##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	20150414_trimmed_2212_lane2_CTTGTA_L002_R1_004.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10548081
Sequences flagged as poor quality	0
Sequence length	1-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.5025988139454	31.0	28.0	34.0	16.0	34.0
2	29.13331547856622	31.0	28.0	34.0	16.0	34.0
3	30.162516119771634	31.0	30.0	34.0	25.0	34.0
4	33.53766978678323	35.0	33.0	37.0	28.0	37.0
5	33.79854507857608	35.0	35.0	37.0	28.0	37.0
6	34.80618892322656	36.0	35.0	37.0	32.0	37.0
7	35.03607557581795	36.0	35.0	37.0	32.0	37.0
8	35.09901207745753	37.0	35.0	37.0	32.0	37.0
9	36.79236480940801	39.0	37.0	39.0	32.0	39.0
10-11	36.798934935316105	39.0	37.0	39.0	32.0	39.0
12-13	36.899217650265484	39.0	37.0	39.0	33.0	39.0
14-15	38.065037505730814	40.0	37.0	41.0	33.0	41.0
16-17	38.02909994888171	40.0	37.0	41.0	33.0	41.0
18-19	38.25248808907852	40.0	37.5	41.0	33.5	41.0
20-21	38.476346073570426	40.0	38.0	41.0	34.0	41.0
22-23	38.48782312119167	40.0	38.0	41.0	34.0	41.0
24-25	38.45061730509457	40.0	38.0	41.0	34.0	41.0
26-27	38.322092408601335	40.0	38.0	41.0	34.0	41.0
28-29	38.215987094694626	40.0	38.0	41.0	33.5	41.0
30-31	38.175030262031015	40.0	38.0	41.0	33.0	41.0
32-33	38.09764020930761	40.0	37.0	41.0	33.0	41.0
34-35	38.06059839076009	40.0	37.0	41.0	33.0	41.0
36-37	38.02083525611645	40.0	37.0	41.0	33.0	41.0
38-39	37.96653011504306	40.0	37.0	41.0	33.0	41.0
40-41	37.90075800283697	40.0	37.0	41.0	33.0	41.0
42-43	37.82249275816023	40.0	37.0	41.0	33.0	41.0
44-45	37.73224903227805	40.0	36.5	41.0	33.0	41.0
46-47	37.64561574908319	40.0	36.0	41.0	32.0	41.0
48-49	37.55115191865667	40.0	36.0	41.0	32.0	41.0
50-51	37.43830639923979	39.5	35.0	41.0	32.0	41.0
52-53	37.33833872427314	39.0	35.0	41.0	32.0	41.0
54-55	37.19805127164233	39.0	35.0	41.0	31.0	41.0
56-57	37.029273998845596	39.0	35.0	41.0	31.0	41.0
58-59	36.87728992406357	39.0	35.0	41.0	31.0	41.0
60-61	36.6291343354267	38.0	35.0	40.0	31.0	41.0
62-63	36.39903618331818	37.5	35.0	40.0	31.0	41.0
64-65	36.10182062906861	37.0	34.5	39.5	31.0	41.0
66-67	35.740864367578055	36.5	34.0	39.0	30.5	41.0
68-69	35.377017426893424	36.0	34.0	39.0	30.0	40.0
70-71	35.046356495598026	35.0	34.0	37.5	30.0	39.0
72-73	34.70797081171125	35.0	34.0	37.0	30.0	39.0
74-75	34.385707021580345	35.0	34.0	36.5	30.0	39.0
76-77	32.556480944396185	33.5	31.0	35.0	27.0	36.5
78-79	33.68918079059385	35.0	33.0	35.5	29.5	37.0
80-81	33.73264357991785	35.0	33.0	35.0	30.0	36.5
82-83	33.63340805987761	35.0	33.0	35.0	30.0	36.0
84-85	33.55101933041316	35.0	33.0	35.0	30.0	36.0
86-87	33.45980015955148	35.0	33.0	35.0	30.0	36.0
88-89	33.37448532406452	35.0	33.0	35.0	30.0	35.0
90-91	33.34107970967279	35.0	33.0	35.0	30.0	35.0
92-93	33.287058657401275	35.0	33.0	35.0	30.0	35.0
94-95	33.27702049498768	35.0	33.0	35.0	30.0	35.0
96-97	33.279728424119405	35.0	33.0	35.0	30.0	35.0
98-99	33.339369821366645	35.0	33.0	35.0	30.0	35.0
100-101	32.671573421040165	34.5	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
2302	1	-4.875157547111307
2302	2	-5.408480517143673
2302	3	-2.4786950176854496
2302	4	-3.5063770051953504
2302	5	-3.5897725060484653
2302	6	-1.7436200742793417
2302	7	-0.8026197516902158
2302	8	-0.41889305006355926
2302	9	-0.29131701755927963
2302	10-11	-0.18968202059507888
2302	12-13	-0.3586682935026033
2302	14-15	-0.49408917959355136
2302	16-17	-0.5196292275812766
2302	18-19	-0.406226634286206
2302	20-21	-0.6373763596439161
2302	22-23	-0.43449296202780374
2302	24-25	-0.4365940209176671
2302	26-27	-0.6355123577918249
2302	28-29	-0.5996424187759359
2302	30-31	-0.44937100644949624
2302	32-33	-0.45023114734639336
2302	34-35	-0.44908567756034756
2302	36-37	-0.48355101155377866
2302	38-39	-0.5093847089804839
2302	40-41	-0.3976181067589266
2302	42-43	-0.33193463180944605
2302	44-45	-0.39931756791113315
2302	46-47	-0.5032003860757683
2302	48-49	-0.6329260003018362
2302	50-51	-0.5574009696886577
2302	52-53	-0.5648142749180209
