FastQCFastQC Report
Wed 2 Apr 2014
mcf_M3_R2.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename mcf_M3_R2.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 7867124
Filtered Sequences 0
Sequence length 19-76
%GC 16

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 105 951362.8 1.4844629E7 8
GGTGG 375 26038.69 945202.6 35
GAGGG 145 8474.737 230769.89 22
AGGGG 120 7013.576 95944.266 19
GGGGA 100 5844.647 115275.29 21
CGGGG 35 5729.0234 109110.9 39
GGGCG 30 4910.5913 108990.65 38
GGAGG 80 4675.7173 57476.375 5
GGGGT 60 4166.191 91005.23 9
GGGAG 60 3506.788 40547.723 53
GGCGG 15 2455.2957 56779.39 53
GGGTG 35 2430.2776 45513.434 11
GTGGG 35 2430.2776 82359.23 68
GCGCG 675 1996.0529 7751.768 8
TGGGG 25 1735.9126 45519.742 3
GGGGC 10 1636.8639 54638.777 42
GCGGG 10 1636.8638 57492.926 54
GAGCG 1055 1113.9519 17305.754 4
CGGTG 355 445.31973 17451.389 34
GGTCG 355 445.31967 15034.631 38
GGAAG 1090 410.9473 2842.4795 5
GTAGG 770 344.88956 6752.1934 13
TAGGG 750 335.93137 6752.2188 14
CGCCG 6160 329.08243 1104.23 41
GGGAA 790 297.84253 5563.051 16
CCGCG 5530 295.42627 466.59506 45
CGCGA 14090 268.76993 369.4983 19
AGGGA 665 250.71556 5437.1924 15
TCGCG 10325 233.98572 341.69534 3
CGCGC 4245 226.7784 350.14804 13
GAGTG 495 221.71468 4863.432 22
CGTCG 9765 221.29497 556.94696 7
CGACG 11085 211.44887 339.37927 68
GTGGT 380 202.20993 6540.208 36
ACGCG 9540 181.97765 250.01866 6
GCGTG 145 181.89116 2231.8203 68
TCGGG 135 169.34694 2466.5742 14
GTCGG 135 169.34694 2877.6592 13
CGCGT 7365 166.90604 281.223 44
GTCCG 6475 146.7368 232.70091 34
CGGCG 45 133.07019 2906.985 7
GTGTG 225 119.72957 727.67084 70
GGCGC 40 118.284615 1971.169 39
GGACG 105 110.86725 1384.471 5
CGCGG 35 103.49904 995.21246 46
CCGGG 35 103.49904 1938.2179 12
GCGGC 35 103.49904 2907.2898 10
GGTAG 205 91.82123 1623.8135 25
CGGGA 85 89.74968 1038.3531 5
CGTGG 70 87.809525 1233.3013 12
CGGGT 70 87.80952 2055.7764 15
CGGAG 80 84.47028 721.9383 70
GGCGT 65 81.53741 1233.1256 8
GCCGC 1505 80.40082 263.42484 60
GTGCG 60 75.2653 1119.9012 62
GGCCG 25 73.92789 2487.0825 71
AGCGG 70 73.9115 1038.3531 5
GAGAG 190 71.63302 500.94565 36
GGGTA 155 69.42581 1622.9854 24
GCGGA 65 68.6321 721.93823 70
GCCCG 1215 64.9083 164.05942 51
GGTGT 115 61.19511 1046.4838 15
GCCGG 20 59.142307 1938.2179 12
TGGTG 110 58.534454 871.94336 14
AGGGT 125 55.988564 1622.212 23
GTGAG 125 55.988556 446.13235 35
GATCG 6880 55.671566 333.47476 71
GCCGA 2915 55.604282 139.90596 41
GCGAA 7830 53.330685 79.66292 43
GACGC 2740 52.266117 109.736015 60
GACCG 2675 51.026226 101.74979 41
AGAGG 135 50.897144 1487.1941 21
