FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2112_lane1_ATCACG_L001_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2112_lane1_ATCACG_L001_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13551447
Sequences flagged as poor quality0
Sequence length1-101
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCATA1561650.059.5556225
ATCATAA1641350.057.541256
CATAAAC1639300.057.107778
TCATAAA1651100.056.8090177
TAAATCA1483750.056.722063
AAATCAT1639100.055.4509244
ATAAATC1544050.054.5641172
ATAAACA2102800.044.6606839
AATAAAT1978450.042.4511031
AGAGCAC92100.038.62689694-95
AAACGGG1348000.035.52205714-15
ACGGGCA1360950.035.04606216-17
GGGCAAC1366500.034.48061818-19
CAAACGG1351700.034.43658414-15
AACGGGC1351900.033.73505816-17
CGGGCAA1363250.033.2685518-19
GCAACAT1431650.033.04536420-21
ACAAACG1497650.032.5040812-13
GGCAACA1410650.031.92548620-21
CATACCT1542950.030.04963924-25
Produced by FastQC (version 0.11.2)