2302	54-55	-0.5081978589831806
2302	56-57	-0.4328492337542471
2302	58-59	-0.4910119213630537
2302	60-61	-0.5916803997784115
2302	62-63	-0.5629772358010499
2302	64-65	-0.4934947985384923
2302	66-67	-0.5207040321287408
2302	68-69	-0.47007151402932834
2302	70-71	-0.5170446006919889
2302	72-73	-0.5226219553889493
2302	74-75	-0.46376693421922255
2302	76-77	-0.4986115064976744
2302	78-79	-0.3885949808817486
2302	80-81	-0.28893271714348856
2302	82-83	-0.31767906630059173
2302	84-85	-0.4080809837549566
2302	86-87	-0.3464733431151572
2302	88-89	-0.32053241799511056
2302	90-91	-0.4074824938342445
2302	92-93	-0.39561236155851986
2302	94-95	-0.34931919049575555
2302	96-97	-0.41289818720071025
2302	98-99	-0.34027643523202755
2302	100-101	-0.18578035734722675
2306	1	-2.9082825377399466
2306	2	-4.431216787496364
2306	3	-3.6860983724314274
2306	4	-3.3164079098959647
2306	5	-3.870552536198346
2306	6	-2.2267042714477654
2306	7	-0.8867474883014452
2306	8	-0.40751606667841855
2306	9	-0.2948151116345201
2306	10-11	-0.20084542698850072
2306	12-13	-0.24234937699666403
2306	14-15	-0.2609329222795296
2306	16-17	-0.31401191369587167
2306	18-19	-0.4762443262702405
2306	20-21	-0.7203987106551892
2306	22-23	-0.5978952910628266
2306	24-25	-0.5824157274398942
2306	26-27	-0.5527038675557421
2306	28-29	-0.6996918471529483
2306	30-31	-0.8707327373562634
2306	32-33	-0.8030232003102569
2306	34-35	-0.7914793209710567
2306	36-37	-0.6988158343604098
2306	38-39	-0.6264483411766122
2306	40-41	-0.6475130074252036
2306	42-43	-0.743941152910665
2306	44-45	-0.7092279546810545
2306	46-47	-0.6864828985702047
2306	48-49	-0.6712236160704776
2306	50-51	-0.7236804308693152
2306	52-53	-0.7457881546176282
2306	54-55	-0.6626011192771131
2306	56-57	-0.6804606673531879
2306	58-59	-0.699688306284969
2306	60-61	-0.6800209902110907
2306	62-63	-0.6237550692091318
2306	64-65	-0.6442162330295105
2306	66-67	-0.6487421076043702
2306	68-69	-0.5806538441142095
2306	70-71	-0.5705134489413126
2306	72-73	-0.59541618755798
2306	74-75	-0.5028074193332657
2306	76-77	-0.949859231032633
2306	78-79	-0.514706423427171
2306	80-81	-0.4369062709403906
2306	82-83	-0.42649413025599614
2306	84-85	-0.46612118479615816
2306	86-87	-0.40586834840267016
2306	88-89	-0.44041953536319056
2306	90-91	-0.46303242108294995
2306	92-93	-0.42487461507305113
2306	94-95	-0.37238493482568913
2306	96-97	-0.35234320542471664
2306	98-99	-0.3704816116329255
2306	100-101	-0.31012795367360724
2303	1	3.593987303335588
2303	2	3.3618768171231075
2303	3	2.3434206648513687
2303	4	2.360798500744373
2303	5	2.364388295470768
2303	6	1.2644466612688632
2303	7	0.5984482447398207
2303	8	0.2031705226114653
2303	9	0.12152774844725656
2303	10-11	0.07119734578514425
2303	12-13	0.13471016059667562
2303	14-15	0.08665012425323937
2303	16-17	0.1194160824013224
2303	18-19	0.3158032526244057
2303	20-21	0.6123106679273107
2303	22-23	0.5549916690275225
2303	24-25	0.478038994235078
2303	26-27	0.5429536366077699
2303	28-29	0.5687686882228249
2303	30-31	0.572426542920077
2303	32-33	0.597498592481692
2303	34-35	0.5998060438545139
2303	36-37	0.6000493177242774
2303	38-39	0.6077493429372467
2303	40-41	0.5518582921294097
2303	42-43	0.6124585752210123
2303	44-45	0.6220414017286586
2303	46-47	0.5396243604381965
2303	48-49	0.5677364758970924
2303	50-51	0.5901202151059124
2303	52-53	0.5281307084769722
2303	54-55	0.5275815514003455
2303	56-57	0.5530064706376123
2303	58-59	0.5647566774134418
2303	60-61	0.5896483818925731
2303	62-63	0.5644701107376235
2303	64-65	0.5050387977770256
2303	66-67	0.46609143850340473
2303	68-69	0.4814136419364985
2303	70-71	0.4140140609328711
2303	72-73	0.3724909138320882
2303	74-75	0.36318753654314406
2303	76-77	0.39759828566442224
2303	78-79	0.34640551134716446
2303	80-81	0.2368349119695452
2303	82-83	0.24056388088612835
2303	84-85	0.24120281458086623
2303	86-87	0.2081631229812828
2303	88-89	0.1876100626232784
2303	90-91	0.22827643139202536
2303	92-93	0.19807856581576289
