FastQCFastQC Report
Mon 13 Apr 2015
2112_lane1_GCCAAT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_GCCAAT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC15814909.8843125TruSeq Adapter, Index 6 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT1044100.6525625TruSeq Adapter, Index 6 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC922420.5765125TruSeq Adapter, Index 6 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC414090.25880625TruSeq Adapter, Index 6 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC347860.21741249999999998TruSeq Adapter, Index 6 (98% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCT239090.14943125TruSeq Adapter, Index 6 (100% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC222560.1391TruSeq Adapter, Index 6 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA126150.083.1677551
CCGGAAG132700.081.478443
ACCGGAA137200.079.0189742
GAGCACA2899250.071.883959
AGAGCAC2915600.071.5034268
GAAGAGC2965000.070.419436
AAGAGCA2973700.070.175087
GGAAGAG2961100.068.780035
TCCGAAG55700.068.656563
CGGAAGA2914300.068.2618564
TCGGAAG2658050.068.107413
ATCGGAA2723900.066.475752
AATCATA2241100.065.4762345
TAAATCA2084950.065.166883
ATAAATC2093500.064.979882
GATCGGA2744700.064.7435761
ACGGAAG71000.064.6338653
GAACGGA67700.064.431341
GATCCGA53000.064.371221
TCATAAA2373550.064.111577
Produced by FastQC (version 0.11.2)