FastQCFastQC Report
Wed 2 Apr 2014
mcf_T1D5_R2.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename mcf_T1D5_R2.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6179274
Filtered Sequences 0
Sequence length 19-76
%GC 16

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 195 2198795.5 3.0408218E7 8
GGTGG 830 73449.6 4223352.5 35
GAGGG 265 18762.781 598566.6 22
GGGCG 55 11368.634 565344.0 38
CGGGG 45 9301.609 211056.3 34
GTGGG 105 9291.818 211227.17 36
AGGGG 125 8850.368 143206.4 7
GGGGT 90 7964.4146 149119.36 9
GGGTG 85 7521.9473 178970.89 11
GGGAG 95 6726.2783 71827.984 22
GGCGG 30 6201.073 70736.95 39
GGAGG 85 6018.249 119368.13 5
GGGGA 85 6018.249 119620.71 21
GGGGC 25 5167.5605 70537.26 37
TGGGG 40 3539.7402 89620.42 19
GCGCG 570 2159.8113 11064.572 56
GAGCG 1510 1959.8534 62143.363 4
GGTCG 835 1354.5443 72098.68 38
CGGTG 810 1313.9891 77297.64 34
GCGGG 5 1033.5122 69673.15 11
TAGGG 1145 636.2355 25284.584 14
GTAGG 1110 616.78723 25095.543 13
GTGGT 855 593.7979 31259.842 36
GAGTG 1020 566.77747 22924.16 22
GGGAA 1185 526.83014 20240.902 16
AGGGA 1090 484.59485 19934.484 15
CGGCG 115 435.7514 2554.593 8
GGAAG 880 391.2325 1948.7799 5
GGCGC 100 378.9142 9076.944 39
CGCCG 4860 337.57675 3448.8184 41
CCGCG 4270 296.5952 525.711 55
CGCGA 10605 252.32054 433.0969 6
TCGGG 150 243.33131 1655.3901 33
CGTCG 8015 238.34474 1593.8927 7
GCGGC 60 227.34854 3831.8362 1
GTCGG 140 227.10924 1642.2993 18
CGCGC 3265 226.78767 428.12918 41
TCGCG 7540 224.21951 344.6664 35
GTGTG 295 204.87764 1700.2197 51
CGACG 8325 198.07341 330.47952 48
GCGTG 120 194.66505 1527.2227 67
GTGCG 120 194.66505 1134.6743 51
ACGCG 7190 171.0688 320.89954 5
CGCGG 45 170.51141 3831.9824 3
CGCGT 5470 162.66322 260.66125 15
GGCCG 40 151.5657 2594.9978 41
TGGCG 90 145.9988 1640.5668 2
GGCGT 90 145.99878 1412.9602 64
GGCGA 110 142.77077 1750.072 6
TGGGC 85 137.88774 3875.0544 37
GCGAG 105 136.28119 925.4301 54
CGGGA 105 136.28117 1312.8878 4
GGTAG 235 130.58109 4328.008 25
CGTGG 80 129.7767 1648.0704 25
GTCCG 4310 128.16792 238.027 71
GAGAG 270 120.037254 619.69336 50
AGAGG 265 117.81434 3755.5833 21
GGGTA 195 108.35451 4325.9946 24
AGGGT 190 105.57621 4324.09 23
GGTGT 150 104.17507 936.5556 15
TGTGG 145 100.70257 710.25085 35
GGACG 75 97.34369 875.0693 2
GTGGC 60 97.33253 1110.2927 39
GCCGG 25 94.72856 2554.5576 1
AGCGG 70 90.854126 875.0573 7
TGGTG 130 90.28506 936.35846 14
GGCAG 65 84.36455 2269.9602 72
GCGGT 50 81.110435 815.5294 69
GTGAG 145 80.571304 594.2931 54
TTGGG 115 79.86755 936.47986 2
GCGGA 60 77.87496 875.25854 4
CCGGG 20 75.78285 1297.4989 41
GCCGC 1065 73.97515 259.35687 30
GGGTT 105 72.92255 470.56793 26
CGGAG 55 71.38538 1312.888 4
GACGG 55 71.38538 875.03894 8
