##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	3291_5903_10008_H94MGADXX_V_CF34_CGATGT_R1.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	9752597	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05928246599342	34.0	31.0	34.0	30.0	34.0
2	32.14616465747534	34.0	31.0	34.0	30.0	34.0
3	32.240116042937075	34.0	31.0	34.0	30.0	34.0
4	35.579144303819795	37.0	37.0	37.0	35.0	37.0
5	35.39305417828707	37.0	37.0	37.0	35.0	37.0
6	35.121348293177704	37.0	36.0	37.0	33.0	37.0
7	35.21909958957599	37.0	35.0	37.0	33.0	37.0
8	35.24097089216339	37.0	35.0	37.0	33.0	37.0
9	36.947772065225294	39.0	38.0	39.0	34.0	39.0
10-14	37.18478714951515	39.4	38.2	39.4	33.8	39.4
15-19	38.31634519502857	41.0	39.0	41.0	34.0	41.0
20-24	38.26633707924155	41.0	39.0	41.0	34.0	41.0
25-29	38.130885527208804	41.0	39.0	41.0	33.8	41.0
30-34	37.954508035141814	40.2	38.6	41.0	33.0	41.0
35-39	37.81961805660585	40.0	38.0	41.0	33.0	41.0
40-44	37.62037771067542	40.0	38.0	41.0	32.8	41.0
45-49	37.31831798237947	40.0	37.8	41.0	32.0	41.0
50-54	36.97569658625287	40.0	37.0	41.0	31.0	41.0
55-59	36.5694598064495	39.8	36.0	41.0	31.0	41.0
60-64	36.209022437818355	39.0	35.0	41.0	30.4	41.0
65-69	35.57705770063092	37.8	35.0	40.6	30.0	41.0
70-74	34.83300245052677	36.6	35.0	39.4	29.4	41.0
75-79	33.95647467028526	35.4	34.2	38.0	29.0	40.0
80-84	33.195784404912864	35.0	34.0	36.6	28.8	38.6
85-89	32.55115838376178	35.0	34.0	35.8	27.2	37.0
90-94	32.118066398109136	35.0	34.0	35.0	26.8	36.0
95-99	31.77816505695867	35.0	34.0	35.0	26.0	36.0
100-101	31.25813980624853	35.0	33.5	35.0	24.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	135758.0
3	37557.0
4	22737.0
5	15659.0
6	13944.0
7	13443.0
8	13936.0
9	14994.0
10	15882.0
11	17092.0
12	17420.0
13	17477.0
14	17347.0
15	17834.0
16	17881.0
17	18181.0
18	18305.0
19	18800.0
20	19254.0
21	20406.0
22	22396.0
23	25975.0
24	31161.0
25	37956.0
26	46659.0
27	58409.0
28	74212.0
29	82148.0
30	98717.0
31	123767.0
32	158583.0
33	213777.0
34	312460.0
35	491176.0
36	956810.0
37	1980854.0
38	3199784.0
39	1347829.0
40	6017.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.4458581642034	21.028053152088365	16.259274747301312	37.26681393640692
2	19.74143443237025	22.701151256191448	31.32518296100552	26.232231350432784
3	18.59675693925181	22.857475140058785	29.720594166978525	28.825173753710875
4	21.69252670791145	26.16796855769073	24.3615332228367	27.777971511561123
5	24.85572105006208	29.445421230315233	25.6353408206458	20.063516898976882
6	21.554959763367155	34.937650831849076	26.054270851389425	17.453118553394344
7	17.292351932786275	25.10755930860985	41.19325155701828	16.406837201585596
8	18.25785272926411	27.444261428199212	34.26425331509853	20.033632527438154
9	18.540399800384915	25.67072166432873	35.5993467590136	20.189531776272755
10-14	19.272707565264273	31.743827730934367	30.04121854675012	18.942246157051244
15-19	20.039014847710217	30.549821265539222	29.93281747185124	19.478346414899317
20-24	19.3139500419545	30.447622753702174	30.45201602390956	19.78641118043376
25-29	19.59276857023698	30.11092115990872	30.205804687860393	20.09050558199391
30-34	19.2727353359696	30.5924109453159	30.294757398995912	19.840096319718583
