FastQCFastQC Report
Mon 13 Apr 2015
2112_lane1_CAGATC_L001_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_CAGATC_L001_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC226611914.163243750000001TruSeq Adapter, Index 7 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1471770.91985625TruSeq Adapter, Index 7 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT1186760.741725TruSeq Adapter, Index 7 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC915750.57234375TruSeq Adapter, Index 7 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC646790.40424375TruSeq Adapter, Index 7 (98% over 50bp)
GATCCGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC453940.2837125TruSeq Adapter, Index 7 (98% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC370670.23166875TruSeq Adapter, Index 7 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC367050.22940625TruSeq Adapter, Index 7 (98% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCT195090.12193124999999999TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGATCATCTCGTATGC183850.11490624999999999TruSeq Adapter, Index 7 (98% over 50bp)
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC180870.11304375No Hit
CGCAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT162640.10165TruSeq Adapter, Index 7 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA190650.086.4925461
CCGGAAG195000.086.14213
ACCGGAA202150.083.027632
TCCGAAG73100.075.25393
CGAAGAG86700.074.5057455
ACGGAAG99350.072.966663
TTGGAAG140500.072.728043
GATTGGA139100.072.61011
GAGCACA4131050.072.0921869
ATTGGAA142900.071.442552
AGAGCAC4174200.071.388138
CATCGGA60600.071.0258561
AAGAGCA4247450.070.244227
GAAGAGC4266500.069.962866
TGGAAGA160450.069.275774
CCGAAGA91850.069.247914
GGAAGAG4277200.068.082375
CGGAAGA4210000.066.766934
GAACGGA106900.066.394751
GCGGAAG66700.065.523913
Produced by FastQC (version 0.11.2)