FastQCFastQC Report
Wed 2 Apr 2014
mcf_T3D5_R1.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename mcf_T3D5_R1.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 7125800
Filtered Sequences 0
Sequence length 19-76
%GC 16

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CCCCC 440 3032120.8 1.9523448E7 16
CCCCA 160 9157.692 182858.39 23
ACCCC 140 8012.9805 182750.2 22
CACCC 135 7726.8027 101326.445 13
CCACC 130 7440.625 61128.395 30
CGCCC 50 6520.064 92341.92 15
CCCAC 90 5151.2017 61646.13 44
GCCCC 30 3912.0383 49884.887 70
CTCCC 75 3491.9922 49601.016 24
CCTCC 65 3026.393 33055.848 23
TCCCC 60 2793.5938 34295.676 63
CCGCC 20 2608.026 48889.83 67
CGCGC 980 2418.2214 6989.51 12
CCCTC 50 2327.9946 17811.443 70
CCCGC 15 1956.0193 46175.566 18
CCCCG 15 1956.0193 48181.066 64
CCCCT 40 1862.3958 17203.092 64
CACAC 1330 632.25165 9252.371 7
CCGTC 625 550.65564 16143.582 45
CACCA 775 368.41733 6926.286 26
CGCTC 360 317.17764 16836.031 2
CGCCG 125 308.4466 2745.5505 65
CGCGG 6600 308.17828 480.687 23
CGGCG 6325 295.33752 488.0393 49
ACTCC 735 284.23114 6846.7524 18
TCGCG 16860 281.09024 392.97556 63
CTCCA 710 274.5634 6712.9277 19
GCGCG 5405 252.37933 381.00897 22
CGCGA 12110 248.19124 355.50104 52
TCACC 610 235.8925 5495.066 25
CGTCG 13560 226.07257 324.47363 10
CGACG 10265 210.37845 295.71664 50
CTTCC 620 195.03918 6122.727 6
CACGC 180 194.95155 1175.2722 53
CGCGT 11110 185.22612 270.6289 50
ACGCC 160 173.29028 1540.2583 27
ACGCG 8430 172.77061 259.75186 54
GCCGC 65 160.39224 1746.4088 1
CGGAC 7560 154.94019 269.69138 60
ACACC 325 154.49759 1353.2843 29
ACCAC 320 152.1207 1375.5974 53
CCGGC 55 135.7165 943.9677 70
GCGCC 55 135.7165 1823.4526 64
GCACC 115 124.552376 1150.5507 18
CGCAC 115 124.55237 783.5148 53
CCGCG 50 123.378654 1747.3774 13
CCGAC 110 119.137054 782.78326 52
GCCAC 105 113.721725 803.38245 65
CCACG 100 108.30641 795.46954 62
CGCCA 100 108.306404 1155.6147 28
CAACC 215 102.20609 842.3748 20
CTCGC 115 101.32064 637.37225 53
CGGCC 40 98.70291 908.798 63
CGACC 90 97.47578 1173.0653 51
CCCAG 90 97.47578 1154.6589 26
CACTC 250 96.677246 1119.0186 53
CCACA 200 95.07544 860.7096 54
CCAAC 190 90.32166 842.0541 19
TCGCC 100 88.104904 968.33923 61
CTGCC 100 88.104904 1268.2839 47
CCGCA 80 86.645134 776.05493 41
AACCC 180 85.567894 1516.8344 21
GCCTC 90 79.29441 328.2767 66
CCCAA 165 78.43723 1181.6632 24
CACCG 70 75.81449 766.9568 14
CTCTC 240 75.49904 1024.0844 53
TCCGC 85 74.88917 625.51306 23
ACCTC 190 73.4747 699.38654 53
GCGGC 1530 71.44133 185.61288 52
CGCCT 75 66.078674 970.6457 62
CGTCC 75 66.078674 968.3392 61
CCACT 170 65.74053 690.1292 35
ACCCG 60 64.98385 1157.9767 32
