FastQCFastQC Report
Wed 2 Apr 2014
mcf_M1_R1.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename mcf_M1_R1.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 8023643
Filtered Sequences 0
Sequence length 19-76
%GC 16

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CCCCC 275 2796533.2 1.8033162E7 16
ACCCC 140 10678.758 271014.66 22
CCCCA 135 10297.373 298283.8 23
CACCC 110 8390.452 135235.05 13
CCCAC 80 6102.147 82661.82 52
CGCCC 35 6076.6914 184691.5 15
CCACC 60 4576.6104 54977.043 49
CTCCC 65 4032.359 66183.6 24
CCTCC 60 3722.1775 44620.2 46
CGCGC 985 2919.7607 9094.343 71
CCCGC 15 2604.2966 62717.715 52
CCCCG 15 2604.2966 61969.664 33
GCCCC 15 2604.2964 65696.69 69
CCCTC 35 2171.2703 23791.188 71
TCCCC 35 2171.2703 45335.73 61
CCCCT 35 2171.2703 43976.152 1
CCGCC 10 1736.1978 62397.504 45
CACAC 1200 686.56134 6897.688 12
CGCGG 6930 350.71643 539.9176 24
CGCTC 315 333.6324 12012.933 2
CCGTC 310 328.3367 4580.34 49
TCGCG 17410 314.82437 433.79144 39
CGGCG 6170 312.25403 474.55365 50
CGCCG 100 296.42242 2103.413 19
GCGCG 5605 283.66028 534.4831 59
CGCGA 12505 278.0354 401.89743 10
CGTCG 13770 249.0024 429.42642 6
CCGCG 80 237.13794 2102.595 16
CGACG 10530 234.12337 363.39114 22
CGCGT 11440 206.86908 292.7774 64
ACGCG 8915 198.2156 315.42014 18
ACTCC 410 190.78099 2149.2883 22
CACGC 135 175.80786 2770.6543 13
ACACC 305 174.501 2434.4783 12
CCGGC 55 163.03233 2104.193 20
GCGCC 55 163.03233 1094.2274 65
CGGAC 7030 156.30458 269.25882 63
CTTCC 405 153.27078 4426.522 6
CGCAC 115 149.76225 1883.3408 53
ACCAC 260 148.75496 1838.8649 34
CTCCA 315 146.57564 2315.9202 23
CGGCC 45 133.39009 2136.4844 49
GCCGC 45 133.39009 1094.2274 65
ACGCC 100 130.22807 2308.879 14
CCCAG 95 123.716644 1852.4558 24
CCGAC 95 123.716644 935.5089 44
CCCAA 200 114.42688 1424.2277 24
CACCA 195 111.56621 1021.05237 33
CGTCC 105 111.210815 751.12854 15
CCACG 85 110.69384 942.56616 54
GCACC 85 110.69384 938.62744 49
CACTC 235 109.350075 1009.4084 53
AACCC 185 105.84487 2234.5474 21
CCAAC 175 100.12353 1220.7667 24
GCCCA 75 97.671036 929.63934 33
CCACA 165 94.402176 1035.2504 54
CACCT 200 93.063896 1162.1398 32
CAACC 160 91.54151 1018.05927 26
CAGCC 70 91.15965 943.3911 55
CTCGC 85 90.02779 765.8647 53
GGCCC 30 88.92673 2122.6462 39
CTCTC 225 85.15043 1505.0864 53
GTCCC 80 84.73205 1516.8907 39
ACCCA 145 82.95949 611.2903 28
TCCCA 175 81.43091 671.7982 51
GCGGC 1530 77.4309 179.37766 7
CCTAC 160 74.45112 681.472 62
CTGCC 70 74.14054 762.37555 47
TGCCC 70 74.14054 779.47656 64
CGGGC 1400 70.85181 183.14717 54
TCCGC 65 68.84478 753.8633 26
GCCTC 65 68.84478 392.6415 66
GCCCT 65 68.84478 765.2066 52
CCAGC 50 65.11402 1389.3418 24