35-39	19.51791675688351	30.754190088517387	30.058155710424494	19.66973744417461
40-44	19.507952859711853	30.543903918563377	30.36879999220584	19.57934322951893
45-49	19.61878319475567	30.521285529952262	30.50050498248082	19.359426292811246
50-54	19.38724619089319	30.72712479310131	30.44553153286447	19.440097483141024
55-59	19.16853042521916	30.49952270498459	30.98166819612182	19.35027867367443
60-64	19.431796324385505	30.68563118274705	30.60864174402358	19.273930748843863
65-69	19.214454571419044	30.726579766404015	30.68328304301292	19.37568261916402
70-74	19.119196984898938	30.793373140825576	30.90383009180106	19.183599782474424
75-79	19.257809934707108	30.70856880147711	30.332241583988328	19.70137967982745
80-84	19.05112600921764	30.822231334247434	30.862751405547666	19.26389125098726
85-89	19.278959297883027	30.549347643494723	30.80352564738899	19.368167411233262
90-94	19.083266580943047	30.590440051417765	30.772071223751073	19.554222143888115
95-99	19.36613209435784	30.63207845273298	30.605118729533963	19.396670723375212
100-101	19.61259696867625	30.449961317630702	30.951422224341336	18.986019489351712
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	62.71267210061032
2	45.97366578280303
3	47.42193069296269
4	49.47049821947257
5	44.919237949038965
6	39.008078316761505
7	33.69918913437187
8	38.29148525670226
9	38.72993157665767
10-14	38.214953722315514
15-19	39.517361262609526
20-24	39.100361222388266
25-29	39.68327415223089
30-34	39.11283165568818
35-39	39.18765420105812
40-44	39.08729608923078
45-49	38.978209487566915
50-54	38.82734367403422
55-59	38.51880909889359
60-64	38.705727073229376
65-69	38.59013719058307
70-74	38.302796767373366
75-79	38.95918961453456
80-84	38.315017260204904
85-89	38.647126709116286
90-94	38.637488724831165
95-99	38.76280281773305
100-101	38.59861645802796
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	276.0
1	175.5
2	73.5
3	94.0
4	103.5
5	96.0
6	118.0
7	154.0
8	201.0
9	277.0
10	334.0
11	414.5
12	636.0
13	902.0
14	1161.0
15	1649.5
16	3027.5
17	4077.5
18	5942.0
19	10706.0
20	15792.5
21	21662.5
22	31088.5
23	45737.5
24	67788.5
25	92482.5
26	123382.5
27	158072.0
28	196441.0
29	244586.5
30	298738.0
31	349114.0
32	390725.0
33	426917.0
34	456500.0
35	461753.0
36	445683.0
37	439436.0
38	431760.0
39	427940.5
40	426479.0
41	397281.0
42	360121.5
43	330987.5
44	333055.5
45	310702.5
46	280710.5
47	272010.5
48	233944.5
49	204975.0
50	187096.5
51	165778.5
52	168722.0
53	160316.0
54	126121.5
55	106637.0
56	90752.0
57	76818.0
58	65362.0
59	57138.0
60	49811.5
61	41242.0
62	33391.0
63	27641.5
64	21599.0
65	15926.0
66	12398.0
67	9703.5
68	7293.0
69	5383.0
70	4088.0
71	3165.5
72	2403.0
73	1744.0
74	1355.0
75	1229.5
76	976.0
77	666.5
78	493.0
79	405.5
80	314.5
81	203.5
82	121.0
83	60.5
84	35.0
85	21.0
86	12.0
87	9.0
88	5.0
89	3.0
90	2.5
91	1.0
92	1.0
93	1.0
94	0.5
95	1.0
96	1.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03219655236446251
2	0.22388908308217803
3	0.01917437991132003
4	0.027449098942568837
5	0.04757707100990639
6	0.7394440680774568
7	0.0031068647663796624
8	0.08063493241851376
9	0.01877448642653849
10-14	0.027830535804975843
15-19	0.028406792570225138
20-24	0.013522551993074255
