FastQCFastQC Report
Mon 13 Apr 2015
2112_lane1_TGACCA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_TGACCA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9188940
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC440588847.9477284648719TruSeq Adapter, Index 4 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC2448132.6642137177955236TruSeq Adapter, Index 4 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGT2347512.5547125130863844TruSeq Adapter, Index 4 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1371141.4921634051370454TruSeq Adapter, Index 4 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC973841.059795797991934TruSeq Adapter, Index 4 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC634380.6903734271852902TruSeq Adapter, Index 4 (98% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC562310.6119421826674241TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAACAATCTCGTATGC547850.5962058735828071TruSeq Adapter, Index 4 (98% over 50bp)
GATCCGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC449320.4889791423167416TruSeq Adapter, Index 4 (98% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCT336060.3657222704686286TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGCAATCTCGTATGC313470.34113836851693446TruSeq Adapter, Index 4 (98% over 50bp)
CGTAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGT270940.2948544663475874TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACTAATCTCGTATGC226330.24630697338321939TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAACTCGTATGCC224830.24467457617527158TruSeq Adapter, Index 4 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCATCTCGTATGCC224760.24459839763890068TruSeq Adapter, Index 4 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCCATCTCGTATGC144110.1568298410915731TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACAAATCTCGTATGC124770.13578279975709928TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATTCGTATGCC113750.12379012160270933TruSeq Adapter, Index 4 (97% over 41bp)
GACGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCT105710.11504047256810906TruSeq Adapter, Index 4 (98% over 50bp)
CGAAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGT95240.1036463400566333TruSeq Adapter, Index 4 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAG296900.090.816743
ACCGGAA297800.090.307432
GACCGGA290300.089.998291
ATTGGAA169900.088.638192
GATTGGA172050.086.76591
TTGGAAG173950.086.652163
TGGAAGA196550.082.631494
TCCGAAG66950.080.037263
TCGGAAG6077900.079.7364653
ATCGGAA6109950.079.230352
CATCGGA88500.078.970881
GAAGAGC7032050.078.766476
GATCGGA6065800.078.5075761
GGAAGAG6949100.078.367725
AGAGCAC7062150.078.309038
GAGCACA7064350.078.252369
CGGAAGA6774050.078.069154
AAGAGCA7094000.078.0464867
ACGGAAG143250.076.902393
GCGGAAG90650.075.514433
Produced by FastQC (version 0.11.2)