GGGTC 40 50.17687 1233.2855 10
GCGTC 2180 49.40328 393.5663 6
GCCGT 2005 45.43742 347.97296 42
GCTCG 1940 43.964386 99.11299 72
GTGGC 35 43.90476 1693.7036 47
TGGCG 35 43.904755 837.4559 42
GTCGT 4455 42.82746 170.10257 8
GCGCC 780 41.66953 105.969406 31
GCGAC 2090 39.86722 104.141624 51
GTCGC 1730 39.205357 317.22995 39
GAACG 5745 39.1296 73.64984 8
GCGAT 4835 39.12384 65.19159 58
GACGA 5550 37.80144 78.36549 60
AGGCG 35 36.95575 540.14716 65
GGAGA 95 35.81651 403.1248 66
GTACG 4285 34.67335 63.52889 67
GTCGA 4235 34.268764 66.50074 60
GTGGA 75 33.593132 376.71347 71
GCGCA 1690 32.23713 88.89136 63
AAGGG 85 32.04635 495.11993 19
GCGCT 1410 31.953497 77.3275 51
TGTGG 60 31.927885 363.83542 70
CGAGG 30 31.676357 418.42227 59
GGAGC 30 31.676357 888.0469 71
GCGAG 30 31.676357 362.715 52
GCGTT 3095 29.75331 59.87397 5
CGGGC 10 29.571154 1056.1117 55
GGGCC 10 29.571154 970.8653 19
TTGGG 55 29.267227 523.8639 18
GAGGA 75 28.276192 385.93628 51
GACGT 3410 27.593029 57.35855 47
GCGTA 3405 27.55257 57.083042 45
AGGTG 60 26.874506 265.634 68
GCACG 1405 26.800692 100.11109 72
GGGTT 50 26.606571 348.86606 5
GGCGA 25 26.396963 704.90784 42
GGCAG 25 26.396963 923.58154 72
GGTGC 20 25.088434 424.41663 49
GCGGT 20 25.088434 1097.248 72
AGTGG 55 24.634968 452.22864 46
TGGAG 55 24.634964 440.3671 12
GAAGG 65 24.506033 371.15836 18
GTTCG 2480 23.841099 44.68439 35
TGGGA 50 22.395422 294.11014 19
GACGG 20 21.11757 351.0352 37
GAGGC 20 21.11757 360.28705 51
AGGGC 20 21.11757 451.6147 61
ACGGG 20 21.117569 346.66077 19
AGGAG 55 20.735874 257.77795 70
GGAGT 45 20.155882 376.71356 71
GATGG 45 20.155882 440.49402 1
GGCTG 15 18.816328 417.04248 37
GCTGG 15 18.816328 641.7143 65
TGGGC 15 18.816326 834.08484 37
GTTGG 35 18.6246 523.68286 17
GAGGT 40 17.91634 391.7875 72
CCCCG 17185 16.585524 23.13094 19
ATCGG 1995 16.143137 237.93457 66
GGTTG 30 15.963943 177.6263 42
GGGCA 15 15.838179 358.45508 50
CAGGG 15 15.838177 351.91644 39
ATGGG 35 15.676798 440.51196 2
TGAGG 35 15.676798 456.17487 50
GGATG 35 15.676796 297.8214 37
TCGGA 1870 15.131661 217.81334 67
GGTGA 30 13.437253 294.11014 19
TGGGT 25 13.303286 177.15997 38
CCGCC 13455 12.9856415 18.267517 65
CCCGC 13270 12.807094 18.02824 10
TGCGG 10 12.544218 584.5659 63
CGCCC 12275 11.846805 17.232405 62
CGGAA 1710 11.646931 141.3767 68
CTCGG 500 11.331027 352.34534 32
CGTGT 1165 11.199549 132.31917 10
GGGAT 25 11.197713 149.32523 41
CCGTC 26865 10.998713 14.793792 20
GCAGG 10 10.558785 433.87582 60
GGGAC 10 10.558785 694.8381 22
TCGTG 1090 10.478548 135.44455 9
CCCGA 28685 9.88508 12.534034 8
AGCGT 1155 9.346026 127.31985 5
