##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	3291_5903_10011_H94MGADXX_HK_CF35_ACAGTG_R2.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	87027784	
Filtered Sequences	0	
Sequence length	101	
%GC	38	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.042799986726077	34.0	31.0	34.0	28.0	34.0
2	31.18401594598801	34.0	31.0	34.0	28.0	34.0
3	31.256127020308824	34.0	31.0	34.0	28.0	34.0
4	34.523349290382946	37.0	35.0	37.0	32.0	37.0
5	34.498965847504515	37.0	35.0	37.0	32.0	37.0
6	34.47717084235995	37.0	35.0	37.0	32.0	37.0
7	34.46294971729948	37.0	35.0	37.0	32.0	37.0
8	34.43943465227151	37.0	35.0	37.0	32.0	37.0
9	36.105415013210035	39.0	37.0	39.0	32.0	39.0
10-14	36.35705329001598	39.4	37.2	39.4	32.0	39.4
15-19	37.39777674679158	41.0	38.2	41.0	32.0	41.0
20-24	37.34918528776971	41.0	38.8	41.0	31.6	41.0
25-29	37.21072041774613	41.0	38.0	41.0	31.0	41.0
30-34	37.059988313617175	41.0	38.0	41.0	30.4	41.0
35-39	36.771276632759026	40.0	38.0	41.0	30.0	41.0
40-44	36.644256884674896	40.0	38.0	41.0	29.8	41.0
45-49	36.280664478369346	40.0	37.2	41.0	28.2	41.0
50-54	35.973690797412466	40.0	36.6	41.0	27.6	41.0
55-59	35.6258812289188	39.8	35.6	41.0	27.0	41.0
60-64	35.07192366060936	39.0	35.0	41.0	26.0	41.0
65-69	34.423602175139834	37.6	34.6	40.2	25.6	41.0
70-74	33.64842335178844	36.4	34.0	39.2	24.6	41.0
75-79	32.72945784532443	35.2	33.8	37.8	22.2	39.6
80-84	31.882454598637146	35.0	33.0	36.4	20.2	38.4
85-89	31.449472386887386	35.0	33.0	35.8	19.8	36.8
90-94	31.01771658577449	35.0	33.0	35.0	17.8	36.0
95-99	30.9166618605387	35.0	33.8	35.0	8.0	36.0
100-101	30.547426451763954	35.0	33.0	35.0	2.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2919310.0
3	395118.0
4	240227.0
5	182628.0
6	165963.0
7	161753.0
8	168357.0
9	181194.0
10	187227.0
11	195875.0
12	198683.0
13	199508.0
14	201992.0
15	204389.0
16	204017.0
17	202877.0
18	204706.0
19	206382.0
20	208197.0
21	214988.0
22	232547.0
23	275310.0
24	325877.0
25	382977.0
26	459699.0
27	552756.0
28	667043.0
29	804506.0
30	981756.0
31	1229629.0
32	1537076.0
33	1966622.0
34	2738320.0
35	4140315.0
36	7619467.0
37	1.6842821E7
38	2.8374138E7
39	1.1025346E7
40	28188.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.28645016757028	18.8210702433687	15.228460418722303	38.66401917033872
2	20.678668987743308	21.280479400835915	33.3879807330302	24.65287087839058
3	20.33824685296093	22.414515380741364	28.42335560979344	28.823882156504272
4	23.297871684518846	25.77232721018695	23.978621325595697	26.95117977969851
5	24.58652553198671	30.323551290616557	25.69238208695251	19.39754109044422
6	21.228193815323102	35.57253057712115	25.718347133427184	17.480928474128568
7	16.722855942644482	24.594939612530915	42.112849055882165	16.569355388942437
8	17.612955675154296	27.336169769838875	34.71576457318434	20.335109981822487
9	18.040468739697477	25.478917577950348	36.47271630013729	20.00789738221488
10-14	18.949192568593702	32.39587517372776	30.143802175853914	18.511130081824625
15-19	19.426091388861376	30.729312500054217	30.542007163628032	19.302588947456375
20-24	19.136823208117374	30.75612848550881	30.564420800817558	19.542627505556258
25-29	19.271684480547428	30.614414660708846	30.426618665904677	19.68728219283905
30-34	18.99711328387638	30.93611505878001	30.68276985195284	19.384001805390767
35-39	19.198162424593367	31.029181505863423	30.36621719411084	19.406438875432368
40-44	19.1609259422787	30.863813121517676	30.646566837014394	19.328694099189235
45-49	19.319083705728485	30.878273966399085	30.59483850224833	19.207803825624104
50-54	19.068834111220756	31.01615004528508	30.726251781044905	19.18876406244926
55-59	19.02618667203998	30.90754057722391	30.915622457208936	19.15065029352718
60-64	19.182932188737556	31.092846014348552	30.642137776408955	19.08208402050494
65-69	19.092250778374254	30.888687232096533	30.91034079819735	19.108721191331863
70-74	19.08673373598593	30.996752249570637	30.79986442958223	19.116649584861204
75-79	19.183386641191884	30.988132438226117	30.517546958948856	19.31093396163314
80-84	19.19478963982004	30.921279296943155	30.73661414536032	19.147316917876488
85-89	19.280147201103848	30.929680764332474	30.76140502672357	19.028767007840106
90-94	19.219671391754105	30.80764328913075	30.73390551353288	19.23877980558227
95-99	19.384294238255237	31.009430604219446	30.50597550738578	19.100299650139533