2303	94-95	0.21034002376444505
2303	96-97	0.2383621880147757
2303	98-99	0.23603239087364614
2303	100-101	0.11441566222022459
2307	1	3.3014447323749003
2307	2	3.1462672704725527
2307	3	2.2084109259422355
2307	4	1.2777753924039175
2307	5	2.0371859902495117
2307	6	1.060031242217164
2307	7	0.4396819948904067
2307	8	0.2533850585567876
2307	9	0.2323804857315892
2307	10-11	0.08801549435992939
2307	12-13	0.14393267787718855
2307	14-15	0.09981290902435092
2307	16-17	0.14686473089228969
2307	18-19	0.21719258162299582
2307	20-21	0.4984082615467642
2307	22-23	0.4523060755881332
2307	24-25	0.45676016862466895
2307	26-27	0.4805684547623841
2307	28-29	0.4703988968939754
2307	30-31	0.5520205632147821
2307	32-33	0.5900910847386953
2307	34-35	0.5376600352153815
2307	36-37	0.5290476665915378
2307	38-39	0.5441512868746798
2307	40-41	0.5040661285984527
2307	42-43	0.5257367065213856
2307	44-45	0.5695733312670939
2307	46-47	0.5525177703428668
2307	48-49	0.5080672255482881
2307	50-51	0.5121122606567141
2307	52-53	0.5052365498627367
2307	54-55	0.46666998023488304
2307	56-57	0.45099089306552287
2307	58-59	0.46429482965974245
2307	60-61	0.45781480722457957
2307	62-63	0.438071097740071
2307	64-65	0.3806453325078394
2307	66-67	0.39193119522226283
2307	68-69	0.41203010828981235
2307	70-71	0.34896838063150426
2307	72-73	0.3513639561254607
2307	74-75	0.33962566119645743
2307	76-77	0.3659608922316764
2307	78-79	0.33456881651196113
2307	80-81	0.2382827999729571
2307	82-83	0.2421643114195291
2307	84-85	0.23999077148874193
2307	86-87	0.18003163552220514
2307	88-89	0.15737561788378684
2307	90-91	0.14603382489280392
2307	92-93	0.13171801839995112
2307	94-95	0.13861200047468003
2307	96-97	0.14693231408273277
2307	98-99	0.16029956271392365
2307	100-101	0.10793894594493736
2304	1	3.261326790563391
2304	2	3.166202172802027
2304	3	2.2492324103184878
2304	4	2.145269349883158
2304	5	2.3265725064722957
2304	6	1.2119273031311906
2304	7	0.5539438316285867
2304	8	0.19140007528974223
2304	9	0.0798704512954771
2304	10-11	0.06323129955052309
2304	12-13	0.18141200879793473
2304	14-15	0.14547188479855322
2304	16-17	0.16509940772640164
2304	18-19	0.24115592676233177
2304	20-21	0.5218578145865678
2304	22-23	0.4027405823461905
2304	24-25	0.4629543162931711
2304	26-27	0.39730190559986056
2304	28-29	0.43775414666333035
2304	30-31	0.5057180452019878
2304	32-33	0.4657073279416224
2304	34-35	0.4948470672983376
2304	36-37	0.5443725106498576
2304	38-39	0.47341989763211956
2304	40-41	0.49262877390371784
2304	42-43	0.47940846312598495
2304	44-45	0.44662541870292927
2304	46-47	0.4834817177801085
2304	48-49	0.4277065421322561
2304	50-51	0.4407504427665785
2304	52-53	0.49305499176139733
2304	54-55	0.4516528927306567
2304	56-57	0.4028594930018343
2304	58-59	0.4517257015846994
2304	60-61	0.41528109491751763
2304	62-63	0.3613234307876567
2304	64-65	0.3519837218273665
2304	66-67	0.3665600095340338
2304	68-69	0.3594477658884472
2304	70-71	0.35399158274639575
2304	72-73	0.3225892755528932
2304	74-75	0.2936320438065394
2304	76-77	0.3470763619780328
2304	78-79	0.27481439144309405
2304	80-81	0.24948461415579715
2304	82-83	0.2224628847859691
2304	84-85	0.1833900082830624
2304	86-87	0.18430625485413543
2304	88-89	0.1551892789943281
2304	90-91	0.19982195247835222
2304	92-93	0.2226094186521408
2304	94-95	0.21872645633198573
2304	96-97	0.20214456880002274
2304	98-99	0.22798547027245775
2304	100-101	0.15206247423694919
2301	1	3.141847747529493
2301	2	3.092990814888527
2301	3	2.1771827528778154
2301	4	1.4536175879824285
2301	5	2.0446011598497904
2301	6	1.061504465140814
2301	7	0.47563884061182904
2301	8	0.2500064589330293
2301	9	0.061254781314360685
2301	10-11	0.0181130950971351
2301	12-13	0.10970760248540756
2301	14-15	0.1670201707674721
2301	16-17	0.22183969446616914
2301	18-19	0.2740960470465126
2301	20-21	0.44906429076188914
2301	22-23	0.3620093125395485
2301	24-25	0.37335086069712276
2301	26-27	0.364648358164807