CCTCT 205 64.48876 569.5718 54
CTCAC 165 63.80698 558.9868 52
GGCCC 25 61.68932 937.34436 69
TCCCG 70 61.673435 640.59906 57
CCCGT 70 61.673435 641.5875 58
TTCGC 9610 57.20601 83.895805 52
ACCCA 120 57.04526 541.73737 69
CACCT 145 56.072807 278.33682 47
CGGGC 1180 55.09854 119.2159 59
CCAGC 50 54.153206 1149.8136 7
CCCGA 50 54.153206 772.29065 33
GCCCA 50 54.153202 767.1086 16
GCGTC 3180 53.017017 100.35149 12
CTACC 135 52.20572 837.688 51
CCTCA 135 52.20571 684.6752 18
TCGGC 3130 52.183422 94.39744 7
CCCGG 20 49.351463 901.97046 60
GACCC 45 48.73789 1154.6588 26
GTCCC 55 48.4577 625.73065 24
CCTAC 125 48.338623 568.0462 62
CGGTC 2850 47.51525 89.37397 37
CGAGC 2240 45.908207 96.190544 51
TTCCC 145 45.614002 334.14743 13
GGCGC 950 44.358994 100.89621 48
CCTCG 50 44.052452 322.04932 60
GTCGC 2565 42.76373 89.84814 45
ACCCT 110 42.53799 274.97525 27
TCCCA 110 42.537987 416.23184 43
ACGAC 4615 41.514343 74.568085 49
CGTTC 6935 41.28238 65.33813 12
TCGTC 6885 40.98474 67.445854 15
ACGGC 1985 40.68205 88.57049 48
CCCAT 105 40.604446 555.34485 44
CCCTA 105 40.604443 410.76416 15
TGCGC 2400 40.012848 94.57264 21
CTCCG 45 39.64721 328.27673 66
CCGCT 45 39.64721 623.5568 1
TCTCC 125 39.32242 342.12314 55
ATCGC 5350 39.14949 63.248333 48
CATCC 100 38.670902 414.84503 38
CCATC 100 38.6709 411.96738 24
CGTAC 5260 38.490902 58.022137 49
CAGCC 35 37.90725 389.43463 46
TCCTC 120 37.74952 224.97824 38
CGATC 5080 37.17372 201.97401 10
GCCCG 15 37.01359 873.52625 17
TCCAC 95 36.73735 412.46286 27
ACGTC 4850 35.49066 155.36464 10
GCTCC 40 35.24196 337.04446 70
TCCCT 110 34.603725 334.14743 14
TCGAC 4640 33.95395 59.55677 3
CCCTT 105 33.03083 335.65192 28
CTCCT 105 33.03083 228.39174 56
AGCCC 30 32.491924 398.8885 63
GCGAC 1585 32.48415 88.87525 52
CCTTC 100 31.457933 230.49757 61
AACGC 3490 31.394379 61.822002 53
TACGC 4240 31.026886 49.1988 4
ATCCC 80 30.936714 553.50256 39
TGCCC 35 30.836718 337.04453 70
GCCCT 35 30.836716 324.4871 63
AGCGC 1460 29.922312 65.548546 26
CGTGC 1760 29.342752 88.49759 9
GACGC 1305 26.745628 72.75261 23
CCCTG 30 26.431473 325.53268 64
CCTGC 30 26.431473 317.29672 48
TACCC 65 25.13608 145.73508 68
CGAAC 2755 24.782665 47.898727 23
ACCGC 15 16.245962 391.39163 52
CGGGG 18205 16.085587 25.013943 1
GGGCG 13915 12.295026 17.509058 4
ACACG 1355 12.188934 175.08186 8
GGCGG 13715 12.11831 17.510004 6
GCGGG 13235 11.694191 15.964232 19
GCACA 1255 11.289382 162.34863 6
GACGG 28970 11.23515 14.41436 37
GCCGT 665 11.086892 311.25046 44
TGCCG 665 11.086892 317.02527 43
AGCAC 1145 10.299873 159.16531 5
CACGT 1360 9.952021 132.06708 9
CGTCT 1650 9.822051 113.44215 46