25-29	0.0195394108871719
30-34	0.02463343866254291
35-39	0.020665264852018392
40-44	0.017451761823030317
45-49	0.011992703071807437
50-54	0.011127292556023795
55-59	0.010009641534454874
60-64	0.014246461737319813
65-69	0.008405966123689927
70-74	0.01505035017852168
75-79	0.010563340205690852
80-84	0.014152127889627757
85-89	0.012733018702608135
90-94	0.006527492113126381
95-99	0.01038902766104249
100-101	9.433384769205576E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	9752597.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	86.0579111234629
#Duplication Level	Relative count
1	100.0
2	88.51076123068034
3	80.77711974577495
4	61.03712263469594
5	42.66069622995811
6	28.675429726996967
7	20.35533728152535
8	14.981944243824932
9	11.916799075545283
10++	128.8689874331937
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATA	28097	0.28809762158735774	No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCT	25507	0.2615405927262246	No Hit
GATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGA	24623	0.2524763404045097	No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTT	20542	0.2106310760098054	No Hit
TCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGC	19098	0.1958247633937914	No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAG	18493	0.18962128754012905	No Hit
CTCCTGTTCACTTTTCTTCCCCAATTCGAGGCTCTACACGCAAATGGCTG	17594	0.1804032300319597	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	745270	2.8104672	7.7699733	1
CCCCC	608260	2.0847485	13.173693	1
CTGCA	1165820	1.7453802	7.803006	2
GCAGG	724645	1.7259095	9.699956	4
GGCCC	480075	1.7095181	22.517588	9
TGCAG	1117595	1.7054662	6.694987	3
CCCCA	721290	1.6221921	10.841608	1
TGGCC	685540	1.5942758	15.6607065	8
GGCCG	433000	1.5716381	5.5508776	25-29
GCCCA	670460	1.53697	5.74557	1
CGGCC	430040	1.5313464	5.44718	25-29
TCTGC	1010825	1.5061654	6.1648192	1
CCTGG	642350	1.4938343	5.15015	1
CCCAG	651540	1.4935976	6.7254605	1
CCTCC	663375	1.4848739	6.169593	1
CCCAA	1004505	1.4824243	5.3233185	2
CCCTG	637130	1.4536458	6.8589196	1
CTGGA	952205	1.4530786	10.816375	2
ACTGG	925935	1.4129902	9.480905	1
CAGGA	918890	1.4089129	6.1767874	5
ATGGC	916785	1.3990272	10.434405	7
CGGGG	374085	1.3839968	5.895139	10-14
CCAGT	919280	1.3762785	5.636821	3
GCCCC	393635	1.3751758	7.655118	1
CCCCT	614265	1.3749479	9.563521	1
GCCGC	384955	1.3708014	5.1961594	25-29
CTCCA	929970	1.3659267	5.4162126	1
TGCAT	1396665	1.3655792	6.7993493	7
ATGCA	1376395	1.352165	7.5492616	6
CTCCC	569650	1.2750833	8.459787	1
CCTGT	825580	1.2301435	7.3892074	3
CTCCT	819420	1.1978518	8.670532	1
CCGGG	329880	1.1973487	5.1415524	10-14
CCCGG	334970	1.1928079	5.506655	1
TCCTG	795615	1.1854947	6.5780582	2
GATGC	776365	1.1847442	9.780163	5
GCATC	777330	1.1637616	9.029988	8
CATCT	1179495	1.1314117	6.2666473	9
GGATG	700280	1.0892572	10.225376	4
GCCCG	304475	1.0842171	5.0683937	10-14
CCCCG	308485	1.0777017	7.656816	1
CCTCG	436625	0.99618304	5.341126	1
TGGAT	963890	0.96062124	7.2262664	3
GATCC	629515	0.9424637	7.5723352	1
TATGG	807735	0.80499583	6.4280624	6
GTATG	796780	0.794078	6.6271405	5
CTCCG	340940	0.7778726	5.0437803	1
CTAGT	543745	0.5316428	6.3062143	2
ACTAG	526980	0.51770306	6.161765	1
>>END_MODULE