AGAGC 1360 9.263057 113.85904 3
CCCCC 502630 8.763625 10.602032 18
CGTCC 21120 8.64667 11.22482 30
CCTCG 20485 8.386697 10.609458 46
CGACC 24005 8.272315 12.358906 49
CCGAC 23555 8.117243 10.388569 8
CTCGC 19345 7.919974 10.935026 60
CTCCG 19245 7.879033 11.607951 60
ACCGA 62840 7.732275 9.821953 44
TACCG 52425 7.663711 9.583789 4
CCGAA 60950 7.4997163 9.262109 47
TCCGT 43145 7.4930873 9.226246 63
CCGAT 50805 7.426893 10.003293 42
TCCCG 18130 7.4225445 10.196103 46
TCGCC 18045 7.387745 11.999942 40
ACCCG 21215 7.3108587 9.2024555 42
CCCGG 130 6.9449215 70.020744 6
CGAAA 157635 6.925792 8.217448 62
TCCGC 16820 6.886222 8.925204 44
CCGCT 16475 6.7449765 9.139588 48
ACCGC 19155 6.6009665 9.828603 2
CGCTC 16055 6.5730257 9.641286 71
TCTCG 36770 6.3859262 9.531114 31
CGCCT 15495 6.3437576 10.7077875 59
CCCGT 15455 6.327381 9.4401045 43
TTCCG 36430 6.326878 7.969358 56
CTCGA 43185 6.3129687 7.79508 57
CTGGG 5 6.272109 670.6462 66
GGGCT 5 6.2721086 456.84207 56
TCCGA 41835 6.11562 8.09242 47
ACGCC 17590 6.061655 9.518865 58
GTGTA 1745 5.989847 47.24886 11
CACCG 17095 5.8910737 7.9459763 43
ATCCG 40250 5.8839173 7.672081 72
CGAAC 46980 5.780749 7.534083 72
AACCG 46360 5.7044597 7.2252407 17
CCGTA 38990 5.6997256 7.5651894 48
TGTAG 1650 5.6637526 66.65261 59
TCGTC 32445 5.6347947 7.7475038 72
CGATA 107340 5.602837 7.07684 72
AACGA 127070 5.5828996 6.5278525 44
ACGAA 125800 5.527101 6.5879307 33
CGTCT 31780 5.519303 8.203239 72
TCGAC 37730 5.515533 7.2539515 71
CTTCG 31680 5.501935 7.2103715 56
CGCCA 15910 5.482714 7.5920267 42
TTTCG 73625 5.424141 6.685624 59
TCGCT 30905 5.3673396 7.728863 58
ACGAC 43575 5.361773 6.6437254 39
ACGTC 36565 5.3452277 6.7742205 41
CGTAC 36435 5.3262243 7.1901565 66
CCGTT 30665 5.3256583 6.8142176 48
ACCGT 36350 5.313799 6.7121525 30
CCACG 15395 5.30524 7.911681 69
TTCGC 30535 5.303081 7.542997 22
CGATC 36255 5.2999115 7.0080547 71
AAGAG 2170 5.27741 39.863758 2
AAACG 119825 5.264586 6.2751026 38
CCGCA 14930 5.144998 7.6809735 3
ATCGC 35050 5.1237593 6.541326 57
CGTCA 35005 5.1171813 6.7050314 28
TCGAA 97295 5.0785174 5.9704523 71
TCGGT 520 4.9989405 136.82869 33
CGTTT 67675 4.985789 6.060997 40
CGACT 34030 4.9746513 6.521269 72
CGCTA 33645 4.9183707 6.7777786 59
CTCGT 28270 4.9097133 6.5918097 68
ACTCG 33055 4.8321214 6.018324 35
CGCTT 27520 4.779459 6.412246 70
ATCGA 90945 4.7470655 6.2093563 72
CTACG 32340 4.7276 6.393313 71
CACGC 13660 4.7073455 6.3721995 60
AACGC 38230 4.7040877 6.1024833 57
TAACG 89440 4.6685085 5.7207117 66
CACGA 37760 4.6462555 5.973317 70
CGACA 37595 4.6259527 6.3501554 72