TCGGG 30305 9.560721 12.724213 35
GGACG 22770 8.830666 12.248131 56
CGAGG 21215 8.227605 11.033747 48
GGTCG 25360 8.000654 11.05242 1
GTCGG 25330 7.991192 11.400087 19
GCGAG 20595 7.9871564 11.665445 6
GGCGA 20095 7.7932463 10.583227 20
GTCAC 1060 7.7567215 119.53291 24
ACGGA 44680 7.6054993 9.333223 50
CGGAG 19485 7.556676 10.161289 14
CGGGA 19065 7.3937917 9.834278 36
CGGTA 53230 7.370839 8.940895 34
TCGGT 65245 7.3494296 8.56443 65
ATCGG 52840 7.316835 10.437248 2
TTCGG 64595 7.276211 8.480072 24
CGGGT 22670 7.1520047 10.382576 1
GCGGA 18155 7.040875 9.749352 15
AGCGG 17910 6.945859 9.700867 55
GGGGG 413525 6.9141073 8.070393 1
TTTCG 170915 6.874113 7.7096834 23
GAGCG 17380 6.7403145 9.640195 24
ACGGG 17205 6.672446 9.198376 17
GCGGT 20610 6.5021095 9.18325 23
CGGAA 37880 6.447992 8.49302 4
AGGCG 16160 6.267174 9.36476 24
CGAGA 36540 6.219896 8.131941 1
TCGGA 44755 6.1972933 9.309902 3
CAGTC 840 6.1468363 132.40117 22
TCGAG 44280 6.131519 7.381251 26
GGCGT 19330 6.098291 8.819256 4
CCGAT 820 6.000483 147.60439 9
GTTCG 52920 5.9610972 8.052111 7
CGGAT 42885 5.938351 7.3709946 54
ACCAG 650 5.8470902 137.52267 27
TACGG 41860 5.7964177 6.8108892 11
CGGTT 51000 5.7448215 6.9063644 20
TCCGA 780 5.7077765 145.01483 8
AGACG 33525 5.7066774 7.072253 24
TCCAG 775 5.6711884 137.45016 20
CCAGA 630 5.66718 143.97154 28
GACGA 33225 5.655611 6.581967 52
TCGTT 140375 5.645809 6.561292 5
CCAGT 770 5.6346 132.31218 21
TTCGT 138415 5.5669794 6.219371 4
TGGCG 17645 5.566703 8.159601 64
CGAAA 74225 5.545604 6.587638 27
GCGAA 32355 5.507518 6.445752 58
GTCGA 39425 5.45924 6.713254 5
CGTTT 134800 5.4215856 6.504269 1
TATCG 108740 5.376255 6.4734855 2
CGGTG 16800 5.3001184 6.69861 47
AGCGA 30915 5.262399 6.30282 56
GACGT 37910 5.249456 6.3212395 5
AACGG 30770 5.237717 6.930887 57
ACGGT 37705 5.221069 6.3456893 25
GTCGT 46175 5.201317 6.4025803 25
TTCCG 870 5.1789 117.9664 7
GATCG 37390 5.17745 8.771209 1
GTACG 37250 5.1580644 6.788412 25
TCTCG 860 5.1193724 110.55424 36
TTCGA 103455 5.1149573 5.645923 52
CGAAG 30000 5.1066465 6.987446 1
CTCGT 850 5.0598445 114.87979 37
CGTGG 16030 5.057196 7.08767 63
GCGAT 36200 5.012669 6.1742325 7
ATGCC 680 4.97601 141.67268 42
AAACG 66525 4.970311 5.794494 16
AAGCG 29135 4.959405 6.360299 45
GCGTT 43935 4.9489946 6.577219 9
TAGCG 35115 4.862427 6.223235 5
TGACG 35050 4.853427 6.2335577 48
TCTGC 815 4.851498 113.9045 51
TGCGG 15225 4.803233 7.814575 3
CTGCT 795 4.732443 109.7099 52
AGTCG 34135 4.726725 5.83122 7
GCACG 230 4.713789 29.685925 54
CGTTA 94680 4.681109 5.6686735 1
ACGAG 27415 4.666624 6.0234194 11
TTACG 94275 4.661086 5.346231 21
CGTAG 33575 4.6491814 6.272237 6
