Basic Statistics
Measure | Value |
---|---|
Filename | filtered_Bs_CgLarve_T3D3_GCCAAT_L007_R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 5837666 |
Filtered Sequences | 0 |
Sequence length | 76 |
%GC | 17 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per base GC content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC | 42626 | 0.7301890858435546 | TruSeq Adapter, Index 6 (100% over 50bp) |
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC | 20787 | 0.3560840925123157 | TruSeq Adapter, Index 6 (100% over 50bp) |
Kmer Content
Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCCCC | 2020 | 205048.28 | 437974.47 | 15 |
CACGC | 83030 | 7567.283 | 350975.56 | 31 |
ACGCC | 83010 | 7565.4604 | 351407.6 | 32 |
CGCCA | 82930 | 7558.169 | 351730.75 | 33 |
CCGTC | 96445 | 7232.9795 | 351213.06 | 50 |
CACAC | 103540 | 4243.1177 | 190592.64 | 12 |
ACTCC | 96060 | 3239.3074 | 154771.52 | 23 |
CTCCA | 95720 | 3227.8416 | 154466.08 | 24 |
ACCCC | 390 | 795.43634 | 10273.899 | 31 |
CACCC | 385 | 785.23846 | 9539.297 | 30 |
CCCCA | 365 | 744.44684 | 11007.928 | 32 |
CCGCC | 115 | 521.63495 | 9805.915 | 49 |
CTCCC | 240 | 402.7956 | 9658.6875 | 24 |
CCCAC | 180 | 367.12448 | 2939.6152 | 54 |
CGCCC | 80 | 362.8765 | 11424.585 | 32 |
CCACC | 175 | 356.92657 | 2938.46 | 55 |
TGCCG | 97900 | 328.08344 | 15938.422 | 48 |
GCCGT | 96945 | 324.88306 | 15824.858 | 49 |
CCGGC | 1310 | 265.52426 | 9932.071 | 49 |
CGTCT | 194195 | 240.79327 | 5806.452 | 51 |
CCCGC | 50 | 226.7978 | 8159.6406 | 30 |
CGCGC | 1095 | 221.94585 | 1969.0789 | 31 |
AGCAC | 104540 | 191.43594 | 8668.773 | 10 |
GCACA | 102415 | 187.5446 | 8647.046 | 11 |
ACACG | 99725 | 182.6186 | 8583.837 | 13 |
GCCCC | 40 | 181.43825 | 3271.6147 | 65 |
CCCCT | 95 | 159.43993 | 3026.212 | 67 |
ACGTC | 102950 | 155.1315 | 7015.185 | 15 |
GCCAA | 83275 | 152.49501 | 7100.8394 | 34 |
CACGT | 100475 | 151.40202 | 7034.148 | 14 |
ATGCC | 98285 | 148.10199 | 7163.8174 | 47 |
GTCAC | 97505 | 146.92662 | 7018.357 | 29 |
TCCAG | 97020 | 146.1958 | 6997.486 | 25 |
CAGTC | 96875 | 145.97731 | 6988.723 | 27 |
CCAGT | 96775 | 145.82663 | 6980.326 | 26 |
TCACG | 87725 | 132.18951 | 6129.8975 | 30 |
CCCTC | 75 | 125.87363 | 4232.915 | 49 |
TCTGC | 97835 | 121.31109 | 5886.237 | 56 |
CTGCT | 97725 | 121.1747 | 5887.0005 | 57 |
TCTCG | 97475 | 120.864716 | 5851.2944 | 41 |
CTCGT | 97215 | 120.54232 | 5837.4756 | 42 |