GGAGC 55 71.385376 1335.2402 42
GCGTC 2385 70.923546 1453.5146 6
TGGGT 100 69.45004 473.50055 35
AGGCG 50 64.895805 1350.3793 70
GGGTC 40 64.88835 1094.1593 16
GGGCT 40 64.88835 1093.6578 1
GCCGT 2030 60.36679 1427.65 42
GCCCG 865 60.083107 157.30685 58
GTTGG 85 59.03254 722.17914 47
GGGCA 45 58.40622 1312.604 2
GTCGT 4465 56.844616 617.9895 8
GGGCC 15 56.837135 1282.0215 23
CGGGT 35 56.777306 1093.5597 10
GGTGC 35 56.777306 1093.6578 1
TGGGA 100 55.566418 936.5775 3
GTGGA 100 55.566418 561.95245 2
GGTTG 80 55.560036 475.6293 41
GATGG 95 52.788097 770.4129 47
GCGAA 6315 51.466064 101.65044 7
GCCGA 2140 50.91617 89.619125 41
GTCGC 1710 50.850845 1373.8414 39
AGTGG 90 50.009777 323.93863 72
ATGGG 90 50.009777 561.95245 2
GGTGA 90 50.009773 561.9524 2
GCTCG 1680 49.958725 112.993 46
GGCTG 30 48.666264 573.56616 53
GATCG 4740 48.282093 207.28215 68
GACCG 1985 47.228313 113.303246 33
GGAGA 105 46.681152 602.4654 26
GAAGG 105 46.681152 450.08844 18
GCGCC 665 46.19106 213.89395 40
GACGC 1930 45.91972 97.70661 39
GCAGG 35 45.427063 875.7139 17
GGATG 75 41.674812 374.61676 1
CAGGG 30 38.937485 441.75153 34
GAGGC 30 38.937485 652.49854 69
AGGGC 30 38.937485 875.1403 15
GGGAC 30 38.937477 438.9022 22
AGGTG 70 38.896492 380.54715 41
GCGAC 1570 37.354385 90.80335 63
GCGAT 3650 37.17925 74.81032 51
GAACG 4530 36.918648 63.516384 27
GACGA 4515 36.796402 81.92128 47
GAGGA 80 35.566593 303.99014 38
AGGAG 80 35.566593 304.28674 39
GCGCA 1445 34.38031 73.32474 41
TGGAG 60 33.33985 374.71594 4
GCGCT 1115 33.15713 93.67982 60
CGAGG 25 32.447903 449.8811 47
ACGGG 25 32.447903 580.5854 65
GTCGA 3170 32.28992 72.4681 26
GTACG 3140 31.984339 73.88969 46
AAGGG 70 31.120768 450.2439 19
GAGGT 55 30.561535 577.80975 47
TGAGG 55 30.561531 378.95068 36
GCGTT 2360 30.04553 75.69222 51
CTCGG 1010 30.03471 1465.7913 32
GCGTA 2870 29.234093 51.527946 16
GACGT 2645 26.942223 72.19951 60
GCACG 1055 25.101194 60.79161 56
GTTCG 1865 23.743607 55.51563 68
TCGTG 1580 20.115227 613.5593 9
GGAGT 35 19.448244 279.34784 69
GGGAT 35 19.448244 190.76688 43
CGGGC 5 18.945711 1281.3331 22
CGTGT 1480 18.842112 605.05206 10
AGCGT 1795 18.28404 491.1464 5
ATCGG 1610 16.399612 102.41667 69
CTGGG 10 16.222088 555.14636 39
TGCGG 10 16.222088 549.1013 24
CCCCG 11565 14.725743 26.822317 31
AGAGC 1785 14.547415 412.09766 3
TCGGT 1100 14.004272 610.6036 33
TCGGA 1350 13.751228 90.081535 70
GTGTA 2900 12.646535 205.78864 11
CCGCC 9355 11.911745 17.757746 33
CCCGC 9215 11.733482 18.612387 32
TGGTC 870 11.076106 560.4419 37
CGGAA 1330 10.83925 60.574116 71
TGTAG 2460 10.72775 204.31012 12
CGCCC 8380 10.670275 17.782526 58
CCGTC 18365 10.0112505 13.849041 43
AAGAG 3340 9.323946 143.98279 2
CCCGA 19980 8.714304 11.943056 47
