FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | 3291_5903_10007_H94MGADXX_V_CF71_ATCACG_R1.fastq |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 7948472 |
| Filtered Sequences | 0 |
| Sequence length | 101 |
| %GC | 38 |
Per base sequence quality
Per sequence quality scores
![[FAIL]](Icons/error.png)
Per base sequence content
Per base GC content
![[WARN]](Icons/warning.png)
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| GCCCCAACCAAAGTTATCTTATTAACTAACTAAATATAAATTATTAAAGC | 10191 | 0.1282133220070474 | No Hit |
| GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC | 9013 | 0.11339286343337437 | TruSeq Adapter, Index 1 (100% over 50bp) |
| CCCCAACCAAAGTTATCTTATTAACTAACTAAATATAAATTATTAAAGCT | 7985 | 0.1004595600261283 | No Hit |
| CTCCTGTTCACTTTTCTTCCCCAATTCGAGGCTCTACACGCAAATGGCTG | 7979 | 0.10038407381947122 | No Hit |
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| GGGGG | 558540 | 2.8160534 | 6.014786 | 1 |
| CCCCC | 501895 | 2.3003256 | 14.388274 | 1 |
| CCCCA | 593835 | 1.7335559 | 15.432546 | 1 |
| CTGGG | 554220 | 1.7104023 | 6.806586 | 1 |
| CCCCT | 571430 | 1.6654555 | 10.517435 | 1 |
| CCCAG | 541245 | 1.6104528 | 8.246465 | 2 |
| CCTGC | 529065 | 1.5716687 | 6.0462127 | 1 |
| GCCCA | 517365 | 1.5393987 | 6.385927 | 1 |
| CTGCA | 788765 | 1.4924341 | 5.281964 | 1 |
| GCCCC | 315680 | 1.4747064 | 11.900356 | 1 |
| CCCAA | 792160 | 1.4729247 | 6.4404263 | 2 |
| CCTCC | 504635 | 1.4707788 | 6.8122363 | 1 |
| CCTGG | 482825 | 1.4619205 | 5.6766796 | 1 |
| GGCCC | 305645 | 1.4553177 | 8.948555 | 9 |
| TGGCC | 475730 | 1.4404379 | 6.796236 | 8 |
| CCCTG | 483590 | 1.4365784 | 7.4779453 | 1 |
| GCCTG | 457845 | 1.3862848 | 5.072934 | 1 |
| CTCCC | 475150 | 1.3848436 | 9.655629 | 1 |
| CTCCT | 732710 | 1.3579869 | 8.516072 | 1 |
| CCCTC | 463145 | 1.3498545 | 5.2757273 | 1 |
| CCAGT | 708380 | 1.3403366 | 6.568191 | 3 |
| CTCCA | 719375 | 1.3354293 | 6.412736 | 1 |
| CGGGG | 257615 | 1.2743118 | 6.189853 | 1 |
| CCCAC | 435600 | 1.2716275 | 5.196239 | 1 |
| TCCTG | 669230 | 1.2642149 | 6.197345 | 2 |
| CCCGG | 256555 | 1.2215774 | 6.8126984 | 1 |
| CTGGA | 632785 | 1.2203538 | 5.2231474 | 2 |
| CCGGG | 250400 | 1.2152256 | 5.4805074 | 1 |
| CCCCG | 255605 | 1.1940646 | 10.225946 | 1 |
| CCTGT | 619705 | 1.1706592 | 5.8720202 | 3 |
| CGCCC | 243555 | 1.1377728 | 5.118653 | 1 |
| GCCCT | 381125 | 1.1321902 | 5.0796857 | 1 |
| CCTCG | 373305 | 1.1089599 | 6.5663176 | 1 |
| GTCCA | 553570 | 1.0474182 | 5.0813594 | 1 |
| CTCGG | 325705 | 0.9861851 | 7.751502 | 1 |
| GTCCT | 500935 | 0.9462957 | 5.77597 | 1 |
| CTCCG | 299655 | 0.89017123 | 6.819146 | 1 |
| CCCGC | 186025 | 0.86902004 | 6.041055 | 1 |