CACCA | 2825 | 115.76983 | 6826.913 | 31 |
CCTCC | 55 | 92.30733 | 2414.5166 | 23 |
TCACC | 2710 | 91.385826 | 5702.163 | 30 |
CCCCG | 15 | 68.039345 | 1635.7047 | 70 |
TCCCC | 40 | 67.1326 | 1210.5056 | 65 |
AACTC | 97535 | 66.085495 | 3153.6018 | 22 |
CCAAT | 85510 | 57.937874 | 2626.641 | 35 |
ATCTC | 97460 | 54.33813 | 2629.6023 | 40 |
CGGCG | 5720 | 51.807545 | 84.936226 | 72 |
CGCGG | 5675 | 51.399967 | 81.41412 | 9 |
TCGCG | 14520 | 48.65957 | 85.60363 | 30 |
GCGCG | 5070 | 45.920322 | 81.44447 | 24 |
CTTCT | 97440 | 44.70419 | 2164.5764 | 54 |
TCTTC | 96625 | 44.330276 | 2148.0305 | 53 |
CGTCG | 12800 | 42.895493 | 91.770966 | 50 |
CGCGA | 10005 | 40.746105 | 66.04027 | 47 |
CGCCG | 190 | 38.51115 | 1093.9506 | 32 |
CGCAC | 415 | 37.822742 | 1081.3883 | 12 |
CGACG | 9155 | 37.284412 | 61.50158 | 19 |
GCGCC | 175 | 35.4708 | 1823.2509 | 32 |
CGCGT | 10465 | 35.07041 | 67.476494 | 14 |
ACGCG | 7645 | 31.134827 | 73.21529 | 13 |
CGGAC | 6300 | 25.657217 | 39.53626 | 3 |
CTCCG | 300 | 22.498772 | 944.12665 | 24 |
CCACG | 225 | 20.506308 | 327.69342 | 12 |
CGTCC | 270 | 20.248896 | 539.30316 | 16 |
CCCAG | 220 | 20.050611 | 458.96024 | 25 |
ACACC | 455 | 18.646112 | 176.81575 | 12 |
CCCAA | 430 | 17.621603 | 235.9419 | 33 |
GCCAC | 190 | 17.316437 | 458.77078 | 11 |
CACCG | 180 | 16.405046 | 327.69342 | 14 |
GCACC | 170 | 15.493654 | 426.00143 | 11 |
GCCGC | 75 | 15.201771 | 438.2146 | 48 |
CGGAA | 171390 | 14.024603 | 395.4572 | 4 |
ATCGG | 202360 | 13.625811 | 330.1319 | 2 |
AACCC | 325 | 13.318651 | 191.58337 | 21 |
GCTCC | 175 | 13.124284 | 593.4129 | 23 |
TCGGA | 187080 | 12.596939 | 326.3793 | 3 |
GCCGG | 1360 | 12.317878 | 440.58878 | 48 |
GCGGC | 1360 | 12.317878 | 32.575695 | 23 |
CCGCG | 60 | 12.161416 | 73.10642 | 72 |
CTTCC | 435 | 12.070672 | 260.1785 | 66 |
ACCCA | 285 | 11.679434 | 103.173546 | 22 |
CCAAC | 285 | 11.679433 | 147.66125 | 54 |
CGGGC | 1270 | 11.502724 | 26.073246 | 33 |
TCGGC | 3425 | 11.477894 | 56.63205 | 3 |
GATCG | 168390 | 11.338459 | 328.37793 | 1 |
ACCAC | 275 | 11.269629 | 132.61182 | 13 |
GCGTC | 3355 | 11.24331 | 55.427116 | 15 |
CCACA | 270 | 11.064727 | 88.40788 | 12 |
CCAGC | 120 | 10.936697 | 295.0537 | 26 |
CAACC | 255 | 10.450019 | 132.86913 | 43 |
CGATC | 6915 | 10.419954 | 251.05148 | 33 |
AGAGC | 124130 | 10.157384 | 395.2218 | 8 |
CGGCC | 50 | 10.134514 | 218.80756 | 33 |
CGGTC | 2920 | 9.785533 | 37.373802 | 27 |
TTCGC | 7890 | 9.783253 | 16.528673 | 45 |
