FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | 3291_5903_10011_H94MGADXX_HK_CF35_ACAGTG_R2.fastq |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 87027784 |
| Filtered Sequences | 0 |
| Sequence length | 101 |
| %GC | 38 |
Per base sequence quality
Per sequence quality scores
![[FAIL]](Icons/error.png)
Per base sequence content
Per base GC content
![[WARN]](Icons/warning.png)
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| CTCCTGTTCACTTTTCTTCCCCAATTCGAGGCTCTACACGCAAATGGCTG | 126433 | 0.145278891623852 | No Hit |
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| GGGGG | 7317020 | 3.1978073 | 8.437831 | 1 |
| CCCCC | 7584170 | 3.110285 | 15.490373 | 1 |
| CCCCA | 6830630 | 1.7998564 | 12.286576 | 1 |
| CCCAA | 9225000 | 1.5618109 | 5.4666486 | 2 |
| CTGGG | 5689525 | 1.5567412 | 6.193265 | 1 |
| CCTGG | 5697110 | 1.5391108 | 5.718876 | 1 |
| CCCCT | 5813455 | 1.5310848 | 9.901432 | 1 |
| CCCAG | 5504070 | 1.4688797 | 7.130375 | 1 |
| CCTCC | 5574025 | 1.4680262 | 6.740666 | 1 |
| CCTGC | 5477465 | 1.4610655 | 5.4935107 | 1 |
| GCCCA | 5423745 | 1.4474434 | 5.85988 | 1 |
| TGGCC | 5352285 | 1.4459541 | 5.6125326 | 8 |
| GGCCC | 3430065 | 1.4429286 | 6.8343697 | 9 |
| CCCTG | 5393215 | 1.4385927 | 6.4980407 | 1 |
| CTGGA | 8040050 | 1.3955936 | 5.3872013 | 2 |
| GCCCC | 3357540 | 1.3945642 | 8.682204 | 1 |
| CTCCC | 5268645 | 1.3875985 | 9.461201 | 1 |
| CTCCA | 8042625 | 1.3609673 | 6.613646 | 1 |
| CCCAC | 5097260 | 1.3431171 | 5.840041 | 1 |
| CCAGT | 7572750 | 1.2978626 | 5.8621826 | 3 |
| CGGGG | 2978245 | 1.2851485 | 6.771689 | 1 |
| TCCTG | 7374255 | 1.2632259 | 6.491468 | 2 |
| CTCCT | 7395755 | 1.250893 | 8.536972 | 1 |
| CCTGT | 7238930 | 1.2400445 | 6.871448 | 3 |
| CCCCG | 2829990 | 1.1754447 | 8.711242 | 1 |
| CCCGG | 2554030 | 1.0744063 | 6.4261265 | 1 |
| CCGGG | 2519705 | 1.073538 | 5.3494463 | 1 |
| CTCGG | 3698750 | 0.99924105 | 6.7180367 | 1 |
| CCTCG | 3670200 | 0.97899354 | 5.549066 | 1 |
| CCCGC | 2028085 | 0.8423711 | 5.336442 | 1 |
| CTCCG | 3094395 | 0.8254026 | 5.8122096 | 1 |