FastQCFastQC Report
Mon 13 Apr 2015
2112_lane1_CAGATC_L001_R1_003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_CAGATC_L001_R1_003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7780221
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC112003814.395966387073067TruSeq Adapter, Index 7 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC695460.8938820632473036TruSeq Adapter, Index 7 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT606300.7792837761292385TruSeq Adapter, Index 7 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC440800.5665648829255622TruSeq Adapter, Index 7 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC291960.37525926320087827TruSeq Adapter, Index 7 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC241440.31032537507610647TruSeq Adapter, Index 7 (98% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC157040.20184516609489628TruSeq Adapter, Index 7 (98% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCT97820.12572907633343577TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGATCATCTCGTATGC94770.12180887920792995TruSeq Adapter, Index 7 (98% over 50bp)
CGCAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT94280.12117907704678312TruSeq Adapter, Index 7 (98% over 50bp)
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC89830.11545944517514348No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA87000.086.496971
CCGGAAG92850.086.0598753
ACCGGAA96050.083.3421552
CATCGGA37000.074.728491
GAGCACA2020500.071.906549
GATTGGA67750.071.454551
TTGGAAG68500.071.433723
AGAGCAC2042000.071.2099158
ACGGAAG48050.070.5929643
ATTGGAA69900.070.2077942
AAGAGCA2078300.070.0529867
GAAGAGC2081150.070.0163966
TGGAAGA75600.069.679494
GGAAGAG2085500.068.125875
GAACGGA47700.066.829641
CGGAAGA2050250.066.6890644
TCGGAAG1891850.065.312093
GCGGAAG31400.064.7546163
TCTGAAG41050.064.5769653
ATCGGAA1933900.063.5292172
Produced by FastQC (version 0.11.2)