FastQCFastQC Report
Mon 24 Mar 2014
BiGoRNA_GTGTCTAC_1.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename BiGoRNA_GTGTCTAC_1.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 25542180
Filtered Sequences 0
Sequence length 50
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTAGACACATCTCG 1187285 4.648330721966567 Illumina Paired End PCR Primer 2 (97% over 38bp)
ATAATAATCAATCAAAGAATATCTTAGACAGGTGAACGATTTGCTAAGCC 151893 0.5946751608515796 No Hit
GGCCATTATACTAAAGGTACTTTTTTTTAACGCTTAAAAACAATGCTTTC 135385 0.5300448121499417 No Hit
GGGAGGTGCTAATACAGCAAAATCTCTTGAAAGGCCATTATACTAAAGGT 128977 0.504956898745526 No Hit
CAGCATAATAATCAATCAAAGAATATCTTAGACAGGTGAACGATTTGCTA 125335 0.490698131482904 No Hit
CGGTTCAGCAGGAATGCCGAGACCGGTAGACACATCTCGTATGCCGTCTT 111407 0.4361687216987743 Illumina Paired End PCR Primer 2 (96% over 27bp)
CCCACATTAAAGCTGCACTTAAAAGCATTTTTCGAACAACCAGCTATCTT 106282 0.41610387210488686 No Hit
GTCCTTTCGTACATGCCGAGATAAGTTTATGTAAAGCAGATAGAAACCAA 97933 0.38341676395671787 No Hit
CCACATTAAAGCTGCACTTAAAAGCATTTTTCGAACAACCAGCTATCTTA 90547 0.3544998899858978 No Hit
ATTAAATTATCATTCTGCAATTTTTTTCTGGATTTACTCTTTCCAGTCTC 88941 0.34821225126437916 No Hit
CTAGTGAGTATCTTTAACACACTACGCCCCACATTAAAGCTGCACTTAAA 82509 0.32303037563747494 No Hit
ATTTTTTAGAAATTATTCTTTTACTATTAGAAAGACAAGTTGTTAAATAC 80452 0.31497703015169415 No Hit
GAACGATTTGCTAAGCCTCATTTTTTAGAAATTATTCTTTTACTATTAGA 77177 0.30215510187462463 No Hit
CCACAATCAAAAGGCAAGGAATTTCGCTACCTTAGCACCCTCACGCTAGG 68128 0.26672742890387585 No Hit
GTCAAATCCGGAAAGAGTTAGTTATTCAGAAAGGTTAGACTTGCTTTCTA 57939 0.22683655036492578 No Hit
CCAGCATAATAATCAATCAAAGAATATCTTAGACAGGTGAACGATTTGCT 55834 0.2185952804341681 No Hit
CTTAAAACTCGAAATTGCATATCACATCCTTAGCAAAGTTTATTCAAGAA 55581 0.21760476200543571 No Hit
CTCATTTTTTAGAAATTATTCTTTTACTATTAGAAAGACAAGTTGTTAAA 55371 0.21678259255866178 No Hit
CTTTCCTAAAAATCTTTTTATTTCGGGAGGTGCTAATACAGCAAAATCTC 54209 0.2122332549531794 No Hit
CCTGATTCAACATCGAGGTGCCAATCCCTTTAGCCAATACGAACTCTACT 52208 0.20439915465320502 No Hit
ATTATACTAAAGGTACTTTTTTTTAACGCTTAAAAACAATGCTTTCATCT 51689 0.20236722159189233 No Hit
GGAGGTGCTAATACAGCAAAATCTCTTGAAAGGCCATTATACTAAAGGTA 51561 0.2018660897386206 No Hit
CTGCTTTCCTAAAAATCTTTTTATTTCGGGAGGTGCTAATACAGCAAAAT 50305 0.19694873342839175 No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTAGACACATCTCGTAT 49835 0.19510863990465965 Illumina Paired End PCR Primer 2 (97% over 35bp)
CTAAAAATCTTTTTATTTCGGGAGGTGCTAATACAGCAAAATCTCTTGAA 48970 0.19172208480247183 No Hit