CCCCC 368010 8.5898695 10.513187 15
CGTGC 280 8.326454 35.98478 61
GCTGG 5 8.111044 549.9522 28
CGTCC 14580 7.947946 12.727313 50
CCTCG 14145 7.710816 10.631436 40
AGTGT 1740 7.5879207 182.95724 23
CTCGC 13835 7.5418267 11.093065 68
CCGAC 17235 7.5170693 10.498176 28
TCGCC 13185 7.1874943 30.96173 40
CGACC 16425 7.163787 9.707707 41
CCGTG 240 7.1369615 38.852287 64
CTCCG 12700 6.923108 10.471887 9
TCCCG 12535 6.8331623 9.603386 46
TCCGT 29040 6.777348 8.746732 53
CTGCG 225 6.6909003 40.69882 40
TACCG 35825 6.689432 7.868182 3
ACCGA 44335 6.6235323 8.012741 72
TCCGC 12110 6.6014833 10.104335 51
ACCCG 15085 6.5793443 9.491496 46
CCGAT 35060 6.5465875 9.142497 48
TGCGC 220 6.542214 40.73129 41
GAAGA 2330 6.5044293 143.9758 1
TGTGT 1190 6.486056 36.08709 50
CCGAA 42475 6.345653 7.729864 48
CCGCT 11565 6.3043895 9.108226 36
CGCTC 11535 6.2880354 9.497995 50
TCTCG 26590 6.2055683 17.206638 31
TTCCG 25900 6.0445356 8.129437 67
TGCCG 200 5.9474673 57.265694 59
CCCGT 10675 5.8192263 9.069634 47
CGCCT 10630 5.794696 9.041473 24
ACCGC 13190 5.7528367 10.244787 46
AGAGT 1645 5.7395697 147.36983 21
CAGCG 240 5.7102246 48.883156 41
CGAAA 111470 5.704356 7.3803096 49
CTCGA 30435 5.682983 7.5797963 71
CTGTG 445 5.6653647 39.235252 41
GTGTC 435 5.538053 44.524834 51
GTAGA 1580 5.512778 172.57826 26
TCCGA 29260 5.463581 7.313625 47
ACGCC 12435 5.4235425 7.9020295 58
TGTCG 425 5.410742 44.12989 59
GGAAA 1905 5.3179984 132.78775 17
CACCG 12170 5.307963 8.632862 48
TCGTC 22680 5.293053 7.3804426 49
AGCGC 220 5.234372 29.52865 62
CCGTA 27895 5.2087016 14.616 43
TTGTG 955 5.205196 49.247578 49
ATCCG 27740 5.1797585 6.6111727 56
CGAGA 635 5.175131 32.616833 71
TTCGC 21915 5.114517 7.192366 41
CGTTG 400 5.0924625 39.579212 46
CGAAC 33950 5.0720406 7.2712736 34
CGCTG 170 5.055348 68.718834 59
CGCCA 11585 5.052814 7.5785327 49
TTTCG 50360 5.0316997 7.385834 46
GAAAG 1800 5.024881 136.5984 18
CTTCG 21430 5.0013285 6.8419456 35
TCGCT 21410 4.9966607 6.959057 58
CGTGA 490 4.9911866 42.5945 50
AACCG 33085 4.942812 6.9551287 45
CGTCT 21170 4.9406495 6.672275 34
TCGAC 26360 4.9220777 7.1136656 33
GTGTT 895 4.878168 30.499176 51
CGAGC 205 4.8774834 24.872976 50
TGCGT 380 4.8378396 44.364174 50
CGATA 75630 4.837298 6.7348695 49
CGGAC 200 4.75852 32.089954 5
TCGGC 160 4.7579737 46.409023 70
ACGTC 25460 4.754025 5.8270097 29
CGTAC 25185 4.7026753 7.202842 49
ACCGT 25035 4.674667 6.1919465 71
CGATC 25020 4.671866 6.683718 49
CCGTT 19990 4.6652617 6.5478206 40
AACGA 91060 4.659896 6.2373247 48
CGTCA 24915 4.65226 6.0997043 49
ACGAA 90400 4.626122 6.539418 48
CAGTG 450 4.583743 35.39856 49
CGCTT 19605 4.5754104 6.430143 33
CTCGT 19560 4.564908 6.5523844 47
TTGCG 355 4.519561 25.750462 2