GCCAC 50 65.11402 937.98206 48
AGCCC 50 65.11402 940.0743 51
CCCTA 135 62.81813 535.35614 71
CCCAT 135 62.81813 671.7982 51
TTCGC 9555 61.7372 89.370415 18
CCCTT 160 60.55142 407.00577 41
CTCAC 130 60.491524 672.9388 53
GCGTC 3290 59.492947 121.98936 21
GCCCG 20 59.284485 1085.295 62
TCCCT 155 58.659187 406.78864 40
TTCCC 155 58.659187 416.6441 63
TCGCC 55 58.253284 406.18887 71
GCTCC 55 58.253284 774.0206 61
CTCCG 55 58.25328 761.8042 46
CCACT 125 58.164936 1163.8109 35
TCGGC 3115 56.328426 102.88994 24
ACCTC 120 55.838337 1345.8778 53
CTACC 120 55.83833 1007.69727 51
ACCCT 115 53.51174 848.6376 60
CGGTC 2935 53.073494 96.30739 21
CCGCT 50 52.95753 766.5934 54
CACCG 40 52.09122 496.09683 70
TCACC 110 51.185135 663.4551 30
TACCC 110 51.185135 506.20425 56
ATCCC 110 51.185135 503.45596 50
CCTCT 135 51.09026 536.7733 15
CTCCT 130 49.19803 411.69818 56
GTCGC 2715 49.09524 96.45932 24
GGCGC 960 48.584095 144.58463 34
CGCCT 45 47.66177 751.1284 15
CGTTC 7325 47.32862 80.15483 8
ATCGC 5950 47.269398 81.656105 4
CGAGC 2110 46.91361 86.723274 15
CCGCA 35 45.579823 953.61115 62
CGACC 35 45.57982 1880.1486 51
ACCGC 35 45.57982 941.6704 53
TCGTC 7050 45.551777 73.28423 5
TGCGC 2465 44.5745 105.208954 58
CATCC 95 44.20535 501.40182 44
CGTAC 5310 42.18496 67.60844 15
CCTCA 90 41.878754 505.18423 54
ACGAC 4205 41.07485 69.52563 26
TCGAC 5115 40.63579 75.110306 58
CGCCA 30 39.068413 464.63684 32
ACGGC 1720 38.24238 87.03904 26
CCTTC 100 37.84464 409.1517 49
GACGC 1680 37.353016 86.718994 17
CCATC 80 37.22556 496.42746 24
CGATC 4625 36.74302 137.97101 10
AACGC 3745 36.58152 76.16877 5
AGCGC 1630 36.24132 89.54593 62
GCGAC 1620 36.018982 87.89762 45
ACGTC 4370 34.717186 126.76582 15
TCCTC 90 34.060173 273.41693 52
CCCGA 25 32.55701 466.8523 41
TCTCC 85 32.167942 288.3343 70
TACGC 4005 31.817469 51.146397 37
CCCGG 10 29.642242 1098.8783 66
CGTGC 1630 29.475227 71.14456 37
TCCAC 60 27.919165 333.44513 40
CGAAC 2840 27.741392 48.616344 23
CCCTG 25 26.478764 390.97974 65
TCCCG 25 26.478764 390.97974 65
CCCGT 25 26.478764 382.93237 53
GACCC 20 26.04561 470.03714 51
CGGGG 18555 16.032295 21.401512 43
CCTCG 15 15.887259 766.59326 54
ACCCG 10 13.022805 475.01306 60
GGGCG 14460 12.494045 17.232601 56
GGCGG 14310 12.364438 16.31644 57
GCGGG 13570 11.725047 17.048159 42
GACGG 28320 10.750323 14.123962 52
CCTGC 10 10.591506 382.28333 51
TCGGG 31965 9.868614 12.057934 21
GCACA 930 9.08433 128.0974 11
GGACG 23090 8.765006 11.363064 50
CGTCT 1340 8.65807 208.40063 1
CGAGG 22565 8.565715 11.037449 15
ACACG 875 8.547085 128.15579 13
AGCAC 870 8.498243 124.63531 10
GCGAG 21795 8.273421 10.855916 36