GCGTG 14680 4.631294 6.363604 5
GCGTA 33260 4.6055627 5.9292235 5
CGAGT 32880 4.5529437 5.7476606 58
TTGCG 40340 4.544041 6.178513 2
ACGAA 60755 4.539215 5.3227963 62
ATTCG 91770 4.537235 5.108876 6
TCGAT 91670 4.532291 5.212983 31
TGTCG 40205 4.5288343 5.779668 4
CGATA 74455 4.5252075 5.4282746 48
GGCAC 220 4.508841 22.71737 64
ATCGT 90310 4.46505 5.293421 45
ACGTT 89795 4.4395885 5.1435914 4
CGTTG 39300 4.426892 5.620533 6
TCGTG 39155 4.4105587 5.2219133 6
TCGTA 89175 4.4089346 5.4452977 38
TCGAA 71910 4.3705277 5.3981743 11
AGCGT 31335 4.3390055 5.782219 5
CGATT 87305 4.316479 5.058874 44
GGGGC 4865 4.29862 7.8169665 7
AACGA 57445 4.2919135 5.357334 42
ATCGA 69935 4.250492 4.9067206 49
GAACG 24885 4.2359633 5.5428286 39
GTGCG 13425 4.235363 6.1713014 28
CGTAA 69645 4.2328663 5.355375 1
AATCG 69540 4.226485 5.093473 24
CGTGT 37445 4.217938 5.939345 1
GCTCG 250 4.1680045 17.944824 43
TACGA 68290 4.1505127 4.921925 67
TACGT 82990 4.1031394 5.0386195 4
CAGCG 200 4.098947 29.571669 50
ACGAT 66465 4.039593 4.6228557 43
ACACA 1015 4.007529 25.73543 54
CACGG 195 3.9964733 22.126867 47
ATACG 65425 3.9763844 4.6650023 66
GCCGG 85 3.9689627 17.73729 69
CGATG 28560 3.9547465 5.246036 15
CGTAT 79650 3.9380054 5.3605413 39
ACGTA 64665 3.9301934 4.560175 50
ACGCT 535 3.91495 139.83379 1
CGCAG 190 3.893999 21.859634 27
AACAC 985 3.88908 16.986813 42
CACGA 430 3.8680751 16.063873 36
TAACG 63370 3.8514862 4.462762 53
TGCGA 27780 3.846739 5.096193 6
GCTCT 645 3.839529 115.85423 3
AACGT 62960 3.8265674 4.410669 17
GCGCA 185 3.7915258 22.059383 43
AACTC 1165 3.7418165 60.26128 17
TGCGT 33155 3.7346973 5.221632 4
ACGTG 25870 3.5822585 4.75318 6
CGAAT 58475 3.553979 4.2182374 18
CACAA 900 3.5534737 21.446192 54
GGCCG 75 3.5020258 33.06548 14
ACCGG 170 3.4841049 29.227985 33
CGTGA 25150 3.4825587 4.824651 27
TCTTC 1635 3.4750905 43.62429 5
ATGCG 24895 3.4472485 4.6131396 54
GACAC 380 3.4182987 13.003006 52
CGGCA 165 3.3816311 22.239578 53
TCACG 460 3.3661246 15.787157 46
GCAGC 160 3.2791576 15.052606 62
CACAG 360 3.2383885 16.30525 55
TGCAC 435 3.183183 18.493416 51
CAAAC 780 3.0796776 22.449615 26
CTCGG 180 3.0009632 18.01254 48
TCACA 930 2.9870296 16.249573 52
TACAC 930 2.987029 10.446153 52
GCCGA 145 2.9717364 22.027836 41
CCGGT 175 2.9176033 17.754799 23
CGCTG 175 2.9176033 12.0878525 55
ACACT 885 2.8424957 10.467606 54
CTCTT 1335 2.837459 42.117725 4
CGGCT 170 2.834243 25.002579 67
ACGCA 315 2.8335903 15.983606 26
CCAAA 715 2.8230374 15.428791 25
CACTT 1070 2.795672 10.419002 55
CGACA 310 2.7886124 19.522738 53
CAACA 700 2.7638128 12.699444 37
AAACC 695 2.7440715 12.61858 24
AACCA 690 2.72433 11.190056 19