CCCGG 65 4.514915 47.406643 37
CCACG 10305 4.4945397 6.762334 12
ATCGC 24020 4.4851403 5.8550253 64
ACGAG 550 4.4823966 31.15431 49
CCGCA 10245 4.468371 7.2565413 47
GCTGC 150 4.4606004 51.537483 47
TGCGA 435 4.4309516 21.29725 50
ACGAC 29485 4.4049816 6.593629 49
CCGAG 185 4.4016314 27.206213 71
TGTGC 345 4.392249 44.364174 50
CTACG 23505 4.3889775 6.1128607 59
CGTTT 43900 4.3862514 5.8333244 49
TCGAA 68470 4.379344 5.521815 71
AAACG 85265 4.3633437 5.7470474 47
GTGAT 995 4.33907 35.078087 51
GTTTG 795 4.3331213 28.241108 46
CGCTA 23040 4.3021502 5.5035405 26
CGACT 23000 4.2946806 6.609489 34
ACTCG 22915 4.278809 6.321002 46
AACGC 28300 4.2279453 5.979503 57
TCGAG 415 4.2272296 31.858704 49
ACGTG 415 4.2272296 31.858704 49
TTCGT 42165 4.2128997 5.9567394 47
GTGCT 330 4.2012815 40.07235 51
TACGC 22345 4.1723757 5.553919 38
TATCG 51940 4.1521287 5.252246 56
CACGC 9515 4.1499805 5.9104033 26
TGTGA 950 4.1428304 30.392614 50
GTGAC 405 4.125369 39.18636 51
TAACG 64260 4.1100726 5.0894275 56
GTTGT 745 4.060598 49.119984 47
ATCGA 62960 4.026924 5.174731 32
TGTTG 735 4.0060935 45.185772 46
CGACA 26815 4.0060906 5.3622212 50
TACGA 62215 3.9792738 5.298559 47
CGAGT 390 3.972577 31.945875 50
ACGAT 62050 3.9687204 5.397065 48
CGCAA 26495 3.9582832 5.440743 34
CGTAA 61695 3.9460146 5.690187 49
AGCCG 165 3.9257793 41.093082 46
ATGTG 900 3.9247866 32.852036 41
GTGCA 385 3.9216466 42.748753 51
CGAAT 61295 3.9204307 5.223414 49
GCCCC 3075 3.915405 7.846465 59
CAACG 26090 3.8977773 5.9033556 47
TTCGA 48755 3.8975172 5.3201175 47
TCGTT 39005 3.8971694 5.355222 58
TCGAT 48710 3.89392 5.329822 48
CACGA 26040 3.8903074 5.7388315 32
GTCTG 305 3.8830028 18.353518 55
CGTTC 16585 3.8706033 6.0181007 50
CGGTC 130 3.8658535 22.906277 59
TTACG 48105 3.8455555 5.0257053 46
ACGCT 20505 3.8288014 5.145008 58
CGATT 47815 3.8223727 5.195033 49
CGGCC 55 3.820313 46.828587 1
GGCCC 55 3.820313 46.82908 6
AACGT 59645 3.8148966 4.9660134 47
CCGGA 160 3.8068163 24.060684 1
TCGTA 47340 3.784401 5.9682903 48
CGCAC 8670 3.7814326 5.7113485 59
TAGCG 370 3.7688553 24.778992 49
CATCG 20070 3.7475758 5.24251 47
AATCG 58115 3.7170377 4.693918 31
ACACG 24845 3.7117777 5.025175 31
CGTAT 46365 3.7064586 7.512423 44
GTTGC 290 3.6920354 52.95418 47
GAGCC 155 3.6878536 32.81938 45
AGCGA 450 3.6674154 17.039757 50
GAGTC 360 3.6669946 17.811981 51
TGACG 360 3.6669943 31.667007 46
ATCGT 45195 3.6129277 4.9321923 47
TACGT 45105 3.605733 5.153864 47
ACGTA 56190 3.5939147 5.330435 48
GTGAA 1025 3.5763278 28.065691 51
AGTGA 1025 3.5763276 29.180262 50
ATACG 55890 3.5747273 4.882675 46
GGTTT 655 3.570056 22.047823 8
CGCAG 150 3.5688903 82.186165 46
TGAGC 350 3.5651333 28.396334 50
AAGCG 435 3.545168 16.993258 49
GAGTT 810 3.5323083 35.078087 51