CCGAC | 105 | 9.56961 | 361.2332 | 50 |
GAGCA | 112465 | 9.202852 | 393.5742 | 9 |
GCCCA | 100 | 9.1139145 | 229.56786 | 33 |
GGGGG | 503925 | 9.113624 | 10.186433 | 62 |
ACGGC | 2230 | 9.08184 | 111.28724 | 15 |
CGCCT | 120 | 8.999509 | 162.13576 | 50 |
CAGCC | 95 | 8.658218 | 196.72523 | 27 |
CGCTC | 115 | 8.624529 | 134.82497 | 2 |
TCCCG | 110 | 8.24955 | 134.90678 | 29 |
GCCCG | 40 | 8.107611 | 146.07153 | 48 |
CGAGC | 1975 | 8.043333 | 29.286116 | 7 |
CCCGT | 105 | 7.8745704 | 80.93055 | 26 |
TCGTC | 6330 | 7.8489213 | 27.700592 | 52 |
GTCGC | 2255 | 7.556978 | 44.60973 | 29 |
CGTTC | 6045 | 7.4955344 | 11.61626 | 53 |
ACGAC | 4090 | 7.489698 | 12.524763 | 39 |
AGCGC | 1810 | 7.3713584 | 55.64362 | 10 |
GGCGC | 810 | 7.336383 | 22.83568 | 55 |
CGGCT | 2130 | 7.1380773 | 164.22171 | 50 |
ATCGC | 4715 | 7.104856 | 17.363224 | 40 |
TACGC | 4705 | 7.0897875 | 88.37464 | 31 |
TGCGC | 2080 | 6.9705167 | 15.682196 | 37 |
CGGGG | 16120 | 6.5241823 | 9.02729 | 1 |
CGTAC | 4240 | 6.389097 | 11.400446 | 44 |
TCGAC | 4150 | 6.253479 | 14.086885 | 18 |
GAGAG | 1661795 | 6.0764036 | 6.8469787 | 42 |
TCCGC | 80 | 5.999672 | 81.03594 | 55 |
AGCCC | 65 | 5.924044 | 131.36879 | 47 |
TCCCA | 175 | 5.9012985 | 157.68669 | 25 |
GACGC | 1415 | 5.762692 | 29.304287 | 30 |
CACTC | 170 | 5.7326903 | 109.19897 | 31 |
AACGC | 3100 | 5.676788 | 15.806859 | 22 |
GTCTG | 100040 | 5.5429893 | 261.51935 | 17 |
GCTTG | 97830 | 5.4205384 | 262.15115 | 59 |
CACGA | 2810 | 5.145734 | 299.1323 | 31 |
GGCGG | 12570 | 5.087405 | 7.7157664 | 25 |
CCCGG | 25 | 5.067257 | 73.09336 | 68 |
GCGAC | 1230 | 5.0092664 | 11.739826 | 48 |
CGTGC | 1485 | 4.976547 | 13.293832 | 66 |
GGGCG | 12155 | 4.919444 | 7.8581014 | 2 |
GCGGG | 12115 | 4.903255 | 6.989032 | 28 |
TGCCC | 65 | 4.8747334 | 189.17462 | 47 |
CCAGA | 2650 | 4.8527384 | 307.06833 | 33 |
ACCAG | 2630 | 4.816114 | 309.6794 | 32 |
GCACG | 1135 | 4.622371 | 55.678146 | 30 |
CGACC | 50 | 4.5569572 | 65.73411 | 66 |
ACCCG | 50 | 4.5569572 | 65.67949 | 51 |
GACGG | 24730 | 4.500463 | 6.226825 | 42 |
CGAAC | 2450 | 4.4864936 | 11.851916 | 20 |
TCGTA | 174555 | 4.348852 | 119.82868 | 43 |
GGCCG | 475 | 4.3022 | 153.39885 | 47 |
CGTAT | 166245 | 4.1418176 | 119.4943 | 44 |
TCGGG | 27650 | 4.140577 | 10.8762665 | 3 |
GCCCT | 55 | 4.124775 | 162.1405 | 48 |
CTCGC | 55 | 4.1247745 | 80.95156 | 31 |
GCCTC | 55 | 4.1247745 | 54.04539 | 52 |
TCGCC | 55 | 4.1247745 | 107.93717 | 32 |