TATCG 74200 4.6012936 5.6849213 71
CGAAT 87085 4.5455847 5.545298 49
CGTAA 86995 4.5408874 5.498758 62
TACGC 30990 4.530251 6.7593703 57
TTCGT 61445 4.526809 5.948381 71
TACGA 86305 4.5048714 5.196866 57
CGCAA 36605 4.5041366 6.4852033 59
GAAGA 1850 4.499174 46.968903 60
ACGAT 85965 4.4871235 5.674526 70
AACGT 85490 4.4623303 5.3671985 48
CAACG 36130 4.4456887 5.65446 31
TCGAT 71655 4.443473 5.0383806 68
AATCG 84570 4.414309 5.2530828 1
TTCGA 70580 4.37681 5.3993325 27
ACACG 35365 4.3515577 5.4917417 31
ACGCT 29190 4.2671194 5.66434 58
CGATT 68755 4.263638 5.406292 59
CGCAC 12305 4.2404017 5.7975554 67
TCGTA 67830 4.206277 5.044524 72
TTACG 67695 4.1979055 5.171708 56
TCGTT 56930 4.1941776 5.047892 33
ACGTA 80150 4.1835976 4.85655 49
CATCG 28610 4.1823325 6.137665 72
CGTTC 23945 4.1585813 5.6966147 71
ATACG 79550 4.1522794 5.315943 59
CGTAT 66020 4.094035 4.899542 62
GCCCC 4185 4.0390124 8.9949665 63
ATCGT 64990 4.030163 4.8412714 32
TACGT 64600 4.005978 4.994403 60
TCGCA 26645 3.8950806 5.8148394 61
ACGTT 61550 3.8168406 4.8852525 33
CGTTA 60535 3.753899 4.507931 46
TCACG 25475 3.724045 5.220711 69
CACGT 25360 3.7072334 4.597742 70
ACGCA 29205 3.5935884 5.098262 58
ATTCG 56570 3.5080214 4.339376 72
CCGGA 180 3.4335406 33.37036 72
AGTGT 995 3.4154146 39.55615 23
TGGTC 355 3.4127378 118.25322 37
CGCAT 23315 3.408287 5.3508253 62
TGTCG 350 3.364671 64.7626 59
CGGAC 175 3.3381643 31.26412 4
GTGTC 340 3.2685375 21.00624 56
GTAGA 1100 3.178216 52.239002 60
AGCGC 165 3.147412 32.378777 50
CGTAG 385 3.1153421 38.47929 59
GGAAA 1270 3.0886223 37.49619 17
CCGTG 135 3.0593774 23.0022 49
CGTTG 315 3.0282042 38.09565 59
CGCTG 130 2.946067 71.84393 59
TGTGT 700 2.8546176 19.057518 42
CGTGC 125 2.8327568 30.773726 50
CCGGT 125 2.8327568 22.285742 4
TGCCG 125 2.8327568 71.84393 59
ACCGG 145 2.7659078 32.086445 71
TCGGC 120 2.7194467 30.989557 70
TGCGC 120 2.7194467 26.879654 68
CGTGA 330 2.6702933 13.735225 50
ACGTG 330 2.6702929 32.852997 49
CTGCG 115 2.6061363 23.0022 49
GTGCC 115 2.6061363 38.66375 51
GAAAG 1070 2.602225 44.67264 72
CCGAG 135 2.5751557 25.75512 47
AGCCG 135 2.5751555 22.67732 59
GTTGT 630 2.569156 53.62303 60
TGACG 315 2.5489163 38.47929 59
TGCGA 315 2.5489163 21.97636 50
AGAGT 865 2.4992332 31.342335 21
GCTGT 255 2.4514034 59.253956 60
TGTTG 585 2.385645 66.25742 59
CAGCG 125 2.3844032 19.361534 49
CGAGA 350 2.383875 17.873018 72
GTGTT 580 2.3652546 20.872566 51
TTGCG 245 2.3552697 15.9801655 37
ACGAG 345 2.3498194 17.185356 71
AGTCG 290 2.3466213 44.892506 59
CGAGC 120 2.289027 25.678944 46
CGAAG 335 2.2817087 35.088074 59