2301	28-29	0.43676833557706374
2301	30-31	0.46839702499895
2301	32-33	0.3903759178395134
2301	34-35	0.4533810191994121
2301	36-37	0.42177211832881056
2301	38-39	0.4099653994753041
2301	40-41	0.4266399157730305
2301	42-43	0.38888012533809047
2301	44-45	0.4606816898407189
2301	46-47	0.3812285599797889
2301	48-49	0.4018956047560067
2301	50-51	0.4060090194766701
2301	52-53	0.41951350322361236
2301	54-55	0.44095071621823934
2301	56-57	0.3831719333114876
2301	58-59	0.4050228445299666
2301	60-61	0.43278861874605923
2301	62-63	0.3575826333126102
2301	64-65	0.3353127937193676
2301	66-67	0.37248241760699585
2301	68-69	0.3447358805694165
2301	70-71	0.34733232696406446
2301	72-73	0.35376313437082985
2301	74-75	0.2754191380210216
2301	76-77	0.3546673133215492
2301	78-79	0.29040338141507505
2301	80-81	0.2306376006169657
2301	82-83	0.23661677150104765
2301	84-85	0.26782365474891634
2301	86-87	0.2719124372904602
2301	88-89	0.26491224611264386
2301	90-91	0.2611199613926516
2301	92-93	0.18898790748147576
2301	94-95	0.1935251679315968
2301	96-97	0.2212970812356474
2301	98-99	0.20047117916430324
2301	100-101	0.13878242833909127
2305	1	3.507867318689833
2305	2	3.2101853490257604
2305	3	2.2614156815512025
2305	4	2.6105109815497585
2305	5	2.384896940754807
2305	6	1.223827905806374
2305	7	0.5619558426538873
2305	8	0.17538760683347476
2305	9	0.11670879381684074
2305	10-11	0.027321943875797672
2305	12-13	0.14371860776981293
2305	14-15	0.1330888429077106
2305	16-17	0.08861154158812212
2305	18-19	0.1136062659578414
2305	20-21	0.3556487371148549
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2313	40-41	-0.1791199749060297
2313	42-43	-0.21227805860561233
2313	44-45	-0.2098768194704732
2313	46-47	-0.25175934643702647
2313	48-49	-0.17647901899039198
2313	50-51	-0.20038031407793255
2313	52-53	-0.15271457527821042
2313	54-55	-0.2559316373269809
2313	56-57	-0.2576341729408256
2313	58-59	-0.18255832633299462
2313	60-61	-0.2200498017400747
2313	62-63	-0.24653911344609725
2313	64-65	-0.24145478392041753
2313	66-67	-0.1874947567658154
2313	68-69	-0.19711054074169
2313	70-71	-0.14044073614500974
2313	72-73	-0.15217512612345985
2313	74-75	-0.22798560145568558
2313	76-77	-0.2080333619951915
2313	78-79	-0.1312080329221672
2313	80-81	-0.12088360483473792
2313	82-83	-0.14143885283785806
2313	84-85	-0.13422191952216878
2313	86-87	-0.09570081989138401
2313	88-89	-0.10883371243112094
2313	90-91	-0.08187405360614264
2313	92-93	-0.10341334463252139
2313	94-95	-0.11542509867408057
2313	96-97	-0.05847022567719051
2313	98-99	-0.04588658147987701
2313	100-101	-0.07405614203761246
2316	1	-3.508066436788578
2316	2	-2.7568800353546017
2316	3	-2.336464634324379
2316	4	-2.6575208954422713
2316	5	-2.311557893835115
2316	6	-1.1377429313663185
2316	7	-0.5090335254649858
2316	8	-0.26856696938781255
2316	9	-0.19556956382006518
2316	10-11	-0.10732889879221119
2316	12-13	-0.11939391443628722
2316	14-15	-0.09291065374041807
2316	16-17	-0.08254438320456359
2316	18-19	-0.12140361844907943
2316	20-21	-0.4094275452706242
2316	22-23	-0.3291623826993302
2316	24-25	-0.21055016674170446
2316	26-27	-0.1992331993604992
2316	28-29	-0.2880864071233802
2316	30-31	-0.29190175645662464
2316	32-33	-0.2639845814775512
2316	34-35	-0.36064592365260495
2316	36-37	-0.4038855515854678
2316	38-39	-0.3370715315671262
2316	40-41	-0.34100201292586974
2316	42-43	-0.4071458153281782
2316	44-45	-0.35598621592934165
2316	46-47	-0.25316744978887584
2316	48-49	-0.2604313445758706
2316	50-51	-0.2550653564046357
2316	52-53	-0.2930644581424744
2316	54-55	-0.28408211092819613
2316	56-57	-0.2568029952772548
2316	58-59	-0.2978312165376309
2316	60-61	-0.32940771417931103
2316	62-63	-0.3226545484872858
2316	64-65	-0.30568595814251864
2316	66-67	-0.2651604538392789
2316	68-69	-0.25414012425375176
2316	70-71	-0.2041617024197464
2316	72-73	-0.22980716963827064
2316	74-75	-0.19539699078794115
2316	76-77	-0.18125092939433074
2316	78-79	-0.2619968421709302