GGTCG 26600 8.212268 10.289978 17
GTCGG 25690 7.9313216 10.194435 21
GGCGA 20580 7.8122053 10.267078 26
CGGGA 20015 7.5977306 9.706141 48
ATCGG 55720 7.557637 9.759158 2
TTCGG 67860 7.485864 8.484637 8
CGGTA 55045 7.466083 9.326487 1
ACGGA 44630 7.443012 9.22524 53
TCGGT 67405 7.435672 8.687117 52
CGGAG 19530 7.413624 9.956447 6
AGCGG 18855 7.1573925 10.664456 24
CGGGT 23145 7.145599 9.592015 34
CACGT 880 6.991104 98.595634 14
GCGGA 18335 6.9599996 10.398364 25
TTTCG 176080 6.940405 7.6417274 3
GCCGT 375 6.781111 78.14672 48
GAGCG 17755 6.7398305 9.417897 4
CGGAA 39790 6.6358376 8.62983 4
GCGGT 21405 6.6084056 9.41075 5
ACGGG 17400 6.6050715 8.80687 33
GGGGG 445030 6.565018 7.921377 2
TCGGA 46945 6.3674316 8.845497 3
CGAGA 37860 6.3139687 8.035918 33
TCGAG 45970 6.2351866 7.5980673 62
AGGCG 16265 6.1742234 8.339079 48
GTTCG 55945 6.1714807 7.3115773 1
GGCGT 19795 6.111347 8.535002 4
CGGAT 44135 5.9862943 7.6151905 47
CGGTT 53845 5.9398227 7.059367 53
TCTGC 915 5.9120398 222.14287 6
TGGCG 19015 5.8705363 8.023088 51
TACGG 42680 5.788944 6.970606 4
TGCCG 320 5.7865477 78.096596 47
TCGTT 145690 5.7425466 6.6079288 3
GACGA 34410 5.738607 7.356809 25
TTCGT 144035 5.6773133 6.366147 25
CTGCT 860 5.556671 219.85327 7
TATCG 114120 5.5307417 6.35742 61
AGACG 33080 5.516801 6.9389057 24
GTCGA 40620 5.509534 7.0218954 13
GCGAA 32915 5.4892836 7.4782043 26
CGTTT 139020 5.4796405 6.3566394 1
CGAAA 74625 5.4676366 6.547394 27
AGCGA 32355 5.3958917 7.034192 9
GTCGT 48860 5.3899107 7.0769815 2
AACGG 32255 5.3792143 6.7050257 43
ACGGT 39355 5.3379545 7.0186796 4
GGCAC 240 5.3361454 24.115822 53
CGAAG 31955 5.329183 6.590589 27
GACGT 39250 5.3237123 6.5382166 7
GATCG 39145 5.3094707 8.076545 1
GTCAC 665 5.28305 42.4407 28
GTACG 38505 5.222664 6.4898744 4
CGGTG 16900 5.2175684 7.5504856 5
GCGAT 38380 5.2057095 7.115118 7
TTCGA 106530 5.162898 5.84258 24
CGTGG 16640 5.137298 6.893874 2
AAGCG 30525 5.0906997 6.662328 47
GCCGG 100 5.0608435 36.047993 28
TGACG 37105 5.0327735 6.475707 43
TAGCG 37000 5.018532 6.348563 14
AAACG 67580 4.9514627 5.7391686 2
ACGAG 29145 4.8605556 6.1079655 56
GCGTT 44060 4.8604064 6.099555 7
AGTCG 35675 4.838814 5.8676114 12
CGTTA 98790 4.787784 5.548409 6
GCACG 215 4.780297 24.138762 54
CGTAG 35150 4.767605 6.3904114 33
GCGTA 35150 4.767605 6.321094 25
TTGCG 42630 4.702658 5.684358 66
CGAGT 34650 4.699787 5.7209396 8
GCGTG 15130 4.671113 7.33138 3
TTACG 96180 4.661293 5.513865 3
TGCGG 15095 4.6603074 6.256056 23
ATTCG 96065 4.6557198 5.513444 63
TCGAT 95700 4.63803 5.256401 8
CGATA 77710 4.6306863 5.5815187 28
TGTCG 41650 4.594551 5.9821997 1
ATCGT 94485 4.579146 5.2563877 5
GCGCA 205 4.557957 31.52129 4
