[2014-12-19 15:34:04] Beginning TopHat run (v2.0.9) ----------------------------------------------- [2014-12-19 15:34:04] Checking for Bowtie Bowtie version: 2.1.0.0 [2014-12-19 15:34:04] Checking for Samtools Samtools version: 0.1.18.0 [2014-12-19 15:34:04] Checking for Bowtie index files (genome).. [2014-12-19 15:34:04] Checking for reference FASTA file [2014-12-19 15:34:04] Generating SAM header for Crassostrea_gigas.GCA_000297895.1.24.dna_sm.toplevel format: fastq quality scale: phred33 (default) [2014-12-19 15:34:06] Reading known junctions from GTF file [2014-12-19 15:34:08] Preparing reads left reads: min. length=101, max. length=101, 18099982 kept reads (864606 discarded) [2014-12-19 15:39:56] Building transcriptome data files.. [2014-12-19 15:40:00] Building Bowtie index from Crassostrea_gigas.GCA_000297895.1.24.fa [2014-12-19 15:40:45] Mapping left_kept_reads to transcriptome Crassostrea_gigas.GCA_000297895.1.24 with Bowtie2 /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 15:45:58] Resuming TopHat pipeline with unmapped reads [2014-12-19 15:45:58] Mapping left_kept_reads.m2g_um to genome Crassostrea_gigas.GCA_000297895.1.24.dna_sm.toplevel with Bowtie2 /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/bam2fastx) [2014-12-19 16:20:02] Mapping left_kept_reads.m2g_um_seg1 to genome Crassostrea_gigas.GCA_000297895.1.24.dna_sm.toplevel with Bowtie2 (1/5) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 16:24:06] Mapping left_kept_reads.m2g_um_seg2 to genome Crassostrea_gigas.GCA_000297895.1.24.dna_sm.toplevel with Bowtie2 (2/5) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 16:28:03] Mapping left_kept_reads.m2g_um_seg3 to genome Crassostrea_gigas.GCA_000297895.1.24.dna_sm.toplevel with Bowtie2 (3/5) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 16:31:44] Mapping left_kept_reads.m2g_um_seg4 to genome Crassostrea_gigas.GCA_000297895.1.24.dna_sm.toplevel with Bowtie2 (4/5) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 16:35:21] Mapping left_kept_reads.m2g_um_seg5 to genome Crassostrea_gigas.GCA_000297895.1.24.dna_sm.toplevel with Bowtie2 (5/5) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 16:38:45] Searching for junctions via segment mapping [2014-12-19 16:41:32] Retrieving sequences for splices [2014-12-19 16:41:53] Indexing splices [2014-12-19 16:42:24] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/5) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 16:43:22] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/5) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 16:44:21] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/5) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 16:45:20] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/5) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 16:46:18] Mapping left_kept_reads.m2g_um_seg5 to genome segment_juncs with Bowtie2 (5/5) /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering: /lib64/libz.so.1: no version information available (required by /usr/local2/tophat-2.0.9.Linux_x86_64/fix_map_ordering) [2014-12-19 16:47:12] Joining segment hits [2014-12-19 16:50:05] Reporting output tracks ----------------------------------------------- [2014-12-19 16:58:18] A summary of the alignment counts can be found in ./tophat_out/align_summary.txt [2014-12-19 16:58:18] Run complete: 01:24:13 elapsed