2316	80-81	-0.26220647643717854
2316	82-83	-0.2568557542363479
2316	84-85	-0.21486817913145018
2316	86-87	-0.15910538956349995
2316	88-89	-0.15066353299435775
2316	90-91	-0.18386835591761752
2316	92-93	-0.12293298467923819
2316	94-95	-0.16644876316172486
2316	96-97	-0.17433823709694707
2316	98-99	-0.09749279100059738
2316	100-101	-0.13463695319060065
2216	1	-1.5578167860368275
2216	2	-2.2777991748685693
2216	3	-0.6303737487694079
2216	4	-1.408500016718758
2216	5	-3.012136127733612
2216	6	-1.2696573391007249
2216	7	-0.646465189070426
2216	8	-0.3510757527457784
2216	9	-0.23871164275116996
2216	10-11	-0.19157851438000506
2216	12-13	-0.17629363078793148
2216	14-15	-0.23981056530824674
2216	16-17	-0.223780763912643
2216	18-19	-0.24248793144741398
2216	20-21	-0.7790264941536122
2216	22-23	-0.5459386539899569
2216	24-25	-0.43421497774702544
2216	26-27	-0.4375227933037067
2216	28-29	-0.42251955065167834
2216	30-31	-0.4220260230940198
2216	32-33	-0.3766938092862304
2216	34-35	-0.4118722445271459
2216	36-37	-0.5452943018154031
2216	38-39	-0.44829591209005315
2216	40-41	-0.4883277148263403
2216	42-43	-0.4808629509636688
2216	44-45	-0.4510559736682467
2216	46-47	-0.5064570116149483
2216	48-49	-0.49128277886956795
2216	50-51	-0.5260566790699812
2216	52-53	-0.5141282519945776
2216	54-55	-0.49814535245585034
2216	56-57	-0.500613023137241
2216	58-59	-0.5022938261009102
2216	60-61	-0.5009199087010785
2216	62-63	-0.481915789121679
2216	64-65	-0.45121785153909855
2216	66-67	-0.434671600009942
2216	68-69	-0.4088378629683618
2216	70-71	-0.3847184415365774
2216	72-73	-0.30595890962359107
2216	74-75	-0.3904329949473464
2216	76-77	-0.34915639565162593
2216	78-79	-0.3645054744806515
2216	80-81	-0.2829999182384526
2216	82-83	-0.2648575374601876
2216	84-85	-0.22964593656556787
2216	86-87	-0.27531551458148584
2216	88-89	-0.24448393000919566
2216	90-91	-0.2228556330237339
2216	92-93	-0.1824167076254497
2216	94-95	-0.24103247778332104
2216	96-97	-0.24962812710227666
2216	98-99	-0.21891022912625147
2216	100-101	-0.18622407525459295
2215	1	-2.574260127586669
2215	2	-2.8974939861974676
2215	3	-1.0411690515894527
2215	4	-0.2783910312857003
2215	5	-2.4744934823982057
2215	6	-1.3171484165864271
2215	7	-0.8007738679752165
2215	8	-0.4425178508668637
2215	9	-0.4852944107890025
2215	10-11	-0.4526458965997193
2215	12-13	-0.587632163908097
2215	14-15	-0.5479134880170591
2215	16-17	-0.6151017434674202
2215	18-19	-0.6652398471550782
2215	20-21	-0.6270974068908544
2215	22-23	-0.7548434610807107
2215	24-25	-0.8481560532576893
2215	26-27	-0.8305608859817539
2215	28-29	-0.7584873504209853
2215	30-31	-0.7990057011082214
2215	32-33	-0.8502216104844322
2215	34-35	-0.8005917338467299
2215	36-37	-0.7577255985675251
2215	38-39	-0.8482627843883037
2215	40-41	-0.7243467935292927
2215	42-43	-0.7606814742940031
2215	44-45	-0.8730916008562843
2215	46-47	-0.824970235704825
2215	48-49	-0.7818401883007553
2215	50-51	-0.7682944951582868
2215	52-53	-0.7582764645286062
2215	54-55	-0.76862293830402
2215	56-57	-0.7699590169088282
2215	58-59	-0.8565289244588428
2215	60-61	-0.997141830606779
2215	62-63	-0.7705160070495864
2215	64-65	-0.7147381652975398
2215	66-67	-0.6867981007930837
2215	68-69	-0.7108210157478609
2215	70-71	-0.6458587360157964
2215	72-73	-0.5773991720893505
2215	74-75	-0.5034978717940675
2215	76-77	-0.38066486844115843
2215	78-79	-0.5604777841291693
2215	80-81	-0.6314749814491947
2215	82-83	-0.5523367880243626
2215	84-85	-0.4281866631263469
2215	86-87	-0.5055980132828424
2215	88-89	-0.4129568329786224
2215	90-91	-0.5086966197878198
2215	92-93	-0.5072587770468431
2215	94-95	-0.44386822608868215
2215	96-97	-0.4073586275087635
2215	98-99	-0.445287614205526
2215	100-101	-0.33228468916911424
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	98.0
15	5193.0
16	2967.0
17	2985.0
18	1645.0
19	1385.0
20	1560.0
21	1424.0
22	1977.0
23	3260.0
24	7159.0
25	16630.0
26	38669.0
27	80512.0
28	141311.0
