FastQCFastQC Report
Mon 13 Apr 2015
2112_lane1_CAGATC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_CAGATC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC232333514.520843750000001TruSeq Adapter, Index 7 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT1268590.79286875TruSeq Adapter, Index 7 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC483300.3020625TruSeq Adapter, Index 7 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC349560.218475TruSeq Adapter, Index 7 (98% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC234110.14631875TruSeq Adapter, Index 7 (98% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCT233750.14609375TruSeq Adapter, Index 7 (100% over 50bp)
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC204830.12801875000000001No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA149100.083.505911
CCGGAAG153700.083.271523
ACCGGAA161000.079.588612
GAGCACA3939000.071.038449
AGAGCAC3989250.070.191248
TCCGAAG49800.069.068813
AAGAGCA4065000.068.9603657
GAAGAGC4085350.068.64176
GGAAGAG4124250.066.9055
CGAAGAG62150.066.259835
CGGAAGA4108000.065.7578054
TCGGAAG3843550.065.2529453
ATCGGAA3948800.063.5531852
ACGGAAG68700.063.1374133
GATCGGA4018350.061.864121
GATTGGA90900.060.2257421
CCGAAGA68000.060.0831644
TTGGAAG91200.059.698383
AATCATA1322100.058.6454855
ATTGGAA94600.057.8069462
Produced by FastQC (version 0.11.2)