GACCA | 2230 | 4.083625 | 247.79218 | 35 |
CACTG | 2705 | 4.076063 | 200.06285 | 31 |
GGCCC | 20 | 4.0538054 | 73.099205 | 71 |
CTCAC | 115 | 3.8779964 | 60.709175 | 57 |
GTCCC | 50 | 3.749795 | 161.88383 | 28 |
TCCAC | 110 | 3.7093878 | 84.92583 | 30 |
CCCGA | 40 | 3.6455657 | 98.51839 | 52 |
CACGG | 870 | 3.5431392 | 114.21585 | 14 |
CCATC | 105 | 3.5407794 | 36.42491 | 58 |
CTGAC | 2325 | 3.5034552 | 204.41986 | 33 |
GGACG | 19165 | 3.4877224 | 4.842031 | 4 |
CCTCT | 125 | 3.4685838 | 79.98751 | 50 |
TCACA | 5110 | 3.4623146 | 187.70184 | 30 |
GTCGG | 23120 | 3.462211 | 5.653505 | 2 |
GGTCG | 22910 | 3.430764 | 5.4950366 | 1 |
CGAGG | 18755 | 3.4131088 | 4.5184584 | 33 |
CCGCT | 45 | 3.374816 | 81.01698 | 56 |
CACCT | 100 | 3.3721707 | 36.482018 | 69 |
TGACC | 2220 | 3.3452346 | 204.42126 | 34 |
TCTCC | 120 | 3.3298404 | 69.94344 | 40 |
TGAAC | 108290 | 3.2786725 | 143.67886 | 20 |
AGAGA | 1992375 | 3.2757614 | 3.6278718 | 43 |
CGGGA | 17965 | 3.2693415 | 10.403824 | 4 |
GGCAC | 800 | 3.2580593 | 71.794525 | 29 |
GCGAG | 17765 | 3.2329452 | 4.131201 | 48 |
CCACT | 95 | 3.2035623 | 24.266438 | 31 |
CGGAG | 17525 | 3.189269 | 7.8519435 | 3 |
ACGGA | 38530 | 3.1528556 | 4.1880984 | 60 |
TTCGG | 56650 | 3.1388478 | 3.8126996 | 44 |
GGCGA | 17080 | 3.1082857 | 4.9101977 | 1 |
TCGGT | 55910 | 3.097846 | 3.7254157 | 3 |
GAACT | 100275 | 3.0360045 | 143.46002 | 21 |
CTGAA | 100265 | 3.0357015 | 143.18665 | 19 |
CGGTA | 44930 | 3.0253398 | 3.7304661 | 1 |
TTTCG | 147280 | 3.0193868 | 3.5019195 | 21 |
AGTCA | 98745 | 2.9896812 | 142.05898 | 28 |
CCTTC | 105 | 2.9136105 | 39.993862 | 52 |
CCCAT | 85 | 2.8663452 | 84.94126 | 34 |
GAGCG | 15645 | 2.847139 | 5.0383296 | 9 |
AGATC | 92990 | 2.8154383 | 19.984049 | 72 |
ACACT | 4080 | 2.7644312 | 154.56403 | 32 |
ACCGC | 30 | 2.7341743 | 65.58559 | 32 |
TATGC | 108550 | 2.704408 | 118.35139 | 46 |
CCCTA | 80 | 2.6977365 | 36.481762 | 68 |
CTCCT | 95 | 2.6361237 | 39.967857 | 41 |
CGTCA | 1745 | 2.6294756 | 100.85438 | 33 |
CCCTG | 35 | 2.6248567 | 54.08945 | 68 |
CTTGA | 100500 | 2.5038507 | 116.15349 | 60 |
TCCCT | 90 | 2.4973803 | 40.027462 | 67 |
TCTGA | 99985 | 2.49102 | 117.43024 | 18 |
CAGGC | 605 | 2.4639072 | 71.790825 | 27 |
CACTT | 4270 | 2.3807082 | 126.59477 | 33 |
CCTAC | 70 | 2.3605196 | 36.42491 | 58 |
ACCTC | 70 | 2.3605196 | 48.51354 | 22 |
AGGCC | 560 | 2.2806413 | 68.96325 | 46 |
CTCGG | 680 | 2.2788227 | 84.490845 | 42 |