GGGCC 90 4.5547595 35.99451 24
GGCCG 90 4.554759 54.01199 25
ACGAA 61970 4.5404286 5.214952 26
ACGTT 93660 4.5391636 5.507626 46
TCGTG 41030 4.526157 6.255938 6
TCGTA 93155 4.514688 5.157007 26
CGATT 91190 4.4194565 5.0905447 41
AGCGT 32425 4.397997 5.432475 6
ATCGA 73535 4.381901 5.1413064 21
AATCG 73505 4.380113 5.247905 51
TCGAA 73375 4.3723664 5.133738 36
CGTTG 39280 4.3331084 5.276098 33
CGTAA 72260 4.3059244 4.9431505 27
ACACA 995 4.269987 21.721806 53
GAACG 25580 4.2660146 5.5378003 51
AACGA 58000 4.249553 4.976565 65
CGTGT 38305 4.225553 5.9431562 7
CACGG 190 4.224448 39.64655 31
TACGA 70860 4.2224994 5.1375766 19
TACGT 86050 4.17035 5.1187906 3
TCCGA 520 4.131107 107.00205 8
ATACG 68650 4.090807 4.810361 24
GTGCG 13235 4.0860662 6.353207 19
ACGAT 68050 4.055054 4.729098 26
CCGAT 510 4.0516624 104.18622 9
CGTAT 83280 4.036104 4.599389 66
GGGGC 4660 4.0264354 6.431311 8
ACGTA 67555 4.025557 4.8365045 4
CGATG 29460 3.9958363 5.0238476 28
TAACG 67050 3.995464 4.794264 4
TGCGA 29375 3.984307 5.163031 25
AACGT 65775 3.9194877 4.5849977 52
TTCCG 600 3.876747 87.025246 7
TCTCG 590 3.8121345 50.930984 40
TGCGT 34105 3.762237 4.788434 42
ACGCT 460 3.654441 101.36944 1
AACAC 850 3.6477275 13.755429 28
CGTGA 26035 3.531283 4.6151686 1
CGAAT 59245 3.5303695 4.1227703 31
ACGTG 25930 3.5170414 4.730489 26
TCACA 990 3.455348 22.384851 29
ACCGG 155 3.4462605 23.747458 27
GCTCG 190 3.435763 27.17659 68
ATGCG 25260 3.426165 4.593026 30
TCTTC 1465 3.3822098 81.8262 3
ACACT 960 3.3506408 18.67097 31
CTCGT 515 3.327541 48.641895 41
CCGGG 65 3.2895486 36.240112 39
CACGA 335 3.2723122 17.690378 55
GACAC 335 3.2723122 17.628185 51
GCTCT 500 3.230623 77.86183 3
GACCG 145 3.223921 24.095098 52
CACTG 405 3.2174969 17.265196 55
CCAGT 405 3.2174969 45.21985 25
ATGCC 400 3.1777747 34.284676 46
TCACG 395 3.1380522 14.375084 54
CACTT 1105 3.136693 15.205833 34
CCGGA 140 3.1127517 16.826384 69
CACAA 725 3.111297 15.5303335 54
CTCGG 170 3.0741034 32.189854 27
TCCAG 385 3.0586078 36.727364 24
CACAT 865 3.019067 17.69872 55
CAGTC 380 3.0188859 42.409126 26
TGCAC 380 3.0188859 14.361424 53
TACAC 860 3.0016153 13.868846 52
CGCAG 135 3.0015817 16.939802 70
GTCCG 165 2.9836888 19.362917 33
GCACT 370 2.9394414 25.875153 54
TCGCT 435 2.810642 11.69132 54
GAGCC 125 2.7792423 17.053625 71
ACAAC 645 2.7679815 19.943209 36
CAACG 280 2.7350671 10.560633 49
CTTCT 1175 2.712694 76.91629 4
TCGCA 340 2.7011085 14.239937 41
CGCAA 270 2.6373858 13.848867 5
AACCA 610 2.617781 13.722904 22
CAAAC 605 2.5963237 16.799683 26
CGACA 265 2.5885456 10.538696 46
CAACA 600 2.5748665 18.41892 37
AGCCG 115 2.556903 16.106588 55