TGCGT 235 2.2591364 12.802104 38
CGAGT 275 2.2252443 13.735225 50
TTGTG 545 2.222524 19.316427 49
GTCTG 230 2.2110698 12.979814 47
TCGAG 270 2.1847854 13.656797 47
TAGTG 620 2.128198 18.581858 49
CGGTT 220 2.1149364 12.601825 10
GTGCT 220 2.114936 26.242138 51
AGACG 310 2.1114318 37.78716 59
ATGCG 260 2.1038673 16.4265 49
AGCGA 305 2.0773766 16.185802 50
AAGCG 305 2.0773766 18.435469 49
GAGTC 255 2.0634084 22.088675 51
GTGAC 255 2.0634084 22.088675 51
CAGTG 255 2.0634081 24.63975 49
TAGCG 250 2.0229495 10.951 49
CGATG 250 2.0229495 28.859468 59
CGGCA 105 2.0028987 19.072891 39
AGTGC 245 1.9824904 24.723404 50
GAGCA 285 1.9411552 13.970847 70
GATGT 565 1.9394063 52.188347 60
GCTGC 85 1.9262747 65.18517 60
CTGTG 200 1.9226694 12.773372 36
GGTTT 470 1.9166719 8.017143 9
TGCTG 195 1.8746027 49.524345 59
GTTGC 195 1.8746024 43.4529 60
TGTGC 195 1.8746024 11.064279 58
GTAGT 545 1.8707547 38.08339 60
CGGCC 35 1.8697865 36.52667 70
GACAG 270 1.8389891 13.753529 46
TTCGG 190 1.8265359 12.979814 47
CGCAG 95 1.8121465 37.795536 59
GAAGC 260 1.7708784 25.18891 60
AGATG 610 1.7624651 73.290886 72
ATGTG 510 1.7506145 15.09776 49
GTGAT 510 1.7506144 15.2264385 51
TACGG 215 1.7397364 10.610898 2
TGTGA 505 1.7334515 18.644943 50
GTAGC 210 1.6992775 33.25037 60
TGAGT 495 1.6991259 11.6530905 50
AGTGA 580 1.6757866 16.674793 50
ACGGA 245 1.6687121 8.9301195 3
AGAAG 680 1.6537505 38.549778 59
GTTGA 480 1.647637 38.083385 60
GAGTT 480 1.647637 16.397703 51
CACGG 85 1.6213942 18.829144 22
GAGCC 85 1.6213942 32.54426 51
ACGGC 85 1.621394 18.75741 3
CGGTA 200 1.6183596 8.21325 49
GGCCC 30 1.6026741 35.023846 5
CCGGC 30 1.6026741 35.021595 3
CGGTC 70 1.5863439 14.937694 25
TCCGG 70 1.5863439 14.984235 31
CGGCT 70 1.5863439 14.8571615 4
CAGAG 230 1.5665463 18.435469 49
GTGAA 540 1.560215 15.774127 51
AAGTG 535 1.5457685 17.59592 49
GCTTG 160 1.5381355 12.979814 47
GAGCT 190 1.5374415 13.805422 51
GTGCA 190 1.5374413 10.713689 33
GAGAC 225 1.532491 11.321972 37
TGATG 445 1.5274969 31.285923 59
GCAGC 80 1.526018 12.877558 47
TGAGC 185 1.4969826 10.98818 50
TGCAG 185 1.4969826 28.859468 59
GTTTG 365 1.4884793 10.979623 46
TTGGT 365 1.4884791 9.443203 32
AACGG 210 1.4303249 8.931461 2
AGTTG 415 1.4245197 25.844893 59
TTGAG 415 1.4245195 11.613661 49
ACGGT 175 1.4160644 10.656892 23
GAAGT 480 1.3868577 24.93225 60
CGGAT 170 1.3756056 8.210959 48
GATGA 465 1.3435186 24.932251 60
AGTAG 460 1.3290721 29.769043 59
GAGAT 460 1.329072 12.150177 71
TGAAG 450 1.3001792 18.475315 46
GACTG 160 1.2946876 13.616406 46
CTGAG 160 1.2946876 10.746281 35
GCTAG 160 1.2946876 10.925438 47