GACCC | 25 | 2.2784786 | 65.73281 | 64 |
CTGCC | 30 | 2.249877 | 81.016975 | 56 |
CCCTT | 80 | 2.2198937 | 30.019848 | 68 |
CATCC | 65 | 2.191911 | 24.321074 | 63 |
ACCAA | 2640 | 2.1737866 | 112.03758 | 36 |
CCAGG | 505 | 2.0566497 | 73.24747 | 26 |
CACAG | 1110 | 2.0326564 | 90.934395 | 33 |
TTCTG | 98975 | 2.029086 | 97.49885 | 55 |
TGCTT | 98945 | 2.0284712 | 97.27297 | 58 |
GTCTT | 97375 | 1.9962846 | 96.237404 | 52 |
TTCCC | 70 | 1.942407 | 30.020002 | 64 |
ACGCT | 1250 | 1.8835782 | 78.07458 | 32 |
CCTCG | 25 | 1.8748977 | 81.03429 | 42 |
CCTGC | 25 | 1.8748975 | 54.005154 | 58 |
CTACC | 55 | 1.8546939 | 60.74132 | 43 |
GCGCA | 450 | 1.8326583 | 51.250706 | 11 |
ACGAT | 59180 | 1.7917801 | 6.863155 | 32 |
CAATC | 2590 | 1.7548718 | 90.24328 | 38 |
GAAAA | 2366730 | 1.7496933 | 5.1154766 | 63 |
CCGTG | 520 | 1.7426292 | 83.31836 | 50 |
GGAAG | 476190 | 1.7412031 | 18.905617 | 5 |
CGGCA | 425 | 1.730844 | 54.179317 | 4 |
ACACA | 2100 | 1.7291485 | 40.88515 | 32 |
TCACT | 3095 | 1.7255952 | 84.44862 | 30 |
ATCCC | 50 | 1.6860853 | 24.321302 | 64 |
CCTCA | 50 | 1.6860852 | 36.448635 | 59 |
GCTTC | 1350 | 1.6739407 | 58.97546 | 52 |
CATCT | 2935 | 1.6363884 | 101.15417 | 39 |
CGCTA | 1065 | 1.6048084 | 75.36966 | 33 |
TCATC | 2805 | 1.5639079 | 94.52936 | 38 |
TCCTC | 55 | 1.5261769 | 59.99079 | 52 |
TACCC | 45 | 1.5174768 | 24.28367 | 57 |
CACAT | 2125 | 1.439808 | 49.454346 | 12 |
TCCGA | 945 | 1.423985 | 32.507805 | 2 |
CCGCA | 15 | 1.3670871 | 32.866463 | 69 |
ATATC | 120265 | 1.3472669 | 44.826042 | 38 |
GAAGA | 814145 | 1.3385757 | 9.053002 | 6 |
GCTCG | 385 | 1.290216 | 49.475956 | 41 |
TCCGG | 380 | 1.2734598 | 32.54674 | 25 |
GACAC | 670 | 1.2269187 | 19.094215 | 11 |
CAATA | 86925 | 1.183386 | 53.567398 | 36 |
GCCGA | 290 | 1.1810465 | 21.982061 | 33 |
GCCAG | 290 | 1.1810464 | 24.903479 | 25 |
ACCCT | 35 | 1.1802598 | 24.266832 | 32 |
AAGAG | 710540 | 1.1682335 | 8.834865 | 7 |
ACTTC | 2090 | 1.1652646 | 48.527775 | 23 |
CCGAT | 760 | 1.1452155 | 14.671868 | 69 |
CATAC | 1655 | 1.1213564 | 46.28732 | 12 |
AACAC | 1305 | 1.0745422 | 10.393475 | 71 |
CTTCA | 1925 | 1.0732701 | 47.72873 | 24 |
GTATG | 927840 | 1.0329505 | 6.194758 | 45 |
CCGAA | 525 | 0.9613915 | 38.84685 | 3 |
CCGGT | 280 | 0.9383388 | 31.342142 | 26 |
TTCCA | 1655 | 0.9227335 | 39.30601 | 24 |
ATTCC | 1645 | 0.91715807 | 39.303486 | 23 |
CTGCG | 270 | 0.90482664 | 33.78912 | 56 |
