FastQCFastQC Report
Mon 13 Apr 2015
2112_lane1_ATCACG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_ATCACG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC15481379.67585625TruSeq Adapter, Index 1 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT873700.5460625TruSeq Adapter, Index 1 (100% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC254940.1593375TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGATCTCGTATGC176480.11030000000000001TruSeq Adapter, Index 1 (98% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC176320.11019999999999999TruSeq Adapter, Index 1 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC172390.10774375TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA114250.077.945431
CCGGAAG119900.077.341333
GAGCACA2712000.072.9027949
ACCGGAA127400.072.829882
AGAGCAC2739550.072.22348
AAGAGCA2790200.070.982137
GAAGAGC2795200.070.8891456
TCCGAAG58950.068.660483
GGAAGAG2807500.068.638555
CGGAAGA2775650.068.067414
CGAAGAG71550.067.9753955
TCGGAAG2578900.067.778753
ATCGGAA2640800.066.219142
GATCGGA2667250.064.453441
CCGAAGA77750.062.0770074
AATCATA1604300.061.144735
ATCATAA1717850.060.0362136
CATAAAC1727400.059.124148
TCATAAA1733450.059.0794537
TAAATCA1563800.058.1783333
Produced by FastQC (version 0.11.2)