CAGCAGGAATGCCGAGACCGGTAGACACATCTCGTATGCCGTCTTCTGCT 47739 0.18690260580733517 Illumina PCR Primer Index 9 (96% over 25bp)
CCGGAAAGAGTTAGTTATTCAGAAAGGTTAGACTTGCTTTCTAGGCGCCC 46722 0.18292095662938718 No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44162 0.17289831956395263 No Hit
CTTTCGTACATGCCGAGATAAGTTTATGTAAAGCAGATAGAAACCAACCT 43086 0.1686856799223872 No Hit
CCCACCTAAAAATCTTTTACTCCTGAATAAAGTTAATTTTCAAAGTTGCA 42806 0.1675894539933553 No Hit
CATTATACTAAAGGTACTTTTTTTTAACGCTTAAAAACAATGCTTTCATC 42804 0.16758162380814792 No Hit
CTGGTCTAGTGAGTATCTTTAACACACTACGCCCCACATTAAAGCTGCAC 42428 0.16610954898916225 No Hit
CGGCATAATAATCAATCAAAGAATATCTTAGACAGGTGAACGATTTGCTA 40098 0.15698738322257536 No Hit
GGAAAGAGTTAGTTATTCAGAAAGGTTAGACTTGCTTTCTAGGCGCCCCA 38629 0.15123611218776156 No Hit
CGTAACTTCTCCTATGATCGAATATAATCGGGTCAAATCCGGAAAGAGTT 37665 0.1474619629178089 No Hit
ATTACTAATCCCAGCATAATAATCAATCAAAGAATATCTTAGACAGGTGA 37316 0.14609559559912272 No Hit
CTTAAAAGCATTTTTCGAACAACCAGCTATCTTAAAACTCGAAATTGCAT 36498 0.1428930498493081 No Hit
GCCGAGATAAGTTTATGTAAAGCAGATAGAAACCAACCTGGCTTACGCCG 36296 0.14210220114336364 No Hit
AGCATAATAATCAATCAAAGAATATCTTAGACAGGTGAACGATTTGCTAA 34865 0.1364997036274899 No Hit
GCCCCACCTAAAAATCTTTTACTCCTGAATAAAGTTAATTTTCAAAGTTG 34285 0.1342289499173524 No Hit
CTGCACTTAAAAGCATTTTTCGAACAACCAGCTATCTTAAAACTCGAAAT 33257 0.13020423472076384 No Hit
GGCGTAACTTCTCCTATGATCGAATATAATCGGGTCAAATCCGGAAAGAG 32804 0.12843069777129437 No Hit
ATACTAAAGGTACTTTTTTTTAACGCTTAAAAACAATGCTTTCATCTTAC 31386 0.12287909645926855 No Hit
CCCAGCATAATAATCAATCAAAGAATATCTTAGACAGGTGAACGATTTGC 31220 0.1222291910870568 No Hit
TTTAGAAATTATTCTTTTACTATTAGAAAGACAAGTTGTTAAATACTCAT 31219 0.1222252759944531 No Hit
CTATCTTAAAACTCGAAATTGCATATCACATCCTTAGCAAAGTTTATTCA 30559 0.11964131487602075 No Hit
CCCTGATTCAACATCGAGGTGCCAATCCCTTTAGCCAATACGAACTCTAC 30436 0.11915975848576746 No Hit
CAGCTATCTTAAAACTCGAAATTGCATATCACATCCTTAGCAAAGTTTAT 29226 0.11442249643530819 No Hit
CGGGAGGTGCTAATACAGCAAAATCTCTTGAAAGGCCATTATACTAAAGG 29093 0.11390178911901802 No Hit
TAAATTATCATTCTGCAATTTTTTTCTGGATTTACTCTTTCCAGTCTCAA 28135 0.11015113040468746 No Hit
ACTAAAGGTACTTTTTTTTAACGCTTAAAAACAATGCTTTCATCTTACCA 27537 0.10780990502768362 No Hit
GTAACTTCTCCTATGATCGAATATAATCGGGTCAAATCCGGAAAGAGTTA 26960 0.10555089659535716 No Hit
CCGAATTTTTCACTTATAACATCCTGGTCTAGTGAGTATCTTTAACACAC 26773 0.104818774278468 No Hit
ACTAATCCCAGCATAATAATCAATCAAAGAATATCTTAGACAGGTGAACG 26431 0.10347981260800762 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CAGCA 3973055 4.6237383 72.045555 17