ACTCT | 1555 | 0.8669792 | 35.89451 | 23 |
AGCTC | 570 | 0.8589117 | 21.704033 | 40 |
GAGCC | 205 | 0.8348776 | 21.964586 | 9 |
CTCTA | 1490 | 0.8307389 | 35.696278 | 24 |
CATGC | 550 | 0.82877433 | 35.24142 | 31 |
TACAC | 1195 | 0.8096802 | 27.772392 | 12 |
TATCT | 87205 | 0.80387485 | 36.611336 | 39 |
TTCCG | 630 | 0.78117234 | 12.073298 | 67 |
CCGGA | 190 | 0.7737891 | 8.785835 | 4 |
CCGGG | 85 | 0.76986736 | 6.5158205 | 25 |
TGCCA | 510 | 0.76849985 | 35.78703 | 33 |
ATCCG | 480 | 0.723294 | 34.152214 | 1 |
CTCTC | 25 | 0.69371676 | 10.0068655 | 66 |
GGCCA | 170 | 0.6923376 | 16.118893 | 32 |
GTCCG | 205 | 0.686998 | 12.049517 | 17 |
GGAGC | 3425 | 0.623295 | 5.300061 | 7 |
GTGCC | 185 | 0.61997384 | 22.940668 | 46 |
AAAAC | 37375 | 0.61834425 | 5.1193657 | 71 |
GACTC | 395 | 0.59521073 | 18.966154 | 21 |
ACCGG | 135 | 0.5497975 | 5.8755918 | 72 |
CAGAC | 295 | 0.5402105 | 6.584212 | 12 |
GCTGC | 160 | 0.5361936 | 19.30807 | 56 |
CAACG | 290 | 0.5310544 | 5.925791 | 12 |
AAACC | 630 | 0.5187445 | 5.9391284 | 71 |
AAAGC | 14090 | 0.51842755 | 8.134246 | 71 |
CTTCG | 410 | 0.50838196 | 12.956597 | 53 |
CCAAG | 275 | 0.50358605 | 10.544348 | 35 |
CCGTT | 390 | 0.48358282 | 12.956696 | 50 |
CTTGC | 385 | 0.47738305 | 15.642199 | 60 |
AGCCA | 260 | 0.4761177 | 9.876318 | 10 |
ACTCG | 290 | 0.43699014 | 7.596411 | 40 |
GAATC | 14350 | 0.43447188 | 7.2149816 | 38 |
GCATC | 285 | 0.42945585 | 17.900084 | 38 |
ACGCA | 230 | 0.42118105 | 5.271139 | 32 |
GCTCT | 330 | 0.4091855 | 10.731507 | 63 |
CGCTG | 120 | 0.40214518 | 10.8445 | 16 |
ACCGT | 250 | 0.37671563 | 5.4179683 | 15 |
GAACC | 205 | 0.3754005 | 6.5853453 | 21 |
AGTCC | 245 | 0.36918133 | 7.047445 | 28 |
GCAAC | 200 | 0.36624438 | 12.510003 | 11 |
CCTGA | 235 | 0.35411265 | 8.127049 | 18 |
CTCGA | 225 | 0.33904403 | 5.427297 | 42 |
CGACT | 195 | 0.2938382 | 5.972569 | 51 |
TGCTC | 235 | 0.29138964 | 10.283715 | 62 |
CCGTA | 190 | 0.28630385 | 9.23023 | 50 |
CTCAG | 185 | 0.27876958 | 8.67198 | 24 |
TCCTG | 220 | 0.27279034 | 5.350022 | 17 |
CTGTC | 215 | 0.26659054 | 8.48887 | 50 |
GTCCT | 190 | 0.23559164 | 5.349989 | 16 |
GTCTC | 170 | 0.21079251 | 5.3578963 | 40 |
CAGAT | 6290 | 0.19044098 | 5.185944 | 34 |
GATCA | 6210 | 0.18801883 | 5.2095065 | 36 |
AAGCA | 4320 | 0.15895009 | 7.7634645 | 72 |
GCCAT | 90 | 0.13561763 | 7.04901 | 34 |
GATCT | 4895 | 0.12195373 | 5.4528165 | 39 |
ACTTG | 4690 | 0.11684637 | 5.7914324 | 34 |