ACCAA 680 2.6848469 11.194319 20
TCCGG 160 2.667523 13.008324 72
CCGGA 130 2.6643155 21.859634 27
CCGAG 130 2.6643155 22.218815 52
GACCG 130 2.6643155 14.982855 60
CGCAA 295 2.6536794 15.978097 25
ACAAC 660 2.6058812 12.723304 40
ATCAC 805 2.5855467 12.6035 34
GGCTC 155 2.5841627 24.37645 60
CCGGG 55 2.5681522 33.60814 47
AACCG 285 2.5637243 15.978099 25
ATCTC 980 2.560522 48.493317 35
GCAAC 275 2.4737687 9.910306 62
CACAT 770 2.473132 13.972329 55
TCACT 930 2.4298832 11.190766 35
ACCGA 270 2.4287915 16.238398 51
GCGCT 145 2.4174426 17.944826 43
TCGCA 330 2.4148285 13.249231 54
CTTCT 1120 2.38049 41.36574 49
CCAGG 115 2.3568945 14.541913 22
TTCAC 900 2.3514998 9.432985 51
CACTG 320 2.341652 13.263966 55
GAACC 260 2.3388362 19.135288 21
GACCA 255 2.2938585 9.761369 53
TCGCT 385 2.291812 10.6570015 40
CGTCA 310 2.268475 10.524771 46
CCGTG 135 2.2507224 18.999344 69
TCATC 860 2.2469888 41.919064 33
GCACT 305 2.231887 13.059258 35
CGCTT 370 2.2025206 10.678692 43
CGCAT 300 2.1952984 10.501761 43
CATCT 835 2.1816692 45.671 34
TGGCC 130 2.1673625 12.21587 61
CTGCG 130 2.1673625 11.938916 40
GTGCC 130 2.1673625 12.566031 68
GCTGC 130 2.1673625 12.366772 65
AGCTC 295 2.1587102 15.851499 51
AGCCG 105 2.151947 14.950314 59
ACTGC 290 2.122122 10.587548 53
ATCCG 290 2.1221218 7.7987 25
GCCAA 235 2.1139479 9.603032 29
GGGCC 45 2.1012156 33.05933 17
CACTA 650 2.0877085 11.643606 55
GAGCC 100 2.0494735 22.38666 58
CATCG 275 2.0123572 7.949539 54
CTCTG 335 1.994174 10.765924 53
CAGAC 220 1.9790152 12.828711 33
ACCGT 270 1.9757688 7.801388 26
TTTCC 925 1.9660299 9.031501 18
GGCCA 95 1.9469998 15.144915 64
TTGCC 325 1.9346465 10.855912 59
TGCTC 325 1.9346465 10.745708 51
CCTGT 320 1.9048827 10.904216 61
AGACC 210 1.88906 12.756859 21
TACCG 250 1.8294154 10.558588 50
CTGAC 250 1.8294154 13.234435 53
CCGTT 305 1.8155913 6.341571 24
ACTCT 690 1.8028165 6.622909 54
CAACG 200 1.7991048 9.668431 41
CGACT 245 1.7928272 8.1420145 65
CTTCG 300 1.7858275 6.4363294 49
GATCC 240 1.7562388 10.625577 56
TCCGT 295 1.7560637 6.730068 68
CTTGC 295 1.7560637 8.703632 60
CAGGC 85 1.7420524 14.950314 59
CATGC 235 1.7196506 7.887207 45
TGCCA 235 1.7196506 7.7682757 3
ACCAT 535 1.7183449 9.106334 20
TACCA 530 1.7022854 7.959036 17
TCCTG 285 1.6965362 8.452488 23
CTCGA 230 1.6830623 7.9583793 55
GCCTG 100 1.6672019 11.829529 22
TCAAC 515 1.6541077 6.9000864 40
TAACC 515 1.6541077 8.200355 59
CAACT 515 1.6541077 12.65778 41
ACATC 515 1.6541077 8.054951 41
CCGTA 225 1.646474 7.8153653 30
CTGCA 225 1.646474 7.9583793 55
TGACC 225 1.646474 10.577662 52
GGACC 80 1.6395786 21.867605 28
TCTCT 765 1.6259596 7.6879644 53
TTACC 620 1.6199222 7.6237817 59
