FastQCFastQC Report
Wed 6 May 2015
20150506_trimmed_2212_lane2_CTTGTA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20150506_trimmed_2212_lane2_CTTGTA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15796545
Sequences flagged as poor quality0
Sequence length1-62
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA96350.051.95169456
AGAGCAC173050.029.56903656
AAGAGCA272850.021.16778456
GAAGAGC376750.016.36904156
GGAAGAG506800.013.29878856
AGTGTAC2909.673422E-712.07383639
CGGAAGA590650.011.26001156
TCGGAAG718600.011.14156456
ATCGGAA871250.010.11718656
CAGTGTA4401.4973375E-79.88113338
GATCGGA1015150.09.17519356
TACGGAG4351.0643324E-58.83601743
AGATCGG1186350.08.37418956
AAGATCG530350.07.67415756
TAGATCG385750.07.45789555
CTTAGAT52650.07.373678756
CCTAAGA25700.07.34392954
TGTACGG5251.17504154E-47.29122141
ATGTACC6251.2708915E-57.24877450
CAGATCG416700.07.16311756
Produced by FastQC (version 0.11.2)