100-101	19.70314582927758	30.75060017887296	30.630090397953737	18.916163593895725
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.950469337909
2	45.33153986613389
3	49.1621290094652
4	50.24905146421735
5	43.98406662243094
6	38.70912228945167
7	33.29221133158692
8	37.94806565697679
9	38.04836612191235
10-14	37.46032265041833
15-19	38.72868033631776
20-24	38.67945071367363
25-29	38.95896667338648
30-34	38.38111508926715
35-39	38.604601300025735
40-44	38.48962004146793
45-49	38.526887531352585
50-54	38.25759817367002
55-59	38.176836965567155
60-64	38.265016209242496
65-69	38.20097196970612
70-74	38.20338332084713
75-79	38.49432060282502
80-84	38.342106557696525
85-89	38.30891420894395
90-94	38.45845119733637
95-99	38.48459388839477
100-101	38.619309423173306
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	31652.0
1	19828.0
2	7261.5
3	6790.5
4	7107.0
5	7189.5
6	7127.5
7	7081.5
8	7190.0
9	7351.0
10	7663.5
11	8570.0
12	9838.0
13	11400.5
14	13527.5
15	16758.5
16	26412.5
17	36115.5
18	54981.5
19	92803.0
20	132616.5
21	184263.5
22	264963.5
23	396570.5
24	590623.5
25	799951.5
26	1065000.5
27	1385358.0
28	1747680.0
29	2180211.5
30	2688668.0
31	3187617.0
32	3628006.0
33	3917043.0
34	4053295.0
35	4044106.0
36	3995786.0
37	4102776.0
38	4135784.5
39	4156979.5
40	4113013.5
41	3816409.0
42	3483562.5
43	3172438.0
44	2990017.5
45	2788736.5
46	2559418.5
47	2331549.5
48	2002063.0
49	1782397.0
50	1585584.0
51	1379517.5
52	1271389.0
53	1137795.0
54	964705.0
55	826335.0
56	700812.5
57	591939.5
58	487014.0
59	416724.0
60	356462.5
61	288435.5
62	226876.0
63	178860.0
64	137230.0
65	98944.5
66	71724.5
67	53591.5
68	40739.0
69	30807.5
70	23540.5
71	18446.0
72	14159.0
73	10535.5
74	8540.5
75	8125.5
76	7237.0
77	5169.5
78	3942.0
79	3926.5
80	3286.0
81	2057.5
82	1325.5
83	857.5
84	577.0
85	356.0
86	255.0
87	195.0
88	147.5
89	117.0
90	112.5
91	110.5
92	89.0
93	81.5
94	69.5
95	71.0
96	78.0
97	77.5
98	99.0
99	126.0
100	464.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34333862850052577
2	0.10601901572031296
3	0.10080573808474774
4	0.10656366936793427
5	0.11787729766852388
6	0.14445731491910674
7	0.1617885616850821
8	0.16046944272417646
9	0.1268273129877695
10-14	0.12660784284706134
15-19	0.1247079898070253
20-24	0.1354298530685327
25-29	0.12377587369109617
30-34	0.15934244631576508
35-39	0.2959199788426188
40-44	0.16376792956143754
45-49	0.2385410617832117
50-54	0.34509025301620916
55-59	0.14617308881494673
60-64	0.10998119864800877
65-69	0.11047575335251555
70-74	0.10639613666366594
75-79	0.1140582874085361
80-84	0.12406773450648817
85-89	0.11747995329859257
90-94	0.1348743982726252
95-99	0.12388894103060237
100-101	0.12537720137743597
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	8.7027784E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	86.30269457145927
#Duplication Level	Relative count
1	100.0
2	59.708300647897964
3	45.300560736802346
4	31.03808942735117
5	21.979139428803858
6	17.25790987535954
7	13.361805979255644
8	11.723176152706355
9	9.831778959295738
10++	270.8736453702897
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCTGTTCACTTTTCTTCCCCAATTCGAGGCTCTACACGCAAATGGCTG	126433	0.145278891623852	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	7317020	3.1978073	8.437831	1
CCCCC	7584170	3.110285	15.490373	1
CCCCA	6830630	1.7998564	12.286576	1
CCCAA	9225000	1.5618109	5.4666486	2
CTGGG	5689525	1.5567412	6.193265	1
CCTGG	5697110	1.5391108	5.718876	1
CCCCT	5813455	1.5310848	9.901432	1
CCCAG	5504070	1.4688797	7.130375	1
CCTCC	5574025	1.4680262	6.740666	1
CCTGC	5477465	1.4610655	5.4935107	1
GCCCA	5423745	1.4474434	5.85988	1
TGGCC	5352285	1.4459541	5.6125326	8
GGCCC	3430065	1.4429286	6.8343697	9
CCCTG	5393215	1.4385927	6.4980407	1
CTGGA	8040050	1.3955936	5.3872013	2
GCCCC	3357540	1.3945642	8.682204	1
CTCCC	5268645	1.3875985	9.461201	1
CTCCA	8042625	1.3609673	6.613646	1
CCCAC	5097260	1.3431171	5.840041	1
CCAGT	7572750	1.2978626	5.8621826	3
CGGGG	2978245	1.2851485	6.771689	1
TCCTG	7374255	1.2632259	6.491468	2
CTCCT	7395755	1.250893	8.536972	1
CCTGT	7238930	1.2400445	6.871448	3
CCCCG	2829990	1.1754447	8.711242	1
CCCGG	2554030	1.0744063	6.4261265	1
CCGGG	2519705	1.073538	5.3494463	1
CTCGG	3698750	0.99924105	6.7180367	1
CCTCG	3670200	0.97899354	5.549066	1
CCCGC	2028085	0.8423711	5.336442	1
CTCCG	3094395	0.8254026	5.8122096	1
>>END_MODULE