949113 20.731121 29
CTGAG 565 2.3790686 22.793425 14
GTATG 915 2.3748834 31.517057 18
GTCTG 360 2.357462 18.773743 1
AGTTG 880 2.284041 32.33137 17
AGCAG 840 2.2743335 29.835426 17
GCAGC 325 2.2201343 22.9206 18
GATGT 850 2.2061758 23.559402 13
GTAGT 835 2.1672435 30.272963 18
GTTAG 820 2.128311 19.015825 1
AGCTG 505 2.1264243 22.1912 17
GATTG 810 2.102356 29.350513 1
GCAGA 775 2.0983434 15.090759 13
TGCAG 495 2.0843167 26.225964 17
AGATG 1245 2.0778148 20.789326 17
GAAGC 745 2.017117 16.384253 13
TGATG 750 1.9466258 24.45578 17
GTAGC 460 1.9369409 22.798468 13
GTCAG 460 1.9369409 24.813978 1
GCAGT 460 1.9369409 19.510456 18
GATGC 450 1.8948334 20.786839 13
GACAG 690 1.8682024 20.332163 18
GAAGT 1110 1.8525096 19.66697 13
AGATC 49510 1.8496375 10.477339 32
GATAG 1105 1.844165 26.84695 1
TGCTG 280 1.8335816 18.824482 17
GCCAG 255 1.7419516 22.920603 18
AGTAG 1020 1.7023062 23.987684 17
GCAAG 595 1.6109861 20.764761 18
GCTTG 245 1.604384 13.601801 18
GCTGA 375 1.5790279 16.093037 13
GACTG 355 1.4948131 17.492132 18
GAATG 890 1.4853456 19.404232 1
GATGA 850 1.4185885 12.532746 18
GCTAG 325 1.3684908 16.095552 1
GCATG 305 1.284276 18.83768 18
GCCTG 120 1.274855 13.579394 18
GCTTC 7925 1.1617098 11.125987 11
TAGAT 39265 0.9041947 12.649097 32