TTCAC 900 2.5547726 12.29472 51
AAACC 590 2.531952 12.502777 59
CCGGT 140 2.531615 19.565004 50
TCACT 880 2.498 13.354586 54
ACCAA 580 2.4890375 12.5446415 61
ATCAC 710 2.478078 9.971392 34
CCAAA 575 2.4675803 13.694655 17
GGACC 110 2.4457333 15.819489 25
CACAG 250 2.4420242 24.721363 53
TCCGG 135 2.4411998 13.480638 67
GCGCT 135 2.4411998 19.632162 54
CGTCA 305 2.423053 11.359816 36
AACTC 675 2.3559191 18.601498 22
CGGCT 130 2.350785 19.235168 17
CGCTG 130 2.3507848 19.649338 55
CCAGG 105 2.3345635 23.656506 18
CTCTT 985 2.2740455 30.275562 4
CGCTA 285 2.2641642 20.142727 55
GAACC 230 2.2466621 17.335024 20
TGCTC 345 2.2291298 14.0162525 53
CGGCA 100 2.223394 16.82638 69
GGCCA 100 2.223394 24.056183 50
ACGCA 225 2.1978219 10.59487 53
CGCTT 340 2.1968234 11.70155 55
CGCAT 275 2.18472 11.500068 54
ATCTC 765 2.1715567 23.376215 39
TGGCC 120 2.1699555 13.114536 56
CAGCG 95 2.1122246 16.025265 49
GACCA 215 2.1001406 14.25184 60
TTGCC 325 2.0999048 9.41216 59
GGCTC 115 2.0795407 19.355303 32
CCGTT 320 2.0675986 9.41216 59
TGACC 260 2.0655534 11.572739 59
CAGAC 205 2.0024598 10.59487 53
GCCGA 90 2.0010545 15.808502 23
CCGTG 110 1.9891258 19.423498 39
CTGCG 110 1.9891258 12.974171 42
CACTA 560 1.9545403 12.641941 55
CTCGA 240 1.9066646 8.642466 56
TCCGT 295 1.9060673 7.1554017 65
CTTCG 295 1.9060673 16.185553 38
AACCG 195 1.904779 10.641886 57
GCAAC 195 1.9047787 10.560632 49
ACCGA 195 1.9047787 10.576911 51
GGTCC 105 1.898711 19.413513 38
CCGAG 85 1.8898848 15.871807 33
GCCAG 85 1.8898848 16.248423 61
AGGCC 85 1.8898848 24.021362 48
ACTCG 235 1.8669425 11.337889 32
CTACA 525 1.8323815 6.1880407 12
GACTC 230 1.8272204 14.166157 31
TTTCC 770 1.77768 5.78328 38
CAGCA 180 1.7582575 10.604947 54
AGCCA 180 1.7582573 17.318348 15
GCTCA 220 1.747776 17.250101 54
ACCTG 220 1.747776 14.1779375 33
ACCGT 220 1.747776 8.666574 58
ACTGC 220 1.747776 11.391951 41
TGCCT 270 1.7445364 7.070311 60
CTCTG 270 1.7445362 14.04186 55
GGCCT 95 1.7178814 12.928489 36
TCAAC 490 1.7102227 10.004371 40
GTACC 215 1.7080538 8.519861 36
CTCAG 215 1.7080537 8.625051 54
TACCG 215 1.7080537 8.595544 50
TTCTC 735 1.6968766 10.841698 52
TCTCT 730 1.6853333 13.367808 54
ACAGC 170 1.6605763 10.604947 54
ACCAT 475 1.6578692 7.455682 27
ACTCA 470 1.6404177 7.5713315 53
CCTGG 90 1.6274666 13.241474 62
CAACT 460 1.6055154 7.4900956 37
TACCA 460 1.6055152 8.733791 36
ACTCT 560 1.5896363 10.2727585 54
TACTC 555 1.5754431 9.228759 52
GCCAA 160 1.5628954 17.32186 16
AGACC 160 1.5628954 10.525545 44
CAGGC 70 1.5563759 16.707514 68
TCAGC 195 1.5491651 11.452012 49
CCATG 195 1.5491651 8.632598 55
CTGGC 85 1.5370518 13.075671 53
GTGCC 85 1.5370518 6.636043 63
