[2014-12-19 11:32:49] Beginning TopHat run (v2.0.9) ----------------------------------------------- [2014-12-19 11:32:49] Checking for Bowtie Bowtie version: 2.1.0.0 [2014-12-19 11:32:49] Checking for Samtools Samtools version: 0.1.18.0 [2014-12-19 11:32:49] Checking for Bowtie index files (genome).. [2014-12-19 11:32:49] Checking for reference FASTA file [2014-12-19 11:32:49] Generating SAM header for Crassostrea_gigas.GCA_000297895.1.24.dna_sm.toplevel format: fastq quality scale: phred33 (default) [2014-12-19 11:32:50] Reading known junctions from GTF file [2014-12-19 11:32:52] Preparing reads left reads: min. length=101, max. length=101, 23807053 kept reads (88171 discarded) [2014-12-19 11:40:33] Building transcriptome data files.. [2014-12-19 11:40:37] Building Bowtie index from Crassostrea_gigas.GCA_000297895.1.24.fa [2014-12-19 11:41:20] Mapping left_kept_reads to transcriptome Crassostrea_gigas.GCA_000297895.1.24 with Bowtie2 /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 11:48:43] Resuming TopHat pipeline with unmapped reads [2014-12-19 11:48:43] Mapping left_kept_reads.m2g_um to genome Crassostrea_gigas.GCA_000297895.1.24.dna_sm.toplevel with Bowtie2 /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx) [2014-12-19 12:13:31] Mapping left_kept_reads.m2g_um_seg1 to genome Crassostrea_gigas.GCA_000297895.1.24.dna_sm.toplevel with Bowtie2 (1/5) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 12:16:40] Mapping left_kept_reads.m2g_um_seg2 to genome Crassostrea_gigas.GCA_000297895.1.24.dna_sm.toplevel with Bowtie2 (2/5) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 12:19:53] Mapping left_kept_reads.m2g_um_seg3 to genome Crassostrea_gigas.GCA_000297895.1.24.dna_sm.toplevel with Bowtie2 (3/5) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 12:23:03] Mapping left_kept_reads.m2g_um_seg4 to genome Crassostrea_gigas.GCA_000297895.1.24.dna_sm.toplevel with Bowtie2 (4/5) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 12:26:12] Mapping left_kept_reads.m2g_um_seg5 to genome Crassostrea_gigas.GCA_000297895.1.24.dna_sm.toplevel with Bowtie2 (5/5) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 12:29:20] Searching for junctions via segment mapping [2014-12-19 12:32:29] Retrieving sequences for splices [2014-12-19 12:32:51] Indexing splices [2014-12-19 12:33:47] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/5) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 12:35:10] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/5) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 12:36:36] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/5) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 12:38:03] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/5) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 12:39:26] Mapping left_kept_reads.m2g_um_seg5 to genome segment_juncs with Bowtie2 (5/5) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 12:40:51] Joining segment hits [2014-12-19 12:44:11] Reporting output tracks ----------------------------------------------- [2014-12-19 12:55:02] A summary of the alignment counts can be found in ./tophat_out/align_summary.txt [2014-12-19 12:55:02] Run complete: 01:22:12 elapsed