FastQCFastQC Report
Mon 13 Apr 2015
2212_lane2_CTTGTA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2212_lane2_CTTGTA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC172770.10798125TruSeq Adapter, Index 12 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAAAT1313150.072.68661
ATAAATC1381850.069.946852
TAAATCA1503250.064.7113953
TCATAAA1682100.058.8418437
AATCATA1716150.057.768475
ATCATAA1847500.054.1447076
AAATCAT1806750.053.9462664
CATAAAC2125550.046.7557148
ATAAACA2283850.043.5066269
GTCCCCA34550.033.0160371
ACAAACG1756950.029.23134412-13
AAACGGG1842900.027.51751514-15
CAAACGG1817650.026.89238714-15
TGATACG28100.026.876513
CCCCGCA55750.026.0878491
CCCCCGA51000.025.6285631
ACGGGCA1999750.025.37699716-17
GGGCAAC2055750.024.408618-19
AACGGGC1969250.024.40483716-17
CGGGCAA2052400.023.24962418-19
Produced by FastQC (version 0.11.2)