TTTTT 13102565 4.2776337 9.915992 21
GCAGG 2225745 3.9824166 106.55574 19
CCGAG 2125115 3.5045817 96.95281 28
GCCGA 2112545 3.4838521 96.77817 27
AGCAG 2737960 3.4571106 75.5371 18
TGCCG 2248245 3.4304981 89.66732 26
AGACA 3535950 3.150701 53.684128 39
AGCGG 1727480 3.0908954 107.26229 10
GAGCG 1687280 3.0189676 107.47988 9
CCGGT 1940380 2.9607403 88.881935 34
ACCGG 1784215 2.9423947 96.44947 33
GACCG 1779330 2.9343388 96.64358 32
GGAAG 2135905 2.9260764 82.87773 5
GCGGT 1730250 2.8644414 98.9294 11
CAGGA 2218600 2.8013358 75.42985 20
TCAGC 2596875 2.7962725 64.696236 16
CGGAA 2185230 2.7592008 76.41468 4
AAAAA 5719665 2.7537146 5.4872108 36
AGGTG 2154670 2.7311413 11.794129 34
CACAT 3586135 2.7250218 44.880608 42
ATGCC 2524695 2.71855 63.717796 25
GAGAC 2152595 2.717994 74.77729 30
CGAGA 2149465 2.714042 74.44429 29
GGTAG 2093245 2.6532829 74.19412 36
GGAGG 1361630 2.6433089 15.115693 2
ACACA 3210465 2.6366436 48.931522 41
GACAC 2264825 2.6357446 68.65653 40
TAGAC 3072210 2.5328634 48.882984 38
ACATC 3293545 2.5026891 45.188343 43
GGTTC 2313990 2.5013084 64.79124 13
AGGAA 2543135 2.4586005 57.952656 21
AGACC 2108250 2.4535265 68.86056 31
AGAGC 1941920 2.4519832 76.18939 8
GGGAG 1242640 2.4123158 16.87807 1
GGAAT 2678405 2.3958225 53.654846 22
CCAGC 1574805 2.3936632 10.241134 40
AAGAG 2424465 2.3438754 59.159096 7
TTCAG 3047570 2.3247397 46.57998 15
AATGC 2810855 2.317391 49.12986 24
CATCT 3228980 2.2702224 42.053585 44
CTTTC 3481770 2.264973 7.5171366 4
TCTTT 4898045 2.2572405 5.081184 13
AAAGG 2302525 2.2259884 10.09906 30
GATCG 1874925 2.1904323 70.27372 1
ATCGG 1872305 2.1873715 70.2447 2
CGGTA 1864820 2.1786268 68.02381 35
ATCTC 3096030 2.1767485 42.01628 45
AAAGC 2438385 2.1727178 6.2566376 9
GAATG 2428935 2.1726723 53.293625 23
GAAGA 2243575 2.168998 58.777817 6
GCTGC 1403235 2.1411345 9.811405 12
GACAG 1666370 2.1040576 11.458952 31
TCTCG 2111460 2.1036382 58.599686 46
CTGCA 1939505 2.0884273 8.804309 13
GTAGA 2292155 2.0503232 52.62671 37
CAAAG 2292490 2.0427184 8.696215 17
GAAAG 2108110 2.038036 9.876856 29
GGTGC 1227750 2.0325491 11.8387 5
ATCTT 4063030 2.0236962 5.051875 25
CGGTT 1862595 2.0133727 64.314156 12
GTTCA 2614925 1.9947103 45.967457 14
GGCCA 1207015 1.9905194 15.458836 1
TCGGA 1699740 1.9857676 70.3425 3
TCAAA 3376775 1.9646168 5.9975333 12
CAAAA 3037835 1.9102055 5.632764 18
AGCTG 1629605 1.9038306 7.8179603 11
ATCAA 3268300 1.9015056 10.298382 11
TTCTG 2685310 1.8952863 5.254108 13
CAGGT 1619760 1.8923289 10.825144 33
