FastQCFastQC Report
Wed 15 Apr 2015
20150414_trimmed_2212_lane2_GCCAAT_L002_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150414_trimmed_2212_lane2_GCCAAT_L002_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15944342
Sequences flagged as poor quality0
Sequence length1-101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAATC4449600.075.590352
AATAAAT4533450.073.208491
TAAATCA4693200.072.262853
AATCATA5068800.068.437935
TCATAAA5117750.067.921597
AAATCAT5070100.067.079684
ATCATAA5275450.066.379816
CATAAAC5550900.062.5122688
ATGAATC28550.059.0105822
ATAAACA6211600.055.8864979
AGAAATC49100.046.881752
GATAAAT55700.043.98831
GAATCAT40050.043.747194
GTAAATC23400.043.6992952
GTCCCCA17900.043.529791
TGCCCGT202050.040.9490471
AGTAAAT23500.040.4397161
TGAATCA42600.040.0656933
AATGAAT42300.039.8221551
AGAGCAC25700.039.56251594-95
Produced by FastQC (version 0.11.2)