GCTGC 85 1.5370518 13.033418 49
CATCA 440 1.5357102 9.986793 37
CTTTC 665 1.5352693 7.45875 43
ACTTC 535 1.5186706 10.15288 37
CTCAT 535 1.5186704 10.2727585 54
GTCTC 235 1.5183928 13.992495 51
CCTTG 235 1.5183928 9.41216 59
GCTAC 190 1.5094429 11.270355 16
TCTCA 530 1.5044773 13.341895 53
CTCAA 430 1.5008076 12.641941 55
ATACC 430 1.5008076 7.435439 21
CCTGT 230 1.4860865 6.945135 40
CTGTC 230 1.4860864 11.530978 33
CATAC 425 1.4833566 10.086435 52
TAACC 425 1.4833565 6.345849 58
CCTTT 640 1.4775524 5.797503 42
GATCC 185 1.4697207 14.465923 59
CCGAA 150 1.4652144 7.0571766 52
TTACC 515 1.4618979 5.1861444 61
GCAGC 65 1.445206 16.248423 61
CTGCA 180 1.4299985 8.583102 48
CTGAC 180 1.4299985 8.666574 58
GCCAT 180 1.4299985 8.468069 22
GACCT 180 1.4299985 8.655092 57
TGCCA 180 1.4299985 11.268072 13
CTACG 180 1.4299984 11.371987 38
ACCAG 145 1.4163741 10.641886 57
ACTAC 400 1.3961004 6.2938404 50
CTATC 490 1.3909317 12.158246 33
CCATT 490 1.3909317 6.1297426 47
CCGTA 175 1.3902764 8.506762 33
CATGC 175 1.3902764 8.5067625 33
ATCCG 175 1.3902763 5.8902206 66
CCAAT 390 1.3611978 7.5174146 43
GCCTT 210 1.3568615 6.990794 50
GCATC 170 1.3505542 11.407322 43
AGTCC 170 1.3505542 11.444137 48
CCATA 385 1.3437464 6.215195 28
AGATC 22365 1.3327153 39.472843 72
AACCT 380 1.3262954 5.092343 60
CTTGC 205 1.3245554 11.53098 33
TGTCC 205 1.3245554 7.0591197 59
TACCT 465 1.3199658 6.2358804 62
ACCTT 465 1.3199658 6.2502966 63
CGACT 165 1.310832 5.846668 64
CCTAG 165 1.310832 8.472834 23
ACATC 375 1.3088439 5.0083723 42
TCATC 460 1.3057727 6.0884843 36
TTCCA 455 1.2915795 5.054846 28
CCTGA 160 1.2711098 11.348338 34
AGCTC 160 1.2711098 8.883728 67
CTCTA 445 1.2631931 11.309921 55
TCCTG 195 1.2599428 7.059119 59
AAGCC 125 1.221012 7.069965 54
CTTAC 430 1.2206136 10.131872 33
GTCCA 150 1.1916654 8.693314 60
GCCTG 65 1.1753924 12.935373 37
CCAGA 120 1.1721715 6.955356 27
TCCTT 500 1.1543378 5.061497 61
CATCG 145 1.1519433 5.7616444 56
CAGCT 145 1.1519433 8.72612 62
GCCTA 145 1.1519432 8.472833 23
CAATC 330 1.1517826 6.2288313 33
CATCT 405 1.1496477 5.091665 42
AATCC 325 1.1343313 5.0842824 59
GTCCT 175 1.130718 6.918588 33
GTTCC 175 1.130718 6.9854794 49
CTTCA 390 1.1070682 6.056815 24
ATCCT 390 1.1070682 7.055155 21
TCCAA 315 1.0994289 6.1939993 20
ATTCC 385 1.0928751 5.1542697 57
TCCAT 380 1.0786818 5.186144 61
CAAGC 110 1.0744905 10.441342 29
TTCCT 465 1.0735341 5.0526032 60
TAGCC 130 1.0327767 11.268072 14
CATTC 355 1.007716 8.105498 33
GCTTC 155 1.001493 6.9010777 27
ATCCA 255 0.8900139 6.365428 60
CCAAG 90 0.87912875 10.390498 17
CTAGC 90 0.71499926 8.511253 34
Produced by FastQC (version 0.10.1)