FastQCFastQC Report
Mon 13 Apr 2015
2112_lane1_ACAGTG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_ACAGTG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC321112620.069537500000003TruSeq Adapter, Index 5 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT1972191.23261875TruSeq Adapter, Index 5 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC926020.5787625000000001TruSeq Adapter, Index 5 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC632310.39519374999999995TruSeq Adapter, Index 5 (98% over 50bp)
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC587190.36699375No Hit
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC382500.23906249999999998TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCGATCTCGTATGC363780.2273625TruSeq Adapter, Index 5 (98% over 50bp)
AACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAA309480.19342499999999999No Hit
GAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCT300670.18791875TruSeq Adapter, Index 5 (100% over 50bp)
GTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGT212070.13254375No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGACTCGTATGCC209420.1308875TruSeq Adapter, Index 5 (97% over 40bp)
CGTAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT209400.130875TruSeq Adapter, Index 5 (98% over 50bp)
GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG202900.1268125No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGAGATCTCGTATGC186940.1168375TruSeq Adapter, Index 5 (98% over 50bp)
TAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTA163020.1018875No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGGGATCTCGTATGC162310.10144375TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA206250.085.349791
CCGGAAG212850.084.903093
ACCGGAA223250.081.079392
GAGCACA5157100.077.039179
AGAGCAC5185650.076.654418
GAAGAGC5236000.076.041576
AAGAGCA5247750.075.831497
TCGGAAG4703100.075.114453
GGAAGAG5260450.074.768985
CGGAAGA5152900.074.270454
ATCGGAA4792050.073.727592
GATCGGA4813600.072.3673861
ATTGGAA145000.070.444562
TTGGAAG145450.070.386633
GATTGGA148050.068.741551
TGGAAGA162650.066.7650154
CATCGGA67900.066.6854861
ACGGAAG112400.065.934833
TCCGAAG64150.060.9480443
CGAAGAG78850.059.0317965
Produced by FastQC (version 0.11.2)