TAAAG 2982135 1.8824375 6.00259 8
TTAAA 4512180 1.8597488 5.7955256 2
CAGCT 1706835 1.8378919 7.5992184 41
CTCTT 2821245 1.8352857 5.405465 24
ACAGC 1576670 1.8348875 8.383475 14
GGTGA 1447105 1.8342712 11.413865 35
ACAGG 1451490 1.8327371 11.285728 32
AAAGA 2684630 1.8315463 6.42307 18
GGCAG 1017370 1.8203304 8.219302 1
GCCTC 1293240 1.8187591 8.181234 15
TCTGG 1678345 1.8142076 5.938539 27
AACAA 2876765 1.8089242 6.3466864 35
CCACA 1685110 1.8075047 10.0373 1
TCTCT 2777535 1.8068513 5.524593 23
TCTTG 2540645 1.793182 5.9259505 25
GCCCC 902530 1.7917007 8.662633 26
CTGGG 1070350 1.7719722 5.030854 1
AATCA 3024690 1.7597728 10.483225 10
AGCAA 1948070 1.7358238 6.7454433 16
AAATC 2978295 1.73278 5.2391024 20
GAGGT 1348395 1.7091516 9.787304 3
TCGGG 1028090 1.7020104 5.0389895 23
CCCAC 1211650 1.6974458 13.028893 1
TCCAG 1551175 1.67028 6.0489035 42
GCAAA 1844945 1.6439345 6.467252 17
CTGGA 1399020 1.6344433 6.3226967 28
AGCAT 1980140 1.6325134 5.8407493 24
AATAA 3651330 1.6265193 5.8811493 7
TTAGA 2772750 1.6194359 6.9986825 28
AAGGT 1808235 1.6174588 6.8123755 14
AAAAC 2555110 1.6066656 5.481538 37
CCCCA 1139085 1.5957867 6.384049 27
CTGCT 1569385 1.5635715 6.0971417 1
AAGCA 1738640 1.5492116 6.08835 23
CTTGA 2017115 1.5386907 6.0238605 26
GCTTT 2179415 1.5382266 5.8309693 45
TTGCT 2169195 1.5310133 7.7519417 41
TCTGC 1529460 1.5237942 6.033312 14
AAGAA 2225605 1.5183839 6.488545 19
ACCAG 1295175 1.507291 7.799165 39
CTGGC 987765 1.5071871 6.0424514 1
AAACA 2392070 1.5041453 5.3661394 38
TGCAC 1388735 1.495367 7.1313405 14
CTGGT 1369355 1.4802047 6.6905456 1
CCTGA 1369100 1.4742246 5.7216353 1
TGCTT 2086000 1.4722944 5.8196936 44
GTGAA 1641250 1.4680914 8.15248 36
CAATC 1896170 1.440856 7.2239227 9
CAATG 1741130 1.435463 6.53403 41
AACCA 1746285 1.4341636 5.701819 38
AGGCC 865770 1.4277636 11.755953 32
CGCCC 715130 1.4196746 8.311365 25
GCCAT 1311815 1.4125409 8.642388 2
CCAGT 1310010 1.4105973 5.4719768 43
GAACA 1577245 1.4054009 6.198318 34
CTTAG 1839220 1.4029893 6.808806 27
TTTGC 1979540 1.3971553 6.8056064 44
TGAAC 1692610 1.3954612 7.3697743 37
GCACT 1294335 1.3937187 7.1432357 15
GATTT 2558875 1.382808 5.120633 42
CGGGA 769280 1.376435 5.9115005 24
GAATA 2167270 1.3680636 6.0779295 21
TGCTA 1788310 1.3641542 6.5675063 42
GTGCT 1258945 1.360857 7.988774 6
GGTAC 1152260 1.3461593 8.604517 16
CCCTC 1013375 1.3135563 5.7322426 38
GCAGA 1036975 1.3093461 7.039229 36
GCTAA 1586410 1.3079053 7.0159154 43
CAACC 1203090 1.2904739 6.9298487 37
TCGAA 1562730 1.2883824 5.4127393 31