ATGAG 435 1.25684 11.558883 41
GCAGT 155 1.2542286 29.925333 60
GCCTG 55 1.246413 19.822598 72
GGTCC 55 1.246413 12.708593 67
GAGTA 425 1.227947 8.872947 51
GCAGA 180 1.2259928 30.786446 60
AGAGA 495 1.2038331 8.181689 71
TGGTT 290 1.1826273 9.448916 33
GTATG 335 1.1499134 10.397055 46
TGAGA 395 1.1412685 7.7155895 42
TAGAG 390 1.1268221 9.775513 49
GATAG 390 1.1268221 7.802155 47
GTTAG 320 1.0984247 10.397055 46
GAGAA 450 1.0943937 8.298427 51
AGCTG 135 1.0923927 16.033037 59
GATGC 135 1.0923927 19.950222 60
GGACC 55 1.0491374 12.951511 50
TTTGG 255 1.0398965 6.691111 18
GATTG 300 1.0297732 6.951931 47
GTCAG 120 0.9710157 5.5221686 51
GCTGA 120 0.9710157 10.760729 37
GCAAG 140 0.9535499 16.045784 46
GGATC 115 0.9305567 14.15586 72
CTTGG 95 0.91326797 9.534571 31
GCATG 110 0.89009774 24.509533 46
ATTGG 250 0.8581443 5.62626 1
GGCAA 125 0.85138386 6.8057833 40
AGCAG 125 0.85138386 21.59266 59
CATGG 105 0.84963876 8.063752 36
GTAAG 285 0.82344687 8.751465 46
ACAGG 120 0.8173285 6.8530903 44
GGTTC 85 0.8171344 6.3866854 36
GGTAC 100 0.8091798 16.48227 50
TGGCC 35 0.79317194 15.225517 45
CTGGC 35 0.79317194 11.059988 64
GGCCT 35 0.79317194 14.888848 20
GGTAT 230 0.7894928 5.68496 34
GGACA 115 0.78327316 7.282747 66
GGAAC 115 0.78327316 6.7682085 34
GCCAG 40 0.7630091 16.685183 72
GGCAC 40 0.763009 6.9469185 56
AGATC 14455 0.75450915 57.253918 72
CTGGA 90 0.72826177 8.210959 48
CAGGT 90 0.72826165 6.921959 61
GGCTT 75 0.721001 6.527177 50
TCTGG 75 0.721001 7.1694937 57
CAAGG 105 0.71516246 9.196218 47
CAGGA 100 0.681107 6.768208 34
GGCTC 30 0.67986166 10.115922 62
CCTGG 30 0.67986166 7.649502 47
CTGGT 70 0.6729343 7.376187 58
TGGCT 70 0.67293423 6.5050907 49
GGTCT 70 0.67293423 5.701235 68
CCAGG 35 0.6676329 12.500933 6
TATGG 190 0.6521897 6.95193 47
GAATG 225 0.65008956 10.696234 46
AGGTC 80 0.6473438 8.652166 66
ACTGG 80 0.6473438 8.194078 47
TTGGC 65 0.6248675 9.672653 44
TGGAA 215 0.6211967 7.5818157 72
AGGAC 90 0.6129964 6.8120923 41
GGTCA 75 0.6068847 5.6137505 53
TGGAT 170 0.5835381 5.7739396 71
AGGCC 30 0.57225674 12.505648 4
GGCTA 70 0.56642586 5.3039174 11
TGGAC 70 0.56642586 10.951 49
GGACT 70 0.56642586 10.893126 46
TAGGC 65 0.5259668 5.356845 33
GGCAT 65 0.5259668 10.951 49
ATGGC 65 0.5259668 5.4739733 48
TCAGG 65 0.5259668 5.462719 47
TGGCA 65 0.52596676 10.947945 48
TTGGA 150 0.51488656 7.0408916 70
GGCCA 25 0.47688064 6.419736 46
CAGGC 25 0.47688058 6.407851 45
AGGCT 45 0.36413088 5.310719 18
TAGAT 14675 0.32493752 12.046847 72
CAGAT 5760 0.30065528 10.13586 72
AAGAT 14920 0.27807435 9.977111 72
Produced by FastQC (version 0.10.1)