29	217716.0
30	301264.0
31	392161.0
32	500219.0
33	646282.0
34	869646.0
35	1211412.0
36	1601655.0
37	2120943.0
38	2142580.0
39	228714.0
40	8714.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.79740248486905	25.57395985108571	15.244005046984377	44.384632617060866
2	16.9964893997469	29.004556916132795	23.638907629535158	30.360046054585148
3	11.89009145772036	18.54789608182179	30.559986787523158	39.00202567293469
4	16.987480019693923	21.069114220970995	14.258058312528021	47.68534744680706
5	19.699076975689824	24.883975522533596	16.809114078661413	38.60783342311517
6	15.902449515182127	31.43553658157417	19.604245570697156	33.057768332546544
7	12.646924156182024	32.5514941215148	23.831686489143625	30.96989523315955
8	11.467839816772843	35.49262713900328	20.505887748677974	32.533645295545895
9	11.450278585950029	40.48706811484887	21.86453806853786	26.198115230663237
10-11	11.871660106122313	37.064587419572156	23.2623408192619	27.801411655043633
12-13	11.110311288446843	40.10241784649221	20.25230809815221	28.534962766908745
14-15	11.530272809576626	37.040766854745485	20.088849929377172	31.34011040630072
16-17	11.3380718889852	39.070963811152296	20.01577278892982	29.57519151093268
18-19	14.337445479429126	32.13343535234603	20.919483602456825	32.60963556576802
20-21	17.085845316389992	32.95113040188939	21.04052040195788	28.922503879762747
22-23	10.299540114668886	36.35570322583163	20.47711169681939	32.867644962680096
24-25	10.26191535920933	35.96338428850769	20.293266710818134	33.48143364146485
26-27	10.3008896871651	36.51015305037848	23.78930607224103	29.399651190215398
28-29	10.373026305796973	35.9578519191657	19.945704095295767	33.723417679741566
30-31	10.27085584035613	32.86594510493763	23.659655189100153	33.203543865606086
32-33	10.311647142072124	36.499681113638665	21.651966619390134	31.53670512489908
34-35	10.386758149505845	36.02972153482488	21.190383990926357	32.39313632474292
36-37	10.309586188351949	35.79374982880498	21.509854274937474	32.3868097079056
38-39	10.331738943203757	36.25922828113681	21.722397791778857	31.68663498388058
40-41	10.379204006903398	35.97800050850406	21.34091376744686	32.30188171714568
42-43	10.277898586573555	35.749758294621174	21.660944421984407	32.311398696820866
44-45	10.277082824186506	36.2289476522618	21.800844775768326	31.69312474778337
46-47	10.365230355025783	35.9302303858361	21.39845766501442	32.3060815941237
48-49	10.24463829754254	35.81190779591552	21.705252467558918	32.23820143898302
50-51	10.246037356525274	36.20553453801191	21.939280063047875	31.609148042414937
52-53	10.2770152051327	35.90755882534073	21.577601603100085	32.23782436642649
54-55	10.182538007859382	35.72931576374257	21.895809707347887	32.192336521050166
56-57	10.210751595585103	36.188616933157775	21.951203284031877	31.649428187225247
58-59	10.257361105894255	35.945658170259385	21.584140097314823	32.21284062653154
60-61	10.14322376173711	35.74964740616874	21.928729126897633	32.17839970519651
62-63	10.144458111000935	36.1415046497398	22.097913752990937	31.61612348626832
64-65	10.19634589840914	35.909682307020866	21.70271204198133	32.191259752588664
66-67	10.072758670198743	35.729989671504484	21.951403424723384	32.24584823357339
68-69	10.077473579269608	36.17499647815038	22.087725019265395	31.659804923314617
70-71	10.109939840616153	36.00692262029695	21.727486614329045	32.15565092475786
72-73	10.003467797101784	35.75305485716193	22.040866615586605	32.202610730149686
74-75	9.980406771385079	36.16170346564275	22.17188913308646	31.686000629885708
76-77	9.996087198795824	36.015204712112734	21.805308762830805	32.183399326260634
78-79	9.913032592074696	35.8177420737965	22.07652297989994	32.19270235422886
80-81	9.924529051902674	36.198876111201656	22.215839120393372	31.660755716502297
82-83	9.967131122314703	36.04225193336254	21.85684458774221	32.13377235658055
84-85	9.880273153425602	35.83329995920216	22.147420754889204	32.13900613248304