TACAG 1560140 1.286247 6.0667934 13
GCATT 1670990 1.2746605 5.3455753 25
AGCCT 1179155 1.2696948 6.3006682 14
TGAAA 2005490 1.2659419 5.0871425 28
GAACG 999335 1.2618196 10.448704 38
CATTA 2340960 1.260171 7.3771214 4
ACATG 1514830 1.2488915 5.138116 11
GCATA 1514610 1.2487103 7.6424665 3
ACAAC 1518195 1.2468411 5.7978234 36
CACAC 1157900 1.2420017 5.0207753 18
AAGCT 1502715 1.2389035 5.8202033 10
AAGGC 980710 1.2383026 9.224086 31
GGGCA 692010 1.2381798 5.933603 1
CCATT 1755495 1.2342489 6.3786945 3
AGAAT 1943720 1.2269504 5.8309064 20
GGCAA 955825 1.2068813 5.796807 1
AGGTA 1345180 1.2032579 6.6798 15
CAGTC 1098970 1.1833528 5.51524 44
ATTTG 2169500 1.1723909 5.048641 43
GCCAG 701985 1.1576616 5.9768095 1
GTGAG 908790 1.1519325 5.751529 4
TCAAT 2128345 1.1457175 5.781565 8
CCGGA 686790 1.132603 6.815846 1
CGAAC 958420 1.1153843 7.2382936 33
AGCAC 954330 1.1106244 5.2046633 34
GCACC 723465 1.0996482 6.4490447 35
GTCTC 1088730 1.0846969 5.0391207 46
ATATC 2010115 1.0820726 5.786377 19
TAATC 1992215 1.0724368 5.9381495 9
AAGCC 918555 1.0689905 9.152805 46
CATAA 1836225 1.0683205 5.271335 4
GTACT 1385775 1.0570933 5.8314967 17
AACGA 1162150 1.0355314 7.5751734 39
TCCGG 678305 1.0349958 5.022658 7
GTCAA 1252360 1.0324999 5.2041564 1
GTCCT 1019725 1.0159476 6.8893323 1
GGCGC 426100 0.99575025 6.130402 44
TACTA 1823830 0.9817928 5.798296 41
CGGCG 420080 0.98168224 6.78329 1
ACGCC 637185 0.96850485 6.4667344 24
CATGC 890155 0.9585042 6.004577 12
CAAGG 758025 0.9571272 5.128364 15
ACGCT 881045 0.94869477 7.681884 30
GCGCC 438135 0.94368994 5.5042195 45
ACGAT 1129850 0.9314974 7.010252 40
CGGCA 563795 0.9297688 7.306675 1
CGCTT 908010 0.9046464 6.881084 31
CGATT 1178680 0.89911777 6.428538 41
ATGCT 1148680 0.8762331 5.5833793 43
CTCGT 868435 0.865218 5.957357 36
CGGCC 394850 0.8504593 5.3226695 1
CCGGC 389380 0.8386775 6.5899243 1
CACCC 597405 0.83692694 5.3019094 36
CGAGG 465150 0.83227015 5.348651 14
CCGGG 353520 0.8261386 6.649989 1
AGCTA 999790 0.82427025 5.130624 42
GCTTA 1076610 0.82125694 5.4079876 32
CGGGC 329450 0.7698895 5.6894703 1
GCCGT 496535 0.7576409 8.785833 43
AACGC 639775 0.74455357 7.9148283 29
CCCGG 344795 0.74264675 5.2879906 1
TAAGC 897050 0.739567 6.3643794 45
CCGTC 517285 0.72748816 8.146908 44
CGTCT 668100 0.6656251 5.943398 45
CTAGG 567500 0.6629975 5.409153 46
CTAAG 783880 0.6462647 6.1845255 44
CGTAC 564515 0.6078605 5.458909 8
CACGC 397240 0.6037946 5.632126 42
TAACG 716055 0.5903468 5.6522756 28
Produced by FastQC (version 0.10.1)