TCGCA 18915 3.531908 4.9335694 58
CGTAG 345 3.5142028 52.778347 46
TGAGT 805 3.5105035 25.833723 50
CGAAG 430 3.504419 28.249619 59
GCTGT 275 3.501068 30.889938 47
TAGTG 800 3.4886992 31.825165 49
GAGCT 340 3.4632728 35.623962 51
ATGCG 340 3.4632723 31.858704 49
GTGCC 115 3.4197936 20.04924 2
CGTTA 42725 3.4154737 5.0561285 49
CGATG 335 3.4123418 49.25979 46
ACGTT 42315 3.382698 4.802392 48
CGGCA 140 3.3309643 40.80932 71
GACTG 325 3.310481 28.14845 46
TGCTG 260 3.3101008 34.32325 59
CACGT 17495 3.2667582 4.992711 48
GTTGA 745 3.248851 27.208036 47
ACGCA 21665 3.2366939 4.4615326 48
TCACG 17315 3.233148 4.515002 46
ACCGG 135 3.2120013 24.740835 47
CGGTT 250 3.182789 13.940964 56
AGACG 390 3.1784267 42.227528 46
ATTCG 39690 3.1728532 3.9386835 40
TTGAG 725 3.1616337 27.278713 49
CCGGC 45 3.1257107 24.07617 47
GCCTG 105 3.1224208 30.9789 48
GAGAC 380 3.096929 19.825472 49
CAGAG 380 3.0969286 22.65768 49
CGCAT 16585 3.0968382 4.7370043 49
GCTGA 300 3.0558286 28.245476 47
AGTCG 300 3.0558286 31.667007 46
GAGCA 370 3.015431 22.801956 51
GTAGT 685 2.9871988 46.858284 47
GACAG 365 2.9746814 25.336515 46
GAGAT 850 2.9657352 17.083464 51
ATGAG 845 2.9482894 20.612951 49
AAGTG 845 2.9482892 30.313162 49
GATGA 840 2.930844 33.862778 47
GATTG 670 2.9217856 21.089117 46
TGATG 665 2.8999813 24.101847 46
CCGGT 95 2.8250468 30.072718 6
GATGT 640 2.7909594 40.812054 47
GCAGA 340 2.770936 31.07359 47
GCAGC 115 2.736149 57.728615 47
TTTGG 500 2.7252336 14.698905 3
CGGTA 265 2.6993153 17.168112 1
AGTGC 265 2.6993153 35.49542 50
TGGCC 90 2.6763601 20.365644 41
AGATG 765 2.6691616 43.38831 46
AGTTG 610 2.6601331 40.671867 46
TGAGA 760 2.651716 21.885197 50
AGCTG 260 2.6483848 49.25979 46
GATGC 255 2.5974543 28.245476 47
GTATG 590 2.5729158 28.620943 46
GAAGT 725 2.5295975 36.281548 47
AGAGA 895 2.4984825 17.510168 50
GTAGC 245 2.4955933 52.14409 60
CTGAG 245 2.4955933 24.778992 49
GTCAG 245 2.4955933 21.11134 46
TGAAG 715 2.4947064 40.977844 46
GCTTG 195 2.4825757 17.378044 37
TAGAG 710 2.477261 27.888111 49
GAGAA 880 2.4566085 23.43144 51
ACGGA 300 2.4449437 13.736589 3
GAAGC 295 2.4041946 28.248718 47
GCCAG 100 2.37926 32.874466 46
TCCGG 80 2.3789868 30.072718 6
TGGTT 430 2.343701 14.698858 7
GCTAG 230 2.3428018 17.592783 46
GAGTA 670 2.337697 21.964453 51
TTCGG 180 2.291608 12.976637 31
ACGGC 95 2.260297 16.186178 33
GATAG 645 2.2504694 26.515078 46
AGCAG 275 2.2411983 22.521347 46
GCAGT 220 2.240941 21.184107 47
GGCTC 75 2.2303002 20.395 42
TGCAG 215 2.1900105 21.11134 46
TTGGT 400 2.1801867 9.463381 48
AACGG 265 2.1597 10.989387 2
GACCC 4935 2.1524072 5.0155177 50
AGTAG 615 2.1457965 25.309847 46
GCATG 205 2.0881495 17.592783 46
CTGGC 70 2.0816135 20.173763 29