86-87	10.088052120478928	36.51481965938695	22.098254352653957	31.298873867480165
88-89	10.383640074359114	36.54158886222073	21.751273739009065	31.323497324411086
90-91	10.58518508329092	35.987520805535304	22.030581729644965	31.396712381528815
92-93	11.537214790162734	36.2052328565537	21.863705118667003	30.393847234616565
94-95	12.047739095248756	36.476740192363586	21.22420215835441	30.25131855403325
96-97	12.766651681563951	36.57883939071544	21.055053996818373	29.599454930902237
98-99	13.392445677108764	36.895510917287965	20.67940560440336	29.03263780119991
100-101	13.985157150971329	36.893638946367005	19.741302034252843	29.379901868408826
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2807.0
1	2788.5
2	2253.5
3	1411.0
4	1124.5
5	1172.5
6	1188.0
7	1181.0
8	1177.5
9	1197.5
10	1273.5
11	1342.0
12	1353.0
13	1846.0
14	2388.5
15	2491.0
16	2602.0
17	2991.0
18	3550.0
19	3962.5
20	4402.0
21	5090.5
22	5993.5
23	7309.0
24	9282.5
25	12602.0
26	18670.0
27	29693.5
28	49494.0
29	82006.0
30	132423.5
31	201569.5
32	288538.0
33	386223.0
34	483377.0
35	571152.0
36	638195.5
37	677380.0
38	686900.0
39	666576.0
40	621082.5
41	562301.0
42	498147.5
43	434440.5
44	377735.0
45	329201.5
46	288071.5
47	255756.0
48	230718.0
49	213023.5
50	203446.0
51	194250.5
52	183607.0
53	176661.5
54	173338.5
55	172093.0
56	171338.0
57	170282.5
58	168236.0
59	164854.5
60	158851.0
61	151195.0
62	143199.5
63	132998.5
64	121349.5
65	108978.5
66	96542.0
67	84797.0
68	73753.5
69	62932.0
70	52605.0
71	43565.0
72	36016.5
73	29835.5
74	24756.0
75	20580.5
76	17231.5
77	14764.0
78	12876.5
79	11188.5
80	9910.5
81	8955.0
82	8245.0
83	7489.0
84	6804.0
85	6480.5
86	6192.5
87	6007.0
88	5040.0
89	4088.5
90	3804.5
91	3516.0
92	3206.5
93	2841.5
94	2589.0
95	2347.5
96	2205.0
97	2133.0
98	2096.0
99	2082.5
100	2080.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005555518687304881
3	0.0
4	0.0025508140652835817
5	6.357595181739924E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	7.124911864840232E-5
12-13	0.0
14-15	0.0
16-17	1.3786552786571325E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.4305643409425845E-5
28-29	5.25391577925243E-5
30-31	0.013883402581250448
32-33	1.4390176689784485E-5
34-35	0.002159291563849843
36-37	0.001282740628398238
38-39	0.0
40-41	1.2605920030952383E-4
42-43	4.4244590709822295E-4
44-45	1.6091126879656947E-4
46-47	0.0
48-49	0.0030068247072699184
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.245401789781857E-4
60-61	0.006568754892510125
62-63	0.0
64-65	5.0364858141836905E-6
66-67	0.0
68-69	0.0
70-71	0.0
72-73	3.85024102508817E-4
74-75	8.785729948677384E-5
76-77	0.0
78-79	0.0033292173214871314
80-81	0.0
82-83	1.2859062531477914E-4
84-85	1.5737370421069625E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.006557865811689059
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-4	9505.0
5-9	11168.0
10-14	7751.0
15-19	7815.0
20-24	16200.0
25-29	42555.0
30-34	63259.0
35-39	70029.0
40-44	73188.0
45-49	75943.0
50-54	76880.0
55-59	83080.0
60-64	93027.0
65-69	111548.0
70-74	149251.0
75-79	199675.0
80-84	275218.0
85-89	84738.0
90-94	122742.0
95-99	249425.0
100-102	8725084.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.7132010851667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.978136871778	79.79983613788083
2	6.944541798245521	12.18255982387709
3	1.3823102080218956	3.6374055971490944
4	0.37422075665901977	1.3129640191630334
5	0.12835294485011364	0.5629123830755665
6	0.05231178031194937	0.2753060223375048
7	0.008155577944558064	0.05007462937517404
8	0.014277674997726185	0.10018724624833711
9	0.01587009995583602	0.12528155418011458
>10	0.09884120790174752	1.585913489120494
>50	0.0018994372228261427	0.11545960047059714
>100	9.918779175312209E-4	0.1485745305218322
>500	2.7073970939630074E-5	0.015934536649823625
>1k	6.269022223807504E-5	0.08759042995055817
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	4.3609828176328946E-4	0.0	0.0
2	4.3609828176328946E-4	0.0	0.0