CAGCA 180 1.6191944 9.644492 37
AGCCA 180 1.6191943 9.864385 60
AAGCC 180 1.6191943 9.574079 22
ACTCG 220 1.6098857 7.826908 33
TCAGC 220 1.6098857 10.541389 48
CGCTA 220 1.6098856 7.7987 25
TTCTC 755 1.6047055 7.6807876 52
GGCCT 95 1.5838417 11.989429 46
CTGTC 265 1.5774809 6.4595547 53
CAAGC 175 1.5742166 9.632163 35
CCGAA 175 1.5742166 9.5946045 27
AACCT 490 1.5738112 7.9590373 17
GTACC 215 1.5732974 12.957317 16
GACTC 215 1.5732973 10.494272 42
GCCAG 75 1.537105 14.555583 24
CCAAT 470 1.509574 7.970983 21
GCTCA 205 1.5001206 10.5776615 52
CTACA 465 1.4935148 5.742578 38
ACTCA 465 1.4935148 9.304539 54
CCATT 570 1.4892832 6.7164516 62
GCTTC 250 1.4881896 6.4026465 42
CCAAG 165 1.4842614 9.590162 26
CTTTC 695 1.4771792 7.5778317 33
CCTTT 695 1.477179 5.2668667 17
CATCA 455 1.4613961 5.7929535 50
ACTAC 455 1.4613961 9.205658 41
TGCCT 245 1.4584258 6.390208 39
AGATC 23785 1.4455988 47.0424 72
TCCAA 450 1.4453367 5.8834586 61
AGGCC 70 1.4346315 14.504435 4
TGTCC 240 1.428662 6.5135474 59
CTCAG 195 1.4269441 10.406669 27
GCCAT 195 1.426944 7.805001 27
CTGGC 85 1.4171216 11.888164 33
GTCCG 85 1.4171215 11.845321 25
ACTTC 540 1.4108999 5.5668693 24
GTCCT 235 1.3988982 6.324297 16
ACAGC 155 1.3943062 9.617719 32
CAGCT 190 1.3903561 13.475342 63
CAATC 430 1.3810996 6.8515234 27
GTTCC 230 1.3691344 8.575191 48
CATAC 425 1.3650403 9.161005 33
CCATG 185 1.3537675 7.8815584 44
CATTC 515 1.3455805 5.5923676 34
ACCTT 515 1.3455805 5.551163 18
TTCCT 630 1.3390256 5.26785 15
CCTGG 80 1.3337615 6.2119536 66
CTCAA 415 1.3329216 10.479245 55
CTCTA 510 1.3325166 9.47182 55
ACCTG 180 1.3171792 7.887207 45
GCCTA 180 1.3171791 8.042661 61
GCTAC 180 1.3171791 8.024462 60
TACTC 500 1.3063889 6.60309 51
TTCCA 500 1.3063889 9.256228 19
TACCT 500 1.3063886 6.474502 17
AATCC 405 1.3008032 5.6914587 20
ATCCA 400 1.2847437 5.714618 29
CCTGA 175 1.2805909 12.96206 19
CTTCA 480 1.2541332 5.6204834 42
CTACG 170 1.2440026 7.7685423 1
GCATC 170 1.2440025 10.906172 66
ACCTA 385 1.2365658 5.8834586 61
CCATA 385 1.2365658 11.387117 21
TCCTT 580 1.2327538 6.0215883 16
TAGCC 165 1.2074142 5.515466 68
CTCAT 460 1.2018777 10.419002 55
CTATC 450 1.1757499 5.5923676 34
GACCT 160 1.170826 7.8118577 29
AGCCT 160 1.170826 5.559447 69
GTCCA 155 1.1342376 10.699283 60
GCCTT 190 1.1310241 9.108889 70
CTACT 430 1.1234944 5.595383 35
TCTCA 425 1.1104305 8.515169 54
ATTCC 420 1.0973666 5.5506063 14
GGTCC 65 1.0836812 12.161753 59
CTAAC 335 1.0759729 5.728864 34
AGTCC 145 1.0610609 7.792914 23
CCTAA 330 1.0599135 5.700761 23
CCTAG 120 0.87811947 5.288831 52
CTAGC 95 0.6951779 13.044847 33
Produced by FastQC (version 0.10.1)