AGAAG 730 2.0378685 34.714634 46
GGCTT 160 2.0369852 17.16614 1
GGTTC 160 2.0369852 12.878233 5
TACGG 195 1.9862885 17.168945 2
TTGGC 155 1.9733293 13.067991 40
TGGCA 190 1.935358 10.300867 1
CGGAT 190 1.935358 10.301368 2
ACGGT 180 1.8334972 10.302201 15
GTTAG 420 1.831567 19.58275 46
GGACC 75 1.7844449 16.044975 5
CGGCT 60 1.7842401 20.048965 7
GGACT 170 1.7316362 14.074225 46
GGTCA 170 1.7316362 10.648625 50
ATGGT 390 1.7007409 18.17178 48
GGCAT 165 1.6807057 14.198167 50
GGCAC 70 1.6654822 8.290992 50
ATGGC 155 1.5788448 10.301368 2
GTAAG 450 1.570095 15.668 46
TGGTA 355 1.5481102 15.15484 49
AGGAA 550 1.5353804 12.344373 33
GAATG 440 1.535204 10.884465 47
GGATT 350 1.5263059 7.435049 36
CCTGG 50 1.4868668 30.072815 8
ATTGG 340 1.4826971 7.626849 72
GGCAA 180 1.4669662 13.736734 2
GGACA 180 1.466966 11.359837 50
TGGAA 420 1.465422 8.2329645 1
TCTGG 115 1.4640833 13.238547 47
TTGGA 335 1.4608928 8.820448 2
AAGGA 520 1.4516321 11.387714 32
GGCCA 60 1.4275562 16.044704 4
CACGG 60 1.4275562 9.163564 59
GGAAC 175 1.426217 11.320098 48
CTGGT 110 1.4004272 8.841797 48
GCAAG 170 1.385468 14.075842 46
TGGAC 135 1.375123 10.619569 49
AGGTT 315 1.3736755 7.350273 7
GGCCT 45 1.3381801 20.395 42
GGTCT 105 1.3367715 8.872834 50
TATGG 305 1.3300666 7.557788 47
CATGG 130 1.3241924 14.122738 47
CAGGC 55 1.308593 16.523977 48
AAAGG 465 1.2980943 8.5362835 31
GGTAA 370 1.290967 9.726754 50
GGAAT 370 1.2909669 10.942597 50
ACTGG 125 1.2732619 8.217379 61
GGTAT 290 1.2646534 8.9054575 34
GGTAC 120 1.2223314 17.313297 32
TGGCT 95 1.2094599 8.848623 49
AATGG 345 1.2037395 6.046925 47
GGTCC 40 1.1894934 20.725098 50
AGGCT 115 1.171401 7.0797124 49
TCAGG 115 1.171401 14.122738 47
GGATC 115 1.1714009 10.648625 50
ATAGG 335 1.1688485 7.1641626 39
GGTTA 265 1.1556317 5.880299 2
CAAGG 140 1.1409738 8.474616 47
CAGGA 140 1.1409737 11.081783 32
AAGGT 325 1.1339574 6.057955 48
TAAGG 315 1.0990666 8.284501 29
AGGAT 315 1.0990666 8.307758 33
TTAGG 250 1.0902185 7.479816 71
TAGGA 310 1.0816209 6.0579553 48
AGGCC 45 1.0706671 16.053053 17
TGGAT 225 0.98119676 5.8802376 3
AAGGC 120 0.97797734 8.243822 4
AGGTC 95 0.96767914 14.159425 49
CTGGA 95 0.967679 7.0742497 48
TAGGT 220 0.9593923 7.412174 32
GGATA 270 0.942057 7.1251845 34
ACAGG 115 0.9372285 11.075942 31
AGATC 13780 0.8813695 46.832653 72
CCAGG 35 0.83274096 11.961202 69
AGGAC 100 0.81498134 8.37211 41
CAGGT 75 0.76395714 10.300976 6
GGCTA 70 0.71302664 10.400649 34
CTTGG 55 0.70021373 8.825699 47
CTAGG 65 0.66209626 10.592053 47
TAGGC 65 0.6620962 6.925319 32
AGGCA 80 0.65198505 5.6644206 49
CAGAT 6280 0.40166906 7.7930126 72
TAGAT 14025 0.38404083 8.316922 72
AAGAT 16685 0.3655448 8.723393 72
Produced by FastQC (version 0.10.1)