3	4.550590766225629E-4	1.8960794859273454E-5	0.0
4	4.550590766225629E-4	1.8960794859273454E-5	9.480397429636727E-6
5	4.6453947405219965E-4	1.8960794859273454E-5	9.480397429636727E-6
6	4.835002689114731E-4	1.8960794859273454E-5	9.480397429636727E-6
7	4.835002689114731E-4	1.8960794859273454E-5	9.480397429636727E-6
8	4.929806663411099E-4	1.8960794859273454E-5	9.480397429636727E-6
9	5.024610637707465E-4	3.792158971854691E-5	9.480397429636727E-6
10-11	5.35642454774475E-4	3.792158971854691E-5	9.480397429636727E-6
12-13	5.640836470633852E-4	3.792158971854691E-5	9.480397429636727E-6
14-15	6.067454354967505E-4	3.792158971854691E-5	9.480397429636727E-6
16-17	6.351866277856607E-4	8.532357686673054E-5	9.480397429636727E-6
18-19	6.77848416219026E-4	9.480397429636726E-5	9.480397429636727E-6
20-21	7.157700059375728E-4	1.04284371726004E-4	9.480397429636727E-6
22-23	7.394709995116647E-4	1.04284371726004E-4	9.480397429636727E-6
24-25	7.916131853746667E-4	1.04284371726004E-4	9.480397429636727E-6
26-27	8.34274973808032E-4	1.04284371726004E-4	9.480397429636727E-6
28-29	9.006377558154891E-4	1.04284371726004E-4	9.480397429636727E-6
30-31	9.812211339674012E-4	1.04284371726004E-4	1.8960794859273454E-5
32-33	0.001052324114689677	1.04284371726004E-4	1.8960794859273454E-5
34-35	0.0010902457044082237	1.04284371726004E-4	1.8960794859273454E-5
36-37	0.001199270274849046	1.04284371726004E-4	1.8960794859273454E-5
38-39	0.001279853653000958	1.04284371726004E-4	1.8960794859273454E-5
40-41	0.0013509566337232336	1.04284371726004E-4	1.8960794859273454E-5
42-43	0.0014552410054492378	1.04284371726004E-4	1.8960794859273454E-5
44-45	0.0015784861720345151	1.1376476915564073E-4	1.8960794859273454E-5
46-47	0.0017633539219124312	1.1376476915564073E-4	1.8960794859273454E-5
48-49	0.0020240648512274413	1.1850496787045909E-4	1.8960794859273454E-5
50-51	0.0022610747869683595	1.2324516658527746E-4	1.8960794859273454E-5
52-53	0.0024459425368462753	1.2324516658527746E-4	1.8960794859273454E-5
54-55	0.002682952472587194	1.2324516658527746E-4	2.844119228891018E-5
56-57	0.0028915212160392015	1.2324516658527746E-4	6.162258329263873E-5
58-59	0.003085869363346755	1.2324516658527746E-4	6.63627820074571E-5
60-61	0.003351320491376583	1.2324516658527746E-4	6.63627820074571E-5
62-63	0.003725796189847234	1.2324516658527746E-4	6.63627820074571E-5
64-65	0.004019688510165972	1.2324516658527746E-4	6.63627820074571E-5
66-67	0.004398904407351441	1.2324516658527746E-4	6.63627820074571E-5
68-69	0.004877664477548097	1.2324516658527746E-4	6.63627820074571E-5
70-71	0.005764081637219131	1.3746576272973254E-4	6.63627820074571E-5
72-73	0.007252504033672096	1.422059614445509E-4	6.63627820074571E-5
74-75	0.010162986044570572	1.4694616015936929E-4	6.63627820074571E-5
76-77	0.013438463356510062	1.5168635887418763E-4	6.63627820074571E-5
78-79	0.017453411667961216	1.6116675630382436E-4	6.63627820074571E-5
80-81	0.022482762504383497	1.8012755116309783E-4	7.110298072227546E-5
82-83	0.027872368443131977	1.8960794859273456E-4	7.584317943709382E-5
84-85	0.034636632009177784	1.8960794859273456E-4	7.584317943709382E-5
86-87	0.8042932169368058	1.8960794859273456E-4	7.584317943709382E-5
88-89	1.9761272216244832	1.8960794859273456E-4	7.584317943709382E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATAAAT	85980	0.0	69.26979	1
ATAAATC	90450	0.0	67.12525	2
TAAATCA	98070	0.0	62.87407	3
TCATAAA	111235	0.0	56.847145	7
AATCATA	112630	0.0	55.85618	5
ATCATAA	121970	0.0	52.408173	6
AAATCAT	118555	0.0	52.259148	4
AGAGCAC	12615	0.0	47.526913	94-95
CATAAAC	139640	0.0	45.466774	8
ATAAACA	149325	0.0	42.483635	9
GAGCACA	7160	0.0	41.106224	94-95
AAGAGCA	19590	0.0	33.626514	94-95
GTCCCCA	2115	0.0	31.163923	1
ACAAACG	116970	0.0	28.008755	12-13
TGATACG	1565	0.0	27.514189	3
GTCCCCC	1485	0.0	26.87254	1
GAAGAGC	26635	0.0	26.340101	94-95
AAACGGG	123245	0.0	26.202904	14-15
CCCCGCA	3775	0.0	25.893139	1
CAAACGG	121160	0.0	